Families of classical porins...............................................................................................................................595
Families of classical porins...............................................................................................................................595
Sequence of the malK gene in E.coli K12 (1982)
Gilson, Eric, Nikaido, Hiroshi, Hofnung, Maurice
We present the sequence of gene malK which encodes a component of the system for maltose transport in E.coli K12. We also determined the position of deletion (S50) which fuses malK to the following...
X-Ray Diffraction Studies of Outer Membranes of Salmonella typhimurium (1979)
UEKI, Tatzuo, MITSUI, Toshio, NIKAIDO, Hiroshi
X-ray diffraction studies were carried out on the outer membranes of various strains of Salmonella typhimurium. Ten distinct diffraction peaks which seem to be caused by protein assemblies were...
Isolation of Uridine Diphosphogalactose from Escherichia coli Mutant Cells (1964)
MORIKAWA, NOBUKO, IMAE, YASUO, NIKAIDO, HIROSHI
An improved method for the isolation of UDPgalactose from a mutant of Escherichia coli is described. The purity of the preparation is about 95% when it is measured enzymatically and...
Bypassing the periplasm: Reconstitution of the AcrAB multidrug efflux pump of Escherichia coli
Zgurskaya, Helen I., Nikaido, Hiroshi
AcrAB is a constitutively expressed, major multidrug efflux system of Escherichia coli. We have purified the cytoplasmic membrane component, AcrB, to near homogeneity, and reconstituted the protein...
Mycolic acids are a major constituent of the mycobacterial cell wall, and they form an effective permeability barrier to protect mycobacteria from antimicrobial agents. Although the chemical...
Aires, Julio Ramos, Köhler, Thilo, Nikaido, Hiroshi, Plésiat, Patrick
A mutant, named 11B, hypersusceptible to aminoglycosides, tetracycline, and erythromycin was isolated after Tn501 insertion mutagenesis of Pseudomonas aeruginosa PAO1. Cloning and sequencing...
Mazzariol, Annarita, Cornaglia, Giuseppe, Nikaido, Hiroshi
The roles of the AmpC chromosomal β-lactamase and the AcrAB efflux system in levels of intrinsic resistance and susceptibility of Escherichia coli to β-lactams were studied with a set of isogenic...
Mazzariol, Annarita, Tokue, Yutaka, Kanegawa, Tiffany M., Cornaglia, Giuseppe, Nikaido, Hiroshi
Immunoblotting with antibody against AcrA, an obligatory component of the AcrAB multidrug efflux system, showed that this protein was overexpressed by ≥170% in 9 of 10 clinical isolates of...
Raimondi, Alessandro, Sisto, Francesca, Nikaido, Hiroshi
Starting from a clinical isolate of Serratia marcescens that produced a chromosomally encoded AmpC β-lactamase inducibly, we isolated by stepwise selection two laboratory mutants that showed high...
Miyamae, Shin, Ueda, Ohmi, Yoshimura, Fuminobu, Hwang, Jaiweon, Tanaka, Yoshinobu, Nikaido, Hiroshi
We cloned a gene, bexA, that codes for a multidrug efflux transporter from the chromosomal DNA of Bacteroides thetaiotaomicron ATCC 29741 by using an Escherichia coli ΔacrAB ΔacrEF mutant as a...
Zhang, Ying, Scorpio, Angelo, Nikaido, Hiroshi, Sun, Zhonghe
Pyrazinamide (PZA) is an important antituberculosis drug. Unlike most antibacterial agents, PZA, despite its remarkable in vivo activity, has no activity against Mycobacterium tuberculosis in vitro...
AcrD of Escherichia coli Is an Aminoglycoside Efflux Pump
Rosenberg, Emiko Y., Ma, Dzwokai, Nikaido, Hiroshi
AcrD, a transporter belonging to the resistance-nodulation-division family, was shown to participate in the efflux of aminoglycosides. Deletion of the acrD gene decreased the MICs of amikacin,...
Zgurskaya, Helen I., Nikaido, Hiroshi
In Escherichia coli, the intrinsic levels of resistance to multiple antimicrobial agents are produced through expression of the three-component multidrug efflux system AcrAB-TolC. AcrB is a...
Active Efflux of Norfloxacin by Bacteroides fragilis
Miyamae, Shin, Nikaido, Hiroshi, Tanaka, Yoshinobu, Yoshimura, Fuminobu
Norfloxacin was actively pumped out by Bacteroides fragilis, which is intrinsically resistant to most fluoroquinolones. Reserpine moderately inhibited the efflux. A one-step spontaneous mutant had...
Fajardo, Daniel A., Cheung, Joyce, Ito, Chikako, Sugawara, Etsuko, Nikaido, Hiroshi, Misra, Rajeev
A novel porin, OmpG, is produced in response to a chromosomal mutation termed cog-192. Molecular characterization of cog-192 revealed that it is a large chromosomal deletion extending from the 3′...
Nikaido, Hiroshi, Basina, Marina, Nguyen, Vy, Rosenberg, Emiko Y.
We found that the previously reported SS-B drug-supersusceptible mutant of Salmonella typhimurium (S. Sukupolvi, M. Vaara, I. M. Helander, P. Viljanen, and P. H. Mäkelä, J. Bacteriol....
Export of autotransported proteins proceeds through an oligomeric ring shaped byC-terminal domains
Veiga, Esteban, Sugawara, Etsuko, Nikaido, Hiroshi, De Lorenzo, Víctor, Fernández, Luis Angel
An investigation was made into the oligomerization, the ability to form pores and the secretion-related properties of the 45 kDa C-terminal domain of the IgA protease (C-IgAP) from Neisseria...
Baranova, Natalya, Nikaido, Hiroshi
Screening of random fragments of Escherichia coli genomic DNA for their ability to increase the novobiocin resistance of a hypersusceptible ΔacrAB mutant resulted in the isolation of a plasmid...
Elkins, Christopher A., Nikaido, Hiroshi
AcrAB-TolC is a constitutively expressed, tripartite efflux transporter complex that functions as the primary resistance mechanism to lipophilic drugs, dyes, detergents, and bile acids in Escherichia...
Li, Xian-Zhi, Poole, Keith, Nikaido, Hiroshi
Of the six putative small multidrug resistance (SMR) family proteins of Pseudomonas aeruginosa, a protein encoded by the PA4990 gene (emrEPae) shows the highest identity to the well-characterized...
Singh, Shiva P., Williams, Yvonne U., Miller, Stephanie, Nikaido, Hiroshi
We examined the way the major outer membrane protein OmpA of Salmonella enterica serovar Typhimurium is recognized by the mouse immune system, by raising a panel of 12 monoclonal antibodies (MAbs)...
Mutants of Escherichia coli That Are Resistant to Certain Beta-Lactam Compounds Lack the ompF Porin
Harder, Karen J., Nikaido, Hiroshi, Matsuhashi, Michio
Carbenicillin-resistant mutants of Escherichia coli K-12 and B/r were found to produce greatly diminished levels of the porin coded by the ompF gene. Physiological and ecological implications of...
Elkins, Christopher A., Nikaido, Hiroshi
AcrAB-TolC is the major, constitutively expressed efflux protein complex that provides resistance to a variety of antimicrobial agents in Escherichia coli. Previous studies showed that AcrA, a...
SEMIROUGH STRAINS OF SALMONELLA
Naide, Yorio, Nikaido, Hiroshi, Mäkelä, P. Helena, Wilkinson, R. G., Stocker, B. A. D.
Luckey, Mary, Nikaido, Hiroshi
Mutant and wild-type LamB proteins (phage λ receptor proteins) were purified by affinity chromatography with immobilized maltose-binding protein, and their transport functions were tested in...
Biosynthesis of T1 Antigen in Salmonella: Origin of d-Galactofuranose and d-Ribofuranose Residues
Sarvas, Matti, Nikaido, Hiroshi
The “T1 side chain” portion of cell wall lipopolysaccharide from T1 strains of Salmonella contains d-galactofuranose and d-ribofuranose residues. Isotope labeling studies, using intact cells of...
Biosynthesis of T1 Antigen in Salmonella: Biosynthesis in a Cell-Free System
Nikaido, Hiroshi, Sarvas, Matti
A particulate fraction from a T1 form of Salmonella typhimurium incorporated radioactivity from uridine diphosphate (UDP)-14C-glucose into products associated with the particulate enzyme. A major...
Kita, Hiroshi, Nikaido, Hiroshi
A major portion of cell wall lipopolysaccharide from group A, group B, or group D1Salmonella corresponds to a linear polysaccharide chain, which consists of α-d-galactosyl-(1 → 2)-α-d-mannosyl-(1...
Ames, Giovanna Ferro-Luzzi, Spudich, Elena Negri, Nikaido, Hiroshi
The protein composition of the outer membrane of Salmonella typhimurium has been analyzed by electrophoresis on slabs of sodium dodecyl sulfate-acrylamide gel. This powerful technique allows very...
Dartigalongue, Claire, Nikaido, Hiroshi, Raina, Satish
Disulfide bond formation in Escherichia coli is a catalyzed reaction accomplished by DsbA. We found that null mutations in a new porin gene, ompL, allowed a total bypass of the DsbA requirement for...
Molecular Basis of Bacterial Outer Membrane Permeability Revisited
Gram-negative bacteria characteristically are surrounded by an additional membrane layer, the outer membrane. Although outer membrane components often play important roles in the interaction of...
Yuasa, Ryosuke, Levinthal, Mark, Nikaido, Hiroshi
A mutant of Salmonella typhimurium LT2 was found to be unable to convert cytidine diphospho-4-keto-6-deoxy-d-glucose into cytidine diphosphoabequose. The mutation maps in the rfb gene cluster, which...
Genetic Determination of Enzymes Synthesizing O-Specific Sugars of Salmonella Lipopolysaccharides
Nikaido, Hiroshi, Nikaido, Kishiko, Mäkelä, P. Helena
Nikaido, Hiroshi (Massachusetts General Hospital, Boston, Mass.), Kishiko Nikaido, and P. Helena Mäkelä. Genetic determination of enzymes synthesizing O-specific sugars of Salmonella...
Efflux Pump-Mediated Intrinsic Drug Resistance in Mycobacterium smegmatis
Li, Xian-Zhi, Zhang, Li, Nikaido, Hiroshi
The Mycobacterium smegmatis genome contains many genes encoding putative drug efflux pumps. Yet with the exception of lfrA, it is not clear whether these genes contribute to the intrinsic drug...
Dartigalongue, Claire, Nikaido, Hiroshi, Raina, Satish
Correction to: The EMBO Journal (2000) 19, 5980–5988. doi: 10.1093/emboj/19.22.5980
Aires, Julio Ramos, Nikaido, Hiroshi
To understand better the mechanisms of resistance-nodulation-division (RND)-type multidrug efflux pumps, we examined the Escherichia coli AcrD pump, whose typical substrates, aminoglycosides, are not...
Yu, Edward W., Aires, Julio R., McDermott, Gerry, Nikaido, Hiroshi
The Escherichia coli AcrB multidrug efflux pump is a membrane protein that recognizes many structurally dissimilar toxic compounds. We previously reported the X-ray structures of four AcrB-ligand...
Bypassing the periplasm: Reconstitution of the AcrAB multidrug efflux pump of Escherichia coli
Zgurskaya, Helen I., Nikaido, Hiroshi
AcrAB is a constitutively expressed, major multidrug efflux system of Escherichia coli. We have purified the cytoplasmic membrane component, AcrB, to near homogeneity, and reconstituted the protein...
Mycolic acids are a major constituent of the mycobacterial cell wall, and they form an effective permeability barrier to protect mycobacteria from antimicrobial agents. Although the chemical...
Aires, Julio Ramos, Köhler, Thilo, Nikaido, Hiroshi, Plésiat, Patrick
A mutant, named 11B, hypersusceptible to aminoglycosides, tetracycline, and erythromycin was isolated after Tn501 insertion mutagenesis of Pseudomonas aeruginosa PAO1. Cloning and sequencing...
Mazzariol, Annarita, Cornaglia, Giuseppe, Nikaido, Hiroshi
The roles of the AmpC chromosomal β-lactamase and the AcrAB efflux system in levels of intrinsic resistance and susceptibility of Escherichia coli to β-lactams were studied with a set of isogenic...
Mazzariol, Annarita, Tokue, Yutaka, Kanegawa, Tiffany M., Cornaglia, Giuseppe, Nikaido, Hiroshi
Immunoblotting with antibody against AcrA, an obligatory component of the AcrAB multidrug efflux system, showed that this protein was overexpressed by ≥170% in 9 of 10 clinical isolates of...
Raimondi, Alessandro, Sisto, Francesca, Nikaido, Hiroshi
Starting from a clinical isolate of Serratia marcescens that produced a chromosomally encoded AmpC β-lactamase inducibly, we isolated by stepwise selection two laboratory mutants that showed high...
Miyamae, Shin, Ueda, Ohmi, Yoshimura, Fuminobu, Hwang, Jaiweon, Tanaka, Yoshinobu, Nikaido, Hiroshi
We cloned a gene, bexA, that codes for a multidrug efflux transporter from the chromosomal DNA of Bacteroides thetaiotaomicron ATCC 29741 by using an Escherichia coli ΔacrAB ΔacrEF mutant as a...
Zhang, Ying, Scorpio, Angelo, Nikaido, Hiroshi, Sun, Zhonghe
Pyrazinamide (PZA) is an important antituberculosis drug. Unlike most antibacterial agents, PZA, despite its remarkable in vivo activity, has no activity against Mycobacterium tuberculosis in vitro...
AcrD of Escherichia coli Is an Aminoglycoside Efflux Pump
Rosenberg, Emiko Y., Ma, Dzwokai, Nikaido, Hiroshi
AcrD, a transporter belonging to the resistance-nodulation-division family, was shown to participate in the efflux of aminoglycosides. Deletion of the acrD gene decreased the MICs of amikacin,...
Zgurskaya, Helen I., Nikaido, Hiroshi
In Escherichia coli, the intrinsic levels of resistance to multiple antimicrobial agents are produced through expression of the three-component multidrug efflux system AcrAB-TolC. AcrB is a...
Active Efflux of Norfloxacin by Bacteroides fragilis
Miyamae, Shin, Nikaido, Hiroshi, Tanaka, Yoshinobu, Yoshimura, Fuminobu
Norfloxacin was actively pumped out by Bacteroides fragilis, which is intrinsically resistant to most fluoroquinolones. Reserpine moderately inhibited the efflux. A one-step spontaneous mutant had...
Fajardo, Daniel A., Cheung, Joyce, Ito, Chikako, Sugawara, Etsuko, Nikaido, Hiroshi, Misra, Rajeev
A novel porin, OmpG, is produced in response to a chromosomal mutation termed cog-192. Molecular characterization of cog-192 revealed that it is a large chromosomal deletion extending from the 3′...
Nikaido, Hiroshi, Basina, Marina, Nguyen, Vy, Rosenberg, Emiko Y.
We found that the previously reported SS-B drug-supersusceptible mutant of Salmonella typhimurium (S. Sukupolvi, M. Vaara, I. M. Helander, P. Viljanen, and P. H. Mäkelä, J. Bacteriol....
Export of autotransported proteins proceeds through an oligomeric ring shaped byC-terminal domains
Veiga, Esteban, Sugawara, Etsuko, Nikaido, Hiroshi, De Lorenzo, Víctor, Fernández, Luis Angel
An investigation was made into the oligomerization, the ability to form pores and the secretion-related properties of the 45 kDa C-terminal domain of the IgA protease (C-IgAP) from Neisseria...
Baranova, Natalya, Nikaido, Hiroshi
Screening of random fragments of Escherichia coli genomic DNA for their ability to increase the novobiocin resistance of a hypersusceptible ΔacrAB mutant resulted in the isolation of a plasmid...
Elkins, Christopher A., Nikaido, Hiroshi
AcrAB-TolC is a constitutively expressed, tripartite efflux transporter complex that functions as the primary resistance mechanism to lipophilic drugs, dyes, detergents, and bile acids in Escherichia...
Li, Xian-Zhi, Poole, Keith, Nikaido, Hiroshi
Of the six putative small multidrug resistance (SMR) family proteins of Pseudomonas aeruginosa, a protein encoded by the PA4990 gene (emrEPae) shows the highest identity to the well-characterized...
Singh, Shiva P., Williams, Yvonne U., Miller, Stephanie, Nikaido, Hiroshi
We examined the way the major outer membrane protein OmpA of Salmonella enterica serovar Typhimurium is recognized by the mouse immune system, by raising a panel of 12 monoclonal antibodies (MAbs)...
Mutants of Escherichia coli That Are Resistant to Certain Beta-Lactam Compounds Lack the ompF Porin
Harder, Karen J., Nikaido, Hiroshi, Matsuhashi, Michio
Carbenicillin-resistant mutants of Escherichia coli K-12 and B/r were found to produce greatly diminished levels of the porin coded by the ompF gene. Physiological and ecological implications of...
Elkins, Christopher A., Nikaido, Hiroshi
AcrAB-TolC is the major, constitutively expressed efflux protein complex that provides resistance to a variety of antimicrobial agents in Escherichia coli. Previous studies showed that AcrA, a...
SEMIROUGH STRAINS OF SALMONELLA
Naide, Yorio, Nikaido, Hiroshi, Mäkelä, P. Helena, Wilkinson, R. G., Stocker, B. A. D.
Luckey, Mary, Nikaido, Hiroshi
Mutant and wild-type LamB proteins (phage λ receptor proteins) were purified by affinity chromatography with immobilized maltose-binding protein, and their transport functions were tested in...
Biosynthesis of T1 Antigen in Salmonella: Origin of d-Galactofuranose and d-Ribofuranose Residues
Sarvas, Matti, Nikaido, Hiroshi
The “T1 side chain” portion of cell wall lipopolysaccharide from T1 strains of Salmonella contains d-galactofuranose and d-ribofuranose residues. Isotope labeling studies, using intact cells of...
Biosynthesis of T1 Antigen in Salmonella: Biosynthesis in a Cell-Free System
Nikaido, Hiroshi, Sarvas, Matti
A particulate fraction from a T1 form of Salmonella typhimurium incorporated radioactivity from uridine diphosphate (UDP)-14C-glucose into products associated with the particulate enzyme. A major...
Kita, Hiroshi, Nikaido, Hiroshi
A major portion of cell wall lipopolysaccharide from group A, group B, or group D1Salmonella corresponds to a linear polysaccharide chain, which consists of α-d-galactosyl-(1 → 2)-α-d-mannosyl-(1...
Ames, Giovanna Ferro-Luzzi, Spudich, Elena Negri, Nikaido, Hiroshi
The protein composition of the outer membrane of Salmonella typhimurium has been analyzed by electrophoresis on slabs of sodium dodecyl sulfate-acrylamide gel. This powerful technique allows very...
Dartigalongue, Claire, Nikaido, Hiroshi, Raina, Satish
Disulfide bond formation in Escherichia coli is a catalyzed reaction accomplished by DsbA. We found that null mutations in a new porin gene, ompL, allowed a total bypass of the DsbA requirement for...
Molecular Basis of Bacterial Outer Membrane Permeability Revisited
Gram-negative bacteria characteristically are surrounded by an additional membrane layer, the outer membrane. Although outer membrane components often play important roles in the interaction of...
Yuasa, Ryosuke, Levinthal, Mark, Nikaido, Hiroshi
A mutant of Salmonella typhimurium LT2 was found to be unable to convert cytidine diphospho-4-keto-6-deoxy-d-glucose into cytidine diphosphoabequose. The mutation maps in the rfb gene cluster, which...
Genetic Determination of Enzymes Synthesizing O-Specific Sugars of Salmonella Lipopolysaccharides
Nikaido, Hiroshi, Nikaido, Kishiko, Mäkelä, P. Helena
Nikaido, Hiroshi (Massachusetts General Hospital, Boston, Mass.), Kishiko Nikaido, and P. Helena Mäkelä. Genetic determination of enzymes synthesizing O-specific sugars of Salmonella...
Efflux Pump-Mediated Intrinsic Drug Resistance in Mycobacterium smegmatis
Li, Xian-Zhi, Zhang, Li, Nikaido, Hiroshi
The Mycobacterium smegmatis genome contains many genes encoding putative drug efflux pumps. Yet with the exception of lfrA, it is not clear whether these genes contribute to the intrinsic drug...
Dartigalongue, Claire, Nikaido, Hiroshi, Raina, Satish
Correction to: The EMBO Journal (2000) 19, 5980–5988. doi: 10.1093/emboj/19.22.5980
Aires, Julio Ramos, Nikaido, Hiroshi
To understand better the mechanisms of resistance-nodulation-division (RND)-type multidrug efflux pumps, we examined the Escherichia coli AcrD pump, whose typical substrates, aminoglycosides, are not...
Yu, Edward W., Aires, Julio R., McDermott, Gerry, Nikaido, Hiroshi
The Escherichia coli AcrB multidrug efflux pump is a membrane protein that recognizes many structurally dissimilar toxic compounds. We previously reported the X-ray structures of four AcrB-ligand...
Takatsuka, Yumiko, Nikaido, Hiroshi
Escherichia coli AcrB is a multidrug efflux transporter that recognizes multiple toxic chemicals and expels them from cells. It is a proton antiporter belonging to the resistance-nodulation-division...
Conformation of the AcrB Multidrug Efflux Pump in Mutants of the Putative Proton Relay Pathway
Su, Chih-Chia, Li, Ming, Gu, Ruoyu, Takatsuka, Yumiko, McDermott, Gerry, Nikaido, Hiroshi, ...
We previously reported the X-ray structures of wild-type Escherichia coli AcrB, a proton motive force-dependent multidrug efflux pump, and its N109A mutant. These structures presumably reflect the...
Structural and Functional Analyses of the Major Outer Membrane Protein of Chlamydia trachomatis▿
Sun, Guifeng, Pal, Sukumar, Sarcon, Annahita K., Kim, Soyoun, Sugawara, Etsuko, Nikaido, Hiroshi, ...
Chlamydia trachomatis is a major pathogen throughout the world, and preventive measures have focused on the production of a vaccine using the major outer membrane protein (MOMP). Here, in elementary...
Murata, Takeshi, Tseng, Will, Guina, Tina, Miller, Samuel I., Nikaido, Hiroshi
The PhoPQ two-component system of Salmonella enterica serovar Typhimurium produces a remodeling of the lipid A domain of the lipopolysaccharide, including the PagP-catalyzed addition of palmitoyl...
Takatsuka, Yumiko, Nikaido, Hiroshi
Escherichia coli AcrB is a multidrug efflux transporter that recognizes multiple toxic chemicals having diverse structures. Recent crystallographic studies of the asymmetric trimer of AcrB suggest...
Takatsuka, Yumiko, Nikaido, Hiroshi
Escherichia coli AcrB is a proton motive force-dependent multidrug efflux transporter that recognizes multiple toxic chemicals having diverse structures. Recent crystallographic studies of the...
Kinetic behavior of the major multidrug efflux pump AcrB of Escherichia coli
Nagano, Keiji, Nikaido, Hiroshi
Multidrug efflux transporters, especially those that belong to the resistance-nodulation-division (RND) family, often show very broad substrate specificity and play a major role both in the intrinsic...