A DNA helicase induced by herpes simplex virus type 1 (1988)
Crute, James J., Mocarski, Edward S., Lehman, I.R.
We have identified and partially purified a DNA-dependent ATPase that is present specifically in herpes simplex virus type 1-infected Vero cells. The enzyme which has a molecular weight of...
Renaturation of DNA: a novel reaction of histones (1981)
Histones isolated from several sources, either singly or in combination promote the renaturation of complementary single strands of DNA, as measured by the acquisition of resistance to S1 nuclease....
Falkenberg, Maria, Lehman, I. R., Elias, Per
The synthesis of double-stranded DNA by a rolling circle mechanism was reconstituted in vitro with a replisome consisting of the DNA polymerase-UL42 complex and the heterotrimeric helicase-primase...
The herpes simplex virus type 1 (HSV-1) genome contains three origins of replication: oriL and two copies of oriS. These origins contain specific sequences, box I and box II, linked by an AT-rich...
Using a spectrophotometric assay that measures the hyperchromicity that accompanies the unwinding of a DNA duplex, we have identified an ATP-independent step in the unwinding of a herpes simplex...
Interaction of herpes simplex virus 1 origin-binding protein with DNA polymerase alpha.
Lee, S S, Dong, Q, Wang, T S, Lehman, I R
The herpes simplex virus 1 (HSV-1) genome encodes seven polypeptides that are required for its replication. These include a heterodimeric DNA polymerase, a single-strand-DNA-binding protein, a...
Rolling circle DNA replication in vitro by a complex of herpes simplex virus type 1-encoded enzymes.
Extracts of insect cells infected with baculoviruses recombinant for the herpes simplex virus 1 (HSV-1)-encoded enzymes that are required for its replication can promote the rolling circle...
We had previously demonstrated that the herpes simplex virus 1 (HSV-1) single-stranded DNA-binding protein (ICP8) can specifically stimulate the helicase activity of the HSV-1 origin-binding protein...
DNA polymerase delta from embryos of Drosophila melanogaster.
Chiang, C S, Mitsis, P G, Lehman, I R
We have purified a DNA polymerase activity from 0- to 2-hr embryos of Drosophila melanogaster to near homogeneity. The purified enzyme consists of a single 120-kDa polypeptide, which contains...
Recombination in vitro between herpes simplex virus type 1 a sequences.
Bruckner, R C, Dutch, R E, Zemelman, B V, Mocarski, E S, Lehman, I R
We have partially purified an activity from extracts of cells infected with herpes simplex virus type 1 that mediates recombination between repeated copies of the 317-base-pair a sequence of herpes...
Herpes simplex virus 1 encodes a helicase-primase that is composed of the products of the UL5, UL8, and UL52 genes. A stable subassembly consisting of only the UL5 and UL52 gene products has been...
A herpes simplex virus type 1 (HSV-1) OriS analogue in which the A+T sequence linking the box I and II elements was replaced by two single-stranded oligo(dT)s is unwound by the UL9 protein-ICP8...
Dutch, R E, Bianchi, V, Lehman, I R
Using an assay for recombination that measures deletion of a beta-galactosidase gene positioned between two directly repeated 350-bp sequences in plasmids transiently maintained in COS cells, we have...
Rolling circle DNA replication by extracts of herpes simplex virus type 1-infected human cells.
Skaliter, R, Makhov, A M, Griffith, J D, Lehman, I R
Whole-cell extracts of herpes simplex virus type 1-infected human cells (293 cells) can promote the rolling circle replication of circular duplex DNA molecules. The products of the reaction are...
Dutch, R E, Zemelman, B V, Lehman, I R
The a sequences of herpes simplex virus type 1 are believed to be the cis sites for inversion events that generate four isomeric forms of the viral genome. Using an assay that measures deletion of a...
Herpes simplex virus type 1 ICP8: helix-destabilizing properties.
The major single-stranded DNA-binding protein, ICP8, of herpes simplex virus type 1 (HSV-1) is one of seven virus-encoded polypeptides required for HSV-1 DNA replication. To investigate the role of...
Renaturation of complementary DNA strands by herpes simplex virus type 1 ICP8.
ICP8, the major single-stranded DNA-binding protein of herpes simplex virus type 1, promotes renaturation of complementary single strands of DNA. This reaction is ATP independent but requires Mg2+....
Herpes simplex virus type 1 recombination: role of DNA replication and viral a sequences.
Dutch, R E, Bruckner, R C, Mocarski, E S, Lehman, I R
During the course of infection, elements of the herpes simplex virus type 1 (HSV-1) genome undergo inversion, a process that is believed to occur through the viral a sequences. To investigate the...
Hernandez, T R, Dutch, R E, Lehman, I R, Gustafsson, C, Elias, P
The herpes simplex virus type 1 genome contains three origins of replication: OriL and a diploid OriS. The origin-binding protein, the product of the UL9 gene, interacts with two sites within OriS,...
Release of RNA polymerase from vero cell mitochondria after herpes simplex virus type 1 infection.
Infection of Vero cells with herpes simplex virus type 1 results in the appearance in soluble extracts of a DNA primase activity. The partially purified enzyme, Mr, approximately 100,000, is...
Interaction of origin binding protein with an origin of replication of herpes simplex virus 1.
Herpes simplex virus 1 encodes a protein that binds specifically to a viral DNA replication origin (oriS). This origin-binding protein has been purified to homogeneity by means of sequence-specific...
Herpes simplex virus 1 helicase-primase: a complex of three herpes-encoded gene products.
Crute, J J, Tsurumi, T, Zhu, L A, Weller, S K, Olivo, P D, Challberg, M D, ...
In an earlier report, we described a DNA helicase that is specifically induced upon infection of Vero cells with herpes simplex virus 1. We have purified this enzyme to near homogeneity and found it...
Cotterill, S M, Reyland, M E, Loeb, L A, Lehman, I R
The DNA polymerase-primase from Drosophila lacks 3'----5' exonuclease activity. However, a potent exonuclease can be detected after separating the 182-kDa polymerase subunit from the other three...
The recA protein of Escherichia coli promotes the complete exchange of strands between full-length linear duplex and single-stranded circular DNA molecules of bacteriophage phi X-174, converting more...
Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome.
Greenleaf, A L, Kelly, J L, Lehman, I R
A 4-kilobase DNA fragment carried by a recombinant lambda gt11 bacteriophage appears to contain most of the coding information for the 145-kDa subunit of the Saccharomyces cerevisiae mitochondrial...
Renaturation of DNA: a novel reaction of histones.
Histones isolated from several sources, either singly or in combination promote the renaturation of complementary single strands of DNA, as measured by the acquisition of resistance to S1 nuclease....
Cotterill, S, Lehman, I R, McLachlan, P
We have isolated both cDNA and genomic clones for the 73 kDa subunit of the DNA polymerase alpha primase of Drosophila melanogaster. Analysis of these clones has identified an open reading frame of...
A DNA helicase induced by herpes simplex virus type 1.
Crute, J J, Mocarski, E S, Lehman, I R
We have identified and partially purified a DNA-dependent ATPase that is present specifically in herpes simplex virus type 1-infected Vero cells. The enzyme which has a molecular weight of...
A DNA primase activity associated with DNA polymerase alpha from Drosophila melanogaster embryos.
Preparations of DNA polymerase alpha from early embryos of Drosophila melanogaster catalyze the ATP-dependent synthesis of DNA with single-stranded M13 DNA or poly(dT) templates. In the case of M13...
Recombinational bypass of pyrimidine dimers promoted by the recA protein of Escherichia coli.
recA protein, in the presence of single-stranded DNA binding protein and ATP, promotes the complete exchange of strands between circular single-stranded DNA containing pyrimidine dimers and a...
Synthesis by the DNA primase of Drosophila melanogaster of a primer with a unique chain length.
The primase associated with the DNA polymerase alpha from embryos of Drosophila melanogaster catalyzes the synthesis of ribo-oligonucleotide primers on single-stranded M13 DNA or polydeoxythymidylate...
McEntee, K, Weinstock, G M, Lehman, I R
The single-stranded DNA-binding protein of Escherichia coli significantly alters the strand assimilation reaction catalyzed by recA protein [McEntee, K., Weinstock, G. M. & Lehman, I. R. (1979) Proc....
Directionality and polarity in recA protein-promoted branch migration.
The recA protein of Escherichia coli promotes the complete exchange of strands between full-length linear duplex and single-stranded circular phi X174 DNA molecules. Analysis of the reaction by...
ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli.
Weinstock, G M, McEntee, K, Lehman, I R
The product of the recA gene of Escherichia coli has been purified to near-homogeneity by a simple three-step procedure. Incubation of the recA protein with complementary single strands of DNA, Mg2+,...
Initiation of general recombination catalyzed in vitro by the recA protein of Escherichia coli.
McEntee, K, Weinstock, G M, Lehman, I R
Homogeneous recA protein catalyzes the hybridization of single-stranded DNA to homologous regions in duplex DNA. The products are D-loops, which are formed with equal efficiency in linear and...
A DNA binding protein specific for an origin of replication of herpes simplex virus type 1.
Elias, P, O'Donnell, M E, Mocarski, E S, Lehman, I R
We have identified a protein that binds specifically to an origin of replication (oris) of the herpes simplex virus type 1 genome. The oris binding protein, detectable only in nuclear extracts of...
Konrad, E. Bruce, Lehman, I. R.
A mutant strain of E. coli, initially identified by an abnormally high frequency of recombination, has been found to be defective in the 5′ → 3′ exonuclease associated with DNA polymerase I,...
Enzymatic Characterization of a Mutant of Escherichia coli with an Altered DNA Ligase
A temperature-sensitive, radiation-sensitive mutant of Escherichia coli has been assayed for DNA ligase activity in vitro. The strain contains a markedly reduced amount of DNA-joining activity, which...
Gumport, Richard I., Lehman, I. R.
Proteolytic degradation of the Escherichia coli DNA ligase-adenylate intermediate releases adenosine 5′-monophosphate linked to the ε-amino group of lysine by a phosphoamide bond. Measurements of...
Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA.
Tye, B K, Nyman, P O, Lehman, I R, Hochhauser, S, Weiss, B
Strains of Escherichia coli with a mutation in the sof (dnaS) locus show a higher than normal frequency of recombination (are hyper rec) and incorporate label into short (4-5S) DNA fragments...
Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster.
Kaguni, L S, Rossignol, J M, Conaway, R C, Lehman, I R
A procedure has been devised for the purification of intact DNA polymerase alpha from early embryos of Drosophila melanogaster. The purified enzyme consists of at least three polypeptides with Mrs of...
The renaturation of complementary DNA strands by the recA protein of Escherichia coli has been found to exhibit the following features. (i) Optimal renaturation occurs at recA protein levels below...
Tye, Bik-Kwoon, Chien, Janice, Lehman, I. R., Duncan, Bruce K., Warner, Huber R.
Uracil is incorporated into newly synthesized DNA by mutants of Escherichia coli with reduced levels of dUTPase (dUTP nucleotidohydrolase; EC 3.6.1.23). Excision-repair of the incorporated uracil...
Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates.
A new group of mutants has been isolated which, during short pulses, incorporate (3-H)thymidine into DNA fragments that are substantially smaller than Okazaki fragments. These small fragments can be...
Cameron, J R, Panasenko, S M, Lehman, I R, Davis, R W
DNA from lambdagt-lambdaB bacteriophage was cleaved with EcoRI endonuclease and fragments from EcoRI-digested E. coli DNA were inserted. This DNA was used to infect E. coli, and phages containing the...
Makhov, A M, Boehmer, P E, Lehman, I R, Griffith, J D
The UL9 protein of herpes simplex virus type 1 (HSV-1) binds specifically to the HSV-1 oriS and oriL origins of replication, and is a DNA helicase and DNA-dependent NTPase. In this study electron...
Bessman, Maurice J., Lehman, I. R., Adler, Julius, Zimmerman, Steven B., Simms, E. S., Kornberg, Arthur
Lehman, I. R., Zimmerman, Steven B., Adler, Julius, Bessman, Maurice J., Simms, E. S., Kornberg, Arthur
Falkenberg, Maria, Lehman, I. R., Elias, Per
The synthesis of double-stranded DNA by a rolling circle mechanism was reconstituted in vitro with a replisome consisting of the DNA polymerase-UL42 complex and the heterotrimeric helicase-primase...
The herpes simplex virus type 1 (HSV-1) genome contains three origins of replication: oriL and two copies of oriS. These origins contain specific sequences, box I and box II, linked by an AT-rich...
Using a spectrophotometric assay that measures the hyperchromicity that accompanies the unwinding of a DNA duplex, we have identified an ATP-independent step in the unwinding of a herpes simplex...
Interaction of herpes simplex virus 1 origin-binding protein with DNA polymerase alpha.
Lee, S S, Dong, Q, Wang, T S, Lehman, I R
The herpes simplex virus 1 (HSV-1) genome encodes seven polypeptides that are required for its replication. These include a heterodimeric DNA polymerase, a single-strand-DNA-binding protein, a...
Rolling circle DNA replication in vitro by a complex of herpes simplex virus type 1-encoded enzymes.
Extracts of insect cells infected with baculoviruses recombinant for the herpes simplex virus 1 (HSV-1)-encoded enzymes that are required for its replication can promote the rolling circle...
We had previously demonstrated that the herpes simplex virus 1 (HSV-1) single-stranded DNA-binding protein (ICP8) can specifically stimulate the helicase activity of the HSV-1 origin-binding protein...
DNA polymerase delta from embryos of Drosophila melanogaster.
Chiang, C S, Mitsis, P G, Lehman, I R
We have purified a DNA polymerase activity from 0- to 2-hr embryos of Drosophila melanogaster to near homogeneity. The purified enzyme consists of a single 120-kDa polypeptide, which contains...
Recombination in vitro between herpes simplex virus type 1 a sequences.
Bruckner, R C, Dutch, R E, Zemelman, B V, Mocarski, E S, Lehman, I R
We have partially purified an activity from extracts of cells infected with herpes simplex virus type 1 that mediates recombination between repeated copies of the 317-base-pair a sequence of herpes...
Herpes simplex virus 1 encodes a helicase-primase that is composed of the products of the UL5, UL8, and UL52 genes. A stable subassembly consisting of only the UL5 and UL52 gene products has been...
A herpes simplex virus type 1 (HSV-1) OriS analogue in which the A+T sequence linking the box I and II elements was replaced by two single-stranded oligo(dT)s is unwound by the UL9 protein-ICP8...
Dutch, R E, Bianchi, V, Lehman, I R
Using an assay for recombination that measures deletion of a beta-galactosidase gene positioned between two directly repeated 350-bp sequences in plasmids transiently maintained in COS cells, we have...
Rolling circle DNA replication by extracts of herpes simplex virus type 1-infected human cells.
Skaliter, R, Makhov, A M, Griffith, J D, Lehman, I R
Whole-cell extracts of herpes simplex virus type 1-infected human cells (293 cells) can promote the rolling circle replication of circular duplex DNA molecules. The products of the reaction are...
Dutch, R E, Zemelman, B V, Lehman, I R
The a sequences of herpes simplex virus type 1 are believed to be the cis sites for inversion events that generate four isomeric forms of the viral genome. Using an assay that measures deletion of a...
Herpes simplex virus type 1 ICP8: helix-destabilizing properties.
The major single-stranded DNA-binding protein, ICP8, of herpes simplex virus type 1 (HSV-1) is one of seven virus-encoded polypeptides required for HSV-1 DNA replication. To investigate the role of...
Renaturation of complementary DNA strands by herpes simplex virus type 1 ICP8.
ICP8, the major single-stranded DNA-binding protein of herpes simplex virus type 1, promotes renaturation of complementary single strands of DNA. This reaction is ATP independent but requires Mg2+....
Herpes simplex virus type 1 recombination: role of DNA replication and viral a sequences.
Dutch, R E, Bruckner, R C, Mocarski, E S, Lehman, I R
During the course of infection, elements of the herpes simplex virus type 1 (HSV-1) genome undergo inversion, a process that is believed to occur through the viral a sequences. To investigate the...
Hernandez, T R, Dutch, R E, Lehman, I R, Gustafsson, C, Elias, P
The herpes simplex virus type 1 genome contains three origins of replication: OriL and a diploid OriS. The origin-binding protein, the product of the UL9 gene, interacts with two sites within OriS,...
Release of RNA polymerase from vero cell mitochondria after herpes simplex virus type 1 infection.
Infection of Vero cells with herpes simplex virus type 1 results in the appearance in soluble extracts of a DNA primase activity. The partially purified enzyme, Mr, approximately 100,000, is...
Interaction of origin binding protein with an origin of replication of herpes simplex virus 1.
Herpes simplex virus 1 encodes a protein that binds specifically to a viral DNA replication origin (oriS). This origin-binding protein has been purified to homogeneity by means of sequence-specific...
Herpes simplex virus 1 helicase-primase: a complex of three herpes-encoded gene products.
Crute, J J, Tsurumi, T, Zhu, L A, Weller, S K, Olivo, P D, Challberg, M D, ...
In an earlier report, we described a DNA helicase that is specifically induced upon infection of Vero cells with herpes simplex virus 1. We have purified this enzyme to near homogeneity and found it...
Cotterill, S M, Reyland, M E, Loeb, L A, Lehman, I R
The DNA polymerase-primase from Drosophila lacks 3'----5' exonuclease activity. However, a potent exonuclease can be detected after separating the 182-kDa polymerase subunit from the other three...
The recA protein of Escherichia coli promotes the complete exchange of strands between full-length linear duplex and single-stranded circular DNA molecules of bacteriophage phi X-174, converting more...
Yeast RPO41 gene product is required for transcription and maintenance of the mitochondrial genome.
Greenleaf, A L, Kelly, J L, Lehman, I R
A 4-kilobase DNA fragment carried by a recombinant lambda gt11 bacteriophage appears to contain most of the coding information for the 145-kDa subunit of the Saccharomyces cerevisiae mitochondrial...
Renaturation of DNA: a novel reaction of histones.
Histones isolated from several sources, either singly or in combination promote the renaturation of complementary single strands of DNA, as measured by the acquisition of resistance to S1 nuclease....
Cotterill, S, Lehman, I R, McLachlan, P
We have isolated both cDNA and genomic clones for the 73 kDa subunit of the DNA polymerase alpha primase of Drosophila melanogaster. Analysis of these clones has identified an open reading frame of...
A DNA helicase induced by herpes simplex virus type 1.
Crute, J J, Mocarski, E S, Lehman, I R
We have identified and partially purified a DNA-dependent ATPase that is present specifically in herpes simplex virus type 1-infected Vero cells. The enzyme which has a molecular weight of...
A DNA primase activity associated with DNA polymerase alpha from Drosophila melanogaster embryos.
Preparations of DNA polymerase alpha from early embryos of Drosophila melanogaster catalyze the ATP-dependent synthesis of DNA with single-stranded M13 DNA or poly(dT) templates. In the case of M13...
Recombinational bypass of pyrimidine dimers promoted by the recA protein of Escherichia coli.
recA protein, in the presence of single-stranded DNA binding protein and ATP, promotes the complete exchange of strands between circular single-stranded DNA containing pyrimidine dimers and a...
Synthesis by the DNA primase of Drosophila melanogaster of a primer with a unique chain length.
The primase associated with the DNA polymerase alpha from embryos of Drosophila melanogaster catalyzes the synthesis of ribo-oligonucleotide primers on single-stranded M13 DNA or polydeoxythymidylate...
McEntee, K, Weinstock, G M, Lehman, I R
The single-stranded DNA-binding protein of Escherichia coli significantly alters the strand assimilation reaction catalyzed by recA protein [McEntee, K., Weinstock, G. M. & Lehman, I. R. (1979) Proc....
Directionality and polarity in recA protein-promoted branch migration.
The recA protein of Escherichia coli promotes the complete exchange of strands between full-length linear duplex and single-stranded circular phi X174 DNA molecules. Analysis of the reaction by...
ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli.
Weinstock, G M, McEntee, K, Lehman, I R
The product of the recA gene of Escherichia coli has been purified to near-homogeneity by a simple three-step procedure. Incubation of the recA protein with complementary single strands of DNA, Mg2+,...
Initiation of general recombination catalyzed in vitro by the recA protein of Escherichia coli.
McEntee, K, Weinstock, G M, Lehman, I R
Homogeneous recA protein catalyzes the hybridization of single-stranded DNA to homologous regions in duplex DNA. The products are D-loops, which are formed with equal efficiency in linear and...
A DNA binding protein specific for an origin of replication of herpes simplex virus type 1.
Elias, P, O'Donnell, M E, Mocarski, E S, Lehman, I R
We have identified a protein that binds specifically to an origin of replication (oris) of the herpes simplex virus type 1 genome. The oris binding protein, detectable only in nuclear extracts of...
Konrad, E. Bruce, Lehman, I. R.
A mutant strain of E. coli, initially identified by an abnormally high frequency of recombination, has been found to be defective in the 5′ → 3′ exonuclease associated with DNA polymerase I,...
Enzymatic Characterization of a Mutant of Escherichia coli with an Altered DNA Ligase
A temperature-sensitive, radiation-sensitive mutant of Escherichia coli has been assayed for DNA ligase activity in vitro. The strain contains a markedly reduced amount of DNA-joining activity, which...
Gumport, Richard I., Lehman, I. R.
Proteolytic degradation of the Escherichia coli DNA ligase-adenylate intermediate releases adenosine 5′-monophosphate linked to the ε-amino group of lysine by a phosphoamide bond. Measurements of...
Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA.
Tye, B K, Nyman, P O, Lehman, I R, Hochhauser, S, Weiss, B
Strains of Escherichia coli with a mutation in the sof (dnaS) locus show a higher than normal frequency of recombination (are hyper rec) and incorporate label into short (4-5S) DNA fragments...
Isolation of an intact DNA polymerase-primase from embryos of Drosophila melanogaster.
Kaguni, L S, Rossignol, J M, Conaway, R C, Lehman, I R
A procedure has been devised for the purification of intact DNA polymerase alpha from early embryos of Drosophila melanogaster. The purified enzyme consists of at least three polypeptides with Mrs of...
The renaturation of complementary DNA strands by the recA protein of Escherichia coli has been found to exhibit the following features. (i) Optimal renaturation occurs at recA protein levels below...
Tye, Bik-Kwoon, Chien, Janice, Lehman, I. R., Duncan, Bruce K., Warner, Huber R.
Uracil is incorporated into newly synthesized DNA by mutants of Escherichia coli with reduced levels of dUTPase (dUTP nucleotidohydrolase; EC 3.6.1.23). Excision-repair of the incorporated uracil...
Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates.
A new group of mutants has been isolated which, during short pulses, incorporate (3-H)thymidine into DNA fragments that are substantially smaller than Okazaki fragments. These small fragments can be...
Cameron, J R, Panasenko, S M, Lehman, I R, Davis, R W
DNA from lambdagt-lambdaB bacteriophage was cleaved with EcoRI endonuclease and fragments from EcoRI-digested E. coli DNA were inserted. This DNA was used to infect E. coli, and phages containing the...
Makhov, A M, Boehmer, P E, Lehman, I R, Griffith, J D
The UL9 protein of herpes simplex virus type 1 (HSV-1) binds specifically to the HSV-1 oriS and oriL origins of replication, and is a DNA helicase and DNA-dependent NTPase. In this study electron...
Bessman, Maurice J., Lehman, I. R., Adler, Julius, Zimmerman, Steven B., Simms, E. S., Kornberg, Arthur
Lehman, I. R., Zimmerman, Steven B., Adler, Julius, Bessman, Maurice J., Simms, E. S., Kornberg, Arthur
Lehman, I. R., Herriott, Roger M.
Assimilation of oxygen, inorganic phosphate, and ammonia nitrogen by normal T2 phage and T2 ghost-infected E. coli B was studied. The rate of oxygen and phosphorus uptake by ghost-infected bacteria...