Runck, Amy M., Moriyama, Hideaki, Storz, Jay F.
The functional diversification of multigene families may be strongly influenced by mechanisms of concerted evolution such as interparalog gene conversion. The β-globin gene family of house mice...
Nakachi, Mia, Matsumoto, Midori, Terry, Philip M., Cerny, Ronald L., Moriyama, Hideaki
Marine invertebrates employ external fertilization to take the advantages of sexual reproduction as one of excellent survival strategies. To prevent mismatching, successful fertilization can be made...
Casas-Mollano, Juan, Jeong, Byeong-Ryool, Xu, Jianping, Moriyama, Hideaki, Cerutti, Heriberto D.
Changes in chromatin organization are emerging as key regulators in nearly every aspect of DNA-templated metabolism in eukaryotes. Histones undergo many, largely reversible, posttranslational...
Adaptive Functional Divergence among Triplicated α-Globin Genes in Rodents (2008)
Storz, Jay F., Hoffmann, Federico G., Opazo, Juan C., Moriyama, Hideaki
The functional divergence of duplicated genes is thought to play an important role in the evolution of new developmental and physiological pathways, but the role of positive selection in driving this...
Adaptive Functional Divergence among Triplicated α-Globin Genes in Rodents (2008)
Storz, Jay F., Hoffmann, Federico G., Opazo, Juan C., Moriyama, Hideaki
The functional divergence of duplicated genes is thought to play an important role in the evolution of new developmental and physiological pathways, but the role of positive selection in driving this...
Bajaj, Mamta, Moriyama, Hideaki
The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion...
Supporting Information for "The Molecular Basis of High-Altitude Adaptation in Deer Mice" (2007)
Storz, Jay F., Sabatino, Stephen J., Hoffmann, Federico G., Gering, Eben J., Moriyama, Hideaki, Ferrand, Nuno, ...
Figure S1: Variation in Site-Specific Levels of Altitudinal Differentiation across the 5′ α- Globin Gene of P. maniculatus Figure S2: Relationship between Pairwise Linkage Disequilibrium and...
The Molecular Basis of High-Altitude Adaptation in Deer Mice (2007)
Storz, Jay F., Sabatino, Stephen J., Hoffmann, Federico G., Gering, Eben J., Moriyama, Hideaki, Ferrand, Nuno, ...
Elucidating genetic mechanisms of adaptation is a goal of central importance in evolutionary biology, yet few empirical studies have succeeded in documenting causal links between molecular variation...
The Molecular Basis of High-Altitude Adaptation in Deer Mice (2007)
Jay F. Storz, Stephen J. Sabatino, Federico G. Hoffmann, Eben J. Gering, Hideaki Moriyama, Nuno Ferrand, ...
Elucidating genetic mechanisms of adaptation is a goal of central importance in evolutionary biology, yet few empirical studies have succeeded in documenting causal links between molecular variation...
The Molecular Basis of High Altitude Adaptation in Deer Mice (2007)
Jay F. Storz, Stephen J. Sabatino, Federico G. Hoffmann, Eben J. Gering, Hideaki Moriyama, Nuno Ferrand, ...
Elucidating genetic mechanisms of adaptation is a goal of central importance in evolutionary biology, yet few empirical studies have succeeded in documenting causal links between molecular variation...
Moriyama, Hideaki, Sugi, Norio, Kawasaki, Kazunori, Muramatsu, Shoji
An apparatus for growing a biological macromolecular crystal by vaporizing biological macromolecular solution into an oversaturated state. The apparatus includes a first sealed room that receives...
Acrosome reaction is subfamily specific in sea star fertilization (2006)
Nakachi, Mia, Moriyama, Hideaki, Hoshi, Motonori, Matsumoto, Midori
In the fertilization process of sea stars, sperm is activated to go through the acrosome reaction before cell fusion. We focused on induction of the acrosome reaction as a key process in...
Xu, Wenxin, Ahmed, Shaheen, Moriyama, Hideaki, Chollet, Raymond
Phosphoenolpyruvate carboxylase (PEPC) is a "multifaceted," allosteric enzyme involved in C4 acid metabolism in green plants/microalgae and prokaryotes. Before the elucidation of the...
Alvarez-Venegas, Raul, Sadder, Monther, Hlavacka, Andrej, Baluska, Frantisek, Xia, Yuannan, Lu, Guoqing, ...
TheArabidopsis homolog of trithorax, ATX1, regulates numerous functions inArabidopsis beyond the homeotic genes. Here, we identified genome-wide targets of ATX1 and showed that ATX1 is a receptor for...
Chlorella Virus-Encoded Deoxyuridine Triphosphatases Exhibit Different Temperature Optima (2005)
Zhang, Yuanzheng, Moriyama, Hideaki, Homma, Kohei, Van Etten, James L.
A putative deoxyuridine triphosphatase (dUTPase) gene from chlorella virus PBCV-1 was cloned, and the recombinant protein was expressed in Escherichia coli. The recombinant protein has dUTPase...
Chatani, Eri, Hayashi, Rikimaru, Moriyama, Hideaki, Ueki, Tatzuo
The replacement of Phe120 with other hydrophobic residues causes a decrease in the activity and thermal stability in ribonuclease A (RNase A). To explain this, the crystal structures of wild-type...
Crystal structures of mutants of Thermus thermophilus IPMDH adapted to low temperatures (2001)
Hirose, Raita, Suzuki, Toshiharu, Moriyama, Hideaki, Sato, Takao, Yamagishi, Akihiko, Oshima, Tairo, ...
Random mutagenesis on thermophilic 3-isopropylmalate dehydrogenases (IPMDH; EC 1.1.1.85) produced mutant enzymes which adapt to low temperatures. These mutants had higher activity at lower...
Hori, Tetsuya, Moriyama, Hideaki, Kawaguchi, Jitsutaro, Hayashi-Iwasaki, Yoko, Oshima, Tairo, Tanaka, Nobuo
A temperature-jump (T-jump) time-resolved X-ray crystallographic technique using the Laue method was developed to detect small, localized structural changes of proteins in crystals exposed to a...
Crystal Structure of H2-Proteinase from the Venom of Trimeresurus flavoviridis (1996)
Kumasaka, Takashi, Yamamoto, Masaki, Moriyama, Hideaki, Tanaka, Nobuo, Sato, Mamoru, Katsube, Yukiteru, ...
The crystal structure of the zinc-protease, H2-proteinase, isolated from the venom of Trimeresurus flavoviridis has been determined. The crystallographic R factor is 0.176 for 10,635 reflections with...
Ohzeki, Masato, Yaoi, Takuro, Moriyama, Hideaki, Oshima, Tairo, Tanaka, Nobuo
Isocitrate dehydrogenase from the thermophilic bacterium, Thermus thermophilus HB8, was crystallized by the vapor diffusion hanging drop method with polyethylene glycol 6000 as the precipitant....
Kadono, Shojiro, Sakurai, Masahiro, Moriyama, Hideaki, Sato, Mamoru, Hayashi, Yoko, Oshima, Tairo, ...
The structures of 3-isopropylmalate dehydrogenase (IPMDH) from Thermus thermophilus in complexes with its substrate, cofactor, and a cofactor analog were investigated by X-ray diffraction in a...
Moriyama, Hideaki, Onodera, Ko, Sakurai, Masahiro, Tanaka, Nobuo, Kirino, Hiromi, Oshima, Tairo, ...
The structures of two mutant forms (G240A and L246E/V249M) of 3-isopropylmalate dehydrogenase from Thermus thermophilus HB8 were studied by X-ray crystallography. In the case of G240A, the...
Kumasaka, Takashi, Takeya, Hiroyuki, Yamamoto, Masaki, Yamakawa, Yoshio, Omori-Satoh, Tamotsu, Moriyama, Hideaki, ...
H2-proteinase, a non-hemorrhagic metalloproteinase from the venom of Trimeresurus flavoviridis, has been crystallized by vapor diffusion from solutions containing ammonium sulfate. The crystals...
Crystallization and Preliminary X-Ray Analysis of Cytochrome c-554 from Nitrosomonas europaea (1995)
Nagata, Chikahiro, Igarashi, Noriyuki, Moriyama, Hideaki, Fujiwara, Taketomo, Fukumori, Yoshihiro, Tanaka, Nobuo
Cytochrome c-554 from Nitrosomonas europaea was crystallized by the batch method utilizing ammonium sulfate as the precipitant. The crystals belong to the space group, P21, with unit cell dimensions...
Onodera, Ko, Sakurai, Masahiro, Moriyama, Hideaki, Tanaka, Nobuo, Numata, Koichi, Oshima, Tairo, ...
The 3-D structures of two chimeric enzymes (4M6T and 2T2M6T) between the Bacillus subtilis and Thermus thermophilus 3-isopropylmalate dehydrogenases were analysed by X-ray diffraction in order to...
Crystallization and Preliminary X-Ray Analysis of Cytochrome c-552 from Nitrosomonas europaea (1994)
Nagata, Chikahiro, Moriyama, Hideaki, Fujiwara, Taketomo, Fukumori, Yoshihiro, Tanaka, Nobuo
Cytochrome c-552 from Nitrosomonas europaea was crystallized by the sandwiched drop, vapor diffusion method, with anmmonium sulfate as the precipitant. The crystals were found to belong to the space...
Miyazaki, Kentaro, Kadono, Shojiro, Sakurai, Masahiro, Moriyama, Hideaki, Tanaka, Nobuo, Oshima, Tairo
3-Isopropylmalate dehydrogenase from an extreme thermo-phile, Thermus thermophUus HB8, was chemically modified with tetranitromethane which nitrated 1.5-2.0 Tyr residues per subunit. The nitration...
Sakurai, Masahiro, Onodera, Ko, Moriyama, Hideaki, Matsumoto, Osamau, Tanaka, Nobuo, Numata, Koichi, ...
A new type of chimeric 3-isopropylmalate dehydrogenase (2T2M6T) was produced by expressing the fused gene of Bacillus subtilis and Thermus thermophilus. The enzyme shows heat stability intermediate...
Crystallization and Preliminary X-Ray Studies on Sulfolobus acidocaldarius Ferredoxin (1991)
Fujii, Tomomi, Moriyama, Hideaki, Takenaka, Akio, Tanaka, Nobuo, Wakagi, Takayoshi, Oshima, Tairo
Ferredoxin from a thermoacidophilic archaebacterium, Sulfolobus acidocaldarius, has been crystallized. The space group is P432l2 or P43212 and the cell dimensions are a=b = 50.12 � and c = 69.52...
Mikami, Toshiyuki, Tanaka, Nobuo, Sato, Takao, Moriyama, Hideaki, Numata, Masahiko, Fujiwara, Taketomo, ...
Hydroxylamine oxidoreductase [EC 1.7.3.4] from Nitrosomonas europaea was crystallized by the microdialysis method using ammonium sulfate. Its space group is P63 with cell dimensions of a= 6 b= 96.4...
Chlorella Virus-Encoded Deoxyuridine Triphosphatases Exhibit Different Temperature Optima
Zhang, Yuanzheng, Moriyama, Hideaki, Homma, Kohei, Van Etten, James L.
A putative deoxyuridine triphosphatase (dUTPase) gene from chlorella virus PBCV-1 was cloned, and the recombinant protein was expressed in Escherichia coli. The recombinant protein has dUTPase...
Alvarez-Venegas, Raul, Sadder, Monther, Hlavacka, Andrej, Baluška, František, Xia, Yuannan, Lu, Guoqing, ...
The Arabidopsis homolog of trithorax, ATX1, regulates numerous functions in Arabidopsis beyond the homeotic genes. Here, we identified genome-wide targets of ATX1 and showed that ATX1 is a receptor...
Chlorella Virus-Encoded Deoxyuridine Triphosphatases Exhibit Different Temperature Optima
Zhang, Yuanzheng, Moriyama, Hideaki, Homma, Kohei, Van Etten, James L.
A putative deoxyuridine triphosphatase (dUTPase) gene from chlorella virus PBCV-1 was cloned, and the recombinant protein was expressed in Escherichia coli. The recombinant protein has dUTPase...
Alvarez-Venegas, Raul, Sadder, Monther, Hlavacka, Andrej, Baluška, František, Xia, Yuannan, Lu, Guoqing, ...
The Arabidopsis homolog of trithorax, ATX1, regulates numerous functions in Arabidopsis beyond the homeotic genes. Here, we identified genome-wide targets of ATX1 and showed that ATX1 is a receptor...
The Molecular Basis of High-Altitude Adaptation in Deer Mice
Storz, Jay F, Sabatino, Stephen J, Hoffmann, Federico G, Gering, Eben J, Moriyama, Hideaki, Ferrand, Nuno, ...
Elucidating genetic mechanisms of adaptation is a goal of central importance in evolutionary biology, yet few empirical studies have succeeded in documenting causal links between molecular variation...
Chatani, Eri, Hayashi, Rikimaru, Moriyama, Hideaki, Ueki, Tatzuo
The replacement of Phe120 with other hydrophobic residues causes a decrease in the activity and thermal stability in ribonuclease A (RNase A). To explain this, the crystal structures of wild-type...
Adaptive Functional Divergence Among Triplicated α-Globin Genes in Rodents
Storz, Jay F., Hoffmann, Federico G., Opazo, Juan C., Moriyama, Hideaki
The functional divergence of duplicated genes is thought to play an important role in the evolution of new developmental and physiological pathways, but the role of positive selection in driving this...
Casas-Mollano, J. Armando, Jeong, Byeong-ryool, Xu, Jianping, Moriyama, Hideaki, Cerutti, Heriberto
Changes in chromatin organization are emerging as key regulators in nearly every aspect of DNA-templated metabolism in eukaryotes. Histones undergo many, largely reversible, posttranslational...
Bajaj, Mamta, Moriyama, Hideaki
The first crystallization of deoxyuridine triphosphate nucleotidohydrolase from plant, Arabidopsis thaliana, has been performed. An additive, taurine, was effective in producing the single crystal.
Crystallization and crystal-packing studies of Chlorella virus deoxyuridine triphosphatase
Homma, Kohei, Moriyama, Hideaki
Algal Chlorella virus IL-3A deoxyuridine triphosphatase and its Glu81Ser plus Thr84Arg-mutated derivative, Mu-22, were crystallized using the hanging-drop vapor-diffusion method. Glycyl-seryl-tagged...