Kamiya, Hiroyuki, Suzuki, Akihiro, Yamaguchi, Yuki, Handa, Hiroshi, Harashima, Hideyoshi
Oxidized RNA precursors formed in the nucleotide pool may be incorporated into RNA. In this study, the incorporation of 8-hydroxyguanosine 5'-triphosphate (8-OH-GTP; 8-oxo-7,8-dihydroguanosine...
Kuramori, Chikanori, Hase, Yasuyoshi, Hoshikawa, Koichi, Watanabe, Keiko, Nishi, Takeyuki, Hishiki, Takako, ...
Phthalate esters are commonly used plasticizers; however, some are suspected to cause reproductive toxicity. Administration of high doses of di-(2-ethylhexyl) phthalate (DEHP) induces germ cell death...
Kang, Jinsuk, Gemberling, Matthew, Nakamura, Mitsuhiro, Whitby, Frank G., Handa, Hiroshi, Fairbrother, William G., ...
Oct1 and Oct4 are homologous transcription factors with similar DNA-binding specificities. Here we show that Oct1 is dynamically phosphorylated in vivo following exposure of cells to oxidative and...
Taiko Oda, Jong-Soo Lee, Yuta Sato, Yasuaki Kabe, Satoshi Sakamoto, Hiroshi Handa, ...
N,N-Didesmethylgrossularine-1 (DDMG-1), a compound with a rare α-carboline structure, was isolated from an Indonesian ascidian Polycarpa aurata as responsible for the observed inhibitory activity...
Agawa, Yasuo, Sarhan, Moustafa, Kageyama, Yuji, Akagi, Kazutaka, Takai, Masayoshi, Hashiyama, Kazuya, ...
Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid...
Ando, Kozue, Hirao, Satoshi, Kabe, Yasuaki, Ogura, Yuji, Sato, Iwao, Yamaguchi, Yuki, ...
APE1/Ref-1 is thought to be a multifunctional protein involved in reduction–oxidation (redox) regulation and base excision DNA repair, and is required for early embryonic development in mice....
Zhu, Wenyan, Wada, Tadashi, Okabe, Sachiko, Taneda, Takuya, Yamaguchi, Yuki, Handa, Hiroshi
The transcription elongation factor 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB) sensitivity-inducing factor (DSIF) regulates RNA polymerase II (RNAPII) processivity by promoting, in concert...
Mutational Analysis of the Carboxyl-terminal Region of the SV40 Major Capsid Protein VP1 (2007)
Yokoyama, Naoki, Kawano, Masa-aki, Tsukamoto, Hiroko, Enomoto, Teruya, Inoue, Takamasa, Takahashi, Ryou-u, ...
Virus-like particles (VLPs), a promising next-generation drug delivery vehicle, can be formed in vitro using a recombinant viral capsid protein VP1 from SV40. Seventy-two VP1 pentamers interconnect...
Mutational analysis of the carboxyl-terminal region of the SV40 major capsid protein VP1 (2007)
Yokoyama, Naoki, Kawano, Masa-aki, Tsukamoto, Hiroko, Enomoto, Teruya, Inoue, Takamasa, Takahashi, Ryou-u, ...
Virus-like particles (VLPs), a promising next-generation drug delivery vehicle, can be formed in vitro using a recombinant viral capsid proteinVP1 from SV40. Seventy-two VP1 pentamers interconnect to...
Mutational analysis of the carboxyl-terminal region of the SV40 major capsid protein VP1 (2007)
Yokoyama, Naoki, Kawano, Masa-aki, Tsukamoto, Hiroko, Enomoto, Teruya, Inoue, Takamasa, Takahashi, Ryou-u, ...
Virus-like particles (VLPs), a promising next-generation drug delivery vehicle, can be formed in vitro using a recombinant viral capsid proteinVP1 from SV40. Seventy-two VP1 pentamers interconnect to...
Kubo, Tomohiro, Wada, Tadashi, Yamaguchi, Yuki, Shimizu, Akira, Handa, Hiroshi
Alternative polyadenylation leads to mRNAs with variable 3′ ends. Since a 3′-untranslated region (3′-UTR) often contains cis elements that impact stability or localization of mRNA or...
Molecular characterization of Drosophila NELF (2005)
Wu, Chwen-Huey, Lee, Chanhyo, Fan, Ruopeng, Smith, Meghan J., Yamaguchi, Yuki, Handa, Hiroshi, ...
NELF and DSIF act together to inhibit transcription elongation in vitro, and are implicated in causing promoter proximal pausing on the hsp70 gene in Drosophila. Here, further characterization of...
Azuma, Noriyuki, Tadokoro, Keiko, Asaka, Astuko, Yamada, Masao, Yamaguchi, Yuki, Handa, Hiroshi, ...
The vertebrate retina has an area where visual cells are closely packed for proper vision that is known as a fovea, an area centralis or a visual streak. The molecular mechanism that regulates the...
Azuma, Noriyuki, Tadokoro, Keiko, Asaka, Astuko, Yamada, Masao, Yamaguchi, Yuki, Handa, Hiroshi, ...
The Pax6 gene plays an important role in eye morphogenesis throughout the animal kingdom. The Pax6 gene and its homologue could form ectopic eyes by targeted expression in Drosophila and Xenopus....
Azuma, Noriyuki, Tadokoro, Keiko, Asaka, Astuko, Yamada, Masao, Yamaguchi, Yuki, Handa, Hiroshi, ...
The vertebrate retina has an area where visual cells are closely packed for proper vision that is known as a fovea, an area centralis, or a visual streak. The molecular mechanism that regulates the...
Azuma, Noriyuki, Tadokoro, Keiko, Asaka, Astuko, Yamada, Masao, Yamaguchi, Yuki, Handa, Hiroshi, ...
The Pax6 gene plays important roles in eye morphogenesis throughout the animal kingdom. The Pax6 gene and its homologue could form ectopic eyes by targeted expression in Drosophila and Xenopus. Thus,...
Aiyar, Sarah E., Sun, Jian-long, Blair, Ashley L., Moskaluk, Christopher A., Lu, Yun-zhe, Ye, Qi-nong, ...
Estrogen receptor α (ERα) signaling is paramount for normal mammary gland development and function and the repression of breast cancer. ERα function in gene regulation is mediated by a number of...
NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila (2003)
Wu, Chwen-Huey, Yamaguchi, Yuki, Benjamin, Lawrence R., Horvat-Gordon, Maria, Washinsky, Jodi, Enerly, Espen, ...
NELF and DSIF collaborate to inhibit elongation by RNA polymerase IIa in extracts from human cells. A multifaceted approach was taken to investigate the potential role of these factors in promoter...
A Rapid Purification Method for Human RNA Polymerase II by Two-Step Affinity Chromatography (2003)
Hasegawa, Jun, Endou, Masaki, Narita, Takashi, Yamada, Tomoko, Yamaguchi, Yuki, Wada, Tadashi, ...
The molecular dissection of transcription mechanisms is greatly facilitated by constructing and manipulating defined transcription systems in vitro. This approach requires highly purified...
Shimojima, Tsukasa, Okada, Masahiro, Nakayama, Takahiro, Ueda, Hitoshi, Okawa, Katsuya, Iwamatsu, Akihiro, ...
Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates...
Yamaguchi, Yuki, Narita, Takashi, Inukai, Naoto, Wada, Tadashi, Handa, Hiroshi
Suppressor of Ty (SPT) genes were originally identified through a genetic screen for mutations in the yeast Saccharomyces cerevisiae that restore gene expression disrupted by the insertion of the...
Dijk, Thamar B. Van, Baltus, Belinda, Caldenhoven, Eric, Handa, Hiroshi, Raaijmakers, J.A.M., Lammers, J.W.J., ...
High-affinity receptors for interleukin-3 (IL-3), IL-5, and granu-locyte- macrophage colony-stimulating factor (GM-CSF) are composed of two distinct subunits, a ligand-specific a chain and a common...
Wada, Tadashi, Takagi, Toshiyuki, Yamaguchi, Yuki, Ferdous, Anwarul, Imai, Takeshi, Hirose, Susumu, ...
Shimada, Hiroshi, Wada, Tadashi, Handa, Hiroshi, Ohta, Hiroyuki, Mizoguchi, Hiroshi, Nishimura, Kohji, ...
In the purple nonsulfur photosynthetic bacterium Rhodobacter sphaeroides the synthesis of components of the photosystem is regulated in response to oxygen tension and light intensity. We have...
Hirano, Fuminori, Tanaka, Hirotoshi, Makino, Yuichi, Okamoto, Kensaku, Hiramoto, Masaki, Handa, Hiroshi, ...
We studied the effects of bile acids on inducibility of the transcription factor AP-1 in human colon carcinoma LoVo cells. Firstly, cells were treated with chenodeoxycholic acid and the nuclear...
Regulation of influenza virus RNA polymerase activity by cellular and viral factors (1994)
Shimizu, Kiyoshi, Handa, Hiroshi, Nakada, Susumu, Nagata, Kyosuke
An in vitro RNA synthesis system mimicking replication of genomic influenza virus RNA was developed with nuclear extracts prepared from influenza virus-infected HeLa cells using exogenously added RNA...
Hashimoto, Toshihide, Morohashi, Ken-ichirou, Takayama, Koichi, Honda, Shin-Ichiro, Wada, Tadashi, Handa, Hiroshi, ...
We previously reported the presence of six different cis.-acting elements (Ad1 to Ad6) in the promoter region of the bovine CYP11B gene. Although the Ad1 site (TGACGTGA) was similar to a palindromic...
Kawaguchi, Haruma, Asai, Akira, Ohtsuka, Yasuji, Watanabe, Hajime, Wada, Tadashi, Handa, Hiroshi
A Simple method with the use of affinity latex particles has been developed for the fast and efficient purification of sequence-specific DNA-binding proteins on the basis of their ability to...
Akahori, Yasushi, Handa, Hiroshi, Imai, Kenji, Abe, Masumi, Kameyama, Kohzoh, Hibiya, Makoto, ...
Noncoding regions within the cluster of immunoglobulin heavy chain constant genes in the human genome contained a number of repeats. In the μ–δ intron, two repeating units were contained. One...
Purification and Characterization of Rat Low Molecular Weight Kininogen (1984)
SAKAMOTO, Wataru, YOSHIKAWA, Katsumi, UEHARA, Soichiro, NTSHTKAZE, Osamu, HANDA, Hiroshi
Low molecular weight (LMW) kininogen was isolated from pooled rat plasma by chromatography on DEAE-Sephadex A-50, CM-Sephadex C-50, Blue-Sepharose CL-6B, and Sephadex G-100. It was shown to be...
Tat-SF1 Protein Associates with RAP30 and Human SPT5 Proteins
Kim, Jae B., Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi, Sharp, Phillip A.
The potent transactivator Tat recognizes the transactivation response RNA element (TAR) of human immunodeficiency virus type 1 and stimulates the processivity of elongation of RNA polymerase (Pol) II...
Hoque, Mainul, Ishizu, Ken-ichiro, Matsumoto, Akiko, Han, Song-Iee, Arisaka, Fumio, Takayama, Makoto, ...
Adeno-associated virus capsids are composed of three proteins, VP1, VP2, and VP3. Although VP1 is necessary for viral infection, it is not essential for capsid formation. The other capsid proteins,...
Functional Interference of Sp1 and NF-κB through the Same DNA Binding Site
Hirano, Fuminori, Tanaka, Hirotoshi, Hirano, Yoshiko, Hiramoto, Masaki, Handa, Hiroshi, Makino, Isao, ...
Gene activation by NF-κB/Rel transcription factors is modulated by synergistic or antagonistic interactions with other promoter-bound transcription factors. For example, Sp1 sites are often found in...
Ishizu, Ken-Ichiro, Watanabe, Hajime, Han, Song-Iee, Kanesashi, Shin-Nosuke, Hoque, Mainul, Yajima, Hiroaki, ...
The simian virus 40 capsid is composed of 72 pentamers of VP1 protein. Although the capsid is known to dissociate to pentamers in vitro following simultaneous treatment with reducing and chelating...
Yamaguchi, Yuki, Inukai, Naoto, Narita, Takashi, Wada, Tadashi, Handa, Hiroshi
Negative elongation factor (NELF) is a human transcription factor complex that cooperates with DRB sensitivity-inducing factor (DSIF)/hSpt4-hSpt5 to repress elongation by RNA polymerase II (RNAPII)....
Narita, Takashi, Yamaguchi, Yuki, Yano, Keiichi, Sugimoto, Seiji, Chanarat, Sittinan, Wada, Tadashi, ...
The multisubunit transcription elongation factor NELF (for negative elongation factor) acts together with DRB (5,6-dichloro-1-β-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF)/human...
Li, Peggy P., Naknanishi, Akira, Tran, Mary A., Ishizu, Ken-Ichiro, Kawano, Masaaki, Phillips, Martin, ...
For polyomaviruses, calcium ions are known to be essential for virion integrity and for the assembly of capsid structures. To define the role of calcium ions in the life cycle of the virus, we...
NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila
Wu, Chwen-Huey, Yamaguchi, Yuki, Benjamin, Lawrence R., Horvat-Gordon, Maria, Washinsky, Jodi, Enerly, Espen, ...
NELF and DSIF collaborate to inhibit elongation by RNA polymerase IIa in extracts from human cells. A multifaceted approach was taken to investigate the potential role of these factors in promoter...
Shimojima, Tsukasa, Okada, Masahiro, Nakayama, Takahiro, Ueda, Hitoshi, Okawa, Katsuya, Iwamatsu, Akihiro, ...
Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates...
Expression of Early and Late Simian Virus 40 Transcripts in the Absence of Protein Synthesis
Handa, Hiroshi, Sharp, Phillip A.
We examined the synthesis of early and late simian virus 40 (SV40) mRNA's in SV40-infected cells treated with two kinds of protein synthesis inhibitors. SV40 stimulated the synthesis of mRNA's for...
Wada, Tadashi, Takagi, Toshiyuki, Yamaguchi, Yuki, Ferdous, Anwarul, Imai, Takeshi, Hirose, Susumu, ...
We report the identification of a transcription elongation factor from HeLa cell nuclear extracts that causes pausing of RNA polymerase II (Pol II) in conjunction with the transcription inhibitor...
Hartzog, Grant A., Wada, Tadashi, Handa, Hiroshi, Winston, Fred
Previous characterization of the Saccharomyces cerevisiae Spt4, Spt5, and Spt6 proteins suggested that these proteins act as transcription factors that modify chromatin structure. In this work, we...
Ainbinder, Elena, Amir-Zilberstein, Liat, Yamaguchi, Yuki, Handa, Hiroshi, Dikstein, Rivka
A20 is an immediate-early NF-κB target gene. Prior to NF-κB stimulation, the A20 promoter is bound by the polymerase II machinery to allow rapid transcription activation. Here we show that the...
Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro
Endoh, Masaki, Zhu, Wenyan, Hasegawa, Jun, Watanabe, Hajime, Kim, Dong-Ki, Aida, Masatoshi, ...
Recent studies have suggested that Spt6 participates in the regulation of transcription by RNA polymerase II (RNAPII). However, its underlying mechanism remains largely unknown. One possibility,...
Bromodomain Factor 1 (Bdf1) Is Phosphorylated by Protein Kinase CK2
Sawa, Chika, Nedea, Eduard, Krogan, Nevan, Wada, Tadashi, Handa, Hiroshi, Greenblatt, Jack, ...
Bromodomain factor 1 (Bdf1) associates with Saccharomyces cerevisiae TFIID and corresponds to the C-terminal half of higher eukaryotic TAF1. It also associates with the SWR-C complex, which is...
Mandal, Subhrangsu S., Chu, Chun, Wada, Tadashi, Handa, Hiroshi, Shatkin, Aaron J., Reinberg, Danny
Capping of the 5′ ends of nascent RNA polymerase II transcripts is the first pre-mRNA processing event in all eukaryotic cells. Capping enzyme (CE) is recruited to transcription complexes soon...
Oshima, Shigeru, Nakamura, Tetsuya, Namiki, Shin, Okada, Eriko, Tsuchiya, Kiichiro, Okamoto, Ryuichi, ...
Intestinal epithelial cell-derived interleukin (IL)-7 functions as a pleiotropic and nonredundant cytokine in the human intestinal mucosa; however, the molecular basis of its production has remained...
Aiyar, Sarah E., Sun, Jian-long, Blair, Ashley L., Moskaluk, Christopher A., Lu, Yun-zhe, Ye, Qi-nong, ...
Estrogen receptor α (ERα) signaling is paramount for normal mammary gland development and function and the repression of breast cancer. ERα function in gene regulation is mediated by a number of...
Kabe, Yasuaki, Yamada, Joe, Uga, Hitoshi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi
Osteoclast differentiation factor (ODF)/receptor activator of NF-κB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the...
Molecular characterization of Drosophila NELF
Wu, Chwen-Huey, Lee, Chanhyo, Fan, Ruopeng, Smith, Meghan J., Yamaguchi, Yuki, Handa, Hiroshi, ...
NELF and DSIF act together to inhibit transcription elongation in vitro, and are implicated in causing promoter proximal pausing on the hsp70 gene in Drosophila. Here, further characterization of...
Shimizu, Noriaki, Ouchida, Rika, Yoshikawa, Noritada, Hisada, Tetsuya, Watanabe, Hajime, Okamoto, Kensaku, ...
The HEXIM1 protein has been shown to form a protein–RNA complex composed of 7SK small nuclear RNA and positive transcription elongation factor b (P-TEFb), which is composed of cyclin-dependent...
Urano, Atsushi, Endoh, Masaki, Wada, Tadashi, Morikawa, Yoshihiro, Itoh, Miyuki, Kataoka, Yuki, ...
AF5q31 (also called MCEF) was identified by its involvement in chromosomal translocation with the gene MLL (mixed lineage leukemia), which is associated with infant acute lymphoblastic leukemia....
Aida, Masatoshi, Chen, Yexi, Nakajima, Koichi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi
Human 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II...
Tat-SF1 Protein Associates with RAP30 and Human SPT5 Proteins
Kim, Jae B., Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi, Sharp, Phillip A.
The potent transactivator Tat recognizes the transactivation response RNA element (TAR) of human immunodeficiency virus type 1 and stimulates the processivity of elongation of RNA polymerase (Pol) II...
Hoque, Mainul, Ishizu, Ken-ichiro, Matsumoto, Akiko, Han, Song-Iee, Arisaka, Fumio, Takayama, Makoto, ...
Adeno-associated virus capsids are composed of three proteins, VP1, VP2, and VP3. Although VP1 is necessary for viral infection, it is not essential for capsid formation. The other capsid proteins,...
Functional Interference of Sp1 and NF-κB through the Same DNA Binding Site
Hirano, Fuminori, Tanaka, Hirotoshi, Hirano, Yoshiko, Hiramoto, Masaki, Handa, Hiroshi, Makino, Isao, ...
Gene activation by NF-κB/Rel transcription factors is modulated by synergistic or antagonistic interactions with other promoter-bound transcription factors. For example, Sp1 sites are often found in...
Ishizu, Ken-Ichiro, Watanabe, Hajime, Han, Song-Iee, Kanesashi, Shin-Nosuke, Hoque, Mainul, Yajima, Hiroaki, ...
The simian virus 40 capsid is composed of 72 pentamers of VP1 protein. Although the capsid is known to dissociate to pentamers in vitro following simultaneous treatment with reducing and chelating...
Yamaguchi, Yuki, Inukai, Naoto, Narita, Takashi, Wada, Tadashi, Handa, Hiroshi
Negative elongation factor (NELF) is a human transcription factor complex that cooperates with DRB sensitivity-inducing factor (DSIF)/hSpt4-hSpt5 to repress elongation by RNA polymerase II (RNAPII)....
Narita, Takashi, Yamaguchi, Yuki, Yano, Keiichi, Sugimoto, Seiji, Chanarat, Sittinan, Wada, Tadashi, ...
The multisubunit transcription elongation factor NELF (for negative elongation factor) acts together with DRB (5,6-dichloro-1-β-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF)/human...
Li, Peggy P., Naknanishi, Akira, Tran, Mary A., Ishizu, Ken-Ichiro, Kawano, Masaaki, Phillips, Martin, ...
For polyomaviruses, calcium ions are known to be essential for virion integrity and for the assembly of capsid structures. To define the role of calcium ions in the life cycle of the virus, we...
NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila
Wu, Chwen-Huey, Yamaguchi, Yuki, Benjamin, Lawrence R., Horvat-Gordon, Maria, Washinsky, Jodi, Enerly, Espen, ...
NELF and DSIF collaborate to inhibit elongation by RNA polymerase IIa in extracts from human cells. A multifaceted approach was taken to investigate the potential role of these factors in promoter...
Shimojima, Tsukasa, Okada, Masahiro, Nakayama, Takahiro, Ueda, Hitoshi, Okawa, Katsuya, Iwamatsu, Akihiro, ...
Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates...
Expression of Early and Late Simian Virus 40 Transcripts in the Absence of Protein Synthesis
Handa, Hiroshi, Sharp, Phillip A.
We examined the synthesis of early and late simian virus 40 (SV40) mRNA's in SV40-infected cells treated with two kinds of protein synthesis inhibitors. SV40 stimulated the synthesis of mRNA's for...
Wada, Tadashi, Takagi, Toshiyuki, Yamaguchi, Yuki, Ferdous, Anwarul, Imai, Takeshi, Hirose, Susumu, ...
We report the identification of a transcription elongation factor from HeLa cell nuclear extracts that causes pausing of RNA polymerase II (Pol II) in conjunction with the transcription inhibitor...
Hartzog, Grant A., Wada, Tadashi, Handa, Hiroshi, Winston, Fred
Previous characterization of the Saccharomyces cerevisiae Spt4, Spt5, and Spt6 proteins suggested that these proteins act as transcription factors that modify chromatin structure. In this work, we...
Ainbinder, Elena, Amir-Zilberstein, Liat, Yamaguchi, Yuki, Handa, Hiroshi, Dikstein, Rivka
A20 is an immediate-early NF-κB target gene. Prior to NF-κB stimulation, the A20 promoter is bound by the polymerase II machinery to allow rapid transcription activation. Here we show that the...
Human Spt6 Stimulates Transcription Elongation by RNA Polymerase II In Vitro
Endoh, Masaki, Zhu, Wenyan, Hasegawa, Jun, Watanabe, Hajime, Kim, Dong-Ki, Aida, Masatoshi, ...
Recent studies have suggested that Spt6 participates in the regulation of transcription by RNA polymerase II (RNAPII). However, its underlying mechanism remains largely unknown. One possibility,...
Bromodomain Factor 1 (Bdf1) Is Phosphorylated by Protein Kinase CK2
Sawa, Chika, Nedea, Eduard, Krogan, Nevan, Wada, Tadashi, Handa, Hiroshi, Greenblatt, Jack, ...
Bromodomain factor 1 (Bdf1) associates with Saccharomyces cerevisiae TFIID and corresponds to the C-terminal half of higher eukaryotic TAF1. It also associates with the SWR-C complex, which is...
Mandal, Subhrangsu S., Chu, Chun, Wada, Tadashi, Handa, Hiroshi, Shatkin, Aaron J., Reinberg, Danny
Capping of the 5′ ends of nascent RNA polymerase II transcripts is the first pre-mRNA processing event in all eukaryotic cells. Capping enzyme (CE) is recruited to transcription complexes soon...
Oshima, Shigeru, Nakamura, Tetsuya, Namiki, Shin, Okada, Eriko, Tsuchiya, Kiichiro, Okamoto, Ryuichi, ...
Intestinal epithelial cell-derived interleukin (IL)-7 functions as a pleiotropic and nonredundant cytokine in the human intestinal mucosa; however, the molecular basis of its production has remained...
Aiyar, Sarah E., Sun, Jian-long, Blair, Ashley L., Moskaluk, Christopher A., Lu, Yun-zhe, Ye, Qi-nong, ...
Estrogen receptor α (ERα) signaling is paramount for normal mammary gland development and function and the repression of breast cancer. ERα function in gene regulation is mediated by a number of...
Kabe, Yasuaki, Yamada, Joe, Uga, Hitoshi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi
Osteoclast differentiation factor (ODF)/receptor activator of NF-κB ligand is essential for inducing the differentiation of mature osteoclasts. We find that nuclear factor Y (NF-Y) binds to the...
Molecular characterization of Drosophila NELF
Wu, Chwen-Huey, Lee, Chanhyo, Fan, Ruopeng, Smith, Meghan J., Yamaguchi, Yuki, Handa, Hiroshi, ...
NELF and DSIF act together to inhibit transcription elongation in vitro, and are implicated in causing promoter proximal pausing on the hsp70 gene in Drosophila. Here, further characterization of...
Shimizu, Noriaki, Ouchida, Rika, Yoshikawa, Noritada, Hisada, Tetsuya, Watanabe, Hajime, Okamoto, Kensaku, ...
The HEXIM1 protein has been shown to form a protein–RNA complex composed of 7SK small nuclear RNA and positive transcription elongation factor b (P-TEFb), which is composed of cyclin-dependent...
Mutations of the PAX6 Gene Detected in Patients with a Variety of Optic-Nerve Malformations
Azuma, Noriyuki, Yamaguchi, Yuki, Handa, Hiroshi, Tadokoro, Keiko, Asaka, Atsuko, Kawase, Eriko, ...
The PAX6 gene is involved in ocular morphogenesis and is expressed in the developing central nervous system and numerous ocular tissues during development. PAX6 mutations have been detected in...
Urano, Atsushi, Endoh, Masaki, Wada, Tadashi, Morikawa, Yoshihiro, Itoh, Miyuki, Kataoka, Yuki, ...
AF5q31 (also called MCEF) was identified by its involvement in chromosomal translocation with the gene MLL (mixed lineage leukemia), which is associated with infant acute lymphoblastic leukemia....
Aida, Masatoshi, Chen, Yexi, Nakajima, Koichi, Yamaguchi, Yuki, Wada, Tadashi, Handa, Hiroshi
Human 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF) and negative elongation factor (NELF) negatively regulate transcription elongation by RNA polymerase II...
Kubo, Tomohiro, Wada, Tadashi, Yamaguchi, Yuki, Shimizu, Akira, Handa, Hiroshi
Alternative polyadenylation leads to mRNAs with variable 3′ ends. Since a 3′-untranslated region (3′-UTR) often contains cis elements that impact stability or localization of mRNA or...
Adeno-Associated Virus Site-Specific Integration Is Regulated by TRP-185▿
Yamamoto, Noriaki, Suzuki, Masato, Kawano, Masa-aki, Inoue, Takamasa, Takahashi, Ryou-u, Tsukamoto, Hiroko, ...
Adeno-associated virus (AAV) integrates site specifically into the AAVS1 locus on human chromosome 19. Although recruitment of the AAV nonstructural protein Rep78/68 to the Rep binding site (RBS) on...
Zhu, Wenyan, Wada, Tadashi, Okabe, Sachiko, Taneda, Takuya, Yamaguchi, Yuki, Handa, Hiroshi
The transcription elongation factor 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB) sensitivity-inducing factor (DSIF) regulates RNA polymerase II (RNAPII) processivity by promoting, in concert...
Nakanishi, Akira, Itoh, Noriko, Li, Peggy P., Handa, Hiroshi, Liddington, Robert C., Kasamatsu, Harumi
We investigated the roles of simian virus 40 capsid proteins in the viral life cycle by analyzing point mutants in Vp1 and Vp2/3, as well as a deletion mutant lacking the Vp2/3 coding sequence. The...
Differential Regulation of NF-κB by Elongation Factors Is Determined by Core Promoter Type▿
Amir-Zilberstein, Liat, Ainbinder, Elena, Toube, Leanne, Yamaguchi, Yuki, Handa, Hiroshi, Dikstein, Rivka
NF-κB transcription factors activate genes important for immune response, inflammation, and cell survival. P-TEFb and DSIF, which are positive and negative transcription elongation factors,...
Agawa, Yasuo, Sarhan, Moustafa, Kageyama, Yuji, Akagi, Kazutaka, Takai, Masayoshi, Hashiyama, Kazuya, ...
Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-f1 is expressed in a temporally regulated manner following pulses of ecdysteroid...
Ando, Kozue, Hirao, Satoshi, Kabe, Yasuaki, Ogura, Yuji, Sato, Iwao, Yamaguchi, Yuki, ...
APE1/Ref-1 is thought to be a multifunctional protein involved in reduction–oxidation (redox) regulation and base excision DNA repair, and is required for early embryonic development in mice....
Adenine Nucleotide Translocator Transports Haem Precursors into Mitochondria
Azuma, Motoki, Kabe, Yasuaki, Kuramori, Chikanori, Kondo, Masao, Yamaguchi, Yuki, Handa, Hiroshi
Haem is a prosthetic group for haem proteins, which play an essential role in oxygen transport, respiration, signal transduction, and detoxification. In haem biosynthesis, the haem precursor...
Kang, Jinsuk, Gemberling, Matthew, Nakamura, Mitsuhiro, Whitby, Frank G., Handa, Hiroshi, Fairbrother, William G., ...
Oct1 and Oct4 are homologous transcription factors with similar DNA-binding specificities. Here we show that Oct1 is dynamically phosphorylated in vivo following exposure of cells to oxidative and...
Repression of RNA Polymerase II Elongation In Vivo Is Critically Dependent on the C-Terminus of Spt5
Chen, Hui, Contreras, Xavier, Yamaguchi, Yuki, Handa, Hiroshi, Peterlin, B. Matija, Guo, Su
The stalling of RNA polymerase II (RNAPII) at the promoters of many genes, including developmental regulators, stress-responsive genes, and HIVLTR, suggests transcription elongation as a critical...