Multigenome DNA sequence conservation identifies Hox cis-regulatory elements (2008)
Kuntz, Steven G., Schwarz, Erich M., DeModena, John A., De Buysscher, Tristan, Trout, Diane, Shizuya, Hiroaki, ...
To learn how well ungapped sequence comparisons of multiple species can predict cis-regulatory elements in Caenorhabditis elegans, we made such predictions across the large, complex ceh-13/lin-39...
Multigenome DNA sequence conservation identifies Hox cis-regulatory elements (2008)
Kuntz, Steven G., Schwarz, Erich M., DeModena, John A., De Buysscher, Tristan, Trout, Diane, Shizuya, Hiroaki, ...
To learn how well ungapped sequence comparisons of multiple species can predict cis-regulatory elements in Caenorhabditis elegans, we made such predictions across the large, complex ceh-13/lin-39...
Kouros-Mehr, Hosein, Pintchovski, Sean, Melnyk, Jim, Chen, Yu-Jiun, Friedman, Cynthia, Trask, Barbara, ...
In mammals, the vomeronasal organ (VNO) contains chemosensory receptor cells that bind to pheromones and induce a variety of social and reproductive behaviors. It has been traditionally assumed that...
Hurowitz, Evan H., Melnyk, James M., Chen, Yu-Jiun, Kouros-Mehr, Hosein, Simon, Melvin I., Shizuya, Hiroaki
Heterotrimeric guanine nucleotide binding proteins (G proteins) transduce extracellular signals received by transmembrane receptors to effector proteins. Each subunit of the G protein complex is...
Cai, Weiwen, Jing, Junping, Irvin, Benjamin, Ohler, Lynne, Rose, Elise, Shizuya, Hiroaki, ...
Large insert clone libraries have been the primary resource used for the physical mapping of the human genome. Research directions in the genome community now are shifting direction from purely...
Kawarabayasi, Yutaka, Sawada, Mituhiro, Horikawa, Hiroshi, Haikawa, Yuji, Hino, Yumi, Yamamoto, Saori, ...
Kawarabayasi, Yutaka, Sawada, Mituhiro, Horikawa, Hiroshi, Haikawa, Yuji, Hino, Yumi, Yamamoto, Saori, ...
The complete sequence of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3, has been determined by assembling the sequences of the physical map-based contigs of fosmid...
Nielsen, Lars B., McCormick, Sally P. A., Pierotti, Vincenzo, Tam, Carmen, Gunn, Michael D., Shizuya, Hiroaki, ...
We reported previously that ~80-kilobase pair (kb) P1 bacteriophage clones spanning either the human or mouse apoB gene (clones p158 and p649, respectively) confer apoB expression in the liver of...
A bacterial artificial chromosome-based framework contig map of human chromosome 22q (1996)
Kim, Ung-Jin, Shizuya, Hiroaki, Kang, Hyung-Lyun, Choi, Sun-Shim, Garrett, Charmain L., Smink, Luc J., ...
We have constructed a physical map of human chromosome 22q using bacterial artificial chromosome (BAC) clones. The map consists of 613 chromosome 22-specific BAC clones that have been localized and...
Kim, Ung-Jin, Shizuya, Hiroaki, Deaven, Larry, Chen, Xiao-Ning, Korenberg, Julie R., Simon, Melvin I.
[No abstract]
Kim, Ung-Jin, Shizuya, Hiroaki, Deaven, Larry, Chen, Xiao-Ning, Korenberg, Julie R., Simon, Melvin I.
Stable propagation of cosmid sized human DNA inserts in an F factor based vector (1992)
Kim, Ung-Jin, Shizuya, Hiroaki, De Jong, Pieter J., Birren, Bruce, Simon, Melvin I.
Instability of complex mammalian genomic DNA inserts is commonplace in cosmid libraries constructed in conventional multicopy vectors. To develop a means to construct stable libraries, we have...
Stable propagation of cosmid sized human DNA inserts in an F factor based vector (1992)
Kim, Ung-Jin, Shizuya, Hiroaki, De Jong, Pieter J., Birren, Bruce, Simon, Melvin I.
Instability of complex mammalian genomic DNA inserts is commonplace in cosmid libraries constructed in conventional multicopy vectors. To develop a means to construct stable libraries, we have...
High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping
Cai, Weiwen, Jing, Junping, Irvin, Benjamin, Ohler, Lynne, Rose, Elise, Shizuya, Hiroaki, ...
Large insert clone libraries have been the primary resource used for the physical mapping of the human genome. Research directions in the genome community now are shifting direction from purely...
Bacterial Plasmids in Antarctic Natural Microbial Assemblages
Kobori, Hiromi, Sullivan, Cornelius W., Shizuya, Hiroaki
Samples of psychrophilic and psychrotrophic bacteria were collected from sea ice, seawater, sediments, and benthic or ice-associated animals in McMurdo Sound, Antarctica. A total of 155 strains were...
Campbell, Judith L., Shizuya, Hiroaki, Richardson, Charles C.
Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr × F− crosses, F-ductants in episome transfer, and transductants in...
Shizuya, Hiroaki, Dykhuizen, Daniel
Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain...
Korenberg, Julie R., Chen, Xiao-Ning, Sun, Zhiguang, Shi, Zheng-Yang, Ma, Shaowu, Vataru, Eddy, ...
Human genome sequencing is accelerating rapidly. Multiple genome maps link this sequence to problems in biology and clinical medicine. Because each map represents a different aspect of the structure,...
DNA Polymerase I-Dependent Mutants of Coliphage Lambda
Shizuya, Hiroaki, Brown, Douglas, Campbell, Allan
Mutagenized lysates of bacteriophage lambda were screened for mutants unable to plate on DNA polymerase I-deficient (polA−) hosts. The mutants obtained were all recombination deficient (red−)....
Synthesis of Bacteriophage Lambda DNA In Vitro: Requirement for O and P Gene Products
Shizuya, Hiroaki, Richardson, Charles C.
We have developed a cell-free system to study bacteriophage λ DNA replication. Maximal DNA synthesis in vitro requires the four deoxynucleoside triphosphates, ATP, and exogenous λ DNA. DNA...
Shizuya, Hiroaki, Livingston, Dennis M., Richardson, Charles C.
We have isolated a specialized transducing phage carrying the polC locus of E. coli K-12. A strain of E. coli lacking the lambda attachment site was infected with bacteriophage λ. Lysogens carrying...
Heat-labile alkaline phosphatase from Antarctic bacteria: Rapid 5′ end-labeling of nucleic acids
Kobori, Hiromi, Sullivan, Cornelius W., Shizuya, Hiroaki
A heat-labile alkaline phosphatase has been purified to near homogeneity from HK47, a bacterial strain isolated from Antarctic seawater. The active form of the enzyme has a molecular weight of 68,000...
High-resolution restriction maps of bacterial artificial chromosomes constructed by optical mapping
Cai, Weiwen, Jing, Junping, Irvin, Benjamin, Ohler, Lynne, Rose, Elise, Shizuya, Hiroaki, ...
Large insert clone libraries have been the primary resource used for the physical mapping of the human genome. Research directions in the genome community now are shifting direction from purely...
Bacterial Plasmids in Antarctic Natural Microbial Assemblages
Kobori, Hiromi, Sullivan, Cornelius W., Shizuya, Hiroaki
Samples of psychrophilic and psychrotrophic bacteria were collected from sea ice, seawater, sediments, and benthic or ice-associated animals in McMurdo Sound, Antarctica. A total of 155 strains were...
Campbell, Judith L., Shizuya, Hiroaki, Richardson, Charles C.
Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr × F− crosses, F-ductants in episome transfer, and transductants in...
Shizuya, Hiroaki, Dykhuizen, Daniel
Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain...
Korenberg, Julie R., Chen, Xiao-Ning, Sun, Zhiguang, Shi, Zheng-Yang, Ma, Shaowu, Vataru, Eddy, ...
Human genome sequencing is accelerating rapidly. Multiple genome maps link this sequence to problems in biology and clinical medicine. Because each map represents a different aspect of the structure,...
DNA Polymerase I-Dependent Mutants of Coliphage Lambda
Shizuya, Hiroaki, Brown, Douglas, Campbell, Allan
Mutagenized lysates of bacteriophage lambda were screened for mutants unable to plate on DNA polymerase I-deficient (polA−) hosts. The mutants obtained were all recombination deficient (red−)....
Synthesis of Bacteriophage Lambda DNA In Vitro: Requirement for O and P Gene Products
Shizuya, Hiroaki, Richardson, Charles C.
We have developed a cell-free system to study bacteriophage λ DNA replication. Maximal DNA synthesis in vitro requires the four deoxynucleoside triphosphates, ATP, and exogenous λ DNA. DNA...
Shizuya, Hiroaki, Livingston, Dennis M., Richardson, Charles C.
We have isolated a specialized transducing phage carrying the polC locus of E. coli K-12. A strain of E. coli lacking the lambda attachment site was infected with bacteriophage λ. Lysogens carrying...
Heat-labile alkaline phosphatase from Antarctic bacteria: Rapid 5′ end-labeling of nucleic acids
Kobori, Hiromi, Sullivan, Cornelius W., Shizuya, Hiroaki
A heat-labile alkaline phosphatase has been purified to near homogeneity from HK47, a bacterial strain isolated from Antarctic seawater. The active form of the enzyme has a molecular weight of 68,000...
Multigenome DNA sequence conservation identifies Hox cis-regulatory elements
Kuntz, Steven G., Schwarz, Erich M., DeModena, John A., De Buysscher, Tristan, Trout, Diane, Shizuya, Hiroaki, ...
To learn how well ungapped sequence comparisons of multiple species can predict cis-regulatory elements in Caenorhabditis elegans, we made such predictions across the large, complex ceh-13/lin-39...