Effect of continuous light on diurnal rhythms in Cyanothecesp. ATCC 51142 (2009)
Elvitigala, Thanura, Stöckel, Jana, Ghosh, Bijoy K, Pakrasi, Himadri B
Abstract Background Life on earth is strongly affected by alternating day and night cycles. Accordingly, many organisms have evolved an internal timekeeping system with a period of approximately 24...
Xu, Min, Bernát, Gábor, Singh, Abhay, Mi, Hualing, Rögner, Matthias, Pakrasi, Himadri B., ...
A mutant (Δ5) of Synechocystis sp. strain PCC 6803 constructed by inactivating five inorganic carbon sequestration systems did not take up CO2 or HCO3− and was unable to grow in air with or...
Xu, Min, Ogawa, Teruo, Pakrasi, Himadri B., Mi, Hualing
Antibody against cMyc cross-reacted strongly with the CupB protein tagged with His6-cMyc (HM) in thylakoid membrane of Synechocystis sp. strain PCC 6803 but only faintly with the cytoplasmic membrane...
Buchko, Garry W., Ni, Shuisong, Robinson, Howard, Welsh, Eric A., Pakrasi, Himadri B., Kennedy, Michael A.
The genome of the diurnal cyanobacterium Cyanothece sp. PCC 51142 has recently been sequenced and observed to contain 35 pentapeptide repeat proteins (PRPs). These proteins, while present throughout...
Kobayashi, Mari, Ishizuka, Tomokazu, Katayama, Mitsunori, Kanehisa, Minoru, Bhattacharyya-Pakrasi, Maitrayee, Pakrasi, Himadri B., ...
Exposure to methyl viologen in the presence of light facilitates the production of superoxide that gives severe damage on photosynthetic apparatus as well as many cellular processes in cyanobacteria...
Kashino, Yasuhiro, Koike, Hiroyuki, Yoshio, Maki, Egashira, Hirokazu, Ikeuchi, Masahiko, Pakrasi, Himadri B., ...
Using a recently introduced electrophoresis system [Kashino et al. (2001) Electrophoresis 22: 1004], components of low-molecular-mass polypeptides were analyzed in detail in photosystem II...
A Novel 3.5 kDa Protein Component of Cyanobacterial Photosystem I Complexes (1992)
Ikeuchi, Masahiko, Sonoike, Kintake, Koike, Hiroyuki, Pakrasi, Himadri B., Inoue, Yorinao
A novel protein component of 3.5 kDa was detected in photosystem I complexes prepared from several cyanobacteria, viz. Synechococcus vulcanus, Synechococcus elongotus BP-1, Synechococcus sp. FCC 7002...
McNamara, Vincent P., Sutterwala, Fayyaz S., Pakrasi, Himadri B., Whitmarsh, John
Photosystem II is a reaction center protein complex located in photosynthetic membranes of plants, algae, and cyanobacteria. Using light energy, photosystem II catalyzes the oxidation of water and...
Zak, Elena, Pakrasi, Himadri B.
Specific inhibition of photosystem I (PSI) was observed under low-temperature conditions in the cyanobacterium Synechocystis sp. strain PCC 6803. Growth at 20°C caused inhibition of PSI activity and...
Ivleva, Natalia B., Shestakov, Sergey V., Pakrasi, Himadri B.
The D1 protein is an integral component of the photosystem II reaction center complex. In the cyanobacterium Synechocystis sp. PCC 6803, D1 is synthesized with a short 16-amino acids-long...
Meetam, Metha, Keren, Nir, Ohad, Itzhak, Pakrasi, Himadri B.
A tetra-manganese cluster in the photosystem II (PSII) pigment-protein complex plays a critical role in the photosynthetic oxygen evolution process. PsbY, a small membrane-spanning polypeptide, has...
The initial steps of biogenesis of cyanobacterial photosystems occur in plasma membranes
Zak, Elena, Norling, Birgitta, Maitra, Radhashree, Huang, Fang, Andersson, Bertil, Pakrasi, Himadri B.
During oxygenic photosynthesis in cyanobacteria and chloroplasts of plants and eukaryotic algae, conversion of light energy to biologically useful chemical energy occurs in the specialized thylakoid...
Bartsevich, Victor V., Pakrasi, Himadri B.
The structure of the membrane protein MntB, a component of a manganese transporter system in Synechocystis sp. strain PCC 6803, was examined with a series of fusions to the reporter proteins alkaline...
Critical Roles of Bacterioferritins in Iron Storage and Proliferation of Cyanobacteria1[w]
Keren, Nir, Aurora, Rajeev, Pakrasi, Himadri B.
Cyanobacteria are key contributors to global photosynthetic productivity, and iron availability is essential for cyanobacterial proliferation. While iron is abundant in the earth's crust, its unique...
Thornton, Leeann E., Ohkawa, Hiroshi, Roose, Johnna L., Kashino, Yasuhiro, Keren, Nir, Pakrasi, Himadri B.
The mechanism of oxygen evolution by photosystem II (PSII) has remained highly conserved during the course of evolution from ancestral cyanobacteria to green plants. A cluster of manganese, calcium,...
Pakrasi, Himadri B., Sherman, Louis A.
A highly active O2-evolving Photosystem (PS)-II fraction has been isolated from the cyanobacterium, Anacystis nidulans R2, using an isolation buffer containing high concentrations of sucrose and...
Pakrasi, Himadri B., Goldenberg, Ann, Sherman, Louis A.
Deprivation of iron from the growth medium results in physiological as well as structural changes in the unicellular cyanobacterium Anacystis nidulans R2. Important among these changes are...
Bednarski, April E, Elgin, Sarah C.R, Pakrasi, Himadri B
This inquiry-based lab is designed around genetic diseases with a focus on protein structure and function. To allow students to work on their own investigatory projects, 10 projects on 10 different...
Keren, Nir, Ohkawa, Hiroshi, Welsh, Eric A., Liberton, Michelle, Pakrasi, Himadri B.
Photosystem II (PSII), the enzyme responsible for photosynthetic oxygen evolution, is a rapidly turned over membrane protein complex. However, the factors that regulate biogenesis of PSII are poorly...
McNamara, Vincent P., Sutterwala, Fayyaz S., Pakrasi, Himadri B., Whitmarsh, John
Photosystem II is a reaction center protein complex located in photosynthetic membranes of plants, algae, and cyanobacteria. Using light energy, photosystem II catalyzes the oxidation of water and...
Zak, Elena, Pakrasi, Himadri B.
Specific inhibition of photosystem I (PSI) was observed under low-temperature conditions in the cyanobacterium Synechocystis sp. strain PCC 6803. Growth at 20°C caused inhibition of PSI activity and...
Ivleva, Natalia B., Shestakov, Sergey V., Pakrasi, Himadri B.
The D1 protein is an integral component of the photosystem II reaction center complex. In the cyanobacterium Synechocystis sp. PCC 6803, D1 is synthesized with a short 16-amino acids-long...
Meetam, Metha, Keren, Nir, Ohad, Itzhak, Pakrasi, Himadri B.
A tetra-manganese cluster in the photosystem II (PSII) pigment-protein complex plays a critical role in the photosynthetic oxygen evolution process. PsbY, a small membrane-spanning polypeptide, has...
The initial steps of biogenesis of cyanobacterial photosystems occur in plasma membranes
Zak, Elena, Norling, Birgitta, Maitra, Radhashree, Huang, Fang, Andersson, Bertil, Pakrasi, Himadri B.
During oxygenic photosynthesis in cyanobacteria and chloroplasts of plants and eukaryotic algae, conversion of light energy to biologically useful chemical energy occurs in the specialized thylakoid...
Bartsevich, Victor V., Pakrasi, Himadri B.
The structure of the membrane protein MntB, a component of a manganese transporter system in Synechocystis sp. strain PCC 6803, was examined with a series of fusions to the reporter proteins alkaline...
Critical Roles of Bacterioferritins in Iron Storage and Proliferation of Cyanobacteria1[w]
Keren, Nir, Aurora, Rajeev, Pakrasi, Himadri B.
Cyanobacteria are key contributors to global photosynthetic productivity, and iron availability is essential for cyanobacterial proliferation. While iron is abundant in the earth's crust, its unique...
Thornton, Leeann E., Ohkawa, Hiroshi, Roose, Johnna L., Kashino, Yasuhiro, Keren, Nir, Pakrasi, Himadri B.
The mechanism of oxygen evolution by photosystem II (PSII) has remained highly conserved during the course of evolution from ancestral cyanobacteria to green plants. A cluster of manganese, calcium,...
Pakrasi, Himadri B., Sherman, Louis A.
A highly active O2-evolving Photosystem (PS)-II fraction has been isolated from the cyanobacterium, Anacystis nidulans R2, using an isolation buffer containing high concentrations of sucrose and...
Pakrasi, Himadri B., Goldenberg, Ann, Sherman, Louis A.
Deprivation of iron from the growth medium results in physiological as well as structural changes in the unicellular cyanobacterium Anacystis nidulans R2. Important among these changes are...
Bednarski, April E, Elgin, Sarah C.R, Pakrasi, Himadri B
This inquiry-based lab is designed around genetic diseases with a focus on protein structure and function. To allow students to work on their own investigatory projects, 10 projects on 10 different...
Keren, Nir, Ohkawa, Hiroshi, Welsh, Eric A., Liberton, Michelle, Pakrasi, Himadri B.
Photosystem II (PSII), the enzyme responsible for photosynthetic oxygen evolution, is a rapidly turned over membrane protein complex. However, the factors that regulate biogenesis of PSII are poorly...
Atomic structure of a nitrate-binding protein crucial for photosynthetic productivity
Koropatkin, Nicole M., Pakrasi, Himadri B., Smith, Thomas J.
Cyanobacteria, blue-green algae, are the most abundant autotrophs in aquatic environments and form the base of all aquatic food chains by fixing carbon and nitrogen into cellular biomass. The single...
The PsbQ protein defines cyanobacterial Photosystem II complexes with highest activity and stability
Roose, Johnna L., Kashino, Yasuhiro, Pakrasi, Himadri B.
Light-induced conversion of water to molecular oxygen by Photosystem II (PSII) is one of the most important enzymatic reactions in the biosphere. PSII is a multisubunit membrane protein complex with...
Buchko, Garry W., Ni, Shuisong, Robinson, Howard, Welsh, Eric A., Pakrasi, Himadri B., Kennedy, Michael A.
The genome of the diurnal cyanobacterium Cyanothece sp. PCC 51142 has recently been sequenced and observed to contain 35 pentapeptide repeat proteins (PRPs). These proteins, while present throughout...
Toepel, Jörg, Welsh, Eric, Summerfield, Tina C., Pakrasi, Himadri B., Sherman, Louis A.
We analyzed the metabolic rhythms and differential gene expression in the unicellular, diazotrophic cyanobacterium Cyanothece sp. strain ATCC 51142 under N2-fixing conditions after a shift from...
Stöckel, Jana, Welsh, Eric A., Liberton, Michelle, Kunnvakkam, Rangesh, Aurora, Rajeev, Pakrasi, Himadri B.
Cyanobacteria are photosynthetic organisms and are the only prokaryotes known to have a circadian lifestyle. Unicellular diazotrophic cyanobacteria such as Cyanothece sp. ATCC 51142 produce oxygen...
Buchko, Garry W., Robinson, Howard, Ni, Shuisong, Pakrasi, Himadri B., Kennedy, Michael A.
A construct of the Cyanothece 51142 pentapeptide-repeat protein Rfr23 (Thr27–Asp174) has been cloned, overexpressed in E. coli and purified. Diffraction data that extend to 2.5 Å and preliminary...
Welsh, Eric A., Liberton, Michelle, Stöckel, Jana, Loh, Thomas, Elvitigala, Thanura, Wang, Chunyan, ...
Unicellular cyanobacteria have recently been recognized for their contributions to nitrogen fixation in marine environments, a function previously thought to be filled mainly by filamentous...
Singh, Abhay K., Elvitigala, Thanura, Bhattacharyya-Pakrasi, Maitrayee, Aurora, Rajeev, Ghosh, Bijoy, Pakrasi, Himadri B.
Light drives the production of chemical energy and reducing equivalents in photosynthetic organisms required for the assimilation of essential nutrients. This process also generates strong oxidants...