Hiroaki Kodama

Syntheses and Chemotactic Activities of 2,3-Methanophenylalanine3Chemotactic Peptides, (9999)

Kodama, Hiroaki, Miyazaki, Masaya, Kondo, Michio, Sakaguchi, Kazuyasu, Stammer, Charles H.

A family of N-formyl Met peptides is known to have chemotactic activity for neutrophils and macrophages, and neutrophil activating potency. The SAR of these peptides have been well-documented....

Studies of Dimeric fMLF with High Chemotactic Activities, (9999)

Kondo, Michio, Miyazaki, Masaya, Fan, Jiangin, Watanabe, Teruo, Kodama, Hiroaki

A number of peptides and proteins such as C5a, fMLF (also known as fMLP) and Interleukin 8 exhibit biological activity as chemoattractants in inflammatory sites. In addition to the directed migration...

Superoxide Production in Human Neutrophils Is Enhanced by Treatment with Transmembrane Peptides Derived from Human Formyl Peptide Receptor (2006)

Hayashi, Ryo, Osada, Satoshi, Yoshiki, Masahiro, Sugiyama, Daisuke, Fujita, Ichiro, Hamasaki, Yuhei, ...

Formyl peptide receptor (FPR) mediates a number of important host defense functions. Although studies have been performed on the ligand binding site of FPR, FPR dynamic behavior such as receptor...

新規な分子機構に基づく高等植物ジーンサイレンシングの解析 (2001)

児玉, 浩明, コダマ, ヒロアキ, Kodama, Hiroaki

研究概要:リノレン酸合成を触媒するタバコ小胞体局在型w-3脂肪酸不飽和化酵素遺伝子(NtFAD3)を導入した形質転換タバコでは、ほとんどの場合、野生...

新規な分子機構に基づく高等植物ジーンサイレンシングの解析 (2001)

児玉, 浩明, コダマ, ヒロアキ, Kodama, Hiroaki

研究概要:リノレン酸合成を触媒するタバコ小胞体局在型w-3脂肪酸不飽和化酵素遺伝子(NtFAD3)を導入した形質転換タバコでは、ほとんどの場合、野生...

Side Chain Effect on Ion Channel Characters of Aib Rich Peptides (2001)

Hara, Toshiaki, Kodama, Hiroaki, Higashimoto, Yuichiro, Yamaguchi, Hiroshi, Jelokhani-Niaraki, Masood, Ehara, Tsuguhisa, ...

As models of ion channel proteins and naturally occurring pore-forming peptides, we designed a series of Aib rich peptides [Ac-(Aib-Xxx-Aib-Ala)5 NH2 (Xxx = Lys, Glu, Ser, and Gly: BXBA-20)]...

Application of Non-Perturbative Renormalization Group to Nambu-Jona-Lasinio/Gross-Neveu model at Finite Temperature and Chemical Potential (1999)

Kodama, Hiroaki, Sumi, Jun-Ichi

The chiral phase structure of the Nambu-Jona-Lasinio/Gross-Neveu model at finite temperature T and finite chemical potential \mu is investigated using (Wilsonian) Non-Perturbative Renormalization...

Structure-Function Relationship of Model Aib-Containing Peptides as Ion Transfer Intermembrane Templates (1999)

Higashimoto, Yuichiro, Kodama, Hiroaki, Jelokhani-Niaraki, Masood, Kato, Fumio, Kondo, Michio

Peptaibols comprise a family of peptide antibiotics with high contents of 2-aminoiso-butyric acid (Aib) residues and C-terminal amino alcohols. These peptides form α-helical structures leading to...

Developmental Regulation of Genes for Microsome and Plastid {omega}-3 Fatty Acid Desaturases in Wheat (Triticum aestivum L.) (1998)

Horiguchi, Gorou, Kawakami, Naoto, Kusumi, Kensuke, Kodama, Hiroaki, Iba, Koh

ω3 fatty acid desaturase is responsible for the production of α-linolenic acid, which is a major fatty acid constituent of membrane lipids in higher plants. In this study, the developmental...

Interaction and Orientation of an {alpha}-Aminoisobutyric Acid- and Tryptophan-Containing Short Helical Peptide Pore-Former in Phospholipid Vesicles, as Revealed by Fluorescence Spectroscopy (1998)

Jelokhani-Niaraki, Masood, Nakashima, Kenichi, Kodama, Hiroaki, Kondo, Michio

The interaction and orientation of a membrane protein ion channel model, an α-aminoiso-butyric acid analogue of gramicidin B (GBA), in egg yolk phosphatidylcholine vesicles was studied by means of...

Inter-meson Potentials in Dual Ginzburg-Landau Theory (1997)

Kodama, Hiroaki, Matsubara, Yoshimi, Ohno, Shigeyoshi, Suzuki, Tsuneo

We calculate inter-meson potentials numerically by solving classical equations of motion derived from Dual Ginzburg-Landau (DGL) Theory. Inter-meson potentials in DGL theory are shown to be similar...

cDNA Cloning of a Wounding-Inducible Gene Encoding a Plastid {omega}-3 Fatty Acid Desaturase from Tobacco (1996)

Hamada, Tatsurou, Nishiuchi, Takumi, Kodama, Hiroaki, Nishimura, Mitsuo, Iba, Koh

A cDNA encoding the plastid ω-3 fatty acid desaturase was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library. The amino terminal extension of the deduced amino acid sequence of...

Reversible Affinity Labeling of Opioid Receptors via Disulfide Bonding: Discriminative Labeling of {micro} and {delta} Subtypes by Chemically Activated Thiol-Containing Enkephalin Analogs (1996)

Yasunaga, Teruo, Motoyama, Shihoko, Nose, Takeru, Kodama, Hiroaki, Kondo, Michio, Shimohigashi, Yasuyuki

The 3-nitro-2-pyridinesulfenyl (Npys) group bound to a mercapto group is a highly activated electrophilic reagent, which only reacts with a free mercapto group to form a disulfide bond via the...

Dimeric Chemotactic Peptides Discriminate between Chemotaxis and Superoxide Production of Human Neutrophils (1995)

Miyazaki, Masaya, Kodama, Hiroaki, Fujita, Ichiro, Hamasaki, Yuhei, Miyazaki, Sumio, Rondo, Michio

A series of dimeric fMLPs were synthesized by cross-linking with hydrophilic linkers, mono-, di-, tri-, and tetraethylene glycols. All of the dimers showed higher activities than monomeric fMLP in...

Study on Coacervation of the Repeat Pentapeptide Model of Tropoelastin: Effect of Cations (1987)

KONDO, Michio, NAKASHIMA, Yukio, KODAMA, Hiroaki, OKAMOTO, Kouji

The effects of various cations on coacervation of the polypentapeptide (Val-Pro-Gly-Val-Gly)n a sequential model of tropoelastin, were studied by following the turbidity at 300 nm and the...

Light Actions in the Germination of Cocklebur Seeds II. Possible Origin of the Diversity of Light Responses in Seed Germination (1985)

Esashi, Esashi, Saitoh, Hisato, Saijoh, Yukio, Ishida, Satoko, Kodama, Hiroaki

In negatively photoblastic, lower seeds of cocklebur (Xanthium pennsylvanicum Wallr.), the respective germination-inhibiting effects of red (R) and far-red (FR) lights were found in the proximal and...

Light Actions in the Germination of Cocklebur Seeds: III. EFFECTS OF PRE-TREATMENT TEMPERATURE ON GERMINATION RESPONSES TO FAR-RED LIGHT AND ON DARK GERMINATION IN THE RED LIGHT-REQUIRING UPPER SEEDS (1985)

ESASHI, YOHJI, OOTA, HIROSHI, SAITOH, HISATO, KODAMA, HIROAKI

Esashi, Y., Oota, H., Saitoh, H. and Kodama, H. 1985. Light actions in the germination of cocklebur seeds. III. Effects of pre-treatment temperature on germination responses to far-red light and on...

Effect of Prostaglandin E2 on PZ-Peptidase and Several Other Peptidase Activities in a Clonal Osteoblast-Like Cell Line Derived from Newborn Mouse Calvaria (1985)

CHIKUMA, Toshiyuki, ISHII, Yoko, KATO, Takeshi, KODAMA, Hiroaki, HAKEDA, Yoshiyuki, KUMEGAWA, Masayoshi

The effects of prostaglandin E2(PGE2) on the degradation of collagen and non-collagenous peptides in clonal osteoblastic MC3T3-E1 cells were investigated by using highly sensitive assay methods for...

Light Actions in the Germination of Cocklebur Seeds: I. DIFFERENCES IN THE LIGHT RESPONSES OF THE UPPER AND LOWER SEEDS (1983)

ESASHI, YOHJI, ISHIHARA, NOBUYORI, KURAISHI, RITSU, KODAMA, HIROAKI

Germination responses to light were studied in the upper and lower seeds of cocklebur (Xanthium pennsylvanicum Wallr.). The lower seed was dark-germinating and negatively photoblastic; the upper one...

Effect of Dibutyryl Cyclic AMP on Collagen Synthesis in a Clonal Osteoblast-Like Cell Line Derived from Newborn Mouse Calvaria (1983)

HIRAMATSU, Masahiko, IKEDA, Eiko, KASHIMATA, Masanori, MINAMI, Naomi, KODAMA, Hiroaki, SUDO, Hiroko, ...

The effects of dibutyryl cyclic AMP (DBcAMP) and related compounds on collagen synthesis in a clonal osteoblast-like cell line, MC3T3-E1, were investigated. The addition of DBcAMP to cultures...

遺伝子導入に伴う転写後発現抑制機構の多面的特性に関する研究

兒玉, 浩明, コダマ, ヒロアキ, Kodama, Hiroaki, 児玉 浩明

研究種目:科学研究費補助金基盤研究(C)/報告年度:2003年度/研究課題番号:14540588/研究概要:タバコ小胞体局在型リノレン酸合成酵素遺伝子(NtFAD3)を導入...

新規な分子機構に基づく高等植物ジーンサイレンシングの解析

児玉, 浩明, コダマ, ヒロアキ, Kodama, Hiroaki

研究種目:科学研究費補助金基盤研究(C)/報告年度:2001年度/研究課題番号:12640628/研究概要:リノレン酸合成を触媒するタバコ小胞体局在型w-3脂肪酸不飽...

遺伝子導入に伴う転写後発現抑制機構の多面的特性に関する研究

児玉, 浩明, コダマ, ヒロアキ, Kodama, Hiroaki

研究種目:科学研究費補助金基盤研究(C)/報告年度:2003年度/研究課題番号:14540588/研究概要:タバコ小胞体局在型リノレン酸合成酵素遺伝子(NtFAD3)を導入...

Characterization of Transgenic Tobacco with an Increased α-Linolenic Acid Level1

Hamada, Tatsurou, Kodama, Hiroaki, Takeshita, Keizo, Utsumi, Hideo, Iba, Koh

Microsomal ω-3 fatty acid desaturase catalyzes the conversion of 18:2 (linoleic acid) to 18:3 (α-linolenic acid) in phospholipids, which are the main constituents of extrachloroplast membranes....

Wound-Induced Expression of the FAD7 Gene Is Mediated by Different Regulatory Domains of Its Promoter in Leaves/Stems and Roots1

Nishiuchi, Takumi, Kodama, Hiroaki, Yanagisawa, Shuichi, Iba, Koh

The FAD7 gene is expressed preferentially in the chlorophyllous tissues of unwounded plants. Wounding activates the expression of the FAD7 gene not only in chlorophyllous tissues, but also in...

Cardiomyocytes can be generated from marrow stromal cells in vitro

Makino, Shinji, Fukuda, Keiichi, Miyoshi, Shunichirou, Konishi, Fusako, Kodama, Hiroaki, Pan, Jing, ...

We have isolated a cardiomyogenic cell line (CMG) from murine bone marrow stromal cells. Stromal cells were immortalized, treated with 5-azacytidine, and spontaneously beating cells were repeatedly...

Phase-Specific Polypeptides and Poly(A)+ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells 1

Kodama, Hiroaki, Kawakami, Naoto, Watanabe, Akira, Komamine, Atsushi

This study shows an overall analysis of gene expression during the cell cycle in synchronous suspension cultures of Catharanthus roseus cells. First, the cellular cytoplasmic proteins were...

Isolation of Genes that Are Preferentially Expressed at the G1/S Boundary during the Cell Cycle in Synchronized Cultures of Catharanthus roseus Cells 1

Kodama, Hiroaki, Ito, Masaki, Hattori, Tsukaho, Nakamura, Kenzo, Komamine, Atsushi

A cDNA library was screened for genes that may be involved in the progression of the cell cycle of cells of higher plants. The Catharanthus roseus L. (G) Don. cells were synchronized by the double...

Polyamines and the Cell Cycle of Catharanthus roseus Cells in Culture 1

Maki, Hisae, Ando, Satoshi, Kodama, Hiroaki, Komamine, Atsushi

Investigation was made on the effect of partial depletion of polyamines (PAs), induced by treatment with inhibitors of the biosynthesis of PAs, on the distribution of cells at each phase of the cell...

Characterization of Transgenic Tobacco with an Increased α-Linolenic Acid Level1

Hamada, Tatsurou, Kodama, Hiroaki, Takeshita, Keizo, Utsumi, Hideo, Iba, Koh

Microsomal ω-3 fatty acid desaturase catalyzes the conversion of 18:2 (linoleic acid) to 18:3 (α-linolenic acid) in phospholipids, which are the main constituents of extrachloroplast membranes....

Wound-Induced Expression of the FAD7 Gene Is Mediated by Different Regulatory Domains of Its Promoter in Leaves/Stems and Roots1

Nishiuchi, Takumi, Kodama, Hiroaki, Yanagisawa, Shuichi, Iba, Koh

The FAD7 gene is expressed preferentially in the chlorophyllous tissues of unwounded plants. Wounding activates the expression of the FAD7 gene not only in chlorophyllous tissues, but also in...

Cardiomyocytes can be generated from marrow stromal cells in vitro

Makino, Shinji, Fukuda, Keiichi, Miyoshi, Shunichirou, Konishi, Fusako, Kodama, Hiroaki, Pan, Jing, ...

We have isolated a cardiomyogenic cell line (CMG) from murine bone marrow stromal cells. Stromal cells were immortalized, treated with 5-azacytidine, and spontaneously beating cells were repeatedly...

Phase-Specific Polypeptides and Poly(A)+ RNAs during the Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells 1

Kodama, Hiroaki, Kawakami, Naoto, Watanabe, Akira, Komamine, Atsushi

This study shows an overall analysis of gene expression during the cell cycle in synchronous suspension cultures of Catharanthus roseus cells. First, the cellular cytoplasmic proteins were...

Isolation of Genes that Are Preferentially Expressed at the G1/S Boundary during the Cell Cycle in Synchronized Cultures of Catharanthus roseus Cells 1

Kodama, Hiroaki, Ito, Masaki, Hattori, Tsukaho, Nakamura, Kenzo, Komamine, Atsushi

A cDNA library was screened for genes that may be involved in the progression of the cell cycle of cells of higher plants. The Catharanthus roseus L. (G) Don. cells were synchronized by the double...

Polyamines and the Cell Cycle of Catharanthus roseus Cells in Culture 1

Maki, Hisae, Ando, Satoshi, Kodama, Hiroaki, Komamine, Atsushi

Investigation was made on the effect of partial depletion of polyamines (PAs), induced by treatment with inhibitors of the biosynthesis of PAs, on the distribution of cells at each phase of the cell...

Cardiomyogenic Differentiation in Cardiac Myxoma Expressing Lineage-Specific Transcription Factors

Kodama, Hiroaki, Hirotani, Takashi, Suzuki, Yusuke, Ogawa, Satoshi, Yamazaki, Kazuto

We investigated five cases of cardiac myxoma and one case of cardiac undifferentiated sarcoma by light and electron microscopy, in situ hybridization, immunohistochemical staining, and reverse...

Vascular Endothelial Growth Factor Can Substitute for Macrophage Colony-Stimulating Factor in the Support of Osteoclastic Bone Resorption

Niida, Shumpei, Kaku, Masato, Amano, Hitoshi, Yoshida, Hisahiro, Kataoka, Hiroshi, Nishikawa, Satomi, ...

We demonstrated previously that a single injection of recombinant human macrophage colony-stimulating factor (rhM-CSF) is sufficient for osteoclast recruitment and survival in osteopetrotic (op/op)...

Preferential Proliferation of Murine Colony-forming Units in Culture in a Chemically Defined Condition with a Macrophage Colony-stimulating Factor–negative Stromal Cell Clone

Takakura, Nobuyuki, Kodama, Hiroaki, Nishikawa, Satomi, Nishikawa, Shin-Ichi

The establishment of culture conditions that selectively support hematopoietic stem cells is an important goal of hematology. In this study, we investigated the possibility of using for this purpose...

Identification of genes potentially involved in supporting hematopoietic stem cell activity of stromal cell line MC3T3-G2/PA6

Shimizu, Natsumi, Noda, Shinichi, Katayama, Kazufumi, Ichikawa, Hitoshi, Kodama, Hiroaki, Miyoshi, Hiroyuki

Although coculture of hematopoietic stem cells (HSCs) with stromal cells is a useful system to study hematopoiesis in the niche, little is known regarding the precise cellular and molecular...