Hiroaki Nakagawa

Glycoconjugate Data Bank:Structures an annotated glycan structure database and N-glycan primary structure verification service (2008)

Nakahara, Taku, Hashimoto, Ryo, Nakagawa, Hiroaki, Monde, Kenji, Miura, Nobuaki, Nishimura, Shin-Ichiro

Glycobiology has been brought to public attention as a frontier in the post-genomic era. Structural information about glycans has been accumulating in the Protein Data Bank (PDB) for years. It has...

Swainsonine reduces 5-fluorouracil tolerance in the multistage resistance of colorectal cancer cell lines (2007)

Hamaguchi, Jun, Nakagawa, Hiroaki, Takahashi, Masato, Kudo, Takeaki, Kamiyama, Naoya, Sun, Bailong, ...

Abstract Background Drug resistance is a major problem in cancer chemotherapy. Acquisition of chemo-resistance not only reduces the effectiveness of drugs, but also promotes side effects and markedly...

Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients (2007)

Nakagawa, Hiroaki, Hato, Megumi, Takegawa, Yasuhiro, Deguchi, Kisaburo, Ito, Hiroki, Takahata, Masahiko, ...

Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in α1-acid glycoprotein have...

Detection of altered N-glycan profiles in whole serum from rheumatoid arthritis patients. (2007)

Nakagawa, Hiroaki, Hato, Megumi, Takegawa, Yasuhiro, Deguchi, Kisaburo, Ito, Hiroki, Takahata, Masahiko, ...

Altered N-glycosylation occurs in many diseases. In rheumatoid arthritis (RA), for example, reduction in galactose residues in IgG and an increase in fucose residues in α1-acid glycoprotein have...

N-glycan alterations are associated with drug resistance in human hepatocellular carcinoma (2007)

Kudo, Takeaki, Nakagawa, Hiroaki, Takahashi, Masato, Hamaguchi, Jun, Kamiyama, Naoya, Yokoo, Hideki, ...

Abstract Background Correlations of disease phenotypes with glycosylation changes have been analysed intensively in the tumor biology field. Glycoforms potentially associated with carcinogenesis,...

N-Linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin (2007)

Ito, Hiroko, Akiyama, Masashi, Nakagawa, Hiroaki, Uematsu, Rie, Deguchi, Kisaburo, McMillan, James R., ...

N-Glycan oligosaccharides are thought to play multiple, important roles in a variety of biological events. However, N-glycan profiles in the stratum corneum of human skin have not yet been studied in...

N-Linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin. (2007)

Ito, Hiroko, Akiyama, Masashi, Nakagawa, Hiroaki, Uematsu, Rie, Deguchi, Kisaburo, McMillan, James R., ...

N-Glycan oligosaccharides are thought to play multiple, important roles in a variety of biological events. However, N-glycan profiles in the stratum corneum of human skin have not yet been studied in...

N-Linked neutral oligosaccharides in the stratum corneum of normal and ichthyotic skin. (2007)

Ito, Hiroko, Akiyama, Masashi, Nakagawa, Hiroaki, Uematsu, Rie, Deguchi, Kisaburo, McMillan, James R., ...

N-Glycan oligosaccharides are thought to play multiple, important roles in a variety of biological events. However, N-glycan profiles in the stratum corneum of human skin have not yet been studied in...

Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic-interaction chromatography (2006)

Takegawa, Yasuhiro, Deguchi, Kisaburo, Keira, Takuro, Ito, Hiroki, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro, ...

Isomeric oligosaccharides and isomeric glycopeptides are sometimes difficult to separate on normal-phase (NP) and reversed-phase (RP) columns. A zwitterionic type of hydrophilic-interaction...

Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic-interaction chromatography (2006)

Takegawa, Yasuhiro, Deguchi, Kisaburo, Keira, Takuro, Ito, Hiroki, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro

Isomeric oligosaccharides and isomeric glycopeptides are sometimes difficult to separate on normal-phase (NP) and reversed-phase (RP) columns. A zwitterionic type of hydrophilic-interaction...

Separation of isomeric 2-aminopyridine derivatized N-glycans and N-glycopeptides of human serum immunoglobulin G by using a zwitterionic type of hydrophilic-interaction chromatography (2006)

Takegawa, Yasuhiro, Deguchi, Kisaburo, Keira, Takuro, Ito, Hiroki, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro

Isomeric oligosaccharides and isomeric glycopeptides are sometimes difficult to separate on normal-phase (NP) and reversed-phase (RP) columns. A zwitterionic type of hydrophilic-interaction...

Structural assignment of isomeric 2-aminopyridine derivatized monosialylated biantennary N-linked oligosaccharides using negative-ion MSn spectral matching (2006)

Deguchi, Kisaburo, Takegawa, Yasuhiro, Ito, Hiroki, Miura, Nobuaki, Yoshioka, Shinji, Nagai, Shinji, ...

To investigate the possibility of structural assignment based on negative-ion MSn spectral matching, four isomers of 2-aminopyridine-derivatized (PA) monosialylated oligosaccharides (i.e., complex...

Complementary structural information of positive- and negative-ion MSn spectra of glycopeptides with neutral and sialylated N-glycans (2006)

Deguchi, Kisaburo, Ito, Hiroki, Takegawa, Yasuhiro, Shinji, Nagai, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro, ...

Positive- and negative-ion MSn spectra of chicken egg yolk glycopeptides binding a neutral and a sialylated N-glycan were acquired by using electrospray ionization linear ion trap time-of-flight mass...

Complementary structural information of positive- and negative-ion MSn spectra of glycopeptides with neutral and sialylated N-glycans (2006)

Deguchi, Kisaburo, Ito, Hiroki, Takegawa, Yasuhiro, Shinji, Nagai, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro

Positive- and negative-ion MSn spectra of chicken egg yolk glycopeptides binding a neutral and a sialylated N-glycan were acquired by using electrospray ionization linear ion trap time-of-flight mass...

Structural assignment of isomeric 2-aminopyridine derivatized monosialylated biantennary N-linked oligosaccharides using negative-ion MSn spectral matching (2006)

Deguchi, Kisaburo, Takegawa, Yasuhiro, Ito, Hiroki, Miura, Nobuaki, Yoshioka, Shinji, Nagai, Shinji, ...

To investigate the possibility of structural assignment based on negative-ion MSn spectral matching, four isomers of 2-aminopyridine-derivatized (PA) monosialylated oligosaccharides (i.e., complex...

Direct structural assignment of neutral and sialylated N-glycans of glycopeptides using collision-induced dissociation MSⁿ spectral matching. (2006)

Ito, Hiroki, Takegawa, Yasuhiro, Deguchi, Kisaburo, Nagai, Shinji, Nakagawa, Hiroaki, Shinohara, Yasuro, ...

Mass spectrometric analyses of various N-glycans binding to proteins and peptides are highly desirable for elucidating their biological roles. An approach based on collision-induced dissociation...

Direct structural assignment of neutral and sialylated N-glycans of glycopeptides using collision-induced dissociation MSⁿ spectral matching. (2006)

Ito, Hiroki, Takegawa, Yasuhiro, Deguchi, Kisaburo, Nagai, Shinji, Nakagawa, Hiroaki, Shinohara, Yasuro, ...

Mass spectrometric analyses of various N-glycans binding to proteins and peptides are highly desirable for elucidating their biological roles. An approach based on collision-induced dissociation...

Complementary structural information of positive- and negative-ion MSn spectra of glycopeptides with neutral and sialylated N-glycans (2006)

Deguchi, Kisaburo, Ito, Hiroki, Takegawa, Yasuhiro, Shinji, Nagai, Nakagawa, Hiroaki, Nishimura, Shin-Ichiro

Positive- and negative-ion MSn spectra of chicken egg yolk glycopeptides binding a neutral and a sialylated N-glycan were acquired by using electrospray ionization linear ion trap time-of-flight mass...

Structural assignment of isomeric 2-aminopyridine derivatized monosialylated biantennary N-linked oligosaccharides using negative-ion MSn spectral matching (2006)

Deguchi, Kisaburo, Takegawa, Yasuhiro, Ito, Hiroki, Miura, Nobuaki, Yoshioka, Shinji, Nagai, Shinji, ...

To investigate the possibility of structural assignment based on negative-ion MSn spectral matching, four isomers of 2-aminopyridine-derivatized (PA) monosialylated oligosaccharides (i.e., complex...

Direct structural assignment of neutral and sialylated N-glycans of glycopeptides using collision-induced dissociation MSⁿ spectral matching. (2006)

Ito, Hiroki, Takegawa, Yasuhiro, Deguchi, Kisaburo, Nagai, Shinji, Nakagawa, Hiroaki, Shinohara, Yasuro, ...

Mass spectrometric analyses of various N-glycans binding to proteins and peptides are highly desirable for elucidating their biological roles. An approach based on collision-induced dissociation...

High Throughput Quantitative Glycomics and Glycoform-focused Proteomics of Murine Dermis and Epidermis (2005)

Uematsu, Rie, Furukawa, Jun-ichi, Nakagawa, Hiroaki, Shinohara, Yasuro, Deguchi, Kisaburo, Monde, Kenji, ...

Despite recent advances in our understanding of the significance of the protein glycosylation, the throughput of protein glycosylation analysis is still too low to be applied to the exhaustive...

High Throughput Quantitative Glycomics and Glycoform-focused Proteomics of Murine Dermis and Epidermis (2005)

Uematsu, Rie, Furukawa, Jun-ichi, Nakagawa, Hiroaki, Shinohara, Yasuro, Deguchi, Kisaburo, Monde, Kenji, ...

Despite recent advances in our understanding of the significance of the protein glycosylation, the throughput of protein glycosylation analysis is still too low to be applied to the exhaustive...

High Throughput Quantitative Glycomics and Glycoform-focused Proteomics of Murine Dermis and Epidermis (2005)

Uematsu, Rie, Furukawa, Jun-ichi, Nakagawa, Hiroaki, Shinohara, Yasuro, Deguchi, Kisaburo, Monde, Kenji, ...

Despite recent advances in our understanding of the significance of the protein glycosylation, the throughput of protein glycosylation analysis is still too low to be applied to the exhaustive...

Effect of prostaglandin E1 on fractional distribution of cardiac output and organ blood flow in man: a simultaneous and non-invasive determination using double dose thallium-201 scintigraphy (1984)

ADACHI, HARUHIKO, SUGIHARA, HIROKI, NAKAGAWA, HIROAKI, OCHIAI, MASAKAZU, NAKAGAWA, MASAO, IJICHI, HAMAO

Taking advantage of the fact that the distribution of Thallium-201 in an organ almost parallels the blood flow to the organ, we devised a non-invasive method of simultaneously determining blood flow...

Essential and mutually compensatory roles of α-mannosidase II and α-mannosidase IIx in N-glycan processing in vivo in mice

Akama, Tomoya O., Nakagawa, Hiroaki, Wong, Nyet Kui, Sutton-Smith, Mark, Dell, Anne, Morris, Howard R., ...

Many proteins synthesized through the secretory pathway receive posttranslational modifications, including N-glycosylation. α-Mannosidase II (MII) is a key enzyme converting precursor...

Essential and mutually compensatory roles of α-mannosidase II and α-mannosidase IIx in N-glycan processing in vivo in mice

Akama, Tomoya O., Nakagawa, Hiroaki, Wong, Nyet Kui, Sutton-Smith, Mark, Dell, Anne, Morris, Howard R., ...

Many proteins synthesized through the secretory pathway receive posttranslational modifications, including N-glycosylation. α-Mannosidase II (MII) is a key enzyme converting precursor...

Glycoconjugate Data Bank:Structures—an annotated glycan structure database and N-glycan primary structure verification service

Nakahara, Taku, Hashimoto, Ryo, Nakagawa, Hiroaki, Monde, Kenji, Miura, Nobuaki, Nishimura, Shin-Ichiro

Glycobiology has been brought to public attention as a frontier in the post-genomic era. Structural information about glycans has been accumulating in the Protein Data Bank (PDB) for years. It has...