Hyun S. Lillehoj

Salmonella Enteritidis–Induced Alteration of Inflammatory CXCL Chemokine Messenger-RNA Expression and Histologic Changes in the Ceca of Infected Chicks (2008)

Jennifer H. Cheeseman, Nyssa A. Levy, Pete Kaiser, Hyun S. Lillehoj, Susan J. Lamont

To understand better the events in early avian host immune responses to Salmonella Enteritidis (SE), we examined messenger-RNA (mRNA) expression for eight genes: CXCLi1[K60], CXCLi2 [IL-8/CAF],...

Antimicrobial Activity of Chicken NK-Lysin Against Eimeria Sporozoites (2008)

Yeong H. Hong, Hyun S. Lillehoj, Gregory R. Siragusa, Douglas D. Bannerman, Erik P. Lillehoj

NK-lysin is an antimicrobial and antitumor polypeptide that is considered to play an important role in innate immunity. Chicken NK-lysin is a member of the saposin-like protein family and exhibits...

Immunopathology and Cytokine Responses in Broiler Chickens Coinfected with Eimeria maxima and Clostridium perfringens with the Use of an Animal Model of Necrotic Enteritis (2008)

Soon S. Park, Hyun S. Lillehoj, Patricia C. Allen, Dong Woon Park, Steve FitzCoy, Daniel A. Bautista, ...

The incidence of necrotic enteritis (NE) due to Clostridium perfringens (CP) infection in commercial poultry has been increasing at an alarming rate. Although pre-exposure of chickens to coccidia...

EMBRYO VACCINATION AGAINST EIMERIA TENELLA AND E. ACERVULINA INFECTIONS USING RECOMBINANT PROTEINS AND CYTOKINE ADJUVANTS (2005)

Hyun S. Lillehoj, Xicheng Ding, Rami A. Dalloul, Takanori Sato, Atsushi Yasuda, Erik P. Lillehoj

Avian coccidiosis is an intestinal disease caused by protozoa of the genus Eimeria. To investigate the potential of recombinant protein vaccines to control coccidiosis, we cloned 2 Eimeria sp. genes...

Recent Advances in Immunomodulation and Vaccination Strategies Against Coccidiosis (2005)

Rami A. Dalloul, Hyun S. Lillehoj

Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies rely heavily on...

Resistance to Intestinal Coccidiosis Following DNA Immunization with the Cloned 3-1E Eimeria Gene Plus IL-2, IL-15, and IFN-γ (2005)

Hyun S. Lillehoj, Xicheng Ding, Marco A. Quiroz, Erich Bevensee, Erik P. Lillehoj

A cloned Eimeria acervulina gene (3-1E) was used to vaccinate chickens in ovo against coccidiosis, both alone and in combination with genes encoding interleukin (IL)-1, IL-2, IL-6, IL-8, IL-15,...

In Vivo Effects of CpG Oligodeoxynucleotide on Eimeria Infection in Chickens (2004)

Rami A. Dalloul, Hyun S. Lillehoj, Masashi Okamura, Hang Xie, Wongi Min, Xicheng Ding, ...

Poultry coccidiosis is the major parasitic disease of poultry and, until now, no recombinant vaccine has been developed. Short oligodeoxynucleotides (ODNs) containing unmethylated CpG motifs (CpG...

Nitric Oxide Production by Macrophages Stimulated with Coccidia Sporozoites, Lipopolysaccharide, or Interferon-γ, and Its Dynamic Changes in SC and TK Strains of Chickens Infected with Eimeria tenella (2004)

Hyun S. Lillehoj, Guangxing Li

Nitric oxide (NO) is an important mediator of innate and acquired immunities. In the studies reported here, we quantified NO produced in vitro by chicken leukocytes and macrophages and in vivo during...

Intestinal Immunomodulation by Vitamin A Deficiency and Lactobacillus-Based Probiotic in Eimeria acervulina–Infected Broiler Chickens (2003)

Rami A. Dalloul, Hyun S. Lillehoj, Timothy A. Shellem, John A. Doerr

SUMMARY. In a 2 × 2 factorial study, a broiler starter ration was amended for vitamin A (control, C; deficient, A) and probiotic status (−, P) to investigate their modulatory effects on the host...

Lymphocyte Proliferation Response During Eimeria tenella Infection Assessed by a New, Reliable, Nonradioactive Colorimetric Assay (2002)

Tadashi Miyamoto, Wongi Min, Hyun S. Lillehoj

SUMMARY. The application of a tetrazolium salt, WST-8, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt to the lymphocyte proliferation assay in the...

Interleukin-2 Production in SC and TK Chickens Infected with Eimeria tenella (2002)

Guangxing Li, Erik P. Lillehoj, Hyun S. Lillehoj

SUMMARY. SC and TK inbred chicken strains display differential protective immunity to coccidiosis, SC being more resistant and TK susceptible to disease. In this study, the association between...

Eimeria tenella Infection Induces Local Gamma Interferon Production and Intestinal Lymphocyte Subpopulation Changes

Yun, Cheol H., Lillehoj, Hyun S., Choi, Kang D.

The role of intestinal lymphocytes and gamma interferon (IFN-γ) production in protective immunity to Eimeria tenella infection was evaluated in two inbred strains of chickens (SC and TK) that...

Protective Immunity against Eimeria acervulina following In Ovo Immunization with a Recombinant Subunit Vaccine and Cytokine Genes

Ding, Xicheng, Lillehoj, Hyun S., Quiroz, Marco A., Bevensee, Erich, Lillehoj, Erik P.

A purified recombinant protein from Eimeria acervulina (3-1E) was used to vaccinate chickens in ovo against coccidiosis both alone and in combination with expression plasmids encoding the interleukin...

Eimeria tenella Infection Induces Local Gamma Interferon Production and Intestinal Lymphocyte Subpopulation Changes

Yun, Cheol H., Lillehoj, Hyun S., Choi, Kang D.

The role of intestinal lymphocytes and gamma interferon (IFN-γ) production in protective immunity to Eimeria tenella infection was evaluated in two inbred strains of chickens (SC and TK) that...

Protective Immunity against Eimeria acervulina following In Ovo Immunization with a Recombinant Subunit Vaccine and Cytokine Genes

Ding, Xicheng, Lillehoj, Hyun S., Quiroz, Marco A., Bevensee, Erich, Lillehoj, Erik P.

A purified recombinant protein from Eimeria acervulina (3-1E) was used to vaccinate chickens in ovo against coccidiosis both alone and in combination with expression plasmids encoding the interleukin...

Humoral and cellular immune response to thyroglobulin in different inbred rat strains

Lillehoj, Hyun S., Rose, Noel R.

The role of humoral and cellular immunity in experimental autoimmune thyroiditis was examined in inbred rat strains. A significant relationship between thyroiditis and both in vitro proliferative...