Inducible expression of coding and inhibitory RNAs from retargetable genomic loci (2009)
Weidenfeld, Ina, Gossen, Manfred, Löw, Rainer, Kentner, David, Berger, Stefan, Görlich, Dirk, ...
Conditional gene expression systems have developed into essential tools for the study of gene functions. However, their utility is often limited by the difficulty of identifying clonal cell lines, in...
Lucchi, Naomi W, Tongren, Jon, Jain, Vidhan, Nagpal, Avinash C, Kauth, Christian W, Woehlbier, Ute, ...
Abstract Background Plasmodium falciparum infection causes cerebral malaria (CM) in a subset of patients with anti-malarial treatment protecting only about 70% to 80% of patients. Why a subset of...
Benoît Kornmann, Olivier Schaad, Hermann Bujard, Joseph S. Takahashi, Ueli Schibler
The mammalian circadian timing system consists of a master pacemaker in neurons of the suprachiasmatic nucleus (SCN) and clocks of a similar molecular makeup in most peripheral body cells. Peripheral...
Bornkamm, Georg W., Berens, Christian, Kuklik-Roos, Conny, Bechet, Jean-Marie, Laux, Gerhard, Bachl, Jürgen, ...
Conditional expression systems are of pivotal importance for the dissection of complex biological phenomena. Here, we describe a novel EBV-derived episomally replicating plasmid (pRTS-1) that carries...
A genetic switch for epilepsy in adult mice (2004)
Krestel,Heinz Eric, Shimshek,Derya R., Jensen,Vidar, Nevian,Thomas, Kim,Jin−Hyun, Geng,Yu, ...
Premature death from seizures afflicts gene−targeted mice expressing the Q/R site−unedited glutamate receptor subunit GluR−B(Q) of AMPA receptors in central neurons. Early seizure−related...
A genetic switch for epilepsy in adult mice (2004)
Krestel,Heinz Eric, Shimshek,Derya R., Jensen,Vidar, Nevian,Thomas, Kim,Jin−Hyun, Geng,Yu, ...
Premature death from seizures afflicts gene−targeted mice expressing the Q/R site−unedited glutamate receptor subunit GluR−B(Q) of AMPA receptors in central neurons. Early seizure−related...
Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control (2004)
Hasan,Mazahir, Friedrich,Rainer W., Euler,Thomas, Larkum,Matthew E., Giese,Günter, Both,Matthias, ...
Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity−dependent molecular and cellular processes. Great hopes−for the...
Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control (2004)
Hasan,Mazahir, Friedrich,Rainer W., Euler,Thomas, Larkum,Matthew E., Giese,Günter, Both,Matthias, ...
Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity−dependent molecular and cellular processes. Great hopes−for the...
Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control (2004)
Mazahir T. Hasan, Rainer W. Friedrich, Thomas Euler, Matthew E. Larkum, Günter Giese, Matthias Both, ...
Winfred Denk and colleagues succeed in generating transgenic mice that express one of two calcium indicators in their cells, creating a valuable tool to study neuronal activity.
Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control (2004)
Mazahir T. Hasan, Rainer W. Friedrich, Thomas Euler, Matthew E. Larkum, Günter Giese, Matthias Both, ...
Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes—for the...
A genetic switch for epilepsy in adult mice (2004)
Krestel, Heinz Eric, Shimshek, Derya R., Jensen, Vidar, Nevian, Thomas, Kim, Jin−Hyun, Geng, Yu, ...
Premature death from seizures afflicts gene−targeted mice expressing the Q/R site−unedited glutamate receptor subunit GluR−B(Q) of AMPA receptors in central neurons. Early seizure−related...
Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control (2004)
Hasan, Mazahir, Friedrich, Rainer W., Euler, Thomas, Larkum, Matthew E., Giese, Günter, Both, Matthias, ...
Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity−dependent molecular and cellular processes. Great hopes−for the...
Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control (2004)
Hasan, Mazahir, Friedrich, Rainer W., Euler, Thomas, Larkum, Matthew E., Giese, Günter, Both, Matthias, ...
Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity−dependent molecular and cellular processes. Great hopes−for the...
A genetic switch for epilepsy in adult mice (2004)
Krestel, Heinz Eric, Shimshek, Derya R., Jensen, Vidar, Nevian, Thomas, Kim, Jin−Hyun, Geng, Yu, ...
Premature death from seizures afflicts gene−targeted mice expressing the Q/R site−unedited glutamate receptor subunit GluR−B(Q) of AMPA receptors in central neurons. Early seizure−related...
Stringent doxycycline dependent control of CRE recombinase in vivo (2002)
Schönig, Kai, Schwenk, Frieder, Rajewsky, Klaus, Bujard, Hermann
The strategy of modulating gene activities in vivo via CRE/loxP recombination would greatly profit from subjecting the recombination event to an independent and stringent temporal control. Here, we...
Meissner, Markus, Brecht, Susan, Bujard, Hermann, Soldati, Dominique
We have developed a control system for regulating gene activation in Toxoplasma gondii. The elements of this system are derived from the Escherichia coli tetracycline resistance operon, which has...
Lutz, Rolf, Lozinski, Tomasz, Ellinger, Thomas, Bujard, Hermann
The mode of action of regulated promoters is largely determined by kinetic parameters which govern the interaction between promoters and proteins involved in induction and repression of...
Based on parameters governing promoter activity and using regulatory elements of the lac, ara and tet operon transcription control sequences were composed which permit the regulation in Escherichia...
Co-regulation of two gene activities by tetracycline via a bidirectional promoter (1995)
Baron, Udo, Freundlieb, Sabine, Gossen, Manfred, Bujard, Hermann
Bujard, Hermann, Gentz, Reiner, Lanzer, Michael, Stueber, Dietrich, Mueller, Michael, Ibrahimi, Ibrahim, ...
Baron, Udo, Schnappinger, Dirk, Helbl, Vera, Gossen, Manfred, Hillen, Wolfgang, Bujard, Hermann
A regulatory system for the in-depth study of gene functions in higher eukaryotic cells has been developed. It is based on the tetracycline-controlled transactivators and reverse tTA, which were...
Urlinger, Stefanie, Baron, Udo, Thellmann, Marion, Hasan, Mazahir T., Bujard, Hermann, Hillen, Wolfgang
Regulatory elements that control tetracycline resistance in Escherichia coli were previously converted into highly specific transcription regulation systems that function in a wide variety of...
Doxycycline control of prion protein transgene expression modulates prion disease in mice
Tremblay, Patrick, Meiner, Zeev, Galou, Maria, Heinrich, Cornelia, Petromilli, Chris, Lisse, Thomas, ...
Conversion of the cellular prion protein (PrPC) into the pathogenic isoform (PrPSc) is the fundamental event underlying transmission and pathogenesis of prion diseases. To control the expression of...
Lutz, Rolf, Lozinski, Tomasz, Ellinger, Thomas, Bujard, Hermann
The mode of action of regulated promoters is largely determined by kinetic parameters which govern the interaction between promoters and proteins involved in induction and repression of...
Hess, Jochen, Nielsen, Peter J., Fischer, Klaus-Dieter, Bujard, Hermann, Wirth, Thomas
The transcriptional coactivator BOB.1/OBF.1 confers B-cell specificity on the transcription factors Oct1 and Oct2 at octamer site-containing promoters. A hallmark of the BOB.1/OBF.1 mutation in the...
Meissner, Markus, Brecht, Susan, Bujard, Hermann, Soldati, Dominique
We have developed a control system for regulating gene activation in Toxoplasma gondii. The elements of this system are derived from the Escherichia coli tetracycline resistance operon, which has...
Strict Control of Human Immunodeficiency Virus Type 1 Replication by a Genetic Switch: Tet for Tat
Verhoef, Koen, Marzio, Giuseppe, Hillen, Wolfgang, Bujard, Hermann, Berkhout, Ben
Live-attenuated human immunodeficiency virus type 1 (HIV-1) variants have shown great promise as AIDS vaccines, but continued replication can lead to the selection of faster-replicating variants that...
Stringent doxycycline dependent control of CRE recombinase in vivo
Schönig, Kai, Schwenk, Frieder, Rajewsky, Klaus, Bujard, Hermann
The strategy of modulating gene activities in vivo via CRE/loxP recombination would greatly profit from subjecting the recombination event to an independent and stringent temporal control. Here, we...
LOCATION OF SINGLE-STRAND INTERRUPTIONS IN THE DNA OF BACTERIOPHAGE T5+
The positions of three single-strand interruptions in the DNA of phage T5+ have been located by electron microscopy. All three interruptions were found in the same strand. Uneven base composition...
Plasmids Controlling Synthesis of Hemolysin in Escherichia coli: Molecular Properties
Goebel, Werner, Royer-Pokora, Brigitte, Lindenmaier, Werner, Bujard, Hermann
Covalently closed extrachromosomal deoxyribonucleic acid (DNA) was isolated from alpha-hemolytic wild-type strains of Escherichia coli. Most strains examined were able to transfer the hemolytic...
Double-Length, Circular, Single-Stranded DNA from Filamentous Phage
Wheeler, Frances C., Benzinger, Rolf H., Bujard, Hermann
Wild-type and gene 3 mutant filamentous phage stocks, containing different relative amounts of multiple-length particles, were treated exhaustively with DNase and then were highly purified. The phage...
Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control
Hasan, Mazahir T, Friedrich, Rainer W, Euler, Thomas, Larkum, Matthew E, Giese, Günter, Both, Matthias, ...
Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes—for the...
Stringent doxycycline-dependent control of gene activities using an episomal one-vector system
Bornkamm, Georg W., Berens, Christian, Kuklik-Roos, Conny, Bechet, Jean-Marie, Laux, Gerhard, Bachl, Jürgen, ...
Conditional expression systems are of pivotal importance for the dissection of complex biological phenomena. Here, we describe a novel EBV-derived episomally replicating plasmid (pRTS-1) that carries...
Woehlbier, Ute, Epp, Christian, Kauth, Christian W., Lutz, Rolf, Long, Carole A., Coulibaly, Boubacar, ...
The 190-kDa merozoite surface protein 1 (MSP-1) of Plasmodium falciparum, an essential component in the parasite's life cycle, is a primary candidate for a malaria vaccine. Rabbit antibodies elicited...
Tissue-specific expression of ferritin H regulates cellular iron homoeostasis in vivo
Wilkinson, John, Di, Xiumin, Schönig, Kai, Buss, Joan L., Kock, Nancy D., Cline, J. Mark, ...
Ferritin is a ubiquitously distributed iron-binding protein. Cell culture studies have demonstrated that ferritin plays a role in maintenance of iron homoeostasis and in the protection against...
Baron, Udo, Schnappinger, Dirk, Helbl, Vera, Gossen, Manfred, Hillen, Wolfgang, Bujard, Hermann
A regulatory system for the in-depth study of gene functions in higher eukaryotic cells has been developed. It is based on the tetracycline-controlled transactivators and reverse tTA, which were...
Urlinger, Stefanie, Baron, Udo, Thellmann, Marion, Hasan, Mazahir T., Bujard, Hermann, Hillen, Wolfgang
Regulatory elements that control tetracycline resistance in Escherichia coli were previously converted into highly specific transcription regulation systems that function in a wide variety of...
Doxycycline control of prion protein transgene expression modulates prion disease in mice
Tremblay, Patrick, Meiner, Zeev, Galou, Maria, Heinrich, Cornelia, Petromilli, Chris, Lisse, Thomas, ...
Conversion of the cellular prion protein (PrPC) into the pathogenic isoform (PrPSc) is the fundamental event underlying transmission and pathogenesis of prion diseases. To control the expression of...
Lutz, Rolf, Lozinski, Tomasz, Ellinger, Thomas, Bujard, Hermann
The mode of action of regulated promoters is largely determined by kinetic parameters which govern the interaction between promoters and proteins involved in induction and repression of...
Hess, Jochen, Nielsen, Peter J., Fischer, Klaus-Dieter, Bujard, Hermann, Wirth, Thomas
The transcriptional coactivator BOB.1/OBF.1 confers B-cell specificity on the transcription factors Oct1 and Oct2 at octamer site-containing promoters. A hallmark of the BOB.1/OBF.1 mutation in the...
Meissner, Markus, Brecht, Susan, Bujard, Hermann, Soldati, Dominique
We have developed a control system for regulating gene activation in Toxoplasma gondii. The elements of this system are derived from the Escherichia coli tetracycline resistance operon, which has...
Strict Control of Human Immunodeficiency Virus Type 1 Replication by a Genetic Switch: Tet for Tat
Verhoef, Koen, Marzio, Giuseppe, Hillen, Wolfgang, Bujard, Hermann, Berkhout, Ben
Live-attenuated human immunodeficiency virus type 1 (HIV-1) variants have shown great promise as AIDS vaccines, but continued replication can lead to the selection of faster-replicating variants that...
Stringent doxycycline dependent control of CRE recombinase in vivo
Schönig, Kai, Schwenk, Frieder, Rajewsky, Klaus, Bujard, Hermann
The strategy of modulating gene activities in vivo via CRE/loxP recombination would greatly profit from subjecting the recombination event to an independent and stringent temporal control. Here, we...
LOCATION OF SINGLE-STRAND INTERRUPTIONS IN THE DNA OF BACTERIOPHAGE T5+
The positions of three single-strand interruptions in the DNA of phage T5+ have been located by electron microscopy. All three interruptions were found in the same strand. Uneven base composition...
Plasmids Controlling Synthesis of Hemolysin in Escherichia coli: Molecular Properties
Goebel, Werner, Royer-Pokora, Brigitte, Lindenmaier, Werner, Bujard, Hermann
Covalently closed extrachromosomal deoxyribonucleic acid (DNA) was isolated from alpha-hemolytic wild-type strains of Escherichia coli. Most strains examined were able to transfer the hemolytic...
Double-Length, Circular, Single-Stranded DNA from Filamentous Phage
Wheeler, Frances C., Benzinger, Rolf H., Bujard, Hermann
Wild-type and gene 3 mutant filamentous phage stocks, containing different relative amounts of multiple-length particles, were treated exhaustively with DNase and then were highly purified. The phage...
Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control
Hasan, Mazahir T, Friedrich, Rainer W, Euler, Thomas, Larkum, Matthew E, Giese, Günter, Both, Matthias, ...
Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes—for the...
Stringent doxycycline-dependent control of gene activities using an episomal one-vector system
Bornkamm, Georg W., Berens, Christian, Kuklik-Roos, Conny, Bechet, Jean-Marie, Laux, Gerhard, Bachl, Jürgen, ...
Conditional expression systems are of pivotal importance for the dissection of complex biological phenomena. Here, we describe a novel EBV-derived episomally replicating plasmid (pRTS-1) that carries...
Woehlbier, Ute, Epp, Christian, Kauth, Christian W., Lutz, Rolf, Long, Carole A., Coulibaly, Boubacar, ...
The 190-kDa merozoite surface protein 1 (MSP-1) of Plasmodium falciparum, an essential component in the parasite's life cycle, is a primary candidate for a malaria vaccine. Rabbit antibodies elicited...
Tissue-specific expression of ferritin H regulates cellular iron homoeostasis in vivo
Wilkinson, John, Di, Xiumin, Schönig, Kai, Buss, Joan L., Kock, Nancy D., Cline, J. Mark, ...
Ferritin is a ubiquitously distributed iron-binding protein. Cell culture studies have demonstrated that ferritin plays a role in maintenance of iron homoeostasis and in the protection against...
An inducible genetic mouse model for epilepsy
Krestel, Heinz Eric, Nevian, Thomas, Shimshek, Derya R., Kim, Jin-Hyun, Bast, T., Rating, D., ...
Kornmann, Benoît, Schaad, Olivier, Bujard, Hermann, Takahashi, Joseph S, Schibler, Ueli
The mammalian circadian timing system consists of a master pacemaker in neurons of the suprachiasmatic nucleus (SCN) and clocks of a similar molecular makeup in most peripheral body cells. Peripheral...
Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India
Lucchi, Naomi W, Tongren, Jon Eric, Jain, Vidhan, Nagpal, Avinash C, Kauth, Christian W, Woehlbier, Ute, ...
Inducible expression of coding and inhibitory RNAs from retargetable genomic loci
Weidenfeld, Ina, Gossen, Manfred, Löw, Rainer, Kentner, David, Berger, Stefan, Görlich, Dirk, ...
Conditional gene expression systems have developed into essential tools for the study of gene functions. However, their utility is often limited by the difficulty of identifying clonal cell lines, in...
A multifunctional serine protease primes the malaria parasite for red blood cell invasion
Koussis, Konstantinos, Withers-Martinez, Chrislaine, Yeoh, Sharon, Child, Matthew, Hackett, Fiona, Knuepfer, Ellen, ...
The malaria parasite Plasmodium falciparum replicates within an intraerythrocytic parasitophorous vacuole (PV). Rupture of the host cell allows release (egress) of daughter merozoites, which invade...