Hisashi Matsushima

Regulation of Chloroplast Gene Expression is Affected in ali, a Novel Tobacco Albino Mutant (2001)

Bae, Chang-Hyu, Abe, Tomoko, Matsuyama, Tomoki, Fukunishi, Nobuhisa, Nagata, Noriko, Nakano, Takeshi, ...

An albino mutant designated ali was isolated from an M2population derived from a tobacco (Nicotiana tabacum L. ‘BY-4’) plant which had been irradiated with a14N-ion beam at an early...

Development of {beta}-1,3-glucanase activity in germinated tomato seeds (2000)

Morohashi, Yukio, Matsushima, Hisashi

Laminarin‐hydrolysing activity developed in the endosperm of tomato (Lycopersicon esculentum) seeds following germination. The enzyme was basic (pI>10) and the apparent...

Water stress enhances {beta}-amylase activity in cucumber cotyledons (2000)

Todaka, Daisuke, Matsushima, Hisashi, Morohashi, Yukio

Cotyledons detached from 4‐d‐old cucumber (Cucumis sativus L.) seedlings were subjected to water stress (air‐drying or PEG‐treatment) to examine the...

Colour scanning electron microscopy of peltate glandular trichomes of fresh developing hops (Humulus lupulus L.) (1998)

Yamada, Mitsuhiko, Suzuki, Takeo, Oho, Eisaku, Matsushima, Hisashi

Morphological and colour changes of fresh developing hop female cones were examined using a colour scanning electron microscopy technique. A highlight component of a video microscope image was...

SEM Observation and HPLC Analysis of the Accumulation of Alpha- and Beta-acids in the Fresh Developing Hop (Humulus lupulus L.) Peltate Glandular Trichomes (1995)

Hirosawa, Takayasu, Saito, Tomoko, Tanaka, Tetsuo, Matsushima, Hisashi

Immediately after fresh hop developing peltate glandular trichomes were examined and photographed in a few minutes with an SEM equipped with a rapid pre-evacuating system, hop bitter resins (alpha-...

Preparation of Plant Protoplasts for SEM Observation by t-Butanol Freeze-Drying Method (1990)

Kaneko, Yasuko, Matsushima, Hisashi, Sekine, Michiyo, Matsumoto, Kenji

Plant protoplasts made from leaf parenchyma cells were prepared for SEM observation using a t-butanol freeze-drying method. The smooth surface of the plasma membrane was very well preserved when the...

A Method for the Rapid Growth in Culture of Gametophytes of Equisetum arvense with Antheridia (1989)

Kuriyama, Akira, Hojoh, Toshiyuki, Sugawara, Yasutake, Matsushima, Hisashi, Takeuchi, Masayuki

Rapid growth in culture of Equisetum arvense gametophytes was obtained using Murashige-Skoog's medium plus 3% (w/v) sucrose and continuous illumination. In darkness, growth was reduced and...

Assimilation of carbon dioxide in mesophyll chloroplasts and bundle sheath strands mechanically isolated from corn leaves (1974)

Usuda, Hideaki, Matsushima, Hisashi, Miyachi, Shigetoh

Mesophyll chloroplasts capable of assimilating 1.2 μmoles CO2 per milligram chlorophyll per hour were isolated from 7-day-old corn (Zea mays, Nagano No. 1) leaves. Addition of phosphoenolpyruvate...

The Microbody with a Crystalloid Core in Tobacco Cultured Cell Clone XD-6S: III. Developmental Studies on the Microbody (1972)

MATSUSHIMA, Hisashi

XD–6S cells cultured in Murashige and Skoog’s medium containing kinetin and indole–3–acetic acid (IAA) were harvested at daily intervals and fixed in 5% gtutaialdehyde. Post–fixation was...

The Microbody with a Crystalloid Core in Tobacco Cultured Cel Clone XD-6S: II. The Fine Structure of the Crystalloid (1971)

MATSUSHIMA, Hisashi

Tobacco cultured cell clone XD-6S in medium containing kinetin and indole-3-acetic acid were fixed in 5% glutaraldehyde and post-fixed in 1%osmium tetroxide. Examinations of serial...

The Microbody with a Crystalloid Core in Tobacco Cultured Cell Clone XD-6S I. Cytochemical Studies on the Microbody (1971)

MATSUSHIMA, Hisashi

Tobacco cultured cells of XD-6S were fixed in 5% glutaraldehyde, and then incubated either in medium containing trypsin, RNAse or DNAse, or in medium containing 3,3'-diaminobenzidine (DAB) and...

Control of α-Amylase Development in Cotyledons during and following Germination of Mung Bean Seeds 1

Morohashi, Yukio, Katoh, Hisashi, Kaneko, Yasuko, Matsushima, Hisashi

Developmental patterns of α-amylase in Vigna radiata cotyledons during and following germination were quite different depending on the differences in the treatments of cotyledons during the...

Appearance and Disappearance of Cyanide-Resistant Respiration in Vigna mungo Cotyledons during and following Germination of the Axis

Morohashi, Yukio, Matsushima, Hisashi

Mitochondrial preparations isolated from black gram (Vigna mungo L.) cotyledons exhibited cyanide-resistant respiration which was of mitochondrial origin. The appearance and the disappearance of this...

Control of α-Amylase Development in Cotyledons during and following Germination of Mung Bean Seeds 1

Morohashi, Yukio, Katoh, Hisashi, Kaneko, Yasuko, Matsushima, Hisashi

Developmental patterns of α-amylase in Vigna radiata cotyledons during and following germination were quite different depending on the differences in the treatments of cotyledons during the...

Appearance and Disappearance of Cyanide-Resistant Respiration in Vigna mungo Cotyledons during and following Germination of the Axis

Morohashi, Yukio, Matsushima, Hisashi

Mitochondrial preparations isolated from black gram (Vigna mungo L.) cotyledons exhibited cyanide-resistant respiration which was of mitochondrial origin. The appearance and the disappearance of this...