Hiroyuki Nakai

Large-scale molecular characterization of adeno-associated virus vector integration in mouse liver (2008)

Hiroyuki Nakai, Xiaolin Wu, Sally Fuess, Theresa A. Storm, David Munroe, Eugenio Montini, ...

Recombinant adeno-associated virus (rAAV) vector holds promise for gene therapy. Despite a low frequency of chromosomal integration of vector genomes, recent studies have raised concerns about the...

Function-unknown Glycoside Hydrolase Family 31 Proteins, mRNAs of which were Expressed in Rice Ripening and Germinating Stages, are {alpha}-Glucosidase and {alpha}-Xylosidase (2007)

Nakai, Hiroyuki, Tanizawa, Shigeki, Ito, Tatsuya, Kamiya, Koutarou, Kim, Young-Min, Yamamoto, Takeshi, ...

In rice (Oryza sativa L., var Nipponbare) seeds, there were three mRNAs encoding for function-unknown hydrolase family 31 homologous proteins (ONGX-H1, ONGX-H3 and ONGX-H4): ONGX-H1 mRNA was...

Multiple forms of α-glucosidase in rice seeds (Oryza sativa L., var Nipponbare) (2007)

Nakai, Hiroyuki, Ito, Tatsuya, Hayashi, Masatoshi, Kamiya, Koutarou, Yamamoto, Takeshi, Matsubara, Kazuki, ...

Two isoforms of α-glucosidases (ONG2-I and ONG2-II) were purified from dry rice seeds (Oryza sativa L., var Nipponbare). Both ONG2-I and ONG2-II were the gene products of ONG2 mRNA expressed in...

Multiple forms of α-glucosidase in rice seeds (Oryza sativa L., var Nipponbare) (2007)

Nakai, Hiroyuki, Ito, Tatsuya, Hayashi, Masatoshi, Kamiya, Koutarou, Yamamoto, Takeshi, Matsubara, Kazuki, ...

Two isoforms of α-glucosidases (ONG2-I and ONG2-II) were purified from dry rice seeds (Oryza sativa L., var Nipponbare). Both ONG2-I and ONG2-II were the gene products of ONG2 mRNA expressed in...

Purification and Characterization of α-Glucosidase I from Japanese Honeybee (Apis cerana japonica) and Molecular Cloning of Its cDNA (2006)

Wongchawalit, Jintanart, Yamamoto, Takeshi, Nakai, Hiroyuki, Kim, Young-Min, Sato, Natsuko, Nishimoto, Mamoru, ...

α-Glucosidase (JHGase I) was purified from a Japanese subspecies of eastern honeybee (Apis cerana japonica) as an electrophoretically homogeneous protein. Enzyme activity of the crude extract was...

Purification and Characterization of α-Glucosidase I from Japanese Honeybee (Apis cerana japonica) and Molecular Cloning of Its cDNA (2006)

Wongchawalit, Jintanart, Yamamoto, Takeshi, Nakai, Hiroyuki, Kim, Young-Min, Sato, Natsuko, Nishimoto, Mamoru, ...

α-Glucosidase (JHGase I) was purified from a Japanese subspecies of eastern honeybee (Apis cerana japonica) as an electrophoretically homogeneous protein. Enzyme activity of the crude extract was...

Environment manipulation planner for humanoid robots using task graph that generates action sequence (2004)

Kei Okada, Atsushi Haneda, Hiroyuki Nakai, Masayuki Inaba, Hirochika Inoue

Abstract — In this paper, we describe a planner for a humanoid robot that is capable of finding a path in an environment with movable objects, whereas previous motion planner only deal with an...

Coexpression of factor VIII heavy and light chain adeno-associated viral vectors produces biologically active protein

Burton, Melissa, Nakai, Hiroyuki, Colosi, Peter, Cunningham, Janet, Mitchell, Rachel, Couto, Linda

We are interested in using recombinant adeno-associated viral vectors in the treatment of hemophilia A. Because of the size constraints of recombinant adeno-associated viral vectors, we delivered the...

Nonrandom Transduction of Recombinant Adeno-Associated Virus Vectors in Mouse Hepatocytes In Vivo: Cell Cycling Does Not Influence Hepatocyte Transduction

Miao, Carol H., Nakai, Hiroyuki, Thompson, Arthur R., Storm, Theresa A., Chiu, Winnie, Snyder, Richard O., ...

Recombinant adeno-associated virus vectors (rAAV) show promise in preclinical trials for the treatment of genetic diseases including hemophilia. Liver-directed gene transfer results in a slow rise in...

Recruitment of Single-Stranded Recombinant Adeno-Associated Virus Vector Genomes and Intermolecular Recombination Are Responsible for Stable Transduction of Liver In Vivo

Nakai, Hiroyuki, Storm, Theresa A., Kay, Mark A.

Recombinant adeno-associated virus (rAAV) vectors stably transduce hepatocytes in experimental animals. Following portal-vein administration of rAAV vectors in vivo, single-stranded (ss) rAAV genomes...

Isolation of Recombinant Adeno-Associated Virus Vector-Cellular DNA Junctions from Mouse Liver

Nakai, Hiroyuki, Iwaki, Yuichi, Kay, Mark A., Couto, Linda B.

Recombinant adeno-associated virus (rAAV) vectors allow for sustained expression of transgene products from mouse liver following a single portal vein administration. Here a rAAV vector expressing...

Extrachromosomal Recombinant Adeno-Associated Virus Vector Genomes Are Primarily Responsible for Stable Liver Transduction In Vivo

Nakai, Hiroyuki, Yant, Stephen R., Storm, Theresa A., Fuess, Sally, Meuse, Leonard, Kay, Mark A.

Recombinant adeno-associated virus (rAAV) vectors stably transduce hepatocytes in experimental animals. Although the vector genomes are found both as extrachromosomes and as chromosomally integrated...

A Limited Number of Transducible Hepatocytes Restricts a Wide-Range Linear Vector Dose Response in Recombinant Adeno-Associated Virus-Mediated Liver Transduction

Nakai, Hiroyuki, Thomas, Clare E., Storm, Theresa A., Fuess, Sally, Powell, Sharon, Wright, J. Fraser, ...

Recombinant adeno-associated virus (rAAV) vectors are promising vehicles for achieving stable liver transduction in vivo. However, the mechanisms of liver transduction are not fully understood, and...

Unrestricted Hepatocyte Transduction with Adeno-Associated Virus Serotype 8 Vectors in Mice

Nakai, Hiroyuki, Fuess, Sally, Storm, Theresa A., Muramatsu, Shin-ichi, Nara, Yuko, Kay, Mark A.

Recombinant adeno-associated virus (rAAV) vectors can mediate long-term stable transduction in various target tissues. However, with rAAV serotype 2 (rAAV2) vectors, liver transduction is confined to...

Large-Scale Molecular Characterization of Adeno-Associated Virus Vector Integration in Mouse Liver

Nakai, Hiroyuki, Wu, Xiaolin, Fuess, Sally, Storm, Theresa A., Munroe, David, Montini, Eugenio, ...

Recombinant adeno-associated virus (rAAV) vector holds promise for gene therapy. Despite a low frequency of chromosomal integration of vector genomes, recent studies have raised concerns about the...

Liver Transduction with Recombinant Adeno-Associated Virus Is Primarily Restricted by Capsid Serotype Not Vector Genotype

Grimm, Dirk, Pandey, Kusum, Nakai, Hiroyuki, Storm, Theresa A., Kay, Mark A.

We and others have recently reported highly efficient liver gene transfer with adeno-associated virus 8 (AAV-8) pseudotypes, i.e., AAV-2 genomes packaged into AAV-8 capsids. Here we studied whether...

Coexpression of factor VIII heavy and light chain adeno-associated viral vectors produces biologically active protein

Burton, Melissa, Nakai, Hiroyuki, Colosi, Peter, Cunningham, Janet, Mitchell, Rachel, Couto, Linda

We are interested in using recombinant adeno-associated viral vectors in the treatment of hemophilia A. Because of the size constraints of recombinant adeno-associated viral vectors, we delivered the...

Nonrandom Transduction of Recombinant Adeno-Associated Virus Vectors in Mouse Hepatocytes In Vivo: Cell Cycling Does Not Influence Hepatocyte Transduction

Miao, Carol H., Nakai, Hiroyuki, Thompson, Arthur R., Storm, Theresa A., Chiu, Winnie, Snyder, Richard O., ...

Recombinant adeno-associated virus vectors (rAAV) show promise in preclinical trials for the treatment of genetic diseases including hemophilia. Liver-directed gene transfer results in a slow rise in...

Recruitment of Single-Stranded Recombinant Adeno-Associated Virus Vector Genomes and Intermolecular Recombination Are Responsible for Stable Transduction of Liver In Vivo

Nakai, Hiroyuki, Storm, Theresa A., Kay, Mark A.

Recombinant adeno-associated virus (rAAV) vectors stably transduce hepatocytes in experimental animals. Following portal-vein administration of rAAV vectors in vivo, single-stranded (ss) rAAV genomes...

Isolation of Recombinant Adeno-Associated Virus Vector-Cellular DNA Junctions from Mouse Liver

Nakai, Hiroyuki, Iwaki, Yuichi, Kay, Mark A., Couto, Linda B.

Recombinant adeno-associated virus (rAAV) vectors allow for sustained expression of transgene products from mouse liver following a single portal vein administration. Here a rAAV vector expressing...

Extrachromosomal Recombinant Adeno-Associated Virus Vector Genomes Are Primarily Responsible for Stable Liver Transduction In Vivo

Nakai, Hiroyuki, Yant, Stephen R., Storm, Theresa A., Fuess, Sally, Meuse, Leonard, Kay, Mark A.

Recombinant adeno-associated virus (rAAV) vectors stably transduce hepatocytes in experimental animals. Although the vector genomes are found both as extrachromosomes and as chromosomally integrated...

A Limited Number of Transducible Hepatocytes Restricts a Wide-Range Linear Vector Dose Response in Recombinant Adeno-Associated Virus-Mediated Liver Transduction

Nakai, Hiroyuki, Thomas, Clare E., Storm, Theresa A., Fuess, Sally, Powell, Sharon, Wright, J. Fraser, ...

Recombinant adeno-associated virus (rAAV) vectors are promising vehicles for achieving stable liver transduction in vivo. However, the mechanisms of liver transduction are not fully understood, and...

Unrestricted Hepatocyte Transduction with Adeno-Associated Virus Serotype 8 Vectors in Mice

Nakai, Hiroyuki, Fuess, Sally, Storm, Theresa A., Muramatsu, Shin-ichi, Nara, Yuko, Kay, Mark A.

Recombinant adeno-associated virus (rAAV) vectors can mediate long-term stable transduction in various target tissues. However, with rAAV serotype 2 (rAAV2) vectors, liver transduction is confined to...

Large-Scale Molecular Characterization of Adeno-Associated Virus Vector Integration in Mouse Liver

Nakai, Hiroyuki, Wu, Xiaolin, Fuess, Sally, Storm, Theresa A., Munroe, David, Montini, Eugenio, ...

Recombinant adeno-associated virus (rAAV) vector holds promise for gene therapy. Despite a low frequency of chromosomal integration of vector genomes, recent studies have raised concerns about the...

Liver Transduction with Recombinant Adeno-Associated Virus Is Primarily Restricted by Capsid Serotype Not Vector Genotype

Grimm, Dirk, Pandey, Kusum, Nakai, Hiroyuki, Storm, Theresa A., Kay, Mark A.

We and others have recently reported highly efficient liver gene transfer with adeno-associated virus 8 (AAV-8) pseudotypes, i.e., AAV-2 genomes packaged into AAV-8 capsids. Here we studied whether...

DNA Palindromes with a Modest Arm Length of ≳20 Base Pairs Are a Significant Target for Recombinant Adeno-Associated Virus Vector Integration in the Liver, Muscles, and Heart in Mice▿

Inagaki, Katsuya, Lewis, Susanna M., Wu, Xiaolin, Ma, Congrong, Munroe, David J., Fuess, Sally, ...

Our previous study has shown that recombinant adeno-associated virus (rAAV) vector integrates preferentially in genes, near transcription start sites and CpG islands in mouse liver (H. Nakai, X. Wu,...

The Role of DNA-PKcs and Artemis in Opening Viral DNA Hairpin Termini in Various Tissues in Mice▿

Inagaki, Katsuya, Ma, Congrong, Storm, Theresa A., Kay, Mark A., Nakai, Hiroyuki

A subset of cellular DNA hairpins at double-strand breaks is processed by DNA-dependent protein kinase catalytic subunit (DNA-PKcs)- and Artemis-associated endonuclease. DNA hairpin termini of...

Frequency and Spectrum of Genomic Integration of Recombinant Adeno-Associated Virus Serotype 8 Vector in Neonatal Mouse Liver▿

Inagaki, Katsuya, Piao, Chuncheng, Kotchey, Nicole M., Wu, Xiaolin, Nakai, Hiroyuki

Neonatal injection of recombinant adeno-associated virus serotype 8 (rAAV8) vectors results in widespread transduction in multiple organs and therefore holds promise in neonatal gene therapy. On the...