Single-step selection of mammalian cell mutants deficient in CTP synthetase (1989)
Li, I-Chian, Wu, Chao-Liang, Chu, Ernest H. Y.
A single-step selection of Chinese hamster V79 cells deficient in CTP synthetase (CTPS − ) is presented. The underlying principle of the direct selection is the differential and efficient killing...
Li, I-Chian, Chu, Ernest H. Y.
A systematic comparison of 5 different statistical methods for the estimation of mutation rate ([mu]) in cultured Chinese hamster V79 cells is presented. Fluctuation tests were performed with several...
A deterministic approach for the estimation of mutation rates in cultured mammalian cells (1985)
Li, I-Chian, Ho Wu, Shu-Chen, Fu, Jiliang, Chu, Ernest H. Y.
Unequal growth rates between mutant and wild-type cells in a large population constitute a problem for the estimation of mutation rate. Over a period of cell growth, a selective advantage of one cell...
Pleiotropic mutants of Chinese hamster cells with altered cytidine 5′-triphosphate synthetase (1984)
Li, I-Chian, Chu, Ernest H. Y., McLaren, John D., Lamb, Barbara J.
Following chemical mutagenesis and multiple-step indirect selection, four clones of Chinese hamster V79 cells were isolated which exhibited auxotrophy for thymidine, deoxycytidine, or deoxyuridine...
Mutants of chinese hamster cells deficient in thymidylate synthetase (1984)
Li, I-Chian, Chu, Ernest H. Y.
Stable mutants of Chinese hamster V79 cells deficient in thymidylate synthetase (TS; E.C. 2.1.1.45) have been selected from cultures grown in medium supplemented with folinic acid, aminopterin, and...
Estimation of mutation rates in cultured mammalian cells (1983)
Li, I-Chian, Fu, Jiliang, Hung, Yung-Tai, Chu, Ernest H. Y.
The factors that affect reliable estimations of mutation rates ([mu]) in cultured mammalian somatic cell populations by fluctuation analysis are studied experimentally and statistically. We analyze...
Fu, Jiliang, Li, I-Chian, Chu, Ernest H. Y.
Peer Reviewed
Modification of cell membrane in variants of chinese hamster cells resistant to abrin (1980)
Li, I-Chian, Blake, Diane A., Goldstein, Irwin J., Chu, Ernest H. Y.
Stable and heritable variants of Chinese hamster ovary (CHO) cells which are resistant to different levels (0.1, 1.0 and 10 [mu]g/ml) of the toxin abrin have been isolated and characterized. The...