Casas-Mollano, Juan, Jeong, Byeong-Ryool, Xu, Jianping, Moriyama, Hideaki, Cerutti, Heriberto D.
Changes in chromatin organization are emerging as key regulators in nearly every aspect of DNA-templated metabolism in eukaryotes. Histones undergo many, largely reversible, posttranslational...
The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions (2007)
Merchant, Sabeeha S., Prochnik, Simon E., Vallon, Olivier, Harris, Elizabeth H., Karpowicz, Steven J., Witman, George B., ...
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as...
Casas-Mollano, Juan, Van Dijk, Karin, Eisenhart, John, Cerutti, Heriberto D.
SET domain-containing proteins of the SU(VAR)3-9 class are major regulators of heterochromatin in several eukaryotes, including mammals, insects, plants and fungi. The function of these polypeptides...
Untemplated Oligoadenylation Promotes Degradation of RISC-Cleaved Transcripts (2006)
Ibrahim, Fadia, Rohr, Jennifer, Jeong, Won-Joong, Hesson, Jennifer, Cerutti, Heriberto D.
In the best-characterized mechanism of RNAmediated silencing, small interfering RNAs (siRNAs), incorporated into the RNA-induced silencing complex (RISC), guide the endonucleolytic cleavage of...
On the Origin and Functions of RNA-Mediated Silencing: From Protists to Man (2006)
Cerutti, Heriberto D., Casas-Mollano, Juan
Double-stranded RNA has been shown to induce gene silencing in diverse eukaryotes and by a variety of pathways. We have examined the taxonomic distribution and the phylogenetic relationship of key...
Van Dijk, Karen V., Marley, Katherine E., Jeong, Byeong-Ryool, Xu, Jianping, Hesson, Jennifer, Cerny, Ronald, ...
Histone Lys methylation plays an important role in determining chromatin states and is mostly catalyzed by SET domain- containing proteins. The outcome, transcriptional repression or activation,...
Sarkar, Nandita, Lemaire, Stephane, Wu-Scharf, Danxia, Issakidis-Bourguet, Emmanuelle, Cerutti, Heriberto D.
DNA damage occurs as a by-product of intrinsic cellular processes, like DNA replication, or as a consequence of exposure to genotoxic agents. Organisms have evolved multiple mechanisms to avoid,...
Rohr, Jennifer, Sarkar, Nandita, Belanger, Susan, Jeong, Byeong-Ryool, Cerutti, Heriberto D.
RNA interference (RNAi), the double-stranded RNA (dsRNA) triggered post-transcriptional gene silencing, is becoming a powerful tool for reverse genetics studies. Stable RNAi, induced by the...
Ebmeier, A., Allison, L., Cerutti, Heriberto D., Clemente, Thomas E.
The initial step in the synthesis of isoleucine (Ile) is the conversion of threonine to α-ketobutyrate. This reaction is carried out by threonine deaminase (TD), which is feedback-regulated by Ile....
RNA interference: traveling in the cell and gaining functions? (2003)
Double-stranded RNA can induce the degradation of homologous RNAs in organisms as diverse as protozoa, animals, plants and fungi, resulting in post-transcriptional gene silencing. But in some...
Zhang, Chaomei, Wu-Scharf, Dancia, Jeong, Byeong-Ryool, Cerutti, Heriberto D.
In higher plants, mammals, and filamentous fungi, transcriptional gene silencing is frequently associated with DNA methylation. However, recent evidence suggests that certain transgenes can be...
Jeong, Byeong-Ryool, Wu-Scharf, Dancia, Zhang, Chaomei, Cerutti, Heriberto D.
In the unicellular green alga Chlamydomonas reinhardtii, the epigenetic silencing of transgenes occurs, as in land plants, at both the transcriptional and posttranscriptional levels. In the case of...
Transgene and Transposon Silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA Helicase (2000)
Wu-Scharf, Dancia, Jeong, Byeong-Ryool, Zhang, Chaomei, Cerutti, Heriberto D.
The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas...
CDNA ENCODING A RECA HOMOLOG IN EUKARYOTES (1997)
Jagendorf, Andre, Cerutti, Heriberto D.
A nucleotide and deduced amino acid sequence of a cDNA encoding the Arabidopsis thulium RecA protein is disclosed.
Epigenetic Silencing of a Foreign Gene in Nuclear Transformants of Chlamydomonas (1997)
Cerutti, Heriberto D., Johnson, Anita M., Gillham, Nicholas W., Boynton, John E.
The unstable expression of introduced genes poses a serious problem for the application of transgenic technology in plants. In transformants of the unicellular green alga Chlamydomonas reinhardtii,...
Cerutti, Heriberto D., Johnson, A. M., Gillham, N. W., Boynton, J. E.
We have constructed a dominant selectable marker for nuclear transformation of C. reinhardtii, composed of the coding sequence of the eubacterial aadA gene (conferring spectinomycin resistance) fused...
Cerutti, Heriberto D., Johnson, Anita M., Boynton, John E., Gillham, Nicholas W.
Escherichia coli RecA, suggest that the plastid recombination system is related to its eubacterial counterpart. Therefore, we examined whether dominant negative mutants of the E. coli RecA protein...
Cerutti, Heriberto D., Jagendorf, André
Little is known about the mechanistic basis for the movement of promiscuous nucleic acids across cell membranes. To address this problem we sought conditions that would permit the entry of plasmid...
DNA Strand-Transfer Activity in Pea (Pisum sativum L.) Chloroplasts (1993)
Cerutti, Heriberto D., Jagendorf, A. T.
The occurrence of DNA recombination in plastids of higher plants is well documented. However, little is known at the enzymic level. To begin dissecting the biochemical mechanism(s) involved we...
Cerutti, Heriberto D., Ibrahim, H. Z., Jagendorf, A. T.
Organisms must have efficient mechanisms of DNA repair and recombination to prevent alterations in their genetic information due to DNA damage. There is evidence for DNA repair and recombination in...
A Homolog of Escherichia coli RecA Protein in Plastids of Higher Plants (1992)
Osman, H., Grandoni, P., Cerutti, Heriberto D., Jagendorf, André
Studies of chloroplast DNA variations, and several direct experimental observations, indicate the existence of recombination ability in algal and higher plant plastids. However, no studies have been...