To study autoantibodies against liver cell surface membrane clinically, anti-LP-1 and anti-Tamm-Horsfall glycoprotein (THGP) were determined in the sera of patients with various liver diseases. They...
Hasui, Toshimi, Shimomura, Hiroyuki, Tsuji, Hideyuki, Wato, Masaki, Tsuji, Takao
Recently, factors predicting the response to interferon (IFN) therapy against hepatitis C virus (HCV) have received much attention. To evaluate the usefulness of the quantitation of intrahepatic HCV...
Tsuji, Hideyuki, Shimomura, Hiroyuki, Wato, Masaki, Kondo, Junichi, Tsuji, Takao
To study the virological and serological characteristics of asymptomatic hepatitis C virus (HCV) carriers, 165 blood donors positive for antibody against HCV proteins by the second generation assay,...
Wato, Masaki, Shimomura, Hiroyuki, Fujio, Kozo, Tsuji, Hideyuki, Kondo, Junichi, Fujioka, Shin-ichi, ...
We purified an apurinic/apyrimidinic (AP) endonuclease from mouse ascites sarcoma (SR-C3H/He) cells. The enzyme showed nicking activity on acid-depurinated DNA but not on untreated, intact DNA. It...
Detection of negative strand RNA of hepatitis C virus in infected liver and serum. (2007)
Nakagawa, Hiroshi, Shimomura, Hiroyuki, Hasui, Toshimi, Tsuji, Hideyuki, Tsuji, Takao
The negative strand RNA of hepatitis C virus, supposed to be a replicative intermediate of the virus appears to indicate viral replication. In this study, we detected the negative strand RNA by using...
Takahashi, Michiko, Yamada, Gotaro, Miyamoto, Rieko, Doi, Toshihiko, Endo, Hisashi, Nishimoto, Hiroshi, ...
We measured hepatitis C virus antibody titers in 13 patients with chronic hepatitis C to determine whether titration of hepatitis C virus antibody was useful or not, to predict and evaluate the...
Morii, Kazuhiko, Shimomura, Hiroyuki, Nakagawa, Hiroshi, Hasui, Toshimi, Tsuji, Takao
Since detection of hepatitis C virus RNA by the polymerase chain reaction (PCR) showed that there existed anti-C100-3 (anti-HCV) antibody negative patients infected with HCV, we attempted to find out...
Genetic variation of putative core gene in hepatitis C virus. (2007)
Fujio, Kozo, Shimomura, Hiroyuki, Tsuji, Takao
Genetic variation of hepatitis C virus was assessed. We prepared RNA fractions from 21 patients' sera which were positive for hepatitis C virus RNA, synthesized their cDNAs, and amplified fragments,...
Relationship of serum markers of hepatitis B and C virus replication in coinfected patients. (2007)
Tsuji, Hideyuki, Shimomura, Hiroyuki, Fujio, Kozo, Wato, Masaki, Kondo, Junichi, Hasui, Toshimi, ...
To evaluate viral interference between hepatitis B and C, we studied coinfected patients serologically and molecular biologically. Twenty-seven patients positive for hepatitis B surface antigen...
Nakajima, Hirofumi, Shimomura, Hiroyuki, Iwasaki, Yoshiaki, Ikeda, Fusao, Umeoka, Fumi, Chengyu, Piao, ...
To improve the efficacy of interferon (IFN) treatment for chronic hepatitis C, we have proposed the twice-daily administration of IFN-beta as a promising induction therapy. In this study, we...
Kondo, Junichi, Shimomura, Hiroyuki, Fujioka, Shin-ichi, Iwasaki, Yoshiaki, Takagi, Shinjiro, Ohnishi, Yasuhiro, ...
The preS2 region of the hepatitis B virus (HBV) has been reported to have human polymerized albumin receptor (PAR) activity, which correlates with viral replication. Here, we studied the genomic...
Ariyoshi, Masanori, MIzuno, Motowo, Morisue, Yoshiko, Shimada, Morizou, Fujita, Shirou, Nasu, Junichirou, ...
We developed a monoclonal antibody (MoAb) (clone 5E8) against an antigen on the bile canalicular membrane of rat hepatocyte. By immunoblotting, MoAb 5E8 detected a band of 110 kD. In this study, we...
Ohya, Shogen, Mizuno, Motowo, Kawada, Mikihiro, Nasu, Junichirou, Okada, Hiroyuki, Shimomura, Hiroyuki, ...
We have previously developed an enzyme-linked immunosorbent assay (ELISA) to measure stool decay-accelerating factor (DAF) and found that stool DAF concentrations were significantly elevated in...
Alam, Shahjalal S., Nakamura, Takashi, Naganuma, Atsushi, Nozaki, Akito, Nouso, Kazuhiro, Shimomura, Hiroyuki, ...
We have shown that highly proofreading DNA polymerase is required for the polymerase chain reaction in the genetic analysis of hepatitis C virus (HCV). To clarify the status of HCV quasispecies in...
Hirai, Michio, Mizuno, Motowo, Morisue, Yoshiko, Yoshioka, Masao, Shimada, Morizou, Nasu, Junichirou, ...
Anti-idiotype antibodies (Ab2) play an important role in the homeostasis of immune responses and are related to the development and the disease activity of certain autoimmune diseases. The...
Ishii, Yasushi, Shimomura, Hiroyuki, Ito, Mamoru, Miyake, Masanobu, Ikeda, Fusao, Miyake, Jiro, ...
It has been documented that the serum complement activities measured by hemolytic assay (CH50) are decreased after storage of sera at a low temperature in some patients with chronic hepatitis C....