Hiroshi Kimura

Herpes simplex virus type 2 tegument protein UL56 relocalizes ubiquitin ligase Nedd4 and has a role in transport and/or release of virions (2009)

Ushijima, Yoko, Goshima, Fumi, Kimura, Hiroshi, Nishiyama, Yukihiro

Abstract Background The ubiquitin system functions in a variety of cellular processes including protein turnover, protein sorting and trafficking. Many viruses exploit the cellular ubiquitin system...

Dust ring formation due to sublimation of dust grains drifting radially inward by the Poynting-Robertson drag: An analytical model (2009)

Kobayashi, Hiroshi, Watanabe, Sei-ichiro, Kimura, Hiroshi, Yamamoto, Tetsuo

Dust particles exposed to the stellar radiation and wind drift radially inward by the Poynting-Robertson (P-R) drag and pile up at the zone where they begin to sublime substantially. The reason they...

Downloaded from (2009)

Jun Takamatsu, Koichi Ogawara, Hiroshi Kimura, Katsushi Ikeuchi, Koichi Ogawara, Hiroshi Kimura, ...

As one of the methods for reducing the work of programming, the Learning-from-Observation (LFO) paradigm has been heavily promoted. This paradigm requires the programmer only to perform a task in...

G.: A note on the paper (2008)

Zu Guang Zhang, Hiroshi Kimura, Yasuhiro Fukuoka

Autonomously generating efficient running of a quadruped robot using delayed feedback control

Yasuhiro Fukuoka (2008)

Hiroshi Kimura, Avis H. Cohen

We have been trying to induce a quadruped robot to walk with medium walking speed on irregular terrain based on biological concepts. We propose the necessary conditions for stable dynamic walking on...

Downloaded from (2008)

Yasuhiro Fukuoka, Hiroshi Kimura, Avis H. Cohen, Yasuhiro Fukuoka, Avis H. Cohen

We have been trying to induce a quadruped robot to walk with medium walking speed on irregular terrain based on biological concepts. We propose the necessary conditions for stable dynamic walking on...

Second Order Approximation of Possible Local Displacement for Curve Objects (2008)

Hiroshi Kimura

Most methods for assembly tasks calculate possible local displacement of objects by the screw theory or its equivalence. The calculation is the first-order displacement approximation. Curvature...

Calculating Possible Local Displacement of Curve Objects using (2008)

Improved Screw Theory, Hiroshi Kimura

Various methods to recognize assembly tasks using possible local displacement of objects have been proposed. To calculate this displacement, the screw theory is employed. It is equivalent to the...

Recognition of a Mechanical Linkage (2008)

Yoshihiro Sato, Jun Takamatsu, Hiroshi Kimura, Katsushi Ikeuchi

In this paper, we propose a vision-based technique for recognition of a mechanical linkage. The aim is to realize a robot system which can autonomously operate an object with a mechanical linkage....

Nucleosome formation with the testis-specific histone H3 variant, H3t, by human nucleosome assembly proteins in vitro (2008)

Tachiwana, Hiroaki, Osakabe, Akihisa, Kimura, Hiroshi, Kurumizaka, Hitoshi

Five non-allelic histone H3 variants, H3.1, H3.2, H3.3, H3t and CENP-A, have been identified in mammals. H3t is robustly expressed in the testis, and thus was assigned as the testis-specific H3...

Alterations of Lysine Modifications on the Histone H3 N-Tail under Drought Stress Conditions in Arabidopsis thaliana (2008)

Kim, Jong-Myong, To, Taiko Kim, Ishida, Junko, Morosawa, Taeko, Kawashima, Makiko, Matsui, Akihiro, ...

Post-translational modification of histone N-tails affects eukaryotic gene activity. In Arabidopsis, the histone modification level correlates with gene activation and repression in vernalization and...

Comet 9P/Tempel 1: Interpretation with the Deep Impact Results (2007)

Yamamoto, Satoru, Kimura, Hiroshi, Zubko, Evgenij, Kobayashi, Hiroshi, Wada, Koji, Ishiguro, Masateru, ...

According to our common understandings, the original surface of a short-period comet nucleus has been lost by sublimation processes during its close approaches to the Sun. Sublimation results in the...

robot (2007)

Koichi Ogawara, Jun Takamatsu, Soshi Iba, Tomikazu Tanuki, Hiroshi Kimura, Katsushi Ikeuchi

hand-action models in task and behavior levels by a learning

呼吸不全時における肝機能障害(Ischemic Hepatopathy)について : 低酸素血症および静脈圧上昇の影響 (2007)

栗山, 喬之, 鈴木, 公典, 山岸, 文雄, 木村, 弘, 渡辺, 昌平, クリヤマ, タカユキ, ...

肝機能異常は重篤な心血管系疾患にしばしば観察され,低酸素血症や肝血流量の低下に起因する肝細胞の虚血性変化(ischemic...

Virological aspects of Epstein-Barr virus infections. (2006)

Yamashita, Nobuko, Kimura, Hiroshi, Morishima, Tsuneo

Epstein-Barr virus (EBV) is usually maintained in an asymptomatic and latent form by the host immune system, and primarily by EBV-specific cytotoxic T cells (CTLs). However, EBV has been linked to...

First two-micron imaging polarimetry of beta Pictoris (2006)

Tamura, Motohide, Fukagawa, Misato, Kimura, Hiroshi, Yamamoto, Tetsuo, Suto, Hiroshi, Abe, Lyu

High-resolution K-band imaging polarimetry of the βPic dust disk has been conducted with adaptive optics and a coronagraph using the Subaru 8.2 m telescope. Polarization of 10% is detected out to r...

First two-micron imaging polarimetry of beta Pictoris (2006)

Tamura, Motohide, Fukagawa, Misato, Kimura, Hiroshi, Yamamoto, Tetsuo, Suto, Hiroshi, Abe, Lyu

High-resolution K-band imaging polarimetry of the βPic dust disk has been conducted with adaptive optics and a coronagraph using the Subaru 8.2 m telescope. Polarization of 10% is detected out to r...

First two-micron imaging polarimetry of beta Pictoris (2006)

Tamura, Motohide, Fukagawa, Misato, Kimura, Hiroshi, Yamamoto, Tetsuo, Suto, Hiroshi, Abe, Lyu

High-resolution K-band imaging polarimetry of the βPic dust disk has been conducted with adaptive optics and a coronagraph using the Subaru 8.2 m telescope. Polarization of 10% is detected out to r...

First Two-Micron Imaging Polarimetry of Beta Pictoris (2006)

Tamura, Motohide, Fukagawa, Misato, Kimura, Hiroshi, Yamamoto, Tetsuo, Suto, Hiroshi, Abe, Lyu

High-resolution K band imaging polarimetry of the beta Pic dust disk has been conducted with adaptive optics and a coronagraph using the Subaru 8.2-m telescope. Polarization of ~10 % is detected out...

Haptoglobin Gene Promoter Polymorphism and Haplotypes Are Unique in Different Populations (2006)

Kwesi Teye, Mikiko Soejima, Hao Pang, Makoto Tsuneoka, Yoshiro Koda, ...

We investigated the distribution of haptoglobin (HP) alleles and haplotypes among Africans (Ghanaians), Europeans (from South Africa), and Chinese. HP*1F was present only in Africans and Europeans,...

Near-Infrared Photometric Monitoring of a Pre-Main-Sequence Object KH 15D (2005)

Kusakabe, Nobuhiko, Tamura, Motohide, Nakajima, Yasushi, Kandori, Ryo, Ishihara, Akika, Nagata, Tetsuya, ...

An extensive photometric monitoring of KH 15D, an enigmatic variable in the young star cluster NGC 2264, has been conducted. Simultaneous and accurate near-infrared (JHKs-bands) photometry is...

The functional organization of mitochondrial genomes in human cells (2004)

Iborra, Francisco J, Kimura, Hiroshi, Cook, Peter R

Abstract Background We analyzed the organization and function of mitochondrial DNA in a stable human cell line (ECV304, which is also known as T-24) containing mitochondria tagged with the yellow...

Dust Near The Sun (2004)

Mann,Ingrid, Kimura,Hiroshi, Biesecker,Douglas A., Tsurutani,Bruce T., Grün,Eberhard, McKibben,R. Bruce, ...

We review the current knowledge and understanding of dust in the inner solar system. The major sources of the dust population in the inner solar system are comets and asteroids, but the relative...

Improvement of Polarimetric SAR Calibration based (2004)

Kimura, Hiroshi, Mizuno, T., Papathanassiou, Konstantinos, Hajnsek, Irena

The polarimetric cross-talk calibration algorithm proposed by Quegan, which is more general than the one proposed by van Zyl, is modified to improve polarimetric SAR calibration. L-band polarimetric...

Dust Near The Sun (2004)

Mann, Ingrid, Kimura, Hiroshi, Biesecker, Douglas A., Tsurutani, Bruce T., Grün, Eberhard, McKibben, R. Bruce, ...

We review the current knowledge and understanding of dust in the inner solar system. The major sources of the dust population in the inner solar system are comets and asteroids, but the relative...

Modeling Manipulation Interactions by Hidden Markov Models (2002)

Koichi Ogawara Jun, Jun Takamatsu, Hiroshi Kimura, Katsushi Ikeuchi

This paper describes a new approach on how to teach everyday manipulation tasks to a robot under the "Learning from Observation" framework. In our previous work, to acquire low-level action...

Task Analysis Based on Observing Hands and Objects By Vision (2002)

Yoshihiro Sato Keni, Yoshihiro Sato, Keni Bernardin, Hiroshi Kimura, Katsushi Ikeuchi

In order to transmit, share and store human knowledge, it is important for a robot to be able to acquire task models and skills by observing human actions. Since vision plays an important role for...

Acquiring hand-action models by attention point analysis (2001)

Koichi Ogawara, Soshi Iba, Ý Tomikazu, Tanuki Ýý, Hiroshi Kimura, Ýýý Katsushi Ikeuchi

This paper describes our current research on learning task level representations by a robot through observation of human demonstrations. We focus on human hand actions and represent such hand actions...

Recognition of human behaviour using stereo vision and data gloves (2000)

Koichi Ogawara, Soshi Iba, Tomikazu Tanuki, Yoshihiro Sato, Akira Saegusa, Hiroshi Kimura, ...

This paper presents a novel method of constructing a human behaviour model by attention point (AP) analysis. The AP analysis consists of two steps. At the first step, it broadly observes human...

Characterization of the Human Dihydropyrimidinase-Related Protein 2 (DRP-2) Gene (1999)

Kitamura, Kenzo, Takayama, Muneyuki, Hamajima, Naoki, Nakanishi, Makoto, Sasaki, Makoto, Endo, Yuichi, ...

The genes within the dihydropyrimidinase-related protein (DRP) family were originally identified in humans by their homology to dihydropyrimidinase (DHP). Four members of this gene family, DRP-1, -2,...

Novel P/M Processing of Intermetallic Compound Using Amorphous TiAl. (1998)

Kimura, Hiroshi, Kobayashi, Shinichi

To overcome inherent fabricability problems and to obtain a fine microstructure, a novel P/M processing of titanium aluminide with amorphous TiAl powders has been developed; a dense compact was...

Consolidation of Mechanically Alloyed Amorphous CoNbZr Powder by HIP. (1998)

Kimura, Hiroshi, Myung, Wha-Nam, Kobayashi, Shinichi, Suzuki, Masayoshi, Toda, Kazutoshi

Large scale fully dense amorphous compacts of mechanically alloyed amorphous CoNbZr powder were produced using a novel HIP process of consolidation. A viscous flow mechanism was evaluated and the HIP...

Synthesis of High Hardness Intermetallic Compound by Pulse Electro-Discharge Consolidation of Amorphous TiAl Powder. (1998)

Kimura, Hiroshi, Kobayashi, Shinichi

This paper concerned metals in some important way and contained information about, utilized, or discussed these subjects: basic topics: experimental materials and material types: ti3al, tial...

Effect of Alloying Elements on the Mechanical Properties of the Stable Austenitic Stainless Steel. (1998)

Ohkubo, Naoto, Miyakusu, Katsuhisa, Uematsu, Yoshihiro, Kimura, Hiroshi

This paper concerned metals in some important way and contained information about, utilized, or discussed these subjects: basic topics: experimental materials and material types: fe-17cr-10ni-1mn,...

Uterus and endometrium: Relationship between endometrial histology, morphometry and ultrasound texture in the follicular phase of infertile women with natural menstrual cycles (1996)

Miyara, Miyoko, Sakumoto, Teturo, Takamiyagi, Naoko, Nakama, Ken, Kimura, Hiroshi, Kanazawa, Koji

The relationship between endometrial histology and ultra sonographic texture in the follicular phase was investigated. The endometrial sonographic texture of 32 infertile women with normal menstrual...

FABRICATION OF FIELD-EFFECT DEVICES BASED ON YBACUO/PRBACUO/YBACUO JUNCTIONS (1995)

Kimura, Hiroshi, Okabe, Yoichi, Kamijo, Hiroshi

In order to investigate an electric field effect in the junction, the three terminal device was fabricated. When the gate voltage of +2V (E=7x10(4) V/cm) was applied, the drain-source resistance...

Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1 (MCM3) and CDC46 proteins (1995)

Kimura, Hiroshi, Takizawa, Nanako, Nozaki, Nobuo, Sugimoto, Kazunori

Two new mouse genes encoding proteins that belong to the yeast mlnichromosome maintenance (MCM) protein family, which is involved in the initiation of ONA replication, were isolated and their...

Slippage-misalignment: to what extent does it contribute to mammalian cell mutagenesis? (1994)

Kimura, Hiroshi, Iyehara-Ogawa, Hiroaki, Kato, Takesi

Mutations in mammalian genomes are the result of several mutagenic processes that are intrinsic to cell metabolism. Analysis of the mutation spectrum of a chromosomal gene is a valuable tool for...

Specificity of mutations induced by N-methyl-N-nitrosourea in a cDNA of the hprt gene (1993)

Akagi, Toshiaki, Hiromatsu, Kohichi, IyeharaOgawa, Hiroaki, Kimura, Hiroshi, Kato, Takesi

N-Methyl-N-nitrosourea (MNU) reacts with DNA to produce a variety of lesions, of which O6-methylguanine (O6-MeG) has been implicated in the mutagenic and carcinogenic effects of this agent. The...

N-Acetoxy-N-acetyl-2-aminofluorene-induced mutatuion spectrum in a human hprt cDNA shuttle vector integrated into mammalian cells (1993)

Ogawa, Hiroaki Iyehara, Kimura, Hiroshi, Koya, Mitsuharu, Higuchi, Hirofumi, Kato, Takesi

The spectrum of mutations induced by N-acetoxy-N-acetyl-2-aminofluorene (N-AcO-AAF) was examined by the pZipHprtNeo shuttle vector in mammalian cells. The vector carries a cDNA of the human...

Mutational specificity of the carcinogen 3-amino-1-methyl-5H-pyrido[4,3-b]-indole in mammalian cells (1990)

Akagi, Toshiaki, Morota, Katsuyasu, Iyehara-Ogawa, Hiroaki, Kimura, Hiroshi, Kato, Takesi

We have used the pZipHprtNeo shuttle vector to determine the types of DNA sequence alterations induced by a potent carcinogen 3-amino-l-methyl-5H-pyrido[4,3-b]indole (Trp-P2). The shuttle vector...

Activation of Human Pancreatic Lipase Activity by Calcium and Bile Salts (1982)

KIMURA, Hiroshi, FUTAMI, Yoshitaka, TARUI, Sei-ichiro, SHINOMIYA, Takaaki

We examined the effects of calcium, bile salts and colipase, a protein cofactor, on pancreatic lipase purified from human duodenal juice. It was found that 10% gum acacia, used as a stabilizer of...

Conversion of a Plasma Group-Specific Component (Vitamin D Binding Protein) to a Macromolecule by a Protein Factor in Tissue Extracts (1982)

SHINOMIYA, Kaoru, SHINOMIYA, Takaaki, YOSHIDA, Ken-ichi, KIMURA, Hiroshi

We have studied the altered electrophoretic mobility from the α2-globulin region to the a1-globulin region of the group-specific component (Gc protein) (vitamin D binding protein) in plasma of aged...

Increase in the Susceptibility of Hemoglobin to Trypsin on Treatment with Glutathione or Cysteine (1980)

KIMURA, Hiroshi, MURATA, Hisae, UEMATSU, Heima

1. Native human hemoglobin A was digested with proteases after treatment with reduced or oxidized glutathione, cysteine, or cystine. 2. The treatment of hemoglobin with a 40-fold molar excess of...

Limited Proteolysis of p-Chloromercuribenzoate-Dissociated Hemoglobin by Trypsin (1979)

KIMURA, Hiroshi, TAKAHASHI, Noriko, MURATA, Hisae, UEMATSU, Heima

p-Chloromercuribenzoate-treated hemoglobin was digested by trypsin. The hydrolysate was subjected to gel-filtration on Bio-Gel P-4 and Sephadex G-50 columns, ion-exchange chromatography on...

Increase in the Susceptibility of Hemoglobin to Proteases upon Treatment with p-Mercuribenzoate (1978)

KIMURA, Hiroshi, YAMATO, Susumu, MURACHI, Takashi

Native human oxyhemoglobin, which has a rigid conformation resistant to proteases such as trypsin and subtilisin, could be hydrolyzed by these proteases at pH 7.0 after treatment with...

Proteolytic Degradtion of Hemoglobin-Haptoglobin Complex by Lysosomal Enzymes form Rat Liver (1975)

KIMURA, Hiroshi, TSUDZUKI, Toshihiro, MURACHI, Takashi

The catabolic degradation of hemoglobin and of its complex with haptoglobin by lysosomal enzymes from rat liver was studied with special emphasis on the action of cathepsins D and E. The digestion of...

Glycerokinase in Rat Liver: I. The Effect of Fat and Its Components on Glycerokinase Activity in Rat Liver (1973)

KIDA, Kaichi, KOBAYASHI, Katsusuke, KIMURA, Hiroshi, YUGARI, Yasumi

The effects of feeding rats with fat and its components, glycerol and fatty acids, on rat liver glycerokinase [EC 2.7.1.30] were investigated with the following results. (1) Feeding of a high fat...

Studies on Cystathionine Synthetase Characteristics of Purified Rat Liver Enzyme (1971)

KIMURA, Hiroshi, NAKAGAWA, Hachiro

Cystathionine synthetase was purified approximately 1,000 fold from rat liver by the following procedures: treatment at pH 5.5, 1st ammonium sulfate fractionation, 1st DEAE-cellulose column...

Studies on Substrate Induction of Serine Dehydratase of Rat Liver (1969)

NAKAGAWA, HACHIRO, MIURA, SHOICHI, KIMURA, HIROSHI, KANATSUNA, TAKAHIRO

Serine dehydratase [EC 4. 2. 1. 13] was induced by feeding a synthetic diet containing L-serine as the sole source of non-essential amino nitrogen (Serine-diet). Of the non-essential amino acids...

The Properties of Crystalline Serine Dehydratase of Rat Liver (1969)

NAKAGAWA, HACHIRO, KIMURA, HIROSHI

Serine dehydratase [EC 4.2.1.13] was crystallized from rat liver. Crystalline enzyme was found to be homogeneous and to have an s20, w value of 4.25 by ultracen-trifugation. Its molecular weight was...

Quantitative Analysis of Epstein-Barr Virus Load by Using a Real-Time PCR Assay

Kimura, Hiroshi, Morita, Makoto, Yabuta, Yumi, Kuzushima, Kiyotaka, Kato, Koji, Kojima, Seiji, ...

To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could...

Quantitation of RNA Polymerase II and Its Transcription Factors in an HeLa Cell: Little Soluble Holoenzyme but Significant Amounts of Polymerases Attached to the Nuclear Substructure

Kimura, Hiroshi, Tao, Yong, Roeder, Robert G., Cook, Peter R.

Various complexes that contain the core subunits of RNA polymerase II associated with different transcription factors have been isolated from eukaryotes; their precise molecular constitution depends...

Comparison of Quantitations of Viral Load in Varicella and Zoster

Kimura, Hiroshi, Kido, Shinji, Ozaki, Takao, Tanaka, Naoko, Ito, Yoshinori, Williams, Richard K., ...

The pathogenesis of varicella and zoster and the effects of antiviral treatment were investigated using real-time PCR for varicella-zoster virus (VZV) DNA in skin lesions and peripheral blood. A...

Isolation and Characterization of Mini-Tn5Km2 Insertion Mutants of Enterohemorrhagic Escherichia coli O157:H7 Deficient in Adherence to Caco-2 Cells

Tatsuno, Ichiro, Kimura, Hiroshi, Okutani, Akiko, Kanamaru, Kyoko, Abe, Hiroyuki, Nagai, Shinya, ...

Adherence of enterohemorrhagic Escherichia coli (EHEC) to intestinal epithelium is essential for initiation of the infection. To identify genes involved in adherence, an EHEC O157:H7 strain...

Pharmacokinetics and Effects of Ribavirin following Intraventricular Administration for Treatment of Subacute Sclerosing Panencephalitis

Hosoya, Mitsuaki, Mori, Shuichi, Tomoda, Akemi, Mori, Kenji, Sawaishi, Yukio, Kimura, Hiroshi, ...

Ribavirin is a broad-spectrum antiviral drug with inhibitory activity against many RNA viruses, including measles virus. Five patients with subacute sclerosing panencephalitis (SSPE) were treated...

The Functional Region of CENP-H Interacts with the Nuf2 Complex That Localizes to Centromere during Mitosis†

Mikami, Yoshikazu, Hori, Tetsuya, Kimura, Hiroshi, Fukagawa, Tatsuo

CENP-H is a constitutive centromere component that localizes to the centromere throughout the cell cycle. Because CENP-H is colocalized with CENP-A and CENP-C, it is thought to be an inner centromere...

Clonal Dissemination of Macrolide-Resistant and Penicillin-Susceptible Serotype 3 and Penicillin-Resistant Taiwan 19F-14 and 23F-15 Streptococcus pneumoniae Isolates in Japan: a Pilot Surveillance Study

Kasahara, Kei, Maeda, Koichi, Mikasa, Keiichi, Uno, Kenji, Takahashi, Ken, Konishi, Mitsuru, ...

Large-scale surveillance studies using molecular techniques such as pulsed-field gel electrophoresis (PFGE) have revealed that the spread of antibiotic-resistant pneumococci is due to clonal spread....

Herpes Simplex Virus Type 1 UL51 Protein Is Involved in Maturation and Egress of Virus Particles

Nozawa, Naoki, Kawaguchi, Yasushi, Tanaka, Michiko, Kato, Akihisa, Kato, Ai, Kimura, Hiroshi, ...

The UL51 gene of herpes simplex virus type 1 (HSV-1) encodes a phosphoprotein whose homologs are conserved throughout the herpes virus family. Recently, we reported that UL51 protein colocalizes with...

The fusion of bone-marrow-derived proinsulin-expressing cells with nerve cells underlies diabetic neuropathy

Terashima, Tomoya, Kojima, Hideto, Fujimiya, Mineko, Matsumura, Kazuhiro, Oi, Jiro, Hara, Manami, ...

Diabetic neuropathy is the most common microvascular complication of diabetes. Here we show that, in streptozotocin-induced diabetic rodents with neuropathy, a subpopulation of bone-marrow-derived...

The Constitutive Centromere Component CENP-50 Is Required for Recovery from Spindle Damage‡

Minoshima, Yukinori, Hori, Tetsuya, Okada, Masahiro, Kimura, Hiroshi, Haraguchi, Tokuko, Hiraoka, Yasushi, ...

We identified CENP-50 as a novel kinetochore component. We found that CENP-50 is a constitutive component of the centromere that colocalizes with CENP-A and CENP-H throughout the cell cycle in...

Quantitative Analysis of Epstein-Barr Virus Load by Using a Real-Time PCR Assay

Kimura, Hiroshi, Morita, Makoto, Yabuta, Yumi, Kuzushima, Kiyotaka, Kato, Koji, Kojima, Seiji, ...

To measure the virus load in patients with symptomatic Epstein-Barr virus (EBV) infections, we used a real-time PCR assay to quantify the amount of EBV DNA in blood. The real-time PCR assay could...

Quantitation of RNA Polymerase II and Its Transcription Factors in an HeLa Cell: Little Soluble Holoenzyme but Significant Amounts of Polymerases Attached to the Nuclear Substructure

Kimura, Hiroshi, Tao, Yong, Roeder, Robert G., Cook, Peter R.

Various complexes that contain the core subunits of RNA polymerase II associated with different transcription factors have been isolated from eukaryotes; their precise molecular constitution depends...

Comparison of Quantitations of Viral Load in Varicella and Zoster

Kimura, Hiroshi, Kido, Shinji, Ozaki, Takao, Tanaka, Naoko, Ito, Yoshinori, Williams, Richard K., ...

The pathogenesis of varicella and zoster and the effects of antiviral treatment were investigated using real-time PCR for varicella-zoster virus (VZV) DNA in skin lesions and peripheral blood. A...

Isolation and Characterization of Mini-Tn5Km2 Insertion Mutants of Enterohemorrhagic Escherichia coli O157:H7 Deficient in Adherence to Caco-2 Cells

Tatsuno, Ichiro, Kimura, Hiroshi, Okutani, Akiko, Kanamaru, Kyoko, Abe, Hiroyuki, Nagai, Shinya, ...

Adherence of enterohemorrhagic Escherichia coli (EHEC) to intestinal epithelium is essential for initiation of the infection. To identify genes involved in adherence, an EHEC O157:H7 strain...

Pharmacokinetics and Effects of Ribavirin following Intraventricular Administration for Treatment of Subacute Sclerosing Panencephalitis

Hosoya, Mitsuaki, Mori, Shuichi, Tomoda, Akemi, Mori, Kenji, Sawaishi, Yukio, Kimura, Hiroshi, ...

Ribavirin is a broad-spectrum antiviral drug with inhibitory activity against many RNA viruses, including measles virus. Five patients with subacute sclerosing panencephalitis (SSPE) were treated...

The Functional Region of CENP-H Interacts with the Nuf2 Complex That Localizes to Centromere during Mitosis†

Mikami, Yoshikazu, Hori, Tetsuya, Kimura, Hiroshi, Fukagawa, Tatsuo

CENP-H is a constitutive centromere component that localizes to the centromere throughout the cell cycle. Because CENP-H is colocalized with CENP-A and CENP-C, it is thought to be an inner centromere...

Clonal Dissemination of Macrolide-Resistant and Penicillin-Susceptible Serotype 3 and Penicillin-Resistant Taiwan 19F-14 and 23F-15 Streptococcus pneumoniae Isolates in Japan: a Pilot Surveillance Study

Kasahara, Kei, Maeda, Koichi, Mikasa, Keiichi, Uno, Kenji, Takahashi, Ken, Konishi, Mitsuru, ...

Large-scale surveillance studies using molecular techniques such as pulsed-field gel electrophoresis (PFGE) have revealed that the spread of antibiotic-resistant pneumococci is due to clonal spread....

Herpes Simplex Virus Type 1 UL51 Protein Is Involved in Maturation and Egress of Virus Particles

Nozawa, Naoki, Kawaguchi, Yasushi, Tanaka, Michiko, Kato, Akihisa, Kato, Ai, Kimura, Hiroshi, ...

The UL51 gene of herpes simplex virus type 1 (HSV-1) encodes a phosphoprotein whose homologs are conserved throughout the herpes virus family. Recently, we reported that UL51 protein colocalizes with...

The fusion of bone-marrow-derived proinsulin-expressing cells with nerve cells underlies diabetic neuropathy

Terashima, Tomoya, Kojima, Hideto, Fujimiya, Mineko, Matsumura, Kazuhiro, Oi, Jiro, Hara, Manami, ...

Diabetic neuropathy is the most common microvascular complication of diabetes. Here we show that, in streptozotocin-induced diabetic rodents with neuropathy, a subpopulation of bone-marrow-derived...

The Constitutive Centromere Component CENP-50 Is Required for Recovery from Spindle Damage‡

Minoshima, Yukinori, Hori, Tetsuya, Okada, Masahiro, Kimura, Hiroshi, Haraguchi, Tokuko, Hiraoka, Yasushi, ...

We identified CENP-50 as a novel kinetochore component. We found that CENP-50 is a constitutive component of the centromere that colocalizes with CENP-A and CENP-H throughout the cell cycle in...

Increased Expression of a Myc Target Gene Mina53 in Human Colon Cancer

Teye, Kwesi, Tsuneoka, Makoto, Arima, Nobuyuki, Koda, Yoshiro, Nakamura, Yasuhiro, Ueta, Yoichi, ...

Mina53 is a novel Myc target gene that we previously demonstrated to be involved in cell proliferation. We studied, here, the expression of Mina53 in colon cancer to examine its possible role in...

Indigenous Pulmonary Propionibacterium acnes Primes the Host in the Development of Sarcoid-Like Pulmonary Granulomatosis in Mice

Nishiwaki, Tetsu, Yoneyama, Hiroyuki, Eishi, Yoshinobu, Matsuo, Naoki, Tatsumi, Koichiro, Kimura, Hiroshi, ...

Although many cases of sarcoidosis are self-limiting with spontaneous remission, uncontrolled pulmonary granulomatosis with fibrosis produces intolerable long-term respiratory symptoms in a minority...

Simultaneous Quantification of Epstein-Barr Virus, Cytomegalovirus, and Human Herpesvirus 6 DNA in Samples from Transplant Recipients by Multiplex Real-Time PCR Assay▿

Wada, Kaoru, Kubota, Naomi, Ito, Yoshinori, Yagasaki, Hiroshi, Kato, Koji, Yoshikawa, Tetsushi, ...

We developed a multiplex real-time PCR assay using 6-carboxyfluorescein, 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein, and carbocyanine 5-labeled probes to simultaneously quantify...

Fusion of proinsulin-producing bone marrow-derived cells with hepatocytes in diabetes

Fujimiya, Mineko, Kojima, Hideto, Ichinose, Masumi, Arai, Ryohachi, Kimura, Hiroshi, Kashiwagi, Atsunori, ...

We previously reported that diabetes in mice is associated with the appearance of proinsulin-producing (Proins-P) cells in the liver. It was unclear, however, whether these Proins-P bone...

A novel histone exchange factor, protein phosphatase 2Cγ, mediates the exchange and dephosphorylation of H2A–H2B

Kimura, Hiroshi, Takizawa, Nanako, Allemand, Eric, Hori, Tetsuya, Iborra, Francisco J., Nozaki, Naohito, ...

In eukaryotic nuclei, DNA is wrapped around a protein octamer composed of the core histones H2A, H2B, H3, and H4, forming nucleosomes as the fundamental units of chromatin. The modification and...

Aquaporin 7 Is a β-Cell Protein and Regulator of Intraislet Glycerol Content and Glycerol Kinase Activity, β-Cell Mass, and Insulin Production and Secretion▿ †

Matsumura, Kazuhiro, Chang, Benny Hung-Junn, Fujimiya, Mineko, Chen, Weiqin, Kulkarni, Rohit N., Eguchi, Yutaka, ...

To investigate if intracellular glycerol content plays a role in the regulation of insulin secretion in pancreatic β cells, we studied the expression of the glycerol channels, or aquaglyceroporins,...

DNA Damage-Dependent Acetylation and Ubiquitination of H2AX Enhances Chromatin Dynamics▿ †

Ikura, Tsuyoshi, Tashiro, Satoshi, Kakino, Akemi, Shima, Hiroki, Jacob, Naduparambil, Amunugama, Ravindra, ...

Chromatin reorganization plays an important role in DNA repair, apoptosis, and cell cycle checkpoints. Among proteins involved in chromatin reorganization, TIP60 histone acetyltransferase has been...

MKKS Is a Centrosome-shuttling Protein Degraded by Disease-causing Mutations via CHIP-mediated Ubiquitination

Hirayama, Shoshiro, Yamazaki, Yuji, Kitamura, Akira, Oda, Yukako, Morito, Daisuke, Okawa, Katsuya, ...

McKusick–Kaufman syndrome (MKKS) is a recessively inherited human genetic disease characterized by several developmental anomalies. Mutations in the MKKS gene also cause Bardet–Biedl syndrome...

Direct Imaging of DNA in Living Cells Reveals the Dynamics of Chromosome Formation

Manders, Erik M.M., Kimura, Hiroshi, Cook, Peter R.

Individual chromosomes are not directly visible within the interphase nuclei of most somatic cells; they can only be seen during mitosis. We have developed a method that allows DNA strands to be...

Kinetics of Core Histones in Living Human Cells: Little Exchange of H3 and H4 and Some Rapid Exchange of H2b

Kimura, Hiroshi, Cook, Peter R.

Histones H2A and H2B form part of the same nucleosomal structure as H3 and H4. Stable HeLa cell lines expressing histones H2B, H3, and H4 tagged with green fluorescent protein (GFP) were established;...

The transcription cycle of RNA polymerase II in living cells

Kimura, Hiroshi, Sugaya, Kimihiko, Cook, Peter R.

RNA polymerase II transcribes most eukaryotic genes. Its catalytic subunit was tagged with green fluorescent protein and expressed in Chinese hamster cells bearing a mutation in the same subunit; it...

Nucleosome formation with the testis-specific histone H3 variant, H3t, by human nucleosome assembly proteins in vitro

Tachiwana, Hiroaki, Osakabe, Akihisa, Kimura, Hiroshi, Kurumizaka, Hitoshi

Five non-allelic histone H3 variants, H3.1, H3.2, H3.3, H3t and CENP-A, have been identified in mammals. H3t is robustly expressed in the testis, and thus was assigned as the testis-specific H3...

The UL14 Tegument Protein of Herpes Simplex Virus Type 1 Is Required for Efficient Nuclear Transport of the Alpha Transinducing Factor VP16 and Viral Capsids▿

Yamauchi, Yohei, Kiriyama, Kazuya, Kubota, Naomi, Kimura, Hiroshi, Usukura, Jiro, Nishiyama, Yukihiro

The protein encoded by the UL14 gene of herpes simplex virus type 1 (HSV-1) and HSV-2 is expressed late in infection and is a minor component of the virion tegument. An UL14-deficient HSV-1 mutant...

Entry into the electricity market: Uncertainty, competition, and mothballing options

Takashima, Ryuta, Goto, Makoto, Kimura, Hiroshi, Madarame, Haruki

The present paper analyzes the entry strategies into the electricity market of two firms that have power plants under price uncertainty and competition. We consider the symmetric and asymmetric two...

Herpes Simplex Virus Type 2 UL56 Interacts with the Ubiquitin Ligase Nedd4 and Increases Its Ubiquitination▿

Ushijima, Yoko, Koshizuka, Tetsuo, Goshima, Fumi, Kimura, Hiroshi, Nishiyama, Yukihiro

The herpes simplex virus UL56 gene is conserved among most members of the Alphaherpesvirinae family and plays a critical role in viral pathogenicity in vivo. The HSV-2 UL56 protein (UL56) is a...

Suppression of Sproutys Has a Therapeutic Effect for a Mouse Model of Ischemia by Enhancing Angiogenesis

Taniguchi, Koji, Sasaki, Ken-ichiro, Watari, Kousuke, Yasukawa, Hideo, Imaizumi, Tsutomu, Ayada, Toranoshin, ...

Sprouty proteins (Sproutys) inhibit receptor tyrosine kinase signaling and control various aspects of branching morphogenesis. In this study, we examined the physiological function of Sproutys in...

Effects of buspirone on posthypoxic ventilatory behavior in the C57BL/6J and A/J mouse strains

Yamauchi, Motoo, Dostal, Jesse, Kimura, Hiroshi, Strohl, Kingman P.

Buspirone, a partial agonist of the serotonergic 5-HT1A receptor, improves breathing irregularities in humans with Rett syndrome or brain stem injury. The purpose of this study was to examine whether...

G9a/GLP complexes independently mediate H3K9 and DNA methylation to silence transcription

Tachibana, Makoto, Matsumura, Yasuko, Fukuda, Mikiko, Kimura, Hiroshi, Shinkai, Yoichi

Methylation of DNA and lysine 9 of histone H3 (H3K9) are well-conserved epigenetic marks for transcriptional silencing. Although H3K9 methylation directs DNA methylation in filamentous fungi and...

A wave of nascent transcription on activated human genes

Wada, Youichiro, Ohta, Yoshihiro, Xu, Meng, Tsutsumi, Shuichi, Minami, Takashi, Inoue, Kenji, ...

Genome-wide studies reveal that transcription by RNA polymerase II (Pol II) is dynamically regulated. To obtain a comprehensive view of a single transcription cycle, we switched on transcription of...