Holger Sültmann

Letter Identification and Classification of Differentially Expressed Genes in Renal Cell Carcinoma by Expression Profiling on a Global Human 31,500-Element cDNA Array (2008)

Judith M. Boer, Wolfgang K. Huber, Holger Sültmann, Friederike Wilmer, Anja Von Heydebreck, Stefan Haas, ...

We investigated the changes in gene expression accompanying the development and progression of kidney cancer by use of 31,500-element complementary DNA arrays. We measured expression profiles for...

Analysis (A. Poustka) DKFZ Heidelberg Acknowledgements (2008)

Wolfgang Huber, Anja Von Heydebreck, Günther Sawitzki, Holger Sültmann, Klaus Steiner, Markus Vogt, ...

First analysis steps o quality control and optimization o calibration and error modeling o data transformations

Predicting pathway membership via domain signatures (2008)

Fröhlich, Holger, Fellmann, Mark, Sültmann, Holger, Poustka, Annemarie, Beißbarth, Tim

Motivation: Functional characterization of genes is of great importance for the understanding of complex cellular processes. Valuable information for this purpose can be obtained from pathway...

Estimating large-scale signaling networks through nested effect models with intervention effects from microarray data (2008)

Fröhlich, Holger, Fellmann, Mark, Sültmann, Holger, Poustka, Annemarie, Beissbarth, Tim

Motivation: Targeted interventions using RNA interference in combination with the measurement of secondary effects with DNA microarrays can be used to computationally reverse engineer features of...

Identification of aberrant chromosomal regions from gene expression microarray studies applied to human breast cancer (2007)

Buness, Andreas, Kuner, Ruprecht, Ruschhaupt, Markus, Poustka, Annemarie, Sültmann, Holger, Tresch, Achim

Motivation: In cancer, chromosomal imbalances like amplifications and deletions, or changes in epigenetic mechanisms like DNA methylation influence the transcriptional activity. These alterations are...

arrayMagic: twocolour cDNA microarray quality control and preprocessing (2005)

Andreas Buness, Wolfgang Huber, Klaus Steiner, Holger Sültmann, Annemarie Poustka

Summary: arrayMagic is a software package for quality control and preprocessing of two-colour cDNA microarray data. The automated analysis pipeline comprises data import, normalization, replica...

Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference genes suitable for normalization (2004)

Haller,Florian, Kulle,Bettina, Schwager,Stefanie, Gunawan,Bastian, Von Heydebreck,Anja, Sültmann,Holger, ...

In quantitative reverse transcription–polymerase chain reaction (qRT–PCR), normalization using reference genes is a common useful approach, but the validation of suitable reference genes remains...

Systematic analysis of T7 RNA polymerase based in vitrolinear RNA amplification for use in microarray experiments (2004)

Schneider, Jörg, Buneß, Andreas, Huber, Wolfgang, Volz, Joachim, Kioschis, Petra, Hafner, Mathias, ...

Abstract Background The requirement of a large amount of high-quality RNA is a major limiting factor for microarray experiments using biopsies. An average microarray experiment requires 10–100 μg...

Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference genes suitable for normalization (2004)

Haller, Florian, Kulle, Bettina, Schwager, Stefanie, Gunawan, Bastian, Von Heydebreck, Anja, Sültmann, Holger, ...

In quantitative reverse transcription–polymerase chain reaction (qRT–PCR), normalization using reference genes is a common useful approach, but the validation of suitable reference genes remains...

arrayMagic: two-colour cDNA microarray quality control and preprocessing (2004)

Buneß, Andreas, Huber, Wolfgang, Steiner, Klaus, Sültmann, Holger, Poustka, Annemarie

Summary: arrayMagic is a software package for quality control and preprocessing of two-colour cDNA microarray data. The automated analysis pipeline comprises data import, normalisation, replica...

arrayMagic: two-colour cDNA microarray quality control and preprocessing (2004)

Buneß, Andreas, Huber, Wolfgang, Steiner, Klaus, Sültmann, Holger, Poustka, Annemarie

Summary: arrayMagic is a software package for quality control and preprocessing of two-colour cDNA microarray data. The automated analysis pipeline comprises data import, normalisation, replica...

Parameter estimation for the calibration and variance stabilization of microarray data (2003)

Huber,Wolfgang, Von Heydebreck,Anja, Sültmann,Holger, Poustka,Annemarie, Vingron,Martin

We derive and validate an estimator for the parameters of a transformation for the joint calibration (normalization) and variance stabilization of microarray intensity data. With this, the variances...

Parameter estimation for the calibration and variance stabilization of microarray data (2003)

Huber, Wolfgang, Von Heydebreck, Anja, Sültmann, Holger, Poustka, Annemarie, Vingron, Martin

We derive and validate an estimator for the parameters of a transformation for the joint calibration (normalization) and variance stabilization of microarray intensity data. With this, the variances...

Variance stabilization applied to microarray data calibration and to the quantification of differential expression (2002)

Huber,Wolfgang, Von Heydebreck,Anja, Sültmann,Holger, Poustka,Annemarie, Vingron,Martin

We introduce a statistical model for microarray gene expression data that comprises data calibration, the quantification of differential expression, and the quantification of measurement error. In...

Variance stabilization applied to microarray data calibration and to the quantification of differential expression (2002)

Huber, Wolfgang, Von Heydebreck, Anja, Sültmann, Holger, Poustka, Annemarie, Vingron, Martin

We introduce a statistical model for microarray gene expression data that comprises data calibration, the quantification of differential expression, and the quantification of measurement error. In...

Variance stabilization applied to microarray data calibration and to the quantification of differential expression (2002)

Huber, Wolfgang, Von Heydebreck, Anja, Sültmann, Holger, Poustka, Annemarie, Vingron, Martin

We introduce a statistical model for microarray gene expression data that comprises data calibration, the quantification of differential expression, and the quantification of measurement error. In...

Identification and Classification of Differentially Expressed Genes in Renal Cell Carcinoma by Expression Profiling on a Global Human 31,500-Element cDNA Array (2001)

Boer, Judith M., Huber, Wolfgang K., Sültmann, Holger, Wilmer, Friederike, Von Heydebreck, Anja, Haas, Stefan, ...

Identification and Classification of Differentially Expressed Genes in Renal Cell Carcinoma by Expression Profiling on a Global Human 31,500-Element cDNA Array (2001)

Boer, Judith M., Huber, Wolfgang K., Sültmann, Holger, Wilmer, Friederike, Von Heydebreck, Anja, Haas, Stefan, ...

Identification and Classification of Differentially Expressed Genes in Renal Cell Carcinoma by Expression Profiling on a Global Human 31,500-Element cDNA Array

Boer, Judith M., Huber, Wolfgang K., Sültmann, Holger, Wilmer, Friederike, Von Heydebreck, Anja, Haas, Stefan, ...

We investigated the changes in gene expression accompanying the development and progression of kidney cancer by use of 31,500-element complementary DNA arrays. We measured expression profiles for...

Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments

Schneider, Jörg, Buneß, Andreas, Huber, Wolfgang, Volz, Joachim, Kioschis, Petra, Hafner, Mathias, ...

From ORFeome to Biology: A Functional Genomics Pipeline

Wiemann, Stefan, Arlt, Dorit, Huber, Wolfgang, Wellenreuther, Ruth, Schleeger, Simone, Mehrle, Alexander, ...

As several model genomes have been sequenced, the elucidation of protein function is the next challenge toward the understanding of biological processes in health and disease. We have generated a...

Identification and Classification of Differentially Expressed Genes in Renal Cell Carcinoma by Expression Profiling on a Global Human 31,500-Element cDNA Array

Boer, Judith M., Huber, Wolfgang K., Sültmann, Holger, Wilmer, Friederike, Von Heydebreck, Anja, Haas, Stefan, ...

We investigated the changes in gene expression accompanying the development and progression of kidney cancer by use of 31,500-element complementary DNA arrays. We measured expression profiles for...

Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments

Schneider, Jörg, Buneß, Andreas, Huber, Wolfgang, Volz, Joachim, Kioschis, Petra, Hafner, Mathias, ...

From ORFeome to Biology: A Functional Genomics Pipeline

Wiemann, Stefan, Arlt, Dorit, Huber, Wolfgang, Wellenreuther, Ruth, Schleeger, Simone, Mehrle, Alexander, ...

As several model genomes have been sequenced, the elucidation of protein function is the next challenge toward the understanding of biological processes in health and disease. We have generated a...

Combinatorial RNAi for quantitative protein network analysis

Sahin, Özgür, Löbke, Christian, Korf, Ulrike, Appelhans, Heribert, Sültmann, Holger, Poustka, Annemarie, ...

The elucidation of cross-talk events between intersecting signaling pathways is one main challenge in biological research. The complexity of protein networks, composed of different pathways, requires...

Predicting pathway membership via domain signatures

Fröhlich, Holger, Fellmann, Mark, Sültmann, Holger, Poustka, Annemarie, Beißbarth, Tim

Motivation: Functional characterization of genes is of great importance for the understanding of complex cellular processes. Valuable information for this purpose can be obtained from pathway...

Estimating large-scale signaling networks through nested effect models with intervention effects from microarray data

Fröhlich, Holger, Fellmann, Mark, Sültmann, Holger, Poustka, Annemarie, Beissbarth, Tim

Motivation: Targeted interventions using RNA interference in combination with the measurement of secondary effects with DNA microarrays can be used to computationally reverse engineer features of...