Pascucci, B, Maga, G, Hübscher, U, Bjoras, M, Seeberg, E, Hickson, I D, ...
In mammalian cells, repair of the most abundant endogenous premutagenic lesion in DNA, 7,8-dihydro-8-oxoguanine (8-oxoG), is initiated by the bifunctional DNA glycosylase OGG1. By using purified...
Pascucci, B., Maga, G., Hübscher, U., Bjoras, M., Seeberg, E., Hickson, I. D., ...
In mammalian cells, repair of the most abundant endogenous premutagenic lesion in DNA, 7,8-dihydro-8-oxoguanine (8-oxoG), is initiated by the bifunctional DNA glycosylase OGG1. By using purified...
Rothwell, D. G., Hang, B., Gorman, M. A., Freemont, P. S., Singer, B., Hickson, I. D.
HAP1, also known as APE/Ref-1, is the major apurinic/apyrimidinic (AP) endonuclease in human cells. Previous structural studies have suggested a possible role for the Asp-210 residue of HAP1 in the...
Pascucci, B., Maga, G., Hübscher, U., Bjoras, M., Seeberg, E., Hickson, I. D., ...
In mammalian cells, repair of the most abundant endogenous premutagenic lesion in DNA, 7,8-dihydro-8-oxoguanine (8-oxoG), is initiated by the bifunctional DNA glycosylase OGG1. By using purified...
Asparagine 212 is essential for abasic site recognition by the human DNA repair endonuclease HAP1.
HAP1 is a divalent cation-dependent endonuclease from human cells with specificity for apurinic/apyrimidinic (AP) sites in DNA. Extraction of the essential metal ion from purified HAP1 stabilized its...
p53 regulates the minimal promoter of the human topoisomerase IIalpha gene.
Sandri, M I, Isaacs, R J, Ongkeko, W M, Harris, A L, Hickson, I D, Broggini, M, ...
DNA topoisomerase IIalpha is an essential enzyme for chromosome segregation during mitosis. Consistent with a cell division-specific role, the expression of the topoisomerase IIalpha gene is strongly...
Wang, X, Watt, P M, Louis, E J, Borts, R H, Hickson, I D
Saccharomyces cerevisiae top2 mutants deficient in topoisomerase II activity are defective in chromosome segregation during both mitotic and meiotic cell divisions. To identify proteins that act in...
Topoisomerase III is essential for accurate nuclear division in Schizosaccharomyces pombe.
Goodwin, A, Wang, S W, Toda, T, Norbury, C, Hickson, I D
Topoisomerases catalyse changes in the topological state of DNA and are required for many aspects of DNA metabolism. While the functions of topoisomerases I and II in eukaryotes are well established,...
Lewis, A D, Hickson, I D, Robson, C N, Harris, A L, Hayes, J D, Griffiths, S A, ...
Glutathione-dependent enzymes play a central role in the protection of cells from cytotoxic chemicals and have been implicated in the intrinsic and acquired resistance of tumors to cytotoxic drugs....
Barzilay, G, Walker, L J, Robson, C N, Hickson, I D
HAP1 protein, the major apurinic/apyrimidinic (AP) endonuclease in human cells, is a member of a homologous family of multifunctional DNA repair enzymes including the Escherichia coli exonuclease III...
Human cells express two differentially spliced forms of topoisomerase II beta mRNA.
Davies, S L, Jenkins, J R, Hickson, I D
Screening of a human B-cell cDNA library with a topoisomerase II beta gene-specific probe revealed the presence of two distinct forms of topoisomerase II beta cDNA. One form (designated topoisomerase...
Finch, P W, Wilson, R E, Brown, K, Hickson, I D, Tomkinson, A E, Emmerson, P T
The nucleotide sequence of a 6,000 bp region of the E. coli chromosome that includes the 3' end of the coding region for the thyA gene and the entire recC gene has been determined. The proposed...
Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III.
Finch, P W, Wilson, R E, Brown, K, Hickson, I D, Emmerson, P T
The nucleotide sequence of a 3120 bp region of the E. coli chromosome that includes the entire ptr gene has been determined. The proposed coding region for Protease III is 2889 nucleotides long,...
Complete nucleotide sequence of the Escherichia coli recB gene.
Finch, P W, Storey, A, Chapman, K E, Brown, K, Hickson, I D, Emmerson, P T
The complete nucleotide sequence of the Escherichia coli recB gene which encodes a subunit of the ATP-dependent DNase, Exonuclease V, has been determined. The proposed coding region for the RecB...
Finch, P W, Storey, A, Brown, K, Hickson, I D, Emmerson, P T
Intracellular amplification of the Escherichia coli RecB and RecC proteins does not result in an increase in Exonuclease V activity unless the level of a third protein, encoded between the recB and...
Molecular amplification and purification of the E. coli recC gene product.
Hickson, I D, Atkinson, K E, Hutton, L, Tomkinson, A E, Emmerson, P T
The level of recC gene expression has been analysed using Mud(bla lac) fusions to the recC promoter. The constitutive level of expression is very low and remains so even under SOS inducing...
Apurinic/apyrimidinic (AP) sites in cellular DNA are considered to be both cytotoxic and mutagenic, and can arise spontaneously or following exposure to DNA damaging agents. We have isolated cDNA...
Van Assendelft, G B, Rigney, E M, Hickson, I D
Ultraviolet (UV) light induces a variety of lesions in DNA of which the pyrimidine dimer represents the major species. Pyrimidine dimers exist as both a cyclobutane type and a 6-4'...
Davies, S M, Harris, A L, Hickson, I D
Ataxia telangiectasia (AT) cell lines are characterised by their hypersensitivity to ionizing radiation and bleomycin, and their failure to inhibit DNA synthesis after DNA damage. A recent report...
Robson, C N, Milne, A M, Pappin, D J, Hickson, I D
Ionizing radiation and radiomimetic compounds, such as hydrogen peroxide and bleomycin, generate DNA strand breaks with fragmented deoxyribose 3' termini via the formation of oxygen-derived free...
Structure of the human DNA repair gene HAP1 and its localisation to chromosome 14q 11.2-12.
Robson, C N, Hochhauser, D, Craig, R, Rack, K, Buckle, V J, Hickson, I D
Apurinic/apyrimidinic (AP) sites are pre-mutagenic DNA lesions which occur spontaneously and following exposure of cells to ionising radiation or chemical mutagens. HAP1 (Human AP endonuclease 1),...
Jenkins, J R, Ayton, P, Jones, T, Davies, S L, Simmons, D L, Harris, A L, ...
Topoisomerases catalyse the interconversion of topological isomers of DNA and have key roles in nucleic acid metabolism. Human cells express two distinct type II topoisomerase isozymes, designated...
Papathanasiou, M A, Kerr, N C, Robbins, J H, McBride, O W, Alamo, I, Barrett, S F, ...
The effect of ionizing radiation on the expression of two DNA-damage-inducible genes, designated gadd45 and gadd153, was examined in cultured human cells. These genes have previously been shown to be...
Walker, L J, Robson, C N, Black, E, Gillespie, D, Hickson, I D
The DNA binding activity of the c-jun proto-oncogene product is inhibited by oxidation of a specific cysteine residue (Cys-252) in the DNA binding domain. Jun protein inactivated by oxidation of this...
Dixon, J, Kaklamanis, L, Turley, H, Hickson, I D, Leek, R D, Harris, A L, ...
AIMS--To provide a detailed knowledge of the distribution of the CD13 molecule, also known as the protease aminopeptidase-N, on both normal tissues and malignant neoplasms of epithelial and lymphoid...
Walker, L J, Craig, R B, Harris, A L, Hickson, I D
The HAP1 protein (also known as APE/Ref-1) is a bifunctional human nuclear enzyme required for repair of apurinic/apyrimidinic sites in DNA and reactivation of oxidized proto-oncogene products. To...
Gorman, M A, Morera, S, Rothwell, D G, Mol, C D, Tainer, J A, ...
The structure of the major human apurinic/ apyrimidinic endonuclease (HAP1) has been solved at 2.2 A resolution. The enzyme consists of two symmetrically related domains of similar topology and has...
Aoyama, M, Grabowski, D R, Dubyak, G R, Constantinou, A I, Rybicki, L A, Bukowski, R M, ...
Topoisomerase II (topo II), an essential enzyme for cell viability, is also the target for clinically important anti-neoplastic agents that stimulate topo II-mediated DNA scission. The role of...
Involvement of recB and recC genes of Escherichia coli in precise transposon excision.
The mutations texA343 and texA344, which increase transposon excision, are complemented by multicopy plasmids carrying recC+ and recB+, respectively, but not by derivative plasmids in which the recC...
Watt, P. M., Hickson, I. D., Borts, R. H., Louis, E. J.
The Saccharomyces cerevisiae SGS1 gene is homologous to Escherichia coli RecQ and the human BLM and WRN proteins that are defective in the cancer-prone disorder Bloom's syndrome and the premature...
Rothwell, D. G., Hang, B., Gorman, M. A., Freemont, P. S., Singer, B., Hickson, I. D.
HAP1, also known as APE/Ref-1, is the major apurinic/apyrimidinic (AP) endonuclease in human cells. Previous structural studies have suggested a possible role for the Asp-210 residue of HAP1 in the...
Pascucci, B., Maga, G., Hübscher, U., Bjoras, M., Seeberg, E., Hickson, I. D., ...
In mammalian cells, repair of the most abundant endogenous premutagenic lesion in DNA, 7,8-dihydro-8-oxoguanine (8-oxoG), is initiated by the bifunctional DNA glycosylase OGG1. By using purified...
Asparagine 212 is essential for abasic site recognition by the human DNA repair endonuclease HAP1.
HAP1 is a divalent cation-dependent endonuclease from human cells with specificity for apurinic/apyrimidinic (AP) sites in DNA. Extraction of the essential metal ion from purified HAP1 stabilized its...
p53 regulates the minimal promoter of the human topoisomerase IIalpha gene.
Sandri, M I, Isaacs, R J, Ongkeko, W M, Harris, A L, Hickson, I D, Broggini, M, ...
DNA topoisomerase IIalpha is an essential enzyme for chromosome segregation during mitosis. Consistent with a cell division-specific role, the expression of the topoisomerase IIalpha gene is strongly...
Wang, X, Watt, P M, Louis, E J, Borts, R H, Hickson, I D
Saccharomyces cerevisiae top2 mutants deficient in topoisomerase II activity are defective in chromosome segregation during both mitotic and meiotic cell divisions. To identify proteins that act in...
Topoisomerase III is essential for accurate nuclear division in Schizosaccharomyces pombe.
Goodwin, A, Wang, S W, Toda, T, Norbury, C, Hickson, I D
Topoisomerases catalyse changes in the topological state of DNA and are required for many aspects of DNA metabolism. While the functions of topoisomerases I and II in eukaryotes are well established,...
Involvement of recB and recC genes of Escherichia coli in precise transposon excision.
The mutations texA343 and texA344, which increase transposon excision, are complemented by multicopy plasmids carrying recC+ and recB+, respectively, but not by derivative plasmids in which the recC...
Lewis, A D, Hickson, I D, Robson, C N, Harris, A L, Hayes, J D, Griffiths, S A, ...
Glutathione-dependent enzymes play a central role in the protection of cells from cytotoxic chemicals and have been implicated in the intrinsic and acquired resistance of tumors to cytotoxic drugs....
Barzilay, G, Walker, L J, Robson, C N, Hickson, I D
HAP1 protein, the major apurinic/apyrimidinic (AP) endonuclease in human cells, is a member of a homologous family of multifunctional DNA repair enzymes including the Escherichia coli exonuclease III...
Human cells express two differentially spliced forms of topoisomerase II beta mRNA.
Davies, S L, Jenkins, J R, Hickson, I D
Screening of a human B-cell cDNA library with a topoisomerase II beta gene-specific probe revealed the presence of two distinct forms of topoisomerase II beta cDNA. One form (designated topoisomerase...
Finch, P W, Wilson, R E, Brown, K, Hickson, I D, Tomkinson, A E, Emmerson, P T
The nucleotide sequence of a 6,000 bp region of the E. coli chromosome that includes the 3' end of the coding region for the thyA gene and the entire recC gene has been determined. The proposed...
Complete nucleotide sequence of the Escherichia coli ptr gene encoding protease III.
Finch, P W, Wilson, R E, Brown, K, Hickson, I D, Emmerson, P T
The nucleotide sequence of a 3120 bp region of the E. coli chromosome that includes the entire ptr gene has been determined. The proposed coding region for Protease III is 2889 nucleotides long,...
Complete nucleotide sequence of the Escherichia coli recB gene.
Finch, P W, Storey, A, Chapman, K E, Brown, K, Hickson, I D, Emmerson, P T
The complete nucleotide sequence of the Escherichia coli recB gene which encodes a subunit of the ATP-dependent DNase, Exonuclease V, has been determined. The proposed coding region for the RecB...
Finch, P W, Storey, A, Brown, K, Hickson, I D, Emmerson, P T
Intracellular amplification of the Escherichia coli RecB and RecC proteins does not result in an increase in Exonuclease V activity unless the level of a third protein, encoded between the recB and...
Molecular amplification and purification of the E. coli recC gene product.
Hickson, I D, Atkinson, K E, Hutton, L, Tomkinson, A E, Emmerson, P T
The level of recC gene expression has been analysed using Mud(bla lac) fusions to the recC promoter. The constitutive level of expression is very low and remains so even under SOS inducing...
Apurinic/apyrimidinic (AP) sites in cellular DNA are considered to be both cytotoxic and mutagenic, and can arise spontaneously or following exposure to DNA damaging agents. We have isolated cDNA...
Van Assendelft, G B, Rigney, E M, Hickson, I D
Ultraviolet (UV) light induces a variety of lesions in DNA of which the pyrimidine dimer represents the major species. Pyrimidine dimers exist as both a cyclobutane type and a 6-4'...
Davies, S M, Harris, A L, Hickson, I D
Ataxia telangiectasia (AT) cell lines are characterised by their hypersensitivity to ionizing radiation and bleomycin, and their failure to inhibit DNA synthesis after DNA damage. A recent report...
Robson, C N, Milne, A M, Pappin, D J, Hickson, I D
Ionizing radiation and radiomimetic compounds, such as hydrogen peroxide and bleomycin, generate DNA strand breaks with fragmented deoxyribose 3' termini via the formation of oxygen-derived free...
Structure of the human DNA repair gene HAP1 and its localisation to chromosome 14q 11.2-12.
Robson, C N, Hochhauser, D, Craig, R, Rack, K, Buckle, V J, Hickson, I D
Apurinic/apyrimidinic (AP) sites are pre-mutagenic DNA lesions which occur spontaneously and following exposure of cells to ionising radiation or chemical mutagens. HAP1 (Human AP endonuclease 1),...
Jenkins, J R, Ayton, P, Jones, T, Davies, S L, Simmons, D L, Harris, A L, ...
Topoisomerases catalyse the interconversion of topological isomers of DNA and have key roles in nucleic acid metabolism. Human cells express two distinct type II topoisomerase isozymes, designated...
Papathanasiou, M A, Kerr, N C, Robbins, J H, McBride, O W, Alamo, I, Barrett, S F, ...
The effect of ionizing radiation on the expression of two DNA-damage-inducible genes, designated gadd45 and gadd153, was examined in cultured human cells. These genes have previously been shown to be...
Walker, L J, Robson, C N, Black, E, Gillespie, D, Hickson, I D
The DNA binding activity of the c-jun proto-oncogene product is inhibited by oxidation of a specific cysteine residue (Cys-252) in the DNA binding domain. Jun protein inactivated by oxidation of this...
Dixon, J, Kaklamanis, L, Turley, H, Hickson, I D, Leek, R D, Harris, A L, ...
AIMS--To provide a detailed knowledge of the distribution of the CD13 molecule, also known as the protease aminopeptidase-N, on both normal tissues and malignant neoplasms of epithelial and lymphoid...
Walker, L J, Craig, R B, Harris, A L, Hickson, I D
The HAP1 protein (also known as APE/Ref-1) is a bifunctional human nuclear enzyme required for repair of apurinic/apyrimidinic sites in DNA and reactivation of oxidized proto-oncogene products. To...
Gorman, M A, Morera, S, Rothwell, D G, Mol, C D, Tainer, J A, ...
The structure of the major human apurinic/ apyrimidinic endonuclease (HAP1) has been solved at 2.2 A resolution. The enzyme consists of two symmetrically related domains of similar topology and has...
Watt, P. M., Hickson, I. D., Borts, R. H., Louis, E. J.
The Saccharomyces cerevisiae SGS1 gene is homologous to Escherichia coli RecQ and the human BLM and WRN proteins that are defective in the cancer-prone disorder Bloom's syndrome and the premature...
Aoyama, M, Grabowski, D R, Dubyak, G R, Constantinou, A I, Rybicki, L A, Bukowski, R M, ...
Topoisomerase II (topo II), an essential enzyme for cell viability, is also the target for clinically important anti-neoplastic agents that stimulate topo II-mediated DNA scission. The role of...
Mapping of topoisomerase II α epitopes recognized by autoantibodies in idiopathic pulmonary fibrosis
GRIGOLO, B, MAZZETTI, I, BORZÌ, R M, HICKSON, I D, FABBRI, M, FASANO, L, ...
Autoantibodies against DNA topoisomerase II α have been identified in the sera of patients with idiopathic pulmonary fibrosis (IPF). To map topoisomerase II autoepitopes, we tested by ELISA and...