Hermann Katinger

Unsung Hero Robert C. Gallo (2009)

Abbadessa, Giovanni, Accolla, Roberto, Aiuti, Fernando, Albini, Adriana, Aldovini, Anna, Alfano, Massimo, ...

Awarding the Nobel prize in physiology or medicine to Francoise Barré-Sinoussi and Luc Montagnier for the discovery of HIV-1, the causative agent of AIDS, is timely given the harm that the virus...

Contributions of DNA interstrand cross-links to aging of cells and organisms (2007)

Grillari, Johannes, Katinger, Hermann, Voglauer, Regina

Impaired DNA damage repair, especially deficient transcription-coupled nucleotide excision repair, leads to segmental progeroid syndromes in human patients as well as in rodent models. Furthermore,...

SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly (2005)

Grillari, Johannes, Ajuh, Paul, Stadler, Guido, Löscher, Marlies, Voglauer, Regina, Ernst, Wolfgang, ...

We have isolated the human protein SNEV as downregulated in replicatively senescent cells. Sequence homology to the yeast splicing factor Prp19 suggested that SNEV might be the orthologue of Prp19...

Protection of Macaques against Pathogenic Simian/Human Immunodeficiency Virus 89.6PD by Passive Transfer of Neutralizing Antibodies

Mascola, John R., Lewis, Mark G., Stiegler, Gabriela, Harris, Dawn, VanCott, Thomas C., Hayes, Deborah, ...

The role of antibody in protection against human immunodeficiency virus (HIV-1) has been difficult to study in animal models because most primary HIV-1 strains do not infect nonhuman primates. Using...

Neutralization Sensitivity of Human Immunodeficiency Virus Type 1 Primary Isolates to Antibodies and CD4-Based Reagents Is Independent of Coreceptor Usage

Trkola, Alexandra, Ketas, Tom, KewalRamani, Vineet N., Endorf, Fred, Binley, James M., Katinger, Hermann, ...

We have investigated whether the identity of the coreceptor (CCR5, CXCR4, or both) used by primary human immunodeficiency virus type 1 (HIV-1) isolates to enter CD4+ cells influences the sensitivity...

Synergistic Neutralization of Simian-Human Immunodeficiency Virus SHIV-vpu+ by Triple and Quadruple Combinations of Human Monoclonal Antibodies and High-Titer Anti-Human Immunodeficiency Virus Type 1 Immunoglobulins

Li, An, Katinger, Hermann, Posner, Marshall R., Cavacini, Lisa, Zolla-Pazner, Susan, Gorny, Miroslaw K., ...

We have tested triple and quadruple combinations of human monoclonal antibodies (MAbs), which are directed against various epitopes on human immunodeficiency virus type 1 (HIV-1) envelope...

Transfectant Influenza A Viruses with Long Deletions in the NS1 Protein Grow Efficiently in Vero Cells

Egorov, Andrej, Brandt, Sabine, Sereinig, Sabine, Romanova, Julia, Ferko, Boris, Katinger, Dietmar, ...

We established a reverse genetics system for the nonstructural (NS) gene segment of influenza A virus. This system is based on the use of the temperature-sensitive (ts) reassortant virus 25A-1. The...

Neutralizing Monoclonal Antibodies Block Human Immunodeficiency Virus Type 1 Infection of Dendritic Cells and Transmission to T Cells

Frankel, Sarah S., Steinman, Ralph M., Michael, Nelson L., Kim, Silvia Ratto, Bhardwaj, Nina, Pope, Melissa, ...

Prevention of the initial infection of mucosal dendritic cells (DC) and interruption of the subsequent transmission of HIV-1 from DC to T cells are likely to be important attributes of an effective...

Antibody Neutralization-Resistant Primary Isolates of Human Immunodeficiency Virus Type 1

Wang, Meng, Trkola, Alexandra, Binley, James M., Purtscher, Martin, Katinger, Hermann, ...

Although typical primary isolates of human immunodeficiency virus type 1 (HIV-1) are relatively neutralization resistant, three human monoclonal antibodies and a small number of HIV-1+ human sera...

Hyperattenuated Recombinant Influenza A Virus Nonstructural-Protein-Encoding Vectors Induce Human Immunodeficiency Virus Type 1 Nef-Specific Systemic and Mucosal Immune Responses in Mice

Ferko, Boris, Stasakova, Jana, Sereinig, Sabine, Romanova, Julia, Katinger, Dietmar, Niebler, Brigitte, ...

We have generated recombinant influenza A viruses belonging to the H1N1 and H3N2 virus subtypes containing an insertion of the 137 C-terminal amino acid residues of the human immunodeficiency virus...

Broadly Neutralizing Antibodies Targeted to the Membrane-Proximal External Region of Human Immunodeficiency Virus Type 1 Glycoprotein gp41

Zwick, Michael B., Labrijn, Aran F., Wang, Meng, Spenlehauer, Catherine, Saphire, Erica Ollmann, Binley, James M., ...

The identification and epitope mapping of broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies (Abs) is important for vaccine design, but, despite much effort, very few...

Fine Definition of the Epitope on the gp41 Glycoprotein of Human Immunodeficiency Virus Type 1 for the Neutralizing Monoclonal Antibody 2F5

Parker, Carol E., Deterding, Leesa J., Hager-Braun, Christine, Binley, James M., Schülke, Norbert, Katinger, Hermann, ...

Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), in combination with proteolytic protection assays, has been used to identify the functional epitope on human immunodeficiency...

Postnatal Passive Immunization of Neonatal Macaques with a Triple Combination of Human Monoclonal Antibodies against Oral Simian-Human Immunodeficiency Virus Challenge

Hofmann-Lehmann, Regina, Vlasak, Josef, Rasmussen, Robert A., Smith, Beverly A., Baba, Timothy W., Liska, Vladimir, ...

To develop prophylaxis against mother-to-child human immunodeficiency virus (HIV) transmission, we established a simian-human immunodeficiency virus (SHIV) infection model in neonatal macaques that...

Neutralization Synergy of Human Immunodeficiency Virus Type 1 Primary Isolates by Cocktails of Broadly Neutralizing Antibodies

Zwick, Michael B., Wang, Meng, Poignard, Pascal, Stiegler, Gabriela, Katinger, Hermann, Burton, Dennis R., ...

Several reports have described the existence of synergy between neutralizing monoclonal antibodies (MAbs) against human immunodeficiency virus type 1 (HIV-1). Synergy between human MAbs b12, 2G12,...

The Mannose-Dependent Epitope for Neutralizing Antibody 2G12 on Human Immunodeficiency Virus Type 1 Glycoprotein gp120

Sanders, Rogier W., Venturi, Miro, Schiffner, Linnea, Kalyanaraman, Roopa, Katinger, Hermann, Lloyd, Kenneth O., ...

We have analyzed the unique epitope for the broadly neutralizing human monoclonal antibody (MAb) 2G12 on the gp120 surface glycoprotein of human immunodeficiency virus type 1 (HIV-1). Sequence...

The Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody 2G12 Recognizes a Cluster of α1→2 Mannose Residues on the Outer Face of gp120

Scanlan, Christopher N., Pantophlet, Ralph, Wormald, Mark R., Ollmann Saphire, Erica, Stanfield, Robyn, Wilson, Ian A., ...

2G12 is a broadly neutralizing human monoclonal antibody against human immunodeficiency virus type-1 (HIV-1) that has previously been shown to bind to a carbohydrate-dependent epitope on gp120. Here,...

Characterization of Human Class-Switched Polymeric (Immunoglobulin M [IgM] and IgA) Anti-Human Immunodeficiency Virus Type 1 Antibodies 2F5 and 2G12

Wolbank, Susanne, Kunert, Renate, Stiegler, Gabriela, Katinger, Hermann

We have previously generated human monoclonal anti-human immunodeficiency virus type 1 (anti-HIV-1) antibodies 2F5IgG and 2G12IgG with an exceptional cross-clade neutralizing potential. 2F5IgG and...

Cellular Immunity Elicited by Human Immunodeficiency Virus Type 1/ Simian Immunodeficiency Virus DNA Vaccination Does Not Augment the Sterile Protection Afforded by Passive Infusion of Neutralizing Antibodies

Mascola, John R., Lewis, Mark G., VanCott, Thomas C., Stiegler, Gabriela, Katinger, Hermann, Seaman, Michael, ...

High levels of infused anti-human immunodeficiency virus type 1 (HIV-1) neutralizing monoclonal antibodies (MAbs) can completely protect macaque monkeys against mucosal chimeric simian-human...

The Long Third Complementarity-Determining Region of the Heavy Chain Is Important in the Activity of the Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody 2F5

Zwick, Michael B., Komori, H. Kiyomi, Stanfield, Robyn L., Church, Sarah, Wang, Meng, ...

The human monoclonal antibody 2F5 neutralizes primary human immunodeficiency virus type 1 (HIV-1) with rare breadth and potency. A crystal structure of a complex of 2F5 and a peptide corresponding to...

Effects of Antibody on Viral Kinetics in Simian/Human Immunodeficiency Virus Infection: Implications for Vaccination

Zhang, Lei, Ribeiro, Ruy M., Mascola, John R., Lewis, Mark G., Stiegler, Gabriela, Katinger, Hermann, ...

Passive antibody treatment of macaques prior to simian/human immunodeficiency virus infection produces “sterilizing immunity” in some animals and long-term reductions in viral loads in others....

Structure and Mechanistic Analysis of the Anti-Human Immunodeficiency Virus Type 1 Antibody 2F5 in Complex with Its gp41 Epitope

Ofek, Gilad, Tang, Min, Sambor, Anna, Katinger, Hermann, Mascola, John R., Wyatt, Richard, ...

The membrane-proximal region of the ectodomain of the gp41 envelope glycoprotein of human immunodeficiency virus type 1 (HIV-1) is the target of three of the five broadly neutralizing anti-HIV-1...

Comprehensive Cross-Clade Neutralization Analysis of a Panel of Anti-Human Immunodeficiency Virus Type 1 Monoclonal Antibodies

Binley, James M., Wrin, Terri, Korber, Bette, Zwick, Michael B., Wang, Meng, Chappey, Colombe, ...

Broadly neutralizing monoclonal antibodies (MAbs) are potentially important tools in human immunodeficiency virus type 1 (HIV-1) vaccine design. A few rare MAbs have been intensively studied, but we...

Immunogenicity and Protection Efficacy of Replication-Deficient Influenza A Viruses with Altered NS1 Genes

Ferko, Boris, Stasakova, Jana, Romanova, Julia, Kittel, Christian, Sereinig, Sabine, Katinger, Hermann, ...

We explored the immunogenic properties of influenza A viruses with altered NS1 genes (NS1 mutant viruses). NS1 mutant viruses expressing NS1 proteins with an impaired RNA-binding function or...

Neutralization Profiles of Newly Transmitted Human Immunodeficiency Virus Type 1 by Monoclonal Antibodies 2G12, 2F5, and 4E10

Mehandru, Saurabh, Wrin, Terri, Galovich, Justin, Stiegler, Gabriela, Vcelar, Brigitta, Hurley, Arlene, ...

As the AIDS epidemic continues unabated, the development of a human immunodeficiency virus (HIV) vaccine is critical. Ideally, an effective vaccine should elicit cell-mediated and neutralizing...

Anti-Human Immunodeficiency Virus Type 1 (HIV-1) Antibodies 2F5 and 4E10 Require Surprisingly Few Crucial Residues in the Membrane-Proximal External Region of Glycoprotein gp41 To Neutralize HIV-1

Zwick, Michael B., Jensen, Richard, Church, Sarah, Wang, Meng, Stiegler, Gabriela, Kunert, Renate, ...

The conserved membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 is a target of two broadly neutralizing human monoclonal antibodies, 2F5 and 4E10, and is an...

Interaction of U-box E3 ligase SNEV with PSMB4, the β7 subunit of the 20 S proteasome

Löscher, Marlies, Fortschegger, Klaus, Ritter, Gustav, Wostry, Martina, Voglauer, Regina, Schmid, Johannes A., ...

Recognition of specific substrates for degradation by the ubiquitin–proteasome pathway is ensured by a cascade of ubiquitin transferases E1, E2 and E3. The mechanism by which the target proteins...

Virus Isolates during Acute and Chronic Human Immunodeficiency Virus Type 1 Infection Show Distinct Patterns of Sensitivity to Entry Inhibitors

Rusert, Peter, Kuster, Herbert, Joos, Beda, Misselwitz, Benjamin, Gujer, Cornelia, Leemann, Christine, ...

We studied the effect of entry inhibitors on 58 virus isolates derived during acute and chronic infection to validate these inhibitors in vitro and to probe whether viruses at early and chronic...

Generation of an Influenza A Virus Vector Expressing Biologically Active Human Interleukin-2 from the NS Gene Segment

Kittel, Christian, Ferko, Boris, Kurz, Martina, Voglauer, Regina, Sereinig, Sabine, Romanova, Julia, ...

Engineering of the influenza A virus NS1 protein became an attractive approach to the development of influenza vaccine vectors since it can tolerate large inserts of foreign proteins. However,...

Dissection of the carbohydrate specificity of the broadly neutralizing anti-HIV-1 antibody 2G12

Calarese, Daniel A., Lee, Hing-Ken, Huang, Cheng-Yuan, Best, Michael D., Astronomo, Rena D., Stanfield, Robyn L., ...

Human antibody 2G12 neutralizes a broad range of HIV-1 isolates. Hence, molecular characterization of its epitope, which corresponds to a conserved cluster of oligomannoses on the viral envelope...

SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly

Grillari, Johannes, Ajuh, Paul, Stadler, Guido, Löscher, Marlies, Voglauer, Regina, Ernst, Wolfgang, ...

We have isolated the human protein SNEV as downregulated in replicatively senescent cells. Sequence homology to the yeast splicing factor Prp19 suggested that SNEV might be the orthologue of Prp19...

Long-Term Multiple-Dose Pharmacokinetics of Human Monoclonal Antibodies (MAbs) against Human Immunodeficiency Virus Type 1 Envelope gp120 (MAb 2G12) and gp41 (MAbs 4E10 and 2F5)

Joos, Beda, Trkola, Alexandra, Kuster, Herbert, Aceto, Leonardo, Fischer, Marek, Stiegler, Gabriela, ...

While certain antibodies directed against the human immunodeficiency virus (HIV) envelope have the potential to suppress virus replication in vitro, the impact of neutralizing antibodies in vivo...

Influenza Virus NS Vectors Expressing the Mycobacterium tuberculosis ESAT-6 Protein Induce CD4+ Th1 Immune Response and Protect Animals against Tuberculosis Challenge

Sereinig, Sabine, Stukova, Marina, Zabolotnyh, Natalia, Ferko, Boris, Kittel, Christian, Romanova, Julia, ...

Infection with Mycobacterium tuberculosis remains a major cause of morbidity and mortality all over the world. Since the effectiveness of the only available tuberculosis vaccine, Mycobacterium bovis...

Protection of Macaques against Pathogenic Simian/Human Immunodeficiency Virus 89.6PD by Passive Transfer of Neutralizing Antibodies

Mascola, John R., Lewis, Mark G., Stiegler, Gabriela, Harris, Dawn, VanCott, Thomas C., Hayes, Deborah, ...

The role of antibody in protection against human immunodeficiency virus (HIV-1) has been difficult to study in animal models because most primary HIV-1 strains do not infect nonhuman primates. Using...

Neutralization Sensitivity of Human Immunodeficiency Virus Type 1 Primary Isolates to Antibodies and CD4-Based Reagents Is Independent of Coreceptor Usage

Trkola, Alexandra, Ketas, Tom, KewalRamani, Vineet N., Endorf, Fred, Binley, James M., Katinger, Hermann, ...

We have investigated whether the identity of the coreceptor (CCR5, CXCR4, or both) used by primary human immunodeficiency virus type 1 (HIV-1) isolates to enter CD4+ cells influences the sensitivity...

Synergistic Neutralization of Simian-Human Immunodeficiency Virus SHIV-vpu+ by Triple and Quadruple Combinations of Human Monoclonal Antibodies and High-Titer Anti-Human Immunodeficiency Virus Type 1 Immunoglobulins

Li, An, Katinger, Hermann, Posner, Marshall R., Cavacini, Lisa, Zolla-Pazner, Susan, Gorny, Miroslaw K., ...

We have tested triple and quadruple combinations of human monoclonal antibodies (MAbs), which are directed against various epitopes on human immunodeficiency virus type 1 (HIV-1) envelope...

Transfectant Influenza A Viruses with Long Deletions in the NS1 Protein Grow Efficiently in Vero Cells

Egorov, Andrej, Brandt, Sabine, Sereinig, Sabine, Romanova, Julia, Ferko, Boris, Katinger, Dietmar, ...

We established a reverse genetics system for the nonstructural (NS) gene segment of influenza A virus. This system is based on the use of the temperature-sensitive (ts) reassortant virus 25A-1. The...

Neutralizing Monoclonal Antibodies Block Human Immunodeficiency Virus Type 1 Infection of Dendritic Cells and Transmission to T Cells

Frankel, Sarah S., Steinman, Ralph M., Michael, Nelson L., Kim, Silvia Ratto, Bhardwaj, Nina, Pope, Melissa, ...

Prevention of the initial infection of mucosal dendritic cells (DC) and interruption of the subsequent transmission of HIV-1 from DC to T cells are likely to be important attributes of an effective...

Antibody Neutralization-Resistant Primary Isolates of Human Immunodeficiency Virus Type 1

Wang, Meng, Trkola, Alexandra, Binley, James M., Purtscher, Martin, Katinger, Hermann, ...

Although typical primary isolates of human immunodeficiency virus type 1 (HIV-1) are relatively neutralization resistant, three human monoclonal antibodies and a small number of HIV-1+ human sera...

Hyperattenuated Recombinant Influenza A Virus Nonstructural-Protein-Encoding Vectors Induce Human Immunodeficiency Virus Type 1 Nef-Specific Systemic and Mucosal Immune Responses in Mice

Ferko, Boris, Stasakova, Jana, Sereinig, Sabine, Romanova, Julia, Katinger, Dietmar, Niebler, Brigitte, ...

We have generated recombinant influenza A viruses belonging to the H1N1 and H3N2 virus subtypes containing an insertion of the 137 C-terminal amino acid residues of the human immunodeficiency virus...

Broadly Neutralizing Antibodies Targeted to the Membrane-Proximal External Region of Human Immunodeficiency Virus Type 1 Glycoprotein gp41

Zwick, Michael B., Labrijn, Aran F., Wang, Meng, Spenlehauer, Catherine, Saphire, Erica Ollmann, Binley, James M., ...

The identification and epitope mapping of broadly neutralizing anti-human immunodeficiency virus type 1 (HIV-1) antibodies (Abs) is important for vaccine design, but, despite much effort, very few...

Fine Definition of the Epitope on the gp41 Glycoprotein of Human Immunodeficiency Virus Type 1 for the Neutralizing Monoclonal Antibody 2F5

Parker, Carol E., Deterding, Leesa J., Hager-Braun, Christine, Binley, James M., Schülke, Norbert, Katinger, Hermann, ...

Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), in combination with proteolytic protection assays, has been used to identify the functional epitope on human immunodeficiency...

Postnatal Passive Immunization of Neonatal Macaques with a Triple Combination of Human Monoclonal Antibodies against Oral Simian-Human Immunodeficiency Virus Challenge

Hofmann-Lehmann, Regina, Vlasak, Josef, Rasmussen, Robert A., Smith, Beverly A., Baba, Timothy W., Liska, Vladimir, ...

To develop prophylaxis against mother-to-child human immunodeficiency virus (HIV) transmission, we established a simian-human immunodeficiency virus (SHIV) infection model in neonatal macaques that...

Neutralization Synergy of Human Immunodeficiency Virus Type 1 Primary Isolates by Cocktails of Broadly Neutralizing Antibodies

Zwick, Michael B., Wang, Meng, Poignard, Pascal, Stiegler, Gabriela, Katinger, Hermann, Burton, Dennis R., ...

Several reports have described the existence of synergy between neutralizing monoclonal antibodies (MAbs) against human immunodeficiency virus type 1 (HIV-1). Synergy between human MAbs b12, 2G12,...

The Mannose-Dependent Epitope for Neutralizing Antibody 2G12 on Human Immunodeficiency Virus Type 1 Glycoprotein gp120

Sanders, Rogier W., Venturi, Miro, Schiffner, Linnea, Kalyanaraman, Roopa, Katinger, Hermann, Lloyd, Kenneth O., ...

We have analyzed the unique epitope for the broadly neutralizing human monoclonal antibody (MAb) 2G12 on the gp120 surface glycoprotein of human immunodeficiency virus type 1 (HIV-1). Sequence...

The Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody 2G12 Recognizes a Cluster of α1→2 Mannose Residues on the Outer Face of gp120

Scanlan, Christopher N., Pantophlet, Ralph, Wormald, Mark R., Ollmann Saphire, Erica, Stanfield, Robyn, Wilson, Ian A., ...

2G12 is a broadly neutralizing human monoclonal antibody against human immunodeficiency virus type-1 (HIV-1) that has previously been shown to bind to a carbohydrate-dependent epitope on gp120. Here,...

Characterization of Human Class-Switched Polymeric (Immunoglobulin M [IgM] and IgA) Anti-Human Immunodeficiency Virus Type 1 Antibodies 2F5 and 2G12

Wolbank, Susanne, Kunert, Renate, Stiegler, Gabriela, Katinger, Hermann

We have previously generated human monoclonal anti-human immunodeficiency virus type 1 (anti-HIV-1) antibodies 2F5IgG and 2G12IgG with an exceptional cross-clade neutralizing potential. 2F5IgG and...

Cellular Immunity Elicited by Human Immunodeficiency Virus Type 1/ Simian Immunodeficiency Virus DNA Vaccination Does Not Augment the Sterile Protection Afforded by Passive Infusion of Neutralizing Antibodies

Mascola, John R., Lewis, Mark G., VanCott, Thomas C., Stiegler, Gabriela, Katinger, Hermann, Seaman, Michael, ...

High levels of infused anti-human immunodeficiency virus type 1 (HIV-1) neutralizing monoclonal antibodies (MAbs) can completely protect macaque monkeys against mucosal chimeric simian-human...

The Long Third Complementarity-Determining Region of the Heavy Chain Is Important in the Activity of the Broadly Neutralizing Anti-Human Immunodeficiency Virus Type 1 Antibody 2F5

Zwick, Michael B., Komori, H. Kiyomi, Stanfield, Robyn L., Church, Sarah, Wang, Meng, ...

The human monoclonal antibody 2F5 neutralizes primary human immunodeficiency virus type 1 (HIV-1) with rare breadth and potency. A crystal structure of a complex of 2F5 and a peptide corresponding to...

Effects of Antibody on Viral Kinetics in Simian/Human Immunodeficiency Virus Infection: Implications for Vaccination

Zhang, Lei, Ribeiro, Ruy M., Mascola, John R., Lewis, Mark G., Stiegler, Gabriela, Katinger, Hermann, ...

Passive antibody treatment of macaques prior to simian/human immunodeficiency virus infection produces “sterilizing immunity” in some animals and long-term reductions in viral loads in others....

Structure and Mechanistic Analysis of the Anti-Human Immunodeficiency Virus Type 1 Antibody 2F5 in Complex with Its gp41 Epitope

Ofek, Gilad, Tang, Min, Sambor, Anna, Katinger, Hermann, Mascola, John R., Wyatt, Richard, ...

The membrane-proximal region of the ectodomain of the gp41 envelope glycoprotein of human immunodeficiency virus type 1 (HIV-1) is the target of three of the five broadly neutralizing anti-HIV-1...

Comprehensive Cross-Clade Neutralization Analysis of a Panel of Anti-Human Immunodeficiency Virus Type 1 Monoclonal Antibodies

Binley, James M., Wrin, Terri, Korber, Bette, Zwick, Michael B., Wang, Meng, Chappey, Colombe, ...

Broadly neutralizing monoclonal antibodies (MAbs) are potentially important tools in human immunodeficiency virus type 1 (HIV-1) vaccine design. A few rare MAbs have been intensively studied, but we...

Immunogenicity and Protection Efficacy of Replication-Deficient Influenza A Viruses with Altered NS1 Genes

Ferko, Boris, Stasakova, Jana, Romanova, Julia, Kittel, Christian, Sereinig, Sabine, Katinger, Hermann, ...

We explored the immunogenic properties of influenza A viruses with altered NS1 genes (NS1 mutant viruses). NS1 mutant viruses expressing NS1 proteins with an impaired RNA-binding function or...

Neutralization Profiles of Newly Transmitted Human Immunodeficiency Virus Type 1 by Monoclonal Antibodies 2G12, 2F5, and 4E10

Mehandru, Saurabh, Wrin, Terri, Galovich, Justin, Stiegler, Gabriela, Vcelar, Brigitta, Hurley, Arlene, ...

As the AIDS epidemic continues unabated, the development of a human immunodeficiency virus (HIV) vaccine is critical. Ideally, an effective vaccine should elicit cell-mediated and neutralizing...

Anti-Human Immunodeficiency Virus Type 1 (HIV-1) Antibodies 2F5 and 4E10 Require Surprisingly Few Crucial Residues in the Membrane-Proximal External Region of Glycoprotein gp41 To Neutralize HIV-1

Zwick, Michael B., Jensen, Richard, Church, Sarah, Wang, Meng, Stiegler, Gabriela, Kunert, Renate, ...

The conserved membrane-proximal external region (MPER) of human immunodeficiency virus type 1 (HIV-1) gp41 is a target of two broadly neutralizing human monoclonal antibodies, 2F5 and 4E10, and is an...

Interaction of U-box E3 ligase SNEV with PSMB4, the β7 subunit of the 20 S proteasome

Löscher, Marlies, Fortschegger, Klaus, Ritter, Gustav, Wostry, Martina, Voglauer, Regina, Schmid, Johannes A., ...

Recognition of specific substrates for degradation by the ubiquitin–proteasome pathway is ensured by a cascade of ubiquitin transferases E1, E2 and E3. The mechanism by which the target proteins...

Virus Isolates during Acute and Chronic Human Immunodeficiency Virus Type 1 Infection Show Distinct Patterns of Sensitivity to Entry Inhibitors

Rusert, Peter, Kuster, Herbert, Joos, Beda, Misselwitz, Benjamin, Gujer, Cornelia, Leemann, Christine, ...

We studied the effect of entry inhibitors on 58 virus isolates derived during acute and chronic infection to validate these inhibitors in vitro and to probe whether viruses at early and chronic...

Generation of an Influenza A Virus Vector Expressing Biologically Active Human Interleukin-2 from the NS Gene Segment

Kittel, Christian, Ferko, Boris, Kurz, Martina, Voglauer, Regina, Sereinig, Sabine, Romanova, Julia, ...

Engineering of the influenza A virus NS1 protein became an attractive approach to the development of influenza vaccine vectors since it can tolerate large inserts of foreign proteins. However,...

Dissection of the carbohydrate specificity of the broadly neutralizing anti-HIV-1 antibody 2G12

Calarese, Daniel A., Lee, Hing-Ken, Huang, Cheng-Yuan, Best, Michael D., Astronomo, Rena D., Stanfield, Robyn L., ...

Human antibody 2G12 neutralizes a broad range of HIV-1 isolates. Hence, molecular characterization of its epitope, which corresponds to a conserved cluster of oligomannoses on the viral envelope...

SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly

Grillari, Johannes, Ajuh, Paul, Stadler, Guido, Löscher, Marlies, Voglauer, Regina, Ernst, Wolfgang, ...

We have isolated the human protein SNEV as downregulated in replicatively senescent cells. Sequence homology to the yeast splicing factor Prp19 suggested that SNEV might be the orthologue of Prp19...

Long-Term Multiple-Dose Pharmacokinetics of Human Monoclonal Antibodies (MAbs) against Human Immunodeficiency Virus Type 1 Envelope gp120 (MAb 2G12) and gp41 (MAbs 4E10 and 2F5)

Joos, Beda, Trkola, Alexandra, Kuster, Herbert, Aceto, Leonardo, Fischer, Marek, Stiegler, Gabriela, ...

While certain antibodies directed against the human immunodeficiency virus (HIV) envelope have the potential to suppress virus replication in vitro, the impact of neutralizing antibodies in vivo...

Influenza Virus NS Vectors Expressing the Mycobacterium tuberculosis ESAT-6 Protein Induce CD4+ Th1 Immune Response and Protect Animals against Tuberculosis Challenge

Sereinig, Sabine, Stukova, Marina, Zabolotnyh, Natalia, Ferko, Boris, Kittel, Christian, Romanova, Julia, ...

Infection with Mycobacterium tuberculosis remains a major cause of morbidity and mortality all over the world. Since the effectiveness of the only available tuberculosis vaccine, Mycobacterium bovis...

Live Attenuated Influenza Virus Expressing Human Interleukin-2 Reveals Increased Immunogenic Potential in Young and Aged Hosts▿

Ferko, Boris, Kittel, Christian, Romanova, Julia, Sereinig, Sabine, Katinger, Hermann, Egorov, Andrej

Despite the reported efficacy of commercially available influenza virus vaccines, a considerable proportion of the human population does not respond well to vaccination. In an attempt to improve the...

Early Embryonic Lethality of Mice Lacking the Essential Protein SNEV▿ ‡

Fortschegger, Klaus, Wagner, Bettina, Voglauer, Regina, Katinger, Hermann, Sibilia, Maria, Grillari, Johannes

SNEV (Prp19, Pso4, NMP200) is a nuclear matrix protein known to be involved in pre-mRNA splicing, ubiquitylation, and DNA repair. In human umbilical vein endothelial cells, SNEV overexpression...

In Vivo and In Vitro Escape from Neutralizing Antibodies 2G12, 2F5, and 4E10▿

Manrique, Amapola, Rusert, Peter, Joos, Beda, Fischer, Marek, Kuster, Herbert, Leemann, Christine, ...

Recently, passive immunization of human immunodeficiency virus (HIV)-infected individuals with monoclonal antibodies (MAbs) 2G12, 2F5, and 4E10 provided evidence of the in vivo activity of 2G12 but...

Adjunctive Passive Immunotherapy in Human Immunodeficiency Virus Type 1-Infected Individuals Treated with Antiviral Therapy during Acute and Early Infection▿ †

Mehandru, Saurabh, Vcelar, Brigitta, Wrin, Terri, Stiegler, Gabriela, Joos, Beda, Mohri, Hiroshi, ...

Three neutralizing monoclonal antibodies (MAbs), 2G12, 2F5, and 4E10, with activity in vitro and in vivo were administered in an open-label, nonrandomized, proof-of-concept study to attempt to...

Contributions of DNA interstrand cross-links to aging of cells and organisms

Grillari, Johannes, Katinger, Hermann, Voglauer, Regina

Impaired DNA damage repair, especially deficient transcription-coupled nucleotide excision repair, leads to segmental progeroid syndromes in human patients as well as in rodent models. Furthermore,...

In Vivo Efficacy of Human Immunodeficiency Virus Neutralizing Antibodies: Estimates for Protective Titers▿

Trkola, Alexandra, Kuster, Herbert, Rusert, Peter, Von Wyl, Viktor, Leemann, Christine, Weber, Rainer, ...

The definition of plasma neutralizing antibody titers capable of controlling human immunodeficiency virus (HIV) infection in vivo is considered a critical step in vaccine development. Here we provide...

Potent Human Immunodeficiency Virus-Neutralizing and Complement Lysis Activities of Antibodies Are Not Obligatorily Linked▿ †

Huber, Michael, Von Wyl, Viktor, Ammann, Christoph G., Kuster, Herbert, Stiegler, Gabriela, Katinger, Hermann, ...

To evaluate the contribution of complement-mediated lysis to the in vivo activities of neutralizing antibodies, we analyzed the influence of complement activation on treatment success in a recent...

Serum-free transfection of CHO-cells with tailor-made unilamellar vesicles

Reisinger, Hannes, Sevcsik, Eva, Vorauer-Uhl, Karola, Lohner, Karl, Katinger, Hermann, Kunert, Renate

At present, a number of transfection techniques are available to introduce foreign DNA into cells, but still minimal intrusion or interference with normal cell physiology, low toxicity,...

Broad Neutralization of Human Immunodeficiency Virus Type 1 (HIV-1) Elicited from Human Rhinoviruses That Display the HIV-1 gp41 ELDKWA Epitope▿ †

Arnold, Gail Ferstandig, Velasco, Paola K., Holmes, Andrew K., Wrin, Terri, Geisler, Sheila C., Phung, Pham, ...

In efforts to develop AIDS vaccine components, we generated combinatorial libraries of recombinant human rhinoviruses that display the well-conserved ELDKWA epitope of the membrane-proximal external...