Observations on Cryptosporidium Life Cycle Stages During Excystation (2008)
Panagiotis Karanis, Akio Kimura, Hideyuki Nagasawa, Ikuo Igarashi, Naoyoshi Suzuki
Cryptosporidium parvum (HNJ-1 strain, genotype 2) merozoites were released from oocysts directly during an incubation and excystation procedure without bleach treatment. They were polymorphic, mostly...
Hiroaki Shirafuji, Xuenan Xuan, Isao Kimata, Yasuhiro Takashima, Shinya Fukumoto, Haruki Otsuka, ...
In this study, P23 of Cryptosporidium parvum sporozoites, an immunodominant surface protein, was stably expressed in Toxoplasma gondii (Tg/P23) and its protective effects were evaluated in a mouse...
Shinya Fukumoto, Yukiko Sekine, Xuenan Xuan, Ikuo Igarashi, Chihiro Sugimoto, Hideyuki Nagasawa, ...
The entire P50 gene encoding a surface protein of Babesia gibsoni was cloned into the bacteria expression vector pGEX-4T-3 and subsequently expressed in Escherichia coli as a glutathione...
Yasuhiro Takashima, Xuenan Xuan, Isao Kimata, Motohiro Iseki, Yoshikatsu Kodama, Noriko Nagane, ...
In order to develop a vaccine against cryptosporidiosis in cattle, we constructed a recombinant bovine herpesvirus-1 (BHV-1) expressing an immunodominant surface protein, p23, of Cryptosporidium...
Badgar Battsetseg, Kamilius Mamiro, Noboru Inoue, Levi Makala, Hideyuki Nagasawa, Yoichiro Iwakura, ...
To investigate the immunological mechanisms of acquired resistance to tick infestation, interferon gamma (IFN-γ) deficient mice (IFN-γ −/− mice) were used to assess interleukin-4 (IL-4) and...
Characterization of Phagocytic Hemocytes in Ornithodoros moubata (Acari: Ixodidae) (2001)
Noboru Inoue, Kayoko Hanada, Naotoshi Tsuji, Ikuo Igarashi, Hideyuki Nagasawa, Takeshi Mikami, ...
Effects of fetal bovine serum (FBS) and complement on phagocytic activity in Ornithodoros moubata (Murray 1877) hemocytes and protease activity in the hemocytes were examined. At least three...
Shinya Fukumoto, Xuenan Xuan, Ikuo Igarashi, Shoufa Zhang, Jennifer Mugisha, Takashi Ogata, ...
Canine red blood cell-substituted severe combined immune deficiency (Ca-RBC-SCID) mice were prepared for canine Babesia gibsoni infection. The Ca-RBC-SCID mice infected with B. gibsoni developed a...
Ikadai, Hiromi, Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Shigeyasu, Kanemaru, Takumi, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia caballi merozoite mRNA was screened with a monoclonal antibody BC11D against the rhoptry protein of B. caballi merozoite. A cDNA encoding a 48-kDa...
Xuan, Xuenan, Larsen, Alejandra, Ikadai, Hiromi, Tanaka, Tetsuya, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
The gene encoding the entire Babesia equi merozoite antigen 1 (EMA-1) was inserted into a baculovirus transfer vector, and a recombinant virus expressing EMA-1 was isolated. The expressed EMA-1 was...
Fukumoto, Shinya, Xuan, Xuenan, Nishikawa, Yoshifumi, Inoue, Noboru, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia gibsoni merozoite mRNA was screened with B. gibsoni-infected dog serum. cDNA encoding a 50-kDa protein was cloned and designated the P50 gene. The...
Nishikawa, Yoshifumi, Kousaka, Yuko, Tragoolpua, Khajornsak, Xuan, Xuenan, Makala, Levi, Fujisaki, Kozo, ...
The baculovirus expression system has proved to be a useful tool for the production of recombinant proteins. Here we have characterized the Neospora caninum surface protein NcSRS2 produced by two...
Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Tetsuya, Fukumoto, Shinya, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly...
Nishikawa, Yoshifumi, Tragoolpua, Khajornsak, Inoue, Noboru, Makala, Levi, Nagasawa, Hideyuki, Otsuka, Haruki, ...
Following infection with Neospora caninum, BALB/c mice were shown to be resistant to an acute infection but developed a latent chronic infection. However, BALB/c background gamma interferon...
Igarashi, Ikuo, Suzuki, Reiko, Waki, Seiji, Tagawa, Yoh-Ichi, Seng, Seyha, Tum, Sothyra, ...
Babesia microti produces a self-limiting infection in mice, and recovered mice are resistant to reinfection. In the present study, the role of T cells in protective immunity against challenge...
Avarzed, Abgaandorjiin, Igarashi, Ikuo, De Waal, Daniel T., Kawai, Satoru, Oomori, Yukio, Inoue, Noboru, ...
Monoclonal antibody (MAb) BEG3 was produced against Babesia equi parasites to define a species-specific antigen for diagnostic use. The MAb reacted with single, paired, and Maltese cross forms of B....
Huang, Xiaohong, Xuan, Xuenan, Suzuki, Hiroshi, Sugimoto, Chihiro, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A baculovirus carrying the SAG2 gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs...
Huang, Xiaohong, Xuan, Xuenan, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Hiroshi, Sugimoto, Chihiro, ...
The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both...
Fukumoto, Shinya, Xuan, Xuenan, Kadota, Kimie, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids...
Fukumoto, Shinya, Xuan, Xuenan, Takabatake, Noriyuki, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
The inhibitory effect of an antiserum to surface protein P50 of Babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells....
Ikadai, Hiromi, Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Shigeyasu, Kanemaru, Takumi, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia caballi merozoite mRNA was screened with a monoclonal antibody BC11D against the rhoptry protein of B. caballi merozoite. A cDNA encoding a 48-kDa...
Xuan, Xuenan, Larsen, Alejandra, Ikadai, Hiromi, Tanaka, Tetsuya, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
The gene encoding the entire Babesia equi merozoite antigen 1 (EMA-1) was inserted into a baculovirus transfer vector, and a recombinant virus expressing EMA-1 was isolated. The expressed EMA-1 was...
Fukumoto, Shinya, Xuan, Xuenan, Nishikawa, Yoshifumi, Inoue, Noboru, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia gibsoni merozoite mRNA was screened with B. gibsoni-infected dog serum. cDNA encoding a 50-kDa protein was cloned and designated the P50 gene. The...
Nishikawa, Yoshifumi, Kousaka, Yuko, Tragoolpua, Khajornsak, Xuan, Xuenan, Makala, Levi, Fujisaki, Kozo, ...
The baculovirus expression system has proved to be a useful tool for the production of recombinant proteins. Here we have characterized the Neospora caninum surface protein NcSRS2 produced by two...
Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Tetsuya, Fukumoto, Shinya, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly...
Nishikawa, Yoshifumi, Tragoolpua, Khajornsak, Inoue, Noboru, Makala, Levi, Nagasawa, Hideyuki, Otsuka, Haruki, ...
Following infection with Neospora caninum, BALB/c mice were shown to be resistant to an acute infection but developed a latent chronic infection. However, BALB/c background gamma interferon...
Igarashi, Ikuo, Suzuki, Reiko, Waki, Seiji, Tagawa, Yoh-Ichi, Seng, Seyha, Tum, Sothyra, ...
Babesia microti produces a self-limiting infection in mice, and recovered mice are resistant to reinfection. In the present study, the role of T cells in protective immunity against challenge...
Avarzed, Abgaandorjiin, Igarashi, Ikuo, De Waal, Daniel T., Kawai, Satoru, Oomori, Yukio, Inoue, Noboru, ...
Monoclonal antibody (MAb) BEG3 was produced against Babesia equi parasites to define a species-specific antigen for diagnostic use. The MAb reacted with single, paired, and Maltese cross forms of B....
Huang, Xiaohong, Xuan, Xuenan, Suzuki, Hiroshi, Sugimoto, Chihiro, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A baculovirus carrying the SAG2 gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs...
Huang, Xiaohong, Xuan, Xuenan, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Hiroshi, Sugimoto, Chihiro, ...
The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both...
Fukumoto, Shinya, Xuan, Xuenan, Kadota, Kimie, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids...
Fukumoto, Shinya, Xuan, Xuenan, Takabatake, Noriyuki, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
The inhibitory effect of an antiserum to surface protein P50 of Babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells....