Hitoshi Ueda

Midline governs axon pathfinding by coordinating expression of two major guidance systems (2009)

Liu, Qing-Xin, Hiramoto, Masaki, Ueda, Hitoshi, Gojobori, Takashi, Hiromi, Yasushi, Hirose, Susumu

Formation of the neural network requires concerted action of multiple axon guidance systems. How neurons orchestrate expression of multiple guidance genes is poorly understood. Here, we show that...

Drosophila blimp-1 is a transient transcriptional repressor that controls timing of the ecdysone-induced developmental pathway (2008)

Agawa, Yasuo, Sarhan, Moustafa, Kageyama, Yuji, Akagi, Kazutaka, Takai, Masayoshi, Hashiyama, Kazuya, ...

Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-fl is expressed in a temporally regulated manner following pulses of ecdysteroid...

Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor (2003)

Shimojima, Tsukasa, Okada, Masahiro, Nakayama, Takahiro, Ueda, Hitoshi, Okawa, Katsuya, Iwamatsu, Akihiro, ...

Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates...

Direct Expression of a Synthetic Gene in Escherichia coli: Purification and Physicochemical Properties of Human Initiation Factor 4E (1994)

Morino, Shigenobu, Yasui, Masanobu, Doi, Mitsunobu, Inoue, Masatoshi, Ishida, Toshimasa, Ueda, Hitoshi, ...

An artificial synthetic gene coding for human eIF-4E was cloned into an expression vector and direct expression was attempted in Escherichia coli [BL21(DE3) strain] under the control of T7 promoter....

Expression of a Synthetic Gene for Human Cap Binding Protein (Human IF-4E) in herichia coli and Fluorescence Studies on Interaction with mRNA Cap Structure Analogues (1991)

Ueda, Hitoshi, Maruyama, Hitoshi, Doi, Mitsunobu, Inoue, Masatoshi, Ishida, Toshimasa, Morioka, Hiroshi, ...

An artificial gene coding for the human cap binding protein (hCBP: human IF-4E) was chemically synthesized and expressed in Escherichia coli under the control of a trp promoter. The DNA duplex of 662...

Defining the sequence recognized with BmFTZ-F1, a sequence specific DNA binding factor in the silkworm, Bombyx mori, as revealed by direct sequencing of bound oligonucleotides and gel mobility shift competition analysis (1991)

Ueda, Hitoshi, Hirose, Susumu

BmFTZ-F1 is a Bombyx mori homologue of FTZ-F1, a positive regulator of the fushi tarazu gene of Drosophila melanogaster. In order to determine the sequence recognized with this factor, we made three...

Identification and purification of a Bombyx mori homologue of FTZ-F1 (1990)

Ueda, Hitoshi, Hirose, Susumu

Extracts from embryos and from posterior and middle silk glands of the silkworm, Bombyx mori contain a sequence specific DNA binding factor termed BmFTZ-F1. The factor binds to the recognition site...

Human Mammary Cancer Cell Mutants with Altered Hormone Receptor Activity (1986)

UEDA, Hitoshi, HAGINO, Yoshiaki, ONO, Mayumi, KUWANO, Michihiko

We have recently isolated retinoic acid-resistant clones U-2 and U-3 from human breast cancer cell line MCF-7 (Ueda et al. (1985) Cancer Res. 45, 3332–3338). Growth of MCF-7 cells was found to be...

Multiprotein bridging factor 1 (MBF1) is an evolutionarily conserved transcriptional coactivator that connects a regulatory factor and TATA element-binding protein

Takemaru, Ken-ichi, Li, Feng-Qian, Ueda, Hitoshi, Hirose, Susumu

Multiprotein bridging factor 1 (MBF1) is a transcriptional cofactor that bridges between the TATA box-binding protein (TBP) and the Drosophila melanogaster nuclear hormone receptor FTZ-F1 or its...

Segmentation gene product Fushi tarazu is an LXXLL motif-dependent coactivator for orphan receptor FTZ-F1

Suzuki, Taiga, Kawasaki, Haruhisa, Yu, Ruth T., Ueda, Hitoshi, Umesono, Kazuhiko

Orphan receptors for whom cognate ligands have not yet been identified form a large subclass within the nuclear receptor superfamily. To address one aspect of how they might regulate transcription,...

Yeast Coactivator MBF1 Mediates GCN4-Dependent Transcriptional Activation

Takemaru, Ken-ichi, Harashima, Satoshi, Ueda, Hitoshi, Hirose, Susumu

Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1...

Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor

Shimojima, Tsukasa, Okada, Masahiro, Nakayama, Takahiro, Ueda, Hitoshi, Okawa, Katsuya, Iwamatsu, Akihiro, ...

Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates...

Multiprotein bridging factor 1 (MBF1) is an evolutionarily conserved transcriptional coactivator that connects a regulatory factor and TATA element-binding protein

Takemaru, Ken-ichi, Li, Feng-Qian, Ueda, Hitoshi, Hirose, Susumu

Multiprotein bridging factor 1 (MBF1) is a transcriptional cofactor that bridges between the TATA box-binding protein (TBP) and the Drosophila melanogaster nuclear hormone receptor FTZ-F1 or its...

Segmentation gene product Fushi tarazu is an LXXLL motif-dependent coactivator for orphan receptor FTZ-F1

Suzuki, Taiga, Kawasaki, Haruhisa, Yu, Ruth T., Ueda, Hitoshi, Umesono, Kazuhiko

Orphan receptors for whom cognate ligands have not yet been identified form a large subclass within the nuclear receptor superfamily. To address one aspect of how they might regulate transcription,...

Yeast Coactivator MBF1 Mediates GCN4-Dependent Transcriptional Activation

Takemaru, Ken-ichi, Harashima, Satoshi, Ueda, Hitoshi, Hirose, Susumu

Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1...

Drosophila FACT contributes to Hox gene expression through physical and functional interactions with GAGA factor

Shimojima, Tsukasa, Okada, Masahiro, Nakayama, Takahiro, Ueda, Hitoshi, Okawa, Katsuya, Iwamatsu, Akihiro, ...

Chromatin structure plays a critical role in the regulation of transcription. Drosophila GAGA factor directs chromatin remodeling to its binding sites. We show here that Drosophila FACT (facilitates...

Drosophila Blimp-1 Is a Transient Transcriptional Repressor That Controls Timing of the Ecdysone-Induced Developmental Pathway▿

Agawa, Yasuo, Sarhan, Moustafa, Kageyama, Yuji, Akagi, Kazutaka, Takai, Masayoshi, Hashiyama, Kazuya, ...

Regulatory mechanisms controlling the timing of developmental events are crucial for proper development to occur. ftz-f1 is expressed in a temporally regulated manner following pulses of ecdysteroid...

Midline governs axon pathfinding by coordinating expression of two major guidance systems

Liu, Qing-Xin, Hiramoto, Masaki, Ueda, Hitoshi, Gojobori, Takashi, Hiromi, Yasushi, Hirose, Susumu

Formation of the neural network requires concerted action of multiple axon guidance systems. How neurons orchestrate expression of multiple guidance genes is poorly understood. Here, we show that...