Shimaoku, Motomu, Fujino, Yuji, Taenaka, Nobuyuki, Hiroi, Takachika, Kiyono, Hiroshi, Yoshiya, Ikuto
Abstract Background Recent investigations have shown that leukocyte activation is involved in the pathogenesis of ventilator-associated lung injury. This study was designed to investigate whether the...
Regulation of the IgA immune response /--by Hiroshi Kiyono. (1983)
Copy 2 is photocopy of typescript.
Tamagawa, Hiroshi, Takahashi, Ichiro, 高橋, 一郎, タカハシ, イチロウ, Takahashi, Ichirō, Furuse, Mikio, ...
Gut-associated lymphoreticular tissue (GALT), such as Peyer's patches (PP) and cecal patches, are important inductive sites for mucosal immune responses. As such, the GALT may have an epithelial...
A nontoxic mutant of cholera toxin elicits Th2-type responses for enhanced mucosal immunity
Yamamoto, Shingo, Kiyono, Hiroshi, Yamamoto, Masafumi, Imaoka, Koichi, Yamamoto, Miho, Fujihashi, Kohtaro, ...
We have characterized a nontoxic mutant of cholera toxin (CT) as a mucosal adjuvant in mice. The mutant CT was made by substitution of serine with phenylalanine at position 61 of the A subunit...
Motomu, Shimaoku, Fujino, Yuji, Taenaka, Nobuyuki, Hiroi, Takachika, Kiyono, Hiroshi, Yoshiya, Ikuto
Peyer's patches are required for oral tolerance to proteins
Fujihashi, Kohtaro, Dohi, Taeko, Rennert, Paul D., Yamamoto, Masafumi, Koga, Toshiya, Kiyono, Hiroshi, ...
To clarify the role of Peyer's patches in oral tolerance induction, BALB/c mice were treated in utero with lymphotoxin β-receptor Ig fusion protein to generate mice lacking Peyer's patches. When...
Pascual, David W., Hone, David M., Hall, Stacy, Van Ginkel, Frederik W., Yamamoto, Masafumi, Walters, Nancy, ...
Protective immunity to enterotoxigenic Escherichia coli (ETEC) is antibody (Ab) dependent; however, oral immunization with purified ETEC fimbriae fails to elicit protective immunity as a consequence...
Mucosally Induced Immunoglobulin E-Associated Inflammation in the Respiratory Tract
Simecka, Jerry W., Jackson, Raymond J., Kiyono, Hiroshi, McGhee, Jerry R.
The purpose of the present study was to determine the immunologic responses, particularly immunopathologic reactions, associated with nasal immunization with the mucosal adjuvant, cholera toxin (CT)....
Hodge, Lisa M., Marinaro, Mariarosaria, Jones, Harlan P., McGhee, Jerry R., Kiyono, Hiroshi, Simecka, Jerry W.
The purpose of the present study was to determine the extent of immunologic responses, particularly immunopathologic responses, within the upper and lower respiratory tracts after intranasal...
Nguyen, Huan H., Boyaka, Prosper N., Moldoveanu, Zina, Novak, Miroslav J., Kiyono, Hiroshi, McGhee, Jerry R., ...
We have investigated the mechanisms involved in the clearance of viral infection at the epithelium level by analyzing the activity of influenza virus-specific cytotoxic T lymphocytes (CTL) against...
Kobayashi, Masaya, Kweon, Mi-Na, Kuwata, Hirotaka, Schreiber, Robert D., Kiyono, Hiroshi, Takeda, Kiyoshi, ...
Stat3 plays an essential role in IL-10 signaling pathways. A myeloid cell-specific deletion of Stat3 resulted in inflammatory cytokine production and development of chronic enterocolitis with...
Kweon, Mi-Na, Yamamoto, Masafumi, Kajiki, Masahiro, Takahashi, Ichiro, Kiyono, Hiroshi
Systemically primed BALB/c mice developed severe diarrhea after repeated oral administration of ovalbumin (OVA). Histological analysis demonstrated that dramatic infiltration of eosinophils and mast...
Kiyono, Hiroshi, McGhee, Jerry R., Wannemuehler, Michael J., Frangakis, Maria V., Spalding, David M., Michalek, Suzanne M., ...
The first line of defense against pathogens that enter the host by the oral route appears to involve the gut-associated lymphoreticular tissue—e.g., Peyer's patches (PP). Although animals can...
Intestinal villous M cells: An antigen entry site in the mucosal epithelium
Jang, Myoung Ho, Kweon, Mi-Na, Iwatani, Koichi, Yamamoto, Masafumi, Terahara, Kazutaka, Sasakawa, Chihiro, ...
M cells located in the follicle-associated epithelium of Peyer's patches (PP) are shown to be the principal sites for the sampling of gut luminal antigens. Thus, PP have long been considered the...
Hirasawa, Masatomo, Kiyono, Hiroshi, Shiota, Tetsuo, Hull, Richard A., Curtiss, Roy, Michalek, Suzanne M., ...
Previous studies have shown that a mutant (designated C4) of Streptococcus mutans 6715 wild type (WT) is defective in glucosyltransferase (GTF)-synthesized insoluble glucan and is avirulent in...
Hirasawa, Masatoma, Kiyono, Hiroshi, Babb, James L., Shiota, Tetsuo, Michalek, Suzanne M., McGhee, Jerry R.
A mutant of Streptococcus mutans 6715 wild type (WT), designated C4, has been shown previously to be defective in glucosyltransferase synthesis of insoluble glucan and to have low virulence in...
Ohmura, Mari, Yamamoto, Masafumi, Tomiyama-Miyaji, Chikako, Yuki, Yoshikazu, Takeda, Yoshifumi, Kiyono, Hiroshi
Shiga toxin (Stx) derivatives, such as the Stx1 B subunit (StxB1), which mediates toxin binding to the membrane, and mutant Stx1 (mStx1), which is a nontoxic doubly mutated Stx1 harboring amino acid...
Takagi, Hidenori, Hiroi, Takachika, Yang, Lijun, Tada, Yoshifumi, Yuki, Yoshikazu, Takamura, Kaoru, ...
Peptide immunotherapy using multiple predominant allergen-specific T cell epitopes is a safe and promising strategy for the control of type I allergy. In this study, we developed transgenic rice...
A nontoxic mutant of cholera toxin elicits Th2-type responses for enhanced mucosal immunity
Yamamoto, Shingo, Kiyono, Hiroshi, Yamamoto, Masafumi, Imaoka, Koichi, Yamamoto, Miho, Fujihashi, Kohtaro, ...
We have characterized a nontoxic mutant of cholera toxin (CT) as a mucosal adjuvant in mice. The mutant CT was made by substitution of serine with phenylalanine at position 61 of the A subunit...
Motomu, Shimaoku, Fujino, Yuji, Taenaka, Nobuyuki, Hiroi, Takachika, Kiyono, Hiroshi, Yoshiya, Ikuto
Peyer's patches are required for oral tolerance to proteins
Fujihashi, Kohtaro, Dohi, Taeko, Rennert, Paul D., Yamamoto, Masafumi, Koga, Toshiya, Kiyono, Hiroshi, ...
To clarify the role of Peyer's patches in oral tolerance induction, BALB/c mice were treated in utero with lymphotoxin β-receptor Ig fusion protein to generate mice lacking Peyer's patches. When...
Pascual, David W., Hone, David M., Hall, Stacy, Van Ginkel, Frederik W., Yamamoto, Masafumi, Walters, Nancy, ...
Protective immunity to enterotoxigenic Escherichia coli (ETEC) is antibody (Ab) dependent; however, oral immunization with purified ETEC fimbriae fails to elicit protective immunity as a consequence...
Mucosally Induced Immunoglobulin E-Associated Inflammation in the Respiratory Tract
Simecka, Jerry W., Jackson, Raymond J., Kiyono, Hiroshi, McGhee, Jerry R.
The purpose of the present study was to determine the immunologic responses, particularly immunopathologic reactions, associated with nasal immunization with the mucosal adjuvant, cholera toxin (CT)....
Hodge, Lisa M., Marinaro, Mariarosaria, Jones, Harlan P., McGhee, Jerry R., Kiyono, Hiroshi, Simecka, Jerry W.
The purpose of the present study was to determine the extent of immunologic responses, particularly immunopathologic responses, within the upper and lower respiratory tracts after intranasal...
Nguyen, Huan H., Boyaka, Prosper N., Moldoveanu, Zina, Novak, Miroslav J., Kiyono, Hiroshi, McGhee, Jerry R., ...
We have investigated the mechanisms involved in the clearance of viral infection at the epithelium level by analyzing the activity of influenza virus-specific cytotoxic T lymphocytes (CTL) against...
Kobayashi, Masaya, Kweon, Mi-Na, Kuwata, Hirotaka, Schreiber, Robert D., Kiyono, Hiroshi, Takeda, Kiyoshi, ...
Stat3 plays an essential role in IL-10 signaling pathways. A myeloid cell-specific deletion of Stat3 resulted in inflammatory cytokine production and development of chronic enterocolitis with...
Kweon, Mi-Na, Yamamoto, Masafumi, Kajiki, Masahiro, Takahashi, Ichiro, Kiyono, Hiroshi
Systemically primed BALB/c mice developed severe diarrhea after repeated oral administration of ovalbumin (OVA). Histological analysis demonstrated that dramatic infiltration of eosinophils and mast...
Kiyono, Hiroshi, McGhee, Jerry R., Wannemuehler, Michael J., Frangakis, Maria V., Spalding, David M., Michalek, Suzanne M., ...
The first line of defense against pathogens that enter the host by the oral route appears to involve the gut-associated lymphoreticular tissue—e.g., Peyer's patches (PP). Although animals can...
Intestinal villous M cells: An antigen entry site in the mucosal epithelium
Jang, Myoung Ho, Kweon, Mi-Na, Iwatani, Koichi, Yamamoto, Masafumi, Terahara, Kazutaka, Sasakawa, Chihiro, ...
M cells located in the follicle-associated epithelium of Peyer's patches (PP) are shown to be the principal sites for the sampling of gut luminal antigens. Thus, PP have long been considered the...
Hirasawa, Masatomo, Kiyono, Hiroshi, Shiota, Tetsuo, Hull, Richard A., Curtiss, Roy, Michalek, Suzanne M., ...
Previous studies have shown that a mutant (designated C4) of Streptococcus mutans 6715 wild type (WT) is defective in glucosyltransferase (GTF)-synthesized insoluble glucan and is avirulent in...
Hirasawa, Masatoma, Kiyono, Hiroshi, Babb, James L., Shiota, Tetsuo, Michalek, Suzanne M., McGhee, Jerry R.
A mutant of Streptococcus mutans 6715 wild type (WT), designated C4, has been shown previously to be defective in glucosyltransferase synthesis of insoluble glucan and to have low virulence in...
Ohmura, Mari, Yamamoto, Masafumi, Tomiyama-Miyaji, Chikako, Yuki, Yoshikazu, Takeda, Yoshifumi, Kiyono, Hiroshi
Shiga toxin (Stx) derivatives, such as the Stx1 B subunit (StxB1), which mediates toxin binding to the membrane, and mutant Stx1 (mStx1), which is a nontoxic doubly mutated Stx1 harboring amino acid...
Takagi, Hidenori, Hiroi, Takachika, Yang, Lijun, Tada, Yoshifumi, Yuki, Yoshikazu, Takamura, Kaoru, ...
Peptide immunotherapy using multiple predominant allergen-specific T cell epitopes is a safe and promising strategy for the control of type I allergy. In this study, we developed transgenic rice...
Tamagawa, Hiroshi, Takahashi, Ichiro, 高橋, 一郎, タカハシ, イチロウ, Takahashi, Ichirō, Furuse, Mikio, ...
Gut-associated lymphoreticular tissue (GALT), such as Peyer's patches (PP) and cecal patches, are important inductive sites for mucosal immune responses. As such, the GALT may have an epithelial...
Pathological Role of Large Intestinal IL-12p40 for the Induction of Th2-Type Allergic Diarrhea
Hino, Ayako, Kweon, Mi-Na, Fujihashi, Kohtaro, McGhee, Jerry R., Kiyono, Hiroshi
IL-12 consists of two disulfide-linked subunits, p40 and p35, that form functionally active heterodimers for the induction of Th1 cells. In contrast to IL-12 heterodimers, p40 monomers and homodimers...
Boyaka, Prosper N., Wright, Peter F., Marinaro, Mariarosaria, Kiyono, Hiroshi, Johnson, Joyce E., Gonzales, Ricardo A., ...
Subepithelial and intraepithelial lymphocytes of human adenoids and tonsils were characterized and directly compared to determine the potential contribution of these tissues to mucosal and systemic...
Okuda, Yoshiko, Takahashi, Ichiro, Kim, Jin-Kyung, Ohta, Noriyuki, Iwatani, Kouichi, Iijima, Hideki, ...
Forbidden CD4+ββ T cells, which produce interleukin (IL)-4 predominantly, are a pathological subset in the development of colitis in T-cell receptor α chain (TCRα)-deficient mice. Stimulation of...
Role of Peyer's patches in the induction of Helicobacter pylori-induced gastritis
Nagai, Shigenori, Mimuro, Hitomi, Yamada, Taketo, Baba, Yukiko, Moro, Kazuyo, Nochi, Tomonori, ...
Helicobacter pylori is a Gram-negative spiral bacterium that causes gastritis and peptic ulcer and has been implicated in the pathogenesis of gastric adenocarcinoma and mucosa-associated lymphoid...
Ohka, Seii, Igarashi, Hiroko, Nagata, Noriyo, Sakai, Mai, Koike, Satoshi, Nochi, Tomonori, ...
Poliovirus (PV) is easily transferred to humans orally; however, no rodent model for oral infections has been developed because of the alimentary tract's low sensitivity to the virus. Here we showed...
Rice-based mucosal vaccine as a global strategy for cold-chain- and needle-free vaccination
Nochi, Tomonori, Takagi, Hidenori, Yuki, Yoshikazu, Yang, Lijun, Masumura, Takehiro, Mejima, Mio, ...
Capable of inducing antigen-specific immune responses in both systemic and mucosal compartments without the use of syringe and needle, mucosal vaccination is considered ideal for the global control...
Sphingosine 1-phosphate dependence in the regulation of lymphocyte trafficking to the gut epithelium
Kunisawa, Jun, Kurashima, Yosuke, Higuchi, Morio, Gohda, Masashi, Ishikawa, Izumi, Ogahara, Ikuko, ...
It is well established that intraepithelial T lymphocytes (IELs) are derived from conventional single-positive (SP) thymocytes, as well as unconventional double-negative (DN) thymocytes and...
Dohi, Taeko, Fujihashi, Kohtaro, Rennert, Paul D., Iwatani, Koichi, Kiyono, Hiroshi, McGhee, Jerry R.
To investigate the potential involvement of T helper (Th)2-type responses in murine models of intestinal inflammation, we used trinitrobenzene sulfonic acid (TNBS)–hapten to induce inflammatory...
Iijima, Hideki, Takahashi, Ichiro, Kishi, Daisuke, Kim, Jin-Kyung, Kawano, Sunao, Hori, Masatsugu, ...
T cell receptor α chain–deficient (TCR-α−/−) mice are known to spontaneously develop inflammatory bowel disease (IBD). The colitis that develops in these mice is associated with increased...
Marinaro, Mariarosaria, Boyaka, Prosper N., Finkelman, Fred D., Kiyono, Hiroshi, Jackson, Raymond J., Jirillo, Emilio, ...
Our past studies have shown that the mucosal adjuvant cholera toxin (CT) induces T helper type 2 (Th2) responses with systemic IgG1, IgE and mucosal secretory IgA (S-IgA) antibodies (Abs). In this...
Yamamoto, Shingo, Takeda, Yoshifumi, Yamamoto, Masafumi, Kurazono, Hisao, Imaoka, Koichi, Yamamoto, Miho, ...
Cholera toxin (CT), the most commonly used mucosal adjuvant in experimental animals, is unsuitable for humans because of potent diarrhea-inducing properties. We have constructed two CT-A subunit...
Nochi, Tomonori, Yuki, Yoshikazu, Matsumura, Akiko, Mejima, Mio, Terahara, Kazutaka, Kim, Dong-Young, ...
Mucosally ingested and inhaled antigens are taken up by membranous or microfold cells (M cells) in the follicle-associated epithelium of Peyer's patches or nasopharynx-associated lymphoid tissue. We...
Peyer's Patches Are Required for Intestinal Immunoglobulin A Responses to Salmonella spp.▿
Hashizume, Tomomi, Togawa, Atsushi, Nochi, Tomonori, Igarashi, Osamu, Kweon, Mi-Na, Kiyono, Hiroshi, ...
Previous studies have shown that Peyer's patches (PP) are not required for intestinal immunoglobulin A (IgA) responses to orally administered soluble protein. However, the roles of PP in regulation...
Momoi, Fumiki, Hashizume, Tomomi, Kurita-Ochiai, Tomoko, Yuki, Yoshikazu, Kiyono, Hiroshi, Yamamoto, Masafumi
In this study, we demonstrated that the 40-kDa outer membrane protein of Porphyromonas gingivalis (40-kDa OMP) nasally administered with a nontoxic chimeric adjuvant that combines the A subunit of...