Hiroshi Nojima

Focused Microarray Analysis of Peripheral Mononuclear Blood Cells from Churg-Strauss Syndrome Patients (2008)

Tougan, Takahiro, Onda, Hiroaki, Okuzaki, Daisuke, Kobayashi, Shigeto, Hashimoto, Hiroshi, Nojima, Hiroshi

DNA diagnostics are useful but are hampered by difficult ethical issues. Moreover, it cannot provide enough information on the environmental factors that are important for pathogenesis of certain...

Expression Profiling of PBMC-based Diagnostic Gene Markers Isolated from Vasculitis Patients (2008)

Kobayashi, Shigeto, Ito, Akihiko, Okuzaki, Daisuke, Onda, Hiroaki, Yabuta, Norikazu, Nagamori, Ippei, ...

Vasculitis (angiitis) is a systemic autoimmune disease that often causes fatal symptoms. We aimed to isolate cDNA markers that would be useful for diagnosing not only vasculitis but also other...

Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification (2008)

Tougan, Takahiro, Okuzaki, Daisuke, Nojima, Hiroshi

Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is...

Meiosis specific coiled-coil proteins in Shizosaccharomyces pombe (2007)

Ohtaka, Ayami, Saito, Takamune T, Okuzaki, Daisuke, Nojima, Hiroshi

Abstract Many meiosis-specific proteins in Schizosaccharomyces pombe contain coiled-coil motifs which play essential roles for meiotic progression. For example, the coiled-coil motifs present in...

Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest (2006)

Yabuta, Norikazu, Onda, Hiroaki, Watanabe, Masafumi, Yoshioka, Naohisa, Nagamori, Ippei, Funatsu, Tomoyuki, ...

We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found...

Isolation and Expression Profiling of Genes Upregulated in Bone Marrow-Derived Mononuclear Cells of Rheumatoid Arthritis Patients (2006)

Nakamura, Nobuo, Shimaoka, Yasunori, Tougan, Takahiro, Onda, Hiroaki, Okuzaki, Daisuke, Zhao, Hanjun, ...

We have comprehensively identified the genes whose expressions are augmented in bone marrow-derived mononuclear cells (BMMC) from patients with Rheumatoid Arthritis (RA) as compared with BMMCs from...

Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest (2006)

Yabuta, Norikazu, Onda, Hiroaki, Watanabe, Masafumi, Yoshioka, Naohisa, Nagamori, Ippei, Funatsu, Tomoyuki, ...

We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found...

Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest (2006)

Yabuta, Norikazu, Onda, Hiroaki, Watanabe, Masafumi, Yoshioka, Naohisa, Nagamori, Ippei, Funatsu, Tomoyuki, ...

We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found...

Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest (2006)

Yabuta, Norikazu, Onda, Hiroaki, Watanabe, Masafumi, Yoshioka, Naohisa, Nagamori, Ippei, Funatsu, Tomoyuki, ...

We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found...

Isolation and Expression Profiling of Genes Upregulated in the Peripheral Blood Cells of Systemic Lupus Erythematosus Patients (2006)

Ishii, Taeko, Onda, Hiroaki, Tanigawa, Akie, Ohshima, Shiro, Fujiwara, Hiroshi, Mima, Toru, ...

We have identified the genes whose expressions are augmented in the blood cells of the patients with systemic lupus erythematosus (SLE) using the ‘stepwise subtraction’ technique along with...

A positive feedback loop between the p53 and Lats2 tumor suppressors prevents tetraploidization (2006)

Aylon, Yael, Michael, Dan, Shmueli, Ayelet, Yabuta, Norikazu, Nojima, Hiroshi, Oren, Moshe

Damage to the mitotic spindle and centrosome dysfunction can lead to cancer. To prevent this, cells trigger a succession of checkpoint responses, where an initial mitotic delay is followed by...

Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest (2006)

Yabuta, Norikazu, Onda, Hiroaki, Watanabe, Masafumi, Yoshioka, Naohisa, Nagamori, Ippei, Funatsu, Tomoyuki, ...

We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found...

Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest (2006)

Yabuta, Norikazu, Onda, Hiroaki, Watanabe, Masafumi, Yoshioka, Naohisa, Nagamori, Ippei, Funatsu, Tomoyuki, ...

We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found...

Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest (2006)

Yabuta, Norikazu, Onda, Hiroaki, Watanabe, Masafumi, Yoshioka, Naohisa, Nagamori, Ippei, Funatsu, Tomoyuki, ...

We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found...

A derivative of oleamide potently inhibits the spontaneous metastasis of mouse melanoma BL6 cells (2004)

Ito, Akihiko, Morita, Nobuyoshi, Miura, Daisaku, Koma, Yu-ichiro, Kataoka, Tatsuki R., Yamasaki, Hiroshi, ...

We reported previously that the abnormally augmented expression of connexin 26 (Cx26) is responsible for the enhanced spontaneous metastasis of mouse BL6 melanoma cells, and that the exogenous...

A derivative of oleamide potently inhibits the spontaneous metastasis of mouse melanoma BL6 cells (2004)

Ito, Akihiko, Morita, Nobuyoshi, Miura, Daisaku, Koma, Yu-ichiro, Kataoka, Tatsuki R., Yamasaki, Hiroshi, ...

We previously reported that the abnormally augmented expression of connexin 26 (Cx26) is responsible for the enhanced spontaneous metastasis of mouse BL6 melanoma cells, and that the exogenous...

Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination (2004)

Saito, Takamune T., Tougan, Takahiro, Kasama, Takashi, Okuzaki, Daisuke, Nojima, Hiroshi

We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein...

Abundant Poly(A)-Bearing RNAs That Lack Open Reading Frames in Schizosaccharomyces pombe (2002)

Watanabe, Takanori, Miyashita, Kazuyuki, Saito, Takamune T., Nabeshima, Kentaro, Nojima, Hiroshi

We report here that 6.9% (68/987) of randomly selected cDNA clones from an S. pombe cDNA library lack apparently long open reading frames which we denote prl. One of them, prl1, was examined further...

Comprehensive isolation of meiosis-specific genes identifies novel proteins and unusual non-coding transcripts in Schizosaccharomyces pombe (2001)

Watanabe, Takanori, Miyashita, Kazuyuki, Saito, Takamune T., Yoneki, Takahiro, Kakihara, Yoshito, Nabeshima, Kentaro, ...

In order to isolate meiosis-specific genes in Schizosaccharomyces pombe, we have constructed a subtracted cDNA library enriched in clones whose expression is enhanced during meiosis induced by...

Dmc1 of Schizosaccharomyces pombe plays a role in meiotic recombination (2000)

Fukushima, Kentaro, Tanaka, Yoshimi, Nabeshima, Kentaro, Yoneki, Takahiro, Tougan, Takahiro, Tanaka, Seiji, ...

We report here a Schizosaccharomyces pombe gene (dmc1+) that resembles budding yeast DMC1 in the region immediately upstream of the rad24+ gene. We showed by northern and Southern blot...

Identification of Open Reading Frames in Schizosaccharomyces pombe cDNAs (1997)

Yoshioka, Sachiyo, Kato, Kikuya, Nakai, Kenta, Okayama, Hiroto, Nojima, Hiroshi

A total of 214 non-overlapping cDNA clones from Schizosaccharomyces pombe were selected and completely sequenced. The clones not previously reported were divided into the following three groups: 1)...

SCR: novel human suppressors of cdc2/cdc13 mutants of Schizosaccharomyces pombe harbour motifs for RNA binding proteins (1994)

Kanaoka, Yoshihide, Nojima, Hiroshi

By phenotypic complementation of the cdc2 and the cdc13 mutants of the fission yeast Schizosaccharomyces pombe, we have cloned two novel multicopy suppressors from a cDNA library of the human...

Hac1: A novel yeast bZIP protein binding to the CRE motif is a multicopy suppressor for cdcW mutant of Schizosaccharomyces pombe (1994)

Nojima, Hiroshi, Leem, Sun-Hee, Araki, Hiroyuki, Sakai, Akira, Nakashima, Naomi, Kanaoka, Yoshihide, ...

We cloned by phenotypic complementation a novel Saccharomyces cerevlslae's multicopy suppressor of the Schizosaccharomyces pombe cddO-129 mutant which we call HAC1, an acronym of 'homologous to...

A simple treatment of DNA in a ligation mixture prior to electroporation improves transformation frequency (1993)

Kobori, Masato, Nojima, Hiroshi

Molecular cloning and sequence analysis of human Na, K-ATPase {beta}-subunit (1986)

Kawakami, Kiyoshi, Nojima, Hiroshi, Ohta, Toshiko, Nagano, Kei

We have isolated a cDNA clone for the β-subunit of HeLa cell Na, K–ATPase, containing a 2208-base- pair cDNA insert covering the whole coding region of the β-subunit. Nucleotide sequence analysis...

Primary Structure of the {alpha}-Subunit of Human Na, K-ATPase Deduced from cDNA Sequence (1986)

KAWAKAMI, Kiyoshi, OHTA, Toshiko, NOJIMA, Hiroshi, NAGANO, Kei

Clones carrying cDNA sequences for the α-subunit of the Na, K-ATPase from HeLa cells have been isolated. Nucleotide sequence analysis of the cloned cDNA has revealed the primary structure of this...

Close Structural Resemblance among Small Nuclear RNAs (1983)

NOJIMA, Hiroshi

The published primary and secondary structures of small nuclear RNAs (snRNAs) (1–9) were reexamined and a new common model for their secondary structures was proposed. What is surprising is the...

Purification and Properties of Phosphoglycerate Kinase from Thermus thermophilus Strain HB8 (1979)

NOJIMA, Hiroshi, OSHIMA, Tairo, NODA, Haruhiko

A glycolytic enzyme, phosphoglycerate kinase [EC 2.7.2.3], was purified from cells of an extreme thermophile, Thermus thermophilus strain HB8. The enzyme was resistant to heat, and no loss of...

Replication Factor C3 of Schizosaccharomyces pombe, a Small Subunit of Replication Factor C Complex, Plays a Role in Both Replication and Damage Checkpoints

Shimada, Midori, Okuzaki, Daisuke, Tanaka, Seiji, Tougan, Takahiro, Tamai, Katsuyuki K., Shimoda, Chikashi, ...

We report here the isolation and functional analysis of the rfc3+ gene of Schizosaccharomyces pombe, which encodes the third subunit of replication factor C (RFC3). Because the rfc3+ gene was...

Comprehensive isolation of meiosis-specific genes identifies novel proteins and unusual non-coding transcripts in Schizosaccharomyces pombe

Watanabe, Takanori, Miyashita, Kazuyuki, Saito, Takamune T., Yoneki, Takahiro, Kakihara, Yoshito, Nabeshima, Kentaro, ...

In order to isolate meiosis-specific genes in Schizosaccharomyces pombe, we have constructed a subtracted cDNA library enriched in clones whose expression is enhanced during meiosis induced by...

Dmc1 of Schizosaccharomyces pombe plays a role in meiotic recombination

Fukushima, Kentaro, Tanaka, Yoshimi, Nabeshima, Kentaro, Yoneki, Takahiro, Tougan, Takahiro, Tanaka, Seiji, ...

We report here a Schizosaccharomyces pombe gene (dmc1+) that resembles budding yeast DMC1 in the region immediately upstream of the rad24+ gene. We showed by northern and Southern blot analysis that...

Isolation of Transformation Suppressor Genes by cDNA Subtraction: Lumican Suppresses Transformation Induced by v-src and v-K-ras

Yoshioka, Naohisa, Inoue, Hirokazu, Nakanishi, Kazuyoshi, Oka, Kiyomasa, Yutsudo, Masuo, Yamashita, Atsuko, ...

We have reported that suppressive factors for transformation by viral oncogenes are expressed in primary rat embryo fibroblasts (REFs). To identify such transformation suppressor genes, we prepared a...

A novel meiosis-specific protein of fission yeast, Meu13p, promotes homologous pairing independently of homologous recombination

Nabeshima, Kentaro, Kakihara, Yoshito, Hiraoka, Yasushi, Nojima, Hiroshi

Meiotic homologous pairing is crucial to proper homologous recombination, which secures subsequent reductional chromosome segregation. We have identified a novel meiosis-specific protein of fission...

The meiotic recombination checkpoint is regulated by checkpoint rad+ genes in fission yeast

Shimada, Midori, Nabeshima, Kentaro, Tougan, Takahiro, Nojima, Hiroshi

During the course of meiotic prophase, intrinsic double-strand breaks (DSBs) must be repaired before the cell can engage in meiotic nuclear division. Here we investigate the mechanism that controls...

A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation

Ito, Akihiko, Kataoka, Tatsuki R., Watanabe, Masafumi, Nishiyama, Kazutaka, Mazaki, Yuichi, Sabe, Hisataka, ...

Both F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. Retrotransposon insertion was found...

A role for heterologous gap junctions between melanoma and endothelial cells in metastasis

Ito, Akihiko, Katoh, Fumitaka, Kataoka, Tatsuki R., Okada, Morihito, Tsubota, Noriaki, Asada, Hideo, ...

F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. We found that connexin (Cx) 26 is...

Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination

Saito, Takamune T., Tougan, Takahiro, Kasama, Takashi, Okuzaki, Daisuke, Nojima, Hiroshi

We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein...

Use of stepwise subtraction to comprehensively isolate mouse genes whose transcription is up-regulated during spermiogenesis

Fujii, Takayuki, Tamura, Kazutoshi, Masai, Kumiko, Tanaka, Hiromitsu, Nishimune, Yoshitake, Nojima, Hiroshi

We report the isolation of 153 mouse genes whose expression is dramatically up-regulated during spermiogenesis. We used a novel variation of the subtractive hybridization technique called stepwise...

Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest

Yabuta, Norikazu, Onda, Hiroaki, Watanabe, Masafumi, Yoshioka, Naohisa, Nagamori, Ippei, Funatsu, Tomoyuki, ...

We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found...

Spo5/Mug12, a Putative Meiosis-Specific RNA-Binding Protein, Is Essential for Meiotic Progression and Forms Mei2 Dot-Like Nuclear Foci†

Kasama, Takashi, Shigehisa, Akira, Hirata, Aiko, Saito, Takamune T., Tougan, Takahiro, Okuzaki, Daisuke, ...

We report here a functional analysis of spo5+(mug12+) of Schizosaccharomyces pombe, which encodes a putative RNA-binding protein. The disruption of spo5+ caused abnormal sporulation, generating...

オーチャードグラスの貯蔵炭水化物の差が刈取後の窒素吸収に及ぼす影響

大泉, 久一, オオイズミ, ヒサカズ, Ōizumi, Hisakazu, OIZUMI, Hisakazu, 池和田, 寿, イケワダ, ヒサシ, ...

オーチャードグラスの品種フロードの1クローンを水耕法により栽培し, 刈取前5日間の遮光処理と刈取高さの差により,...

Replication Factor C3 of Schizosaccharomyces pombe, a Small Subunit of Replication Factor C Complex, Plays a Role in Both Replication and Damage Checkpoints

Shimada, Midori, Okuzaki, Daisuke, Tanaka, Seiji, Tougan, Takahiro, Tamai, Katsuyuki K., Shimoda, Chikashi, ...

We report here the isolation and functional analysis of the rfc3+ gene of Schizosaccharomyces pombe, which encodes the third subunit of replication factor C (RFC3). Because the rfc3+ gene was...

Comprehensive isolation of meiosis-specific genes identifies novel proteins and unusual non-coding transcripts in Schizosaccharomyces pombe

Watanabe, Takanori, Miyashita, Kazuyuki, Saito, Takamune T., Yoneki, Takahiro, Kakihara, Yoshito, Nabeshima, Kentaro, ...

In order to isolate meiosis-specific genes in Schizosaccharomyces pombe, we have constructed a subtracted cDNA library enriched in clones whose expression is enhanced during meiosis induced by...

Dmc1 of Schizosaccharomyces pombe plays a role in meiotic recombination

Fukushima, Kentaro, Tanaka, Yoshimi, Nabeshima, Kentaro, Yoneki, Takahiro, Tougan, Takahiro, Tanaka, Seiji, ...

We report here a Schizosaccharomyces pombe gene (dmc1+) that resembles budding yeast DMC1 in the region immediately upstream of the rad24+ gene. We showed by northern and Southern blot analysis that...

Isolation of Transformation Suppressor Genes by cDNA Subtraction: Lumican Suppresses Transformation Induced by v-src and v-K-ras

Yoshioka, Naohisa, Inoue, Hirokazu, Nakanishi, Kazuyoshi, Oka, Kiyomasa, Yutsudo, Masuo, Yamashita, Atsuko, ...

We have reported that suppressive factors for transformation by viral oncogenes are expressed in primary rat embryo fibroblasts (REFs). To identify such transformation suppressor genes, we prepared a...

A novel meiosis-specific protein of fission yeast, Meu13p, promotes homologous pairing independently of homologous recombination

Nabeshima, Kentaro, Kakihara, Yoshito, Hiraoka, Yasushi, Nojima, Hiroshi

Meiotic homologous pairing is crucial to proper homologous recombination, which secures subsequent reductional chromosome segregation. We have identified a novel meiosis-specific protein of fission...

The meiotic recombination checkpoint is regulated by checkpoint rad+ genes in fission yeast

Shimada, Midori, Nabeshima, Kentaro, Tougan, Takahiro, Nojima, Hiroshi

During the course of meiotic prophase, intrinsic double-strand breaks (DSBs) must be repaired before the cell can engage in meiotic nuclear division. Here we investigate the mechanism that controls...

A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation

Ito, Akihiko, Kataoka, Tatsuki R., Watanabe, Masafumi, Nishiyama, Kazutaka, Mazaki, Yuichi, Sabe, Hisataka, ...

Both F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. Retrotransposon insertion was found...

A role for heterologous gap junctions between melanoma and endothelial cells in metastasis

Ito, Akihiko, Katoh, Fumitaka, Kataoka, Tatsuki R., Okada, Morihito, Tsubota, Noriaki, Asada, Hideo, ...

F10 and BL6 sublines of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells are metastatic after subcutaneous injection. We found that connexin (Cx) 26 is...

Mcp7, a meiosis-specific coiled-coil protein of fission yeast, associates with Meu13 and is required for meiotic recombination

Saito, Takamune T., Tougan, Takahiro, Kasama, Takashi, Okuzaki, Daisuke, Nojima, Hiroshi

We previously showed that Meu13 of Schizosaccharomyces pombe functions in homologous pairing and recombination at meiosis I. Here we show that a meiosis-specific gene encodes a coiled-coil protein...

Use of stepwise subtraction to comprehensively isolate mouse genes whose transcription is up-regulated during spermiogenesis

Fujii, Takayuki, Tamura, Kazutoshi, Masai, Kumiko, Tanaka, Hiromitsu, Nishimune, Yoshitake, Nojima, Hiroshi

We report the isolation of 153 mouse genes whose expression is dramatically up-regulated during spermiogenesis. We used a novel variation of the subtractive hybridization technique called stepwise...

Spo5/Mug12, a Putative Meiosis-Specific RNA-Binding Protein, Is Essential for Meiotic Progression and Forms Mei2 Dot-Like Nuclear Foci†

Kasama, Takashi, Shigehisa, Akira, Hirata, Aiko, Saito, Takamune T., Tougan, Takahiro, Okuzaki, Daisuke, ...

We report here a functional analysis of spo5+(mug12+) of Schizosaccharomyces pombe, which encodes a putative RNA-binding protein. The disruption of spo5+ caused abnormal sporulation, generating...

Isolation and characterization of the TIGA genes, whose transcripts are induced by growth arrest

Yabuta, Norikazu, Onda, Hiroaki, Watanabe, Masafumi, Yoshioka, Naohisa, Nagamori, Ippei, Funatsu, Tomoyuki, ...

We report here the isolation of 44 genes that are upregulated after serum starvation and/or contact inhibition. These genes have been termed TIGA, after Transcript Induced by Growth Arrest. We found...

オーチャードグラスの貯蔵炭水化物の差が刈取後の窒素吸収に及ぼす影響

大泉, 久一, 池和田, 寿, 野島, 博, オオイズミ, ヒサカズ, Ōizumi, Hisakazu, イケワダ, ヒサシ, ...

オーチャードグラスの品種フロードの1クローンを水耕法により栽培し, 刈取前5日間の遮光処理と刈取高さの差により,...

ソルガム茎片の側芽伸長に及ぼす外与サイトカイニンの影響

野島, 博, ノジマ, ヒロシ, Nojima, Hiroshi, NOJIMA, Hiroshi, 大泉, 久一, オオイズミ, ヒサカズ, ...

ソルガムの刈取後の再生における植物ホルモンの影響を明らかにするため本実験を行った.根及び葉鞘を切除した茎基部, 長さ約15cmの茎片で,...

パタロブトラゾール処理がラッカセイの乾物分配および葉群構造に及ぼす影響

礒田, 昭弘, イソダ, アキヒロ, Isoda, Akihiro, 中里, 浩司, ナカザト, ヒロシ, Nakazato, Hiroshi, ...

ラッカセイの茎葉の過繁茂を抑制し, 子実への乾物分配を高める目的で, ジベレリン生合成阻害剤であるパクロブトラゾールを用い,...

ソルガム茎片の側芽伸長に及ぼす外与サイトカイニンの影響

野島, 博, 大泉, 久一, 中澤, まどか, ノジマ, ヒロシ, Nojima, Hiroshi, オオイズミ, ヒサカズ, ...

ソルガムの刈取後の再生における植物ホルモンの影響を明らかにするため本実験を行った.根及び葉鞘を切除した茎基部, 長さ約15cmの茎片で,...

パタロブトラゾール処理がラッカセイの乾物分配および葉群構造に及ぼす影響

礒田, 昭弘, 中里, 浩司, 野島, 博, イソダ, アキヒロ, ナカザト, ヒロシ, ノジマ, ヒロシ, ...

ラッカセイの茎葉の過繁茂を抑制し, 子実への乾物分配を高める目的で, ジベレリン生合成阻害剤であるパクロブトラゾールを用い,...

園芸作物の品質評価と経済的評価に関する研究 : 第1報 メロンの食味評価と化学的・物理的特性の関係

野島, 博, ノジマ, ヒロシ, Nojima, Hiroshi, NOJIMA, Hiroshi, 丸尾, 達, マルオ, トオル, ...

マスクメロンの客観的品質評価として炭水化物(単少糖類), タンパク質量, インピーダンスを測定し,...

刈取後の高温がソルガムの枯死・再生に及ぼす影響

高崎, 康夫, タカサキ,ヤスオ, Takasaki,Yasuo, TAKASAKI, Yasuo, 御手洗, 昌寛, ミタライ, マサヒロ, ...

1.刈取後の高温がソルガムの枯死・再生に及ぼす影響を知ろうとしてこの実験を行った.雪印ハイブリッド・ソルゴーをポット栽培し,...

園芸作物の品質評価と経済的評価に関する研究 : 第1報 メロンの食味評価と化学的・物理的特性の関係

野島, 博, ノジマ, ヒロシ, Nojima, Hiroshi, NOJIMA, Hiroshi, 丸尾, 達, マルオ, トオル, ...

マスクメロンの客観的品質評価として炭水化物(単少糖類), タンパク質量, インピーダンスを測定し,...

刈取後の高温がソルガムの枯死・再生に及ぼす影響

高崎, 康夫, タカサキ,ヤスオ, Takasaki,Yasuo, TAKASAKI, Yasuo, 御手洗, 昌寛, ミタライ, マサヒロ, ...

1.刈取後の高温がソルガムの枯死・再生に及ぼす影響を知ろうとしてこの実験を行った.雪印ハイブリッド・ソルゴーをポット栽培し,...

オーチャードグラスの貯蔵炭水化物の差が刈取後の窒素吸収に及ぼす影響

大泉, 久一, オオイズミ, ヒサカズ, Ōizumi, Hisakazu, OIZUMI, Hisakazu, 池和田, 寿, イケワダ, ヒサシ, ...

オーチャードグラスの品種フロードの1クローンを水耕法により栽培し, 刈取前5日間の遮光処理と刈取高さの差により,...

ソルガム茎片の側芽伸長に及ぼす外与サイトカイニンの影響

野島, 博, ノジマ, ヒロシ, Nojima, Hiroshi, NOJIMA, Hiroshi, 大泉, 久一, オオイズミ, ヒサカズ, ...

ソルガムの刈取後の再生における植物ホルモンの影響を明らかにするため本実験を行った.根及び葉鞘を切除した茎基部, 長さ約15cmの茎片で,...

パタロブトラゾール処理がラッカセイの乾物分配および葉群構造に及ぼす影響

礒田, 昭弘, イソダ, アキヒロ, Isoda, Akihiro, 中里, 浩司, ナカザト, ヒロシ, Nakazato, Hiroshi, ...

ラッカセイの茎葉の過繁茂を抑制し, 子実への乾物分配を高める目的で, ジベレリン生合成阻害剤であるパクロブトラゾールを用い,...

大豆の生育初期における根粒除去処理が窒素固定能に及ぼす影響

大泉, 久一, オオイズミ, ヒサカズ, Ōizumi, Hisakazu, OIZUMI, Hisakazu, 岩井, 一弥, イワイ, カズヤ, ...

大豆(品種ホウギョク)を供試し, 生育初期に根粒を除去した区と, 除去しない対照区を設け, 根粒数, 根粒生体重, 葉面積, 地上部乾物重,...

反覆刈取処理がオーチャードグラスの再生に及ぼす影響

野島, 博, ノジマ, ヒロシ, Nojima, Hiroshi, NOJIMA, Hiroshi, 大泉, 久一, オイズミ, ヒサカズ, ...

オーチャードグラスの再生に及ぼす刈株中の貯蔵炭水化物(TNC)の影響をみるため, 反覆刈取処理を行い, 刈株中のTNC量に差をつけ,...

施設栽培土壤の塩類集積対策としてのイネ科飼料作物の利用 : 第1報 塩類濃度を異にする土壤における夏期短期栽培イネ科飼料作物の生育

大泉, 久一, オオイズミ, ヒサカズ, Ōizumi, Hisakazu, OIZUMI, Hisakazu, 高崎, 康夫, タカサキ,ヤスオ, ...

1.夏期の2ヶ月間休耕されるハウス内土壤の除塩を目的としたイネ科飼料作物の短期栽培について検討した.2.トウモロコシ, ソルゴー, シコクビエ,...

施設栽培土壤の塩類集積対策としてのイネ科飼料作物の利用 : 第2報 イネ科飼料作物による塩類の吸収及び収穫物の土壤中での分解

嶋田, 典司, シマダ, ノリツグ, Shimada, Noritsugu, SHIMADA, Noritsugu, 佐藤, 幸夫, サトウ, ユキオ, ...

施設栽培でしばしば問題となる土壤の塩類集積を防止する対策の一つとして,...

飼料作物の発芽・初期生育に及ぼす発芽中の水分ストレスの影響 : 第2報 マメ科草種について

大泉, 久一, オオイズミ, ヒサカズ, Ōizumi, Hisakazu, OIZUMI, Hisakazu, 三平, 東作, ミヒラ, トウサク, ...

牧草類の播種直後の早ばつの後影響を知るため, マメ科草種, ダイズ, ベッチ, レンゲ, アルファルファ, アカクローバ, アルサイククローバ,...

大豆の生育初期における根粒除去処理が窒素固定能に及ぼす影響

大泉, 久一, オオイズミ, ヒサカズ, Ōizumi, Hisakazu, OIZUMI, Hisakazu, 岩井, 一弥, イワイ, カズヤ, ...

大豆(品種ホウギョク)を供試し, 生育初期に根粒を除去した区と, 除去しない対照区を設け, 根粒数, 根粒生体重, 葉面積, 地上部乾物重,...

反覆刈取処理がオーチャードグラスの再生に及ぼす影響

野島, 博, ノジマ, ヒロシ, Nojima, Hiroshi, NOJIMA, Hiroshi, 大泉, 久一, オイズミ, ヒサカズ, ...

オーチャードグラスの再生に及ぼす刈株中の貯蔵炭水化物(TNC)の影響をみるため, 反覆刈取処理を行い, 刈株中のTNC量に差をつけ,...

生育にともなうソルガムの側芽伸長能力の変化

高崎, 康夫, タカサキ,ヤスオ, Takasaki,Yasuo, TAKASAKI, Yasuo, 大泉, 久一, オオイズミ, ヒサカズ, ...

1.飼料用ソルガムの側芽伸長能力の生育時期による違いを,...

簡易積算日射計フィルムによるイネ群落の受光態勢の解析

礒田, 昭弘, イソダ, アキヒロ, Isoda, Akihiro, ISODA, Akihiro, 吉村, 登雄, ヨシムラ, タカオ, ...

色素の退色率から日射量を測定する簡易積算日射計フィルムを用い,...

施設栽培土壤の塩類集積対策としてのイネ科飼料作物の利用 : 第1報 塩類濃度を異にする土壤における夏期短期栽培イネ科飼料作物の生育

大泉, 久一, オオイズミ, ヒサカズ, Ōizumi, Hisakazu, OIZUMI, Hisakazu, 高崎, 康夫, タカサキ,ヤスオ, ...

1.夏期の2ヶ月間休耕されるハウス内土壤の除塩を目的としたイネ科飼料作物の短期栽培について検討した.2.トウモロコシ, ソルゴー, シコクビエ,...

施設栽培土壤の塩類集積対策としてのイネ科飼料作物の利用 : 第2報 イネ科飼料作物による塩類の吸収及び収穫物の土壤中での分解

嶋田, 典司, シマダ, ノリツグ, Shimada, Noritsugu, SHIMADA, Noritsugu, 佐藤, 幸夫, サトウ, ユキオ, ...

施設栽培でしばしば問題となる土壤の塩類集積を防止する対策の一つとして,...

飼料作物の発芽・初期生育に及ぼす発芽中の水分ストレスの影響 : 第2報 マメ科草種について

大泉, 久一, オオイズミ, ヒサカズ, Ōizumi, Hisakazu, OIZUMI, Hisakazu, 三平, 東作, ミヒラ, トウサク, ...

牧草類の播種直後の早ばつの後影響を知るため, マメ科草種, ダイズ, ベッチ, レンゲ, アルファルファ, アカクローバ, アルサイククローバ,...

生育にともなうソルガムの側芽伸長能力の変化

高崎, 康夫, タカサキ,ヤスオ, Takasaki,Yasuo, TAKASAKI, Yasuo, 大泉, 久一, オオイズミ, ヒサカズ, ...

1.飼料用ソルガムの側芽伸長能力の生育時期による違いを,...

簡易積算日射計フィルムによるイネ群落の受光態勢の解析

礒田, 昭弘, イソダ, アキヒロ, Isoda, Akihiro, ISODA, Akihiro, 吉村, 登雄, ヨシムラ, タカオ, ...

色素の退色率から日射量を測定する簡易積算日射計フィルムを用い,...

ラッカセイの葉群構造と着莢位置

礒田, 昭弘, イソダ, アキヒロ, Isoda, Akihiro, ISODA, Akihiro, 野島, 博, ノジマ, ヒロシ, ...

ラッカセイの草型の異なる7品種(千葉43号, 千葉半立, ナカテユタカ, タチマサリ, 334A, 白油7-3, バレンシア)を用い,...

ラッカセイの葉群構造と着莢位置

礒田, 昭弘, イソダ, アキヒロ, Isoda, Akihiro, ISODA, Akihiro, 野島, 博, ノジマ, ヒロシ, ...

ラッカセイの草型の異なる7品種(千葉43号, 千葉半立, ナカテユタカ, タチマサリ, 334A, 白油7-3, バレンシア)を用い,...

A positive feedback loop between the p53 and Lats2 tumor suppressors prevents tetraploidization

Aylon, Yael, Michael, Dan, Shmueli, Ayelet, Yabuta, Norikazu, Nojima, Hiroshi, Oren, Moshe

Damage to the mitotic spindle and centrosome dysfunction can lead to cancer. To prevent this, cells trigger a succession of checkpoint responses, where an initial mitotic delay is followed by...

Meiosis specific coiled-coil proteins in Shizosaccharomyces pombe

Ohtaka, Ayami, Saito, Takamune T, Okuzaki, Daisuke, Nojima, Hiroshi

Many meiosis-specific proteins in Schizosaccharomyces pombe contain coiled-coil motifs which play essential roles for meiotic progression. For example, the coiled-coil motifs present in Meu13 and...

Increased Expression of a Nucleolar Nop5/Sik Family Member in Metastatic Melanoma Cells : Evidence for Its Role in Nucleolar Sizing and Function

Nakamoto, Ken’i, Ito, Akihiko, Watabe, Kenji, Koma, Yu-ichiro, Asada, Hideo, Yoshikawa, Kunihiko, ...

F10 and BL6 cells of B16 mouse melanoma cells are metastatic after intravenous injection, but only BL6 cells can metastasize to lungs after subcutaneous injection. Differences in gene expression...

A Truncated Isoform of the Protein Phosphatase 2A B56γ Regulatory Subunit May Promote Genetic Instability and Cause Tumor Progression

Ito, Akihiko, Koma, Yu-ichiro, Watabe, Kenji, Nagano, Teruaki, Endo, Yuichi, Nojima, Hiroshi, ...

F10, a subline of the B16 mouse melanoma cell line, is itself the parent of the more metastatic BL6 line. BL6 cells differ from F10 cells by an alteration of the gene encoding the B56γ regulatory...

Localization of the PP2A B56γ Regulatory Subunit at the Golgi Complex : Possible Role in Vesicle Transport and Migration

Ito, Akihiko, Koma, Yu-ichiro, Sohda, Miwa, Watabe, Kenji, Nagano, Teruaki, Misumi, Yoshio, ...

The BL6 subline was derived from the F10 line, which was derived from the B16 mouse melanoma cell line. BL6 cells are more invasive than F10 cells and differ genetically from F10 cells by an...

Mcp4, a Meiotic Coiled-Coil Protein, Plays a Role in F-Actin Positioning during Schizosaccharomyces pombe Meiosis▿

Ohtaka, Ayami, Okuzaki, Daisuke, Saito, Takamune T., Nojima, Hiroshi

Some meiosis-specific proteins of Schizosaccharomyces pombe harbor coiled-coil motifs and play essential roles in meiotic progression. Here we describe Mcp4, a novel meiosis-specific protein whose...

Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast

Saito, Takamune T., Okuzaki, Daisuke, Nojima, Hiroshi

During meiotic prophase I of the fission yeast Schizosaccharomyces pombe, oscillatory nuclear movement occurs. This promotes homologous chromosome pairing and recombination and involves cortical...

Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification

Tougan, Takahiro, Okuzaki, Daisuke, Nojima, Hiroshi

Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is...

Expression Profiling of PBMC-based Diagnostic Gene Markers Isolated from Vasculitis Patients

Kobayashi, Shigeto, Ito, Akihiko, Okuzaki, Daisuke, Onda, Hiroaki, Yabuta, Norikazu, Nagamori, Ippei, ...

Vasculitis (angiitis) is a systemic autoimmune disease that often causes fatal symptoms. We aimed to isolate cDNA markers that would be useful for diagnosing not only vasculitis but also other...

Focused Microarray Analysis of Peripheral Mononuclear Blood Cells from Churg–Strauss Syndrome Patients

Tougan, Takahiro, Onda, Hiroaki, Okuzaki, Daisuke, Kobayashi, Shigeto, Hashimoto, Hiroshi, Nojima, Hiroshi

DNA diagnostics are useful but are hampered by difficult ethical issues. Moreover, it cannot provide enough information on the environmental factors that are important for pathogenesis of certain...