Perera, V, Nachamkin, I, Ung, H, Patterson, J, McConville, M, Coloe, P, ...
Campylobacter jejuni is recognized as the most common identifiable pathogen associated with the development of Guillain-Barre syndrome (GBS), an acute autoimmune-mediated disease affecting the...
Blocking antibody assay for confirmation of urogenital Chlamydia infection.
Nachamkin, I, Riddle, D L, O'Connor, J
We evaluated the Syva MicroTrak Chlamydia Blocking Antibody Assay as a confirmatory assay for the detection of Chlamydia trachomatis in urogenital specimens. During a 5-month period, 109 positives...
Utilization of tests for Lyme disease antibody at a university hospital.
Nachamkin, I, Riddle, D L, Feldman, M, Edelstein, P H
We performed a retrospective study on patients who had a positive screening antibody test result for antibody to Borrelia burgdorferi to determine the clinical indicators used by physicians to order...
Nachamkin, I, Yang, X H, Stern, N J
Campylobacter jejuni, an important cause of human gastrointestinal infection, is a major food-borne pathogen in the United States and worldwide. Since poultry becomes colonized and/or contaminated...
Rhoads, S, Marinelli, L, Imperatrice, C A, Nachamkin, I
In a prospective side-by-side comparison conducted from September through November 1994, we compared the MicroScan WalkAway system, a conventional biochemical identification system (Dade MicroScan,...
Analysis of HL and O serotypes of Campylobacter strains by the flagellin gene typing system.
Nachamkin, I, Ung, H, Patton, C M
We recently developed a molecular typing system for Campylobacter jejuni and Campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaA (I.Nachamkin, K....
Infection of adult Syrian hamsters with flagellar variants of Campylobacter jejuni.
Aguero-Rosenfeld, M E, Yang, X H, Nachamkin, I
Two variants of Campylobacter jejuni IN1 differing in the expression of flagella, IN1 (Fla+ Mot+, wild type) and IN1-NM (Fla- Mot-), were tested for their ability to establish infection in adult...
Common and specific epitopes of Campylobacter flagellin recognized by monoclonal antibodies.
Murine monoclonal antibodies to Campylobacter jejuni recognized a flagellin epitope common to most Campylobacter species and an epitope restricted to C. jejuni and C. coli. These epitopes are...
Imperatrice, C A, Nachamkin, I
We evaluated the Vitek EPS card as a screen for the enteric pathogens Salmonella spp., Shigella spp., and Yersinia enterocolitica. Salmonella spp., Shigella spp., and Y. enterocolitica (125, 54, and...
Black, W J, Schwalbe, R S, Nachamkin, I, Cannon, J G
The protein II (P.II) outer membrane proteins of Neisseria gonorrhoeae, which have been implicated in gonococcal pathogenesis, have been previously shown to undergo a type of phase variation in which...
Cannon, J G, Black, W J, Nachamkin, I, Stewart, P W
A hybridoma derived from a mouse immunized with gonococcal outer membranes produced an antibody, designated H.8, that bound to all strains of Neisseria gonorrhoeae and N. meningitidis tested, and to...
Sugasawara, R J, Cannon, J G, Black, W J, Nachamkin, I, Sweet, R L, Brooks, G F
This study showed that a protein II (PII) of Neisseria gonorrhoeae FA1090 appeared to act as a mediator of attachment to HeLa cells. Two colony variants of FA1090 were selected. Both gonococcal...
Intestinal immunoglobulin A (IgA) anti-campylobacter flagellin antibodies were measured by an enzyme-linked immunosorbent assay in patients with and without campylobacter infections. The level of...
Niacin-positive Mycobacterium kansasii isolated from immunocompromised patients.
Nachamkin, I, MacGregor, R R, Staneck, J L, Tsang, A Y, Denner, J C, Willner, M, ...
Niacin-positive Mycobacterium kansasii was isolated from three patients, two with respiratory infections and one with a perirectal abscess. The isolates were phenotypically similar to other strains...
Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis.
Nachamkin, I, Bohachick, K, Patton, C M
We developed and studied a molecular typing approach for Campylobacter spp. with restriction fragment length polymorphism (RFLP) analysis of the flagellin gene flaA in C. jejuni. Using polymerase...
Nachamkin, I, Axelrod, P, Talbot, G H, Fischer, S H, Wennersten, C B, Moellering, R C, ...
Enterococci isolated from different body sites were tested for high-level gentamicin resistance. A total of 259 enterococcal isolates were screened for resistance (MIC, greater than 2,000...
Rappaport, T, Sawyer, K P, Nachamkin, I
To develop laboratory methods for the rapid identification of gram-positive cocci from blood cultures, several commercial immunological and biochemical tests for identifying staphylococci and...
Comparison of three rapid methods for detection of antibodies to streptolysin O and DNase B.
Hostetler, C L, Sawyer, K P, Nachamkin, I
Three commercial systems were compared for ability to detect antibodies to streptolysin O (ASO) and DNase B (ADB). Streptozyme (Wampole Laboratories, Cranbury, N.J.) exhibited high sensitivity (100%)...
We evaluated the Bactigen Salmonella-Shigella Latex Agglutination Slide Test (Wampole Laboratories, Div. Carter-Wallace, Inc., Cranbury, N.J.) for detection of Salmonella and Shigella spp. in...
We measured isotype-specific human antibodies directed against Campylobacter jejuni native flagellin and a synthetic peptide derived from the N-terminal amino acid sequence of the protein by using a...
Harris, M C, Deuber, C, Polin, R A, Nachamkin, I
In our nursery, we identified neonates with positive urine latex particle agglutination (LPA) tests for group B streptococcus (GBS) antigen who did not have corroborating cultural evidence of...
Culture confirmation of Campylobacter spp. by latex agglutination.
A commercial latex agglutination test [Meritec-Campy (jcl), Meridian Diagnostics, Cincinnati, Ohio] was evaluated for identification of Campylobacter jejuni, C. coli, C. laridis, and other...
DNA probe culture confirmation assay for identification of thermophilic Campylobacter species.
Tenover, F C, Carlson, L, Barbagallo, S, Nachamkin, I
We studied the ability of a new DNA probe-based assay system to correctly identify isolates of the thermophilic campylobacters Campylobacter jejuni, C. coli, and C. laridis grown in vitro. We...
Evaluation of a commercial cytotoxicity assay for detection of Clostridium difficile toxin.
Nachamkin, I, Lotz-Nolan, L, Skalina, D
A comparative study was performed to determine the accuracy of Clostridium difficile toxin detection. A commercial cytotoxicity assay (Bartels Immunodiagnostic Supply, Bellevue, Wash.) was compared...
Nachamkin, I, Sawyer, K, Skalina, D, Crooks, G W, Ciotti, R, Sondheimer, S J
The direct immunofluorescence assay (DFA) was compared with culture for test-of-cure analysis for Chlamydia trachomatis in patients 7 to 10 days after antimicrobial therapy was given. DFA...
Western blot analysis was used to identify antigenic components of Campylobacter jejuni whole cells and outer membranes that elicit antibody responses in patients with campylobacter enteritis. Acute-...
Evaluation of a rapid system for species identification of alpha-hemolytic streptococci.
Nachamkin, I, Lynch, J R, Dalton, H P
A new 4-h commercial system (API 20S; Analytab Products, Plainview, N.Y.) for the identification of streptococci was compared with the conventional biochemical profile method in the species...
Clark, R B, Nachamkin, I, Schatzki, P F, Dalton, H P
Monoclonal antibodies were produced against the HAR-13 strain (A) of Chlamydia trachomatis. By an indirect immunofluorescence technique, three clones (1-7, 2-8, 9F) were found to produce antibody...
Nachamkin, I, Cannon, J G, Mittler, R S
Hybridomas secreting monoclonal antibodies against an apparent strain-specific cell surface antigen of Neisseria gonorrhoeae were produced. Spleen cells from BALB/c mice immunized with whole...
Blocking antibody assay for confirmation of urogenital Chlamydia infection.
Nachamkin, I, Riddle, D L, O'Connor, J
We evaluated the Syva MicroTrak Chlamydia Blocking Antibody Assay as a confirmatory assay for the detection of Chlamydia trachomatis in urogenital specimens. During a 5-month period, 109 positives...
Utilization of tests for Lyme disease antibody at a university hospital.
Nachamkin, I, Riddle, D L, Feldman, M, Edelstein, P H
We performed a retrospective study on patients who had a positive screening antibody test result for antibody to Borrelia burgdorferi to determine the clinical indicators used by physicians to order...
Nachamkin, I, Yang, X H, Stern, N J
Campylobacter jejuni, an important cause of human gastrointestinal infection, is a major food-borne pathogen in the United States and worldwide. Since poultry becomes colonized and/or contaminated...
Rhoads, S, Marinelli, L, Imperatrice, C A, Nachamkin, I
In a prospective side-by-side comparison conducted from September through November 1994, we compared the MicroScan WalkAway system, a conventional biochemical identification system (Dade MicroScan,...
Analysis of HL and O serotypes of Campylobacter strains by the flagellin gene typing system.
Nachamkin, I, Ung, H, Patton, C M
We recently developed a molecular typing system for Campylobacter jejuni and Campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaA (I.Nachamkin, K....
Infection of adult Syrian hamsters with flagellar variants of Campylobacter jejuni.
Aguero-Rosenfeld, M E, Yang, X H, Nachamkin, I
Two variants of Campylobacter jejuni IN1 differing in the expression of flagella, IN1 (Fla+ Mot+, wild type) and IN1-NM (Fla- Mot-), were tested for their ability to establish infection in adult...
Common and specific epitopes of Campylobacter flagellin recognized by monoclonal antibodies.
Murine monoclonal antibodies to Campylobacter jejuni recognized a flagellin epitope common to most Campylobacter species and an epitope restricted to C. jejuni and C. coli. These epitopes are...
Imperatrice, C A, Nachamkin, I
We evaluated the Vitek EPS card as a screen for the enteric pathogens Salmonella spp., Shigella spp., and Yersinia enterocolitica. Salmonella spp., Shigella spp., and Y. enterocolitica (125, 54, and...
Black, W J, Schwalbe, R S, Nachamkin, I, Cannon, J G
The protein II (P.II) outer membrane proteins of Neisseria gonorrhoeae, which have been implicated in gonococcal pathogenesis, have been previously shown to undergo a type of phase variation in which...
Cannon, J G, Black, W J, Nachamkin, I, Stewart, P W
A hybridoma derived from a mouse immunized with gonococcal outer membranes produced an antibody, designated H.8, that bound to all strains of Neisseria gonorrhoeae and N. meningitidis tested, and to...
Sugasawara, R J, Cannon, J G, Black, W J, Nachamkin, I, Sweet, R L, Brooks, G F
This study showed that a protein II (PII) of Neisseria gonorrhoeae FA1090 appeared to act as a mediator of attachment to HeLa cells. Two colony variants of FA1090 were selected. Both gonococcal...
Intestinal immunoglobulin A (IgA) anti-campylobacter flagellin antibodies were measured by an enzyme-linked immunosorbent assay in patients with and without campylobacter infections. The level of...
Niacin-positive Mycobacterium kansasii isolated from immunocompromised patients.
Nachamkin, I, MacGregor, R R, Staneck, J L, Tsang, A Y, Denner, J C, Willner, M, ...
Niacin-positive Mycobacterium kansasii was isolated from three patients, two with respiratory infections and one with a perirectal abscess. The isolates were phenotypically similar to other strains...
Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis.
Nachamkin, I, Bohachick, K, Patton, C M
We developed and studied a molecular typing approach for Campylobacter spp. with restriction fragment length polymorphism (RFLP) analysis of the flagellin gene flaA in C. jejuni. Using polymerase...
Nachamkin, I, Axelrod, P, Talbot, G H, Fischer, S H, Wennersten, C B, Moellering, R C, ...
Enterococci isolated from different body sites were tested for high-level gentamicin resistance. A total of 259 enterococcal isolates were screened for resistance (MIC, greater than 2,000...
Rappaport, T, Sawyer, K P, Nachamkin, I
To develop laboratory methods for the rapid identification of gram-positive cocci from blood cultures, several commercial immunological and biochemical tests for identifying staphylococci and...
Comparison of three rapid methods for detection of antibodies to streptolysin O and DNase B.
Hostetler, C L, Sawyer, K P, Nachamkin, I
Three commercial systems were compared for ability to detect antibodies to streptolysin O (ASO) and DNase B (ADB). Streptozyme (Wampole Laboratories, Cranbury, N.J.) exhibited high sensitivity (100%)...
We evaluated the Bactigen Salmonella-Shigella Latex Agglutination Slide Test (Wampole Laboratories, Div. Carter-Wallace, Inc., Cranbury, N.J.) for detection of Salmonella and Shigella spp. in...
We measured isotype-specific human antibodies directed against Campylobacter jejuni native flagellin and a synthetic peptide derived from the N-terminal amino acid sequence of the protein by using a...
Harris, M C, Deuber, C, Polin, R A, Nachamkin, I
In our nursery, we identified neonates with positive urine latex particle agglutination (LPA) tests for group B streptococcus (GBS) antigen who did not have corroborating cultural evidence of...
Culture confirmation of Campylobacter spp. by latex agglutination.
A commercial latex agglutination test [Meritec-Campy (jcl), Meridian Diagnostics, Cincinnati, Ohio] was evaluated for identification of Campylobacter jejuni, C. coli, C. laridis, and other...
DNA probe culture confirmation assay for identification of thermophilic Campylobacter species.
Tenover, F C, Carlson, L, Barbagallo, S, Nachamkin, I
We studied the ability of a new DNA probe-based assay system to correctly identify isolates of the thermophilic campylobacters Campylobacter jejuni, C. coli, and C. laridis grown in vitro. We...
Evaluation of a commercial cytotoxicity assay for detection of Clostridium difficile toxin.
Nachamkin, I, Lotz-Nolan, L, Skalina, D
A comparative study was performed to determine the accuracy of Clostridium difficile toxin detection. A commercial cytotoxicity assay (Bartels Immunodiagnostic Supply, Bellevue, Wash.) was compared...
Nachamkin, I, Sawyer, K, Skalina, D, Crooks, G W, Ciotti, R, Sondheimer, S J
The direct immunofluorescence assay (DFA) was compared with culture for test-of-cure analysis for Chlamydia trachomatis in patients 7 to 10 days after antimicrobial therapy was given. DFA...
Western blot analysis was used to identify antigenic components of Campylobacter jejuni whole cells and outer membranes that elicit antibody responses in patients with campylobacter enteritis. Acute-...
Evaluation of a rapid system for species identification of alpha-hemolytic streptococci.
Nachamkin, I, Lynch, J R, Dalton, H P
A new 4-h commercial system (API 20S; Analytab Products, Plainview, N.Y.) for the identification of streptococci was compared with the conventional biochemical profile method in the species...
Clark, R B, Nachamkin, I, Schatzki, P F, Dalton, H P
Monoclonal antibodies were produced against the HAR-13 strain (A) of Chlamydia trachomatis. By an indirect immunofluorescence technique, three clones (1-7, 2-8, 9F) were found to produce antibody...
Nachamkin, I, Cannon, J G, Mittler, R S
Hybridomas secreting monoclonal antibodies against an apparent strain-specific cell surface antigen of Neisseria gonorrhoeae were produced. Spleen cells from BALB/c mice immunized with whole...
Detecting Chlamydia trachomatis by direct immunofluorescence using a Cytobrush sampling technique.
Ciotti, R A, Sondheimer, S J, Nachamkin, I
We compared two different methods of collecting endocervical samples for examination by direct immunofluorescence for Chlamydia trachomatis. A cervical Cytobrush gave better results than a dacron...
Engberg, J., Aarestrup, F. M., Taylor, D. E., Gerner-Smidt, P., Nachamkin, I.
The incidence of human Campylobacter jejuni and C. coli infections has increased markedly in many parts of the world in the last decade as has the number of quinolone-resistant and, to a lesser...