I. Saito

Electron emission from conduction band of diamond with negative electron affinity (2009)

Yamaguchi, H., Masuzawa, T., Nozue, S., Kudo, Y., Saito, I., Koe, J., ...

Experimental evidence explaining the extremely low-threshold electron emission from diamond reported in 1996 has been obtained for the first time. Direct observation using combined ultraviolet...

Experimental Derivation of Relative Biological Effectiveness of A-Bomb Neutrons in Hiroshima and Nagasaki and Implications for Risk Assessment (2008)

M. S. Sasaki, T. Nomura, Y. Ejima, H. Utsumi, S. Endo, I. Saito, ...

Sasaki, M. S., Nomura, T., Ejima, Y., Utsumi, H., Endo, S., Saito, I., Itoh, T. and Hoshi, M. Experimental Derivation of Relative Biological Effectiveness of A-Bomb Neutrons in Hiroshima and Nagasaki...

Field emission from modified P-doped diamond surfaces with different barrier heights (2008)

Kudo, Y., Yamada, T., Yamaguchi, H., Masuzawa, T., Saito, I., ...

In this study, the field emission properties of P-doped diamond with various surface treatments were measured in order to understand the effects of surface treatments on the barrier height. The...

DNA hole transport on an electrode: Application to effective photoelectrochemical SNP typing (2006)

Okamoto, A, Kamei, T, Saito, I

Simple SNP typing assay using a base-discriminating fluorescent probe (2006)

Okamoto, A, Tainaka, K, Ochi, Y, Kanatani, K, Saito, I

Synthesis and properties of purine-type base-discriminating fluorescent (BDF) nucleosides: distinction of thymine by fluorescence-labeled deoxyadenosine derivatives (2005)

Saito, Y, Hanawa, K, Motegi, K, Omoto, K, Okamoto, A, Saito, I

Discrimination of single-nucleotide alterations by G-specific fluorescence quenching (2005)

Dohno, C, Saito, I

Stable, specific, and reversible base pairing via Schiff base (2005)

Dohno, C, Okamoto, A, Saito, I

Obituary of late Prof. Matsuura (2005)

Saito, I

Template-directed DNA photoligation via alpha-5-cyanovinyldeoxyuridine (2005)

Ogino, M, Yoshimura, Y, Nakazawa, A, Saito, I, Fujimoto, K

Template-directed photoreversible ligation of DNA via 7-carboxyvinyl-7-deaza-2 '-deoxyadenosine (2005)

Saito, I, Miyauchi, Y, Saito, Y, Fujimoto, K

Fluorometric sensing of the salt-induced B-Z DNA transition by combination of two pyrene-labeled nucleobases (2005)

Okamoto, A, Ochi, Y, Saito, I

Design of base-discriminating fluorescent nucleosides (2005)

Okamoto, A, Saito, Y, Saito, I

Photoinduced DNA end capping via N-3-methyl-5-cyanovinyl-2 '-deoxyuridine (2005)

Fujimoto, K, Yoshimura, Y, Ikemoto, T, Nakazawa, A, Hayashi, M, Saito, I

Modulation of base selectivity for a base-discriminating fluorescent nucleobase by addition of mercury ion (2005)

Okamoto, A, Ochi, Y, Saito, I

Photochemistry of naphthalene diimides: EPR study of free radical formation via photoredox process (2005)

Reszka, KJ, Takayama, M, Sik, RH, Chignell, CF, Saito, I

A dielectric-sensitive fluorescent DNA probe for monitoring polarities on the interior of a DNA-binding protein (2005)

Okamoto, A, Tainaka, K, Saito, I

Public-key system using DNA as a one-way function for key distribution (2005)

Tanaka, K, Okamoto, A, Saito, I

Synthesis and ESR studies of nitronyl nitroxide-tethered oligodeoxynucleotides (2005)

Okamoto, A, Inasaki, T, Saito, I

Monitoring DNA structures by dual fluorescence of pyrene derivatives (2005)

Okamoto, A, Tainaka, K, Nishiza, K, Saito, I

The SPR sensor detecting cytosine-cytosine mismatches (2004)

Kobori, A, Horie, S, Suda, H, Saito, I, Nakatani, K

Synthesis and properties of novel base-discriminating fluorescent (BDF) nucleosides: a highly polarity-sensitive fluorophore for SNP typing (2004)

Saito, Y, Miyauchi, Y, Okamoto, A, Saito, I

Cytosine detection by a fluorescein-labeled probe containing base-discriminating fluorescent nucleobase (2004)

Okamoto, A, Tanaka, K, Fukuta, T, Saito, I

Base-discriminating fluorescent (BDF) nucleoside: distinction of thymine by fluorescence quenching (2004)

Saito, Y, Miyauchi, Y, Okamoto, A, Saito, I

2-Ureidoquinoline: a useful molecular element for stabilizing single cytosine and thymine bulges (2004)

Kobori, A, Murase, T, Suda, H, Saito, I, Nakatani, K

Charge transport in duplex DNA containing modified nucleotide bases (2004)

Nakatani, K, Saito, I

Synthesis of an artificial hole-transporting nucleoside triphosphate, d(MD) ATP, and its enzymatic incorporation into DNA (2004)

Okamoto, A, Tanaka, K, Nishiza, K, Saito, I

The binding of guanine-guanine mismatched DNA to naphthyridine dimer immobilized sensor surfaces: kinetic aspects (2004)

Nakatani, K, Kobori, A, Kumasawa, H, Goto, Y, Saito, I

DNA logic gates (2004)

Okamoto, A, Tanaka, K, Saito, I

Highly sensitive detection of GG mismatched DNA by surfaces immobilized naphthyridine dimer through poly(ethylene oxide) linkers (2004)

Nakatani, K, Kobori, A, Kumasawa, H, Saito, I

Nitroxide-labeled guanine as an ESR spin probe for structural study of DNA (2004)

Okamoto, A, Inasaki, T, Saito, I

A nucleobase that releases reporter tags upon DNA oxidation (2004)

Okamoto, A, Tanaka, K, Saito, I

Pyrene-labeled oligodeoxynucleotide probe for detecting base insertion by excimer fluorescence emission (2004)

Okamoto, A, Ichiba, T, Saito, I

Detection of guanine-adenine mismatches by surface plasmon resonance sensor carrying naphthyridine-azaquinolone hybrid on the surface (2004)

Hagihara, S, Kumasawa, H, Goto, Y, Hayashi, G, Kobori, A, Saito, I, ...

Pyrene-labeled base-discriminating fluorescent DNA probes for homogeneous SNP typing (2004)

Okamoto, A, Kanatani, K, Saito, I

Photostimulated hole transport through a DNA duplex immobilized on a gold electrode (2004)

Okamoto, A, Kamei, T, Tanaka, K, Saito, I

A novel fluorescent guanine derivative distinguishable of three structures, single strand, duplex, and quadruplex (2004)

Okamoto, A, Kanatani, K, Ochi, Y, Saito, Y, Saito, I

Detection of A/G single nucleotide alteration in RNA using base-discriminating fluorescent oligodeoxynucleotides (2003)

Okamoto, A, Tainaka, K, Saito, I

Clear distinction of purine bases on the complementary strand by a fluorescence change of a novel fluorescent nucleoside (2003)

Okamoto, A, Tainaka, K, Saito, I

Induction of a remarkable conformational change in a human telomeric sequence by the binding of naphthyridine dimer: Inhibition of the elongation of a telomeric repeat by telomerase (2003)

Nakatani, K, Hagihara, S, Sando, S, Sakamoto, S, Yamaguchi, K, Maesawa, C, ...

Design of base-discriminating fluorescent nucleoside and its application to T/C SNP typing (2003)

Okamoto, A, Tanaka, K, Fukuta, T, Saito, I

Assessment of the sequence dependency for the binding of 2-aminonaphthyridine to the guanine bulge (2003)

Nakatani, K, Horie, S, Murase, T, Hagihara, S, Saito, I

Rational design of a DNA wire possessing an extremely high hole transport ability (2003)

Okamoto, A, Tanaka, K, Saito, I

Phototriggered drug release from functionalized oligonucleotides by a molecular beacon strategy (2003)

Okamoto, A, Tanabe, K, Inasaki, T, Saito, I

N-15 NMR study on site-selective binding of metal ions to guanine runs in DNA: A good correlation with HOMO distribution (2003)

Okamoto, A, Kanatani, K, Taiji, T, Saito, I

Hole trapping at N-6-cyclopropyideoxyadenosine suggests a direct contribution of adenine bases to hole transport through DNA (2003)

Dohno, C, Ogawa, A, Nakatani, K, Saito, I

Ab initio molecular orbital study on the G-selectivity of GGG triplet in copper(I)-mediated one-electron oxidation (2003)

Yoshioka, Y, Kawai, H, Sato, T, Yamaguchi, K, Saito, I

P-loop catalytically assisting the enzymatic cleavage of single-stranded DNA (2003)

Okamoto, A, Tanabe, K, Saito, I

Synthesis and properties of a novel fluorescent nucleobase, naphthopyridopyrimidine (2003)

Okamoto, A, Tainaka, K, Saito, I

Formation and destruction of the guanine quartet in solution observed by cold-spray ionization mass spectrometry (2003)

Sakamoto, S, Nakatani, K, Saito, I, Yamaguchi, K

Affinity labeling of a single guanine bulge (2003)

Nakatani, K, Horie, S, Saito, I

Highly efficient sequence-specific DNA interstrand cross-linking by pyrrole/imidazole CPI conjugates (2003)

Bando, T, Narita, A, Saito, I, Sugiyama, H

Detection of C-C mismatched base pair by SPR assay using sensors immobilized with a cytosine-specific ligand. (2003)

Kobori, A, Nakatani, K, Saito, I

Bis-pyrene labeled DNA aptamer as an intelligent fluorescent biosensor (2003)

Yamana, K, Ohtani, Y, Nakano, H, Saito, I

Development of novel modified nucleobases as a fluorescence probe for detection of single nucleotide polymorphisms. (2002)

Tainaka, K, Okamoto, A, Saito, I

N-15-NMR analysis of interaction between DNA containing N-15-labeled guanine and paramagnetic metals. (2002)

Kanatani, K, Okamoto, A, Saito, I

Oligonucleotides containing 7-vinyl-7-deazaguanine as a facile strategy for expanding the functional diversity of DNA (2002)

Okamoto, A, Taiji, T, Tainaka, K, Saito, I

Unique hole-trapping property of the degenerate base, 2-amino-7-deazaadenine (2002)

Okamoto, A, Tanaka, K, Saito, I

Site-specific discrimination of cytosine and 5-methylcytosine in duplex DNA by peptide nucleic acids (2002)

Okamoto, A, Tanabe, K, Saito, I

Suppression of DNA-mediated charge transport by BamHI binding (2002)

Nakatani, K, Dohno, C, Ogawa, A, Saito, I

Molecular design of a pyrrole-imidazole hairpin polyamides for effective DNA alkylation (2002)

Bando, T, Narita, A, Saito, I, Sugiyama, H

Recognition of DNA mismatches by small molecules. (2002)

Hagihara, S, Nakatani, K, Saito, I

Photochemistry of benzophenone immobilized in a major groove of DNA: Formation of thermally reversible interstrand cross-link (2002)

Nakatani, K, Yoshida, T, Saito, I

Chemically induced hairpin formation in DNA monolayers (2002)

Smith, EA, Kyo, M, Kumasawa, H, Nakatani, K, Saito, I, Corn, RM

Modulation of remote DNA oxidation by hybridization with peptide nucleic acids (PNA) (2002)

Okamoto, A, Tanabe, K, Dohno, C, Saito, I

2-amino-7-deazaadenine forms stable base pairs with cytosine and thymine (2002)

Okamoto, A, Tanaka, K, Saito, I

Deoxyribonolactone formation in photoirradiation of 5-bromouracil-containing oligonucleotides by direct C1 ' hydrogen abstraction (2002)

Fujimoto, K, Ikeda, Y, Ishihara, S, Saito, I

Synthesis and characterization of naphthalimide-containing peptide nucleic acid (2002)

Ikeda, H, Nakamura, Y, Saito, I

N-2-Phenyldeoxyguanosine: Modulation of the chemical properties of deoxyguanosine toward one-electron oxidation in DNA (2002)

Nakatani, K, Dohno, C, Saito, I

Site-specific hydroxylation at polyguanosine in double-stranded DNA by nickel(II) in the presence of SH compounds: Comparison with singlet oxygen-induced DNA damage (2002)

Oikawa, S, Hiraku, Y, Fujiwara, T, Saito, I, Kawanishi, S

A facile incorporation of the aldehyde function into DNA: 3-formylindole nucleoside as an aldehyde-containing universal nucleoside (2002)

Okamoto, A, Tainaka, K, Saito, I

Guanine of the third strand of C center dot G*G triplex serves as an effective hole trap (2002)

Dohno, C, Nakatani, K, Saito, I

Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance (2001)

Nakatani, K, Sando, S, Saito, I

Synthesis and characterization of flavin-tethered peptide nucleic acid (2001)

Ikeda, H, Yoshida, K, Ozeki, M, Saito, I

Synthesis and properties of peptide nucleic acids containing a psoralen unit (2001)

Okamoto, A, Tanabe, K, Saito, I

Specific binding of 2-amino-1,8-naphthyridine into a single guanine bulge as evidenced by photooxidation of GG doublet (2001)

Nakatani, K, Sando, S, Yoshida, K, Saito, I

Selective intercalation of charge neutral intercalators into GG and CG steps: Implication of HOMO-LUMO interaction for sequence-selective drug intercalation into DNA (2001)

Nakatani, K, Matsuno, T, Adachi, K, Hagihara, S, Saito, I

Sequence-specific DNA interstrand cross-linking by imidazole-pyrrole CPI conjugate (2001)

Bando, T, Iida, H, Saito, I, Sugiyama, H

Sequence selectivity for the guanine alkylation by DNA alkylating intercalators (2001)

Nakatani, K, Saito, I

Improved selectivity for the binding of naphthyridine dimer to guanine-guanine mismatch (2001)

Nakatani, K, Sando, S, Saito, I

Amplification of bleomycin-induced DNA cleavage at cytosine residues 3 ' to GGG sequences by pyrrole triamide (2001)

Hiraku, Y, Oikawa, S, Kuroki, K, Sugiyama, H, Saito, I, Kawanishi, S

Recognition of guanine-guanine mismatches by the dimeric form of 2-amino-1,8-naphthyridine (2001)

Nakatani, K, Sando, S, Kumasawa, H, Kikuchi, J, Saito, I

Template-directed reversible photocircularization of DNA via 5-vinyldeoxycytidine (2000)

Fujimoto, K, Matsuda, S, Ogawa, N, Hayashi, M, Saito, I

DNA-targeting drug design (2000)

Saito, I

Metallo-bleomycin cleaves parallel-stranded DNA similarly to B-DNA (2000)

Ikeda, S, Saito, I, Sugiyama, H

Reversible DNA photocircularization on triple helix: effect of vinyl substituent on base stacking (2000)

Fujimoto, K, Matsuda, S, Hayashi, M, Saito, I

Mapping of the highest occupied molecular orbital of a DNA-RNA hybrid by cobalt-benzoyl peroxide oxidation (2000)

Nakamura, T, Saito, I

Template-directed photoreversible ligation of deoxyoligonucleotides via 5-vinyldeoxyuridine (2000)

Fujimoto, K, Matsuda, S, Takahashi, N, Saito, I

Site selective formation of thymine glycol-containing oligodeoxynucleotides by oxidation with osmium tetroxide and bipyridine-tethered oligonucleotide (2000)

Nakatani, K, Hagihara, S, Sando, S, Miyazaki, H, Tanabe, K, Saito, I

Recognition of a single guanine bulge by 2-acylamino-1,8-naphthyridine (2000)

Nakatani, K, Sando, S, Saito, I

Highly cooperative DNA dialkylation by the homodimer of imidazole-pyrrole diamide-CPI conjugate with vinyl linker (2000)

Tao, ZF, Saito, I, Sugiyama, H

Site-selective DNA alkylation of GG steps by naphthaldiimide derivatives possessing enantiomeric epoxide (2000)

Okamoto, A, Nakamura, T, Yoshida, K, Nakatani, K, Saito, I

Site selective generation of guanine radical cation in duplex DNA: modulation of the direction of hole transport (2000)

Nakatani, K, Dohno, C, Saito, I

Direct strand cleavage via furanyladenine formation in anaerobic photoirradiation of 5-bromouracil-containing oligonucleotides (2000)

Fujimoto, K, Ikeda, Y, Saito, I

Amplification of pepleomycin-mediated DNA cleavage and apoptosis by unfused aromatic cations (2000)

Kawanishi, S, Oikawa, S, Kawanishi, M, Sugiyama, H, Saito, I, Strekowski, L, ...

Modulation of DNA-mediated hole-transport efficiency by changing superexchange electronic interaction (2000)

Nakatani, K, Dohno, C, Saito, I

Template directed photochemical synthesis of branched oligodeoxynucleotides via 5-carboxyvinyldeoxyuridine (2000)

Fujimoto, K, Ogawa, N, Hayashi, M, Matsuda, S, Saito, I

Synthesis and duplex stability of oligonucleotides containing 7-vinyl-7-deazaguanine as a strong electron-donating nucleobase (2000)

Okamoto, A, Taiji, T, Tanaka, K, Saito, I

Mapping of highest occupied molecular orbitals of duplex DNA by cobalt-mediated guanine oxidation (2000)

Saito, I, Nakamura, T, Nakatani, K

Site-specific oxidation at GG and GGG sequences in double-stranded DNA by benzoyl peroxide as a tumor promoter (1999)

Kawanishi, S, Oikawa, S, Murata, M, Tsukitome, H, Saito, I

Serotyping of quinolone-resistant Neisseria gonorrhoeae isolates with alterations in GyrA and ParC (1998)

Deguchi, T, Yasuda, M, Maeda, SI, Saito, I, Kawada, Y

In-vitro antimicrobial activity of HSR-903, a new fluoroquinolone, against clinical isolates of Neisseria gonorrhoeae with quinolone resistance-associated alterations in GyrA and ParC (1997)

Deguchi, T, Yasuda, M, Ishihara, S, Takahashi, Y, Okezaki, E, Nagata, O, ...

Antimicrobial activity of a new fluoroquinolone, DU-6859a, against quinolone-resistant clinical isolates of Neisseria gonorrhoeae with genetic alterations in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV (1997)

Deguchi, T, Yasuda, M, Nakano, M, Kanematsu, E, Ozeki, S, Ishihara, S, ...

Synthesis, structure and thermodynamic properties of 8-methylguanine- containing oligonucleotides: Z-DNA under physiological salt conditions (1996)

Sugiyama, H, Kawai, K, Matsunaga, A, Fujimoto, K, Saito, I, Robinson, H, ...

Expression of inflammatory cytokines and adhesion molecules in haemodialysis-associated amyloidosis (1995)

Inoue, H., Saito, I., Nakazawa, R., Mukaida, N., Matsushima, K., Azuma, N., ...

Background. The occurrence of various arthropathies including carpal-tunnel syndrome (CTS) in dialysis-associated amyloidosis, a condition caused by the deposition of β2 microglobulin (β2MG), has...

Eluate from {beta}2MG adsorbent beads enhances the expression of IL-1{beta} from human monocyte (1995)

Inoue, H., Saito, I., Nakazawa, R., Azuma, N., Suzuki, M., Miyasaka, N.

Nucleotide sequence of the hepatitis C virus genome from a patient negative for anti-HCV by the first generation antibody assay (1993)

Sarashina, T., Sakurai, T., Watanabe, Y., Kashima, K., Suzuki, T., Chiba, J., ...

Nucleotide sequence of core and envelope genes of the hepatitis C virus genome derived directly from human healthy carriers (1990)

Takeuchi, K, Kubo, Y, Boonmar, S, Watanabe, Y, Katayama, T, Choo, QL, ...

Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome.

Miyake, S, Makimura, M, Kanegae, Y, Harada, S, Sato, Y, Takamori, K, ...

An efficient method of constructing recombinant adenoviruses (Ads) has been established. The expression unit to be introduced into recombinant Ad was first inserted into the unique Swa I site of the...

A histologically distinctive interstitial pneumonia induced by overexpression of the interleukin 6, transforming growth factor beta 1, or platelet-derived growth factor B gene.

Yoshida, M, Sakuma, J, Hayashi, S, Abe, K, Saito, I, Harada, S, ...

Interstitial pneumonia is characterized by alveolitis with resulting fibrosis of the interstitium. To determine the relevance of humoral factors in the pathogenesis of interstitial pneumonia, we...

Adenovirus-mediated transfer of the HST-1 (FGF4) gene induces increased levels of platelet count in vivo.

Sakamoto, H, Ochiya, T, Sato, Y, Tsukamoto, M, Konishi, H, Saito, I, ...

The HST-1 (fibroblast growth factor 4, FGF4) protein is a potent mitogen for a variety of cell types of mesodermal and neuroectodermal origin, including fibroblasts, endothelial cells, and...

Serodiagnosis of hepatitis C virus (HCV) infection with an HCV core protein molecularly expressed by a recombinant baculovirus.

Chiba, J, Ohba, H, Matsuura, Y, Watanabe, Y, Katayama, T, Kikuchi, S, ...

An enzyme-linked immunosorbent assay (ELISA) was developed for serological diagnosis of hepatitis C virus (HCV) infection, using HCV core protein (p22) synthesized by a recombinant baculovirus. Among...

Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: application to diagnosis and blood screening for posttransfusion hepatitis.

Miyamura, T, Saito, I, Katayama, T, Kikuchi, S, Tateda, A, Houghton, M, ...

A cDNA clone has been derived from the plasma of a chimpanzee with chronic non-A, non-B viral hepatitis (NANBH). We have assayed for antibodies reacting with the encoded antigen in sera from...

Hepatitis C virus infection is associated with the development of hepatocellular carcinoma.

Saito, I, Miyamura, T, Ohbayashi, A, Harada, H, Katayama, T, Kikuchi, S, ...

A possible causative role for the recently discovered hepatitis C virus (HCV) in the development of hepatocellular carcinoma (HCC) was investigated by assay of sera from HCC patients in Japan for...

Synthesis, structure and thermodynamic properties of 8-methylguanine-containing oligonucleotides: Z-DNA under physiological salt conditions.

Sugiyama, H, Kawai, K, Matsunaga, A, Fujimoto, K, Saito, I, Robinson, H, ...

Various oligonucleotides containing 8-methylguanine (m8G) have been synthesized and their structures and thermodynamic properties investigated. Introduction Of M8G into DNA sequences markedly...

Replication of DNA templates containing 5-formyluracil, a major oxidative lesion of thymine in DNA.

Zhang, Q M, Sugiyama, H, Miyabe, I, Matsuda, S, Saito, I, Yonei, S

5-Formyluracil (5-foU) is a major lesion of thymine produced in DNA by ionizing radiation and various chemical oxidants. To assess its biochemical effects on DNA replication, 22mer oligonucleotide...

DNA gyrase mutations in quinolone-resistant clinical isolates of Neisseria gonorrhoeae.

Deguchi, T, Yasuda, M, Asano, M, Tada, K, Iwata, H, Komeda, H, ...

Eight quinolone-resistant clinical isolates of Neisseria gonorrhoeae were shown to carry mutations in their GyrA proteins. Six isolates had a single amino acid change of serine to phenylalanine at...

Quinolone-resistant Neisseria gonorrhoeae: correlation of alterations in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV with antimicrobial susceptibility profiles.

Deguchi, T, Yasuda, M, Nakano, M, Ozeki, S, Ezaki, T, Saito, I, ...

Fifty-five clinical strains of Neisseria gonorrhoeae were examined for mutations in the gyrA and parC genes and for antimicrobial susceptibility profiles. The MICs of quinolones for 31 strains with...

Uncommon occurrence of mutations in the gyrB gene associated with quinolone resistance in clinical isolates of Neisseria gonorrhoeae.

Deguchi, T, Yasuda, M, Nakano, M, Ozeki, S, Kanematsu, E, Kawada, Y, ...

Incomplete transformation of rat cells by a deletion mutant of adenovirus type 5.

Shiroki, K, Maruyama, K, Saito, I, Fukui, Y, Shimojo, H

Rat 3Y1 cells were infected with adenovirus type 5 (Ad5) wild type, dl312 (deletion of 902 base pairs between 1.5-4.5 map units), and dl313 (deletion of 2,350 base pairs between 3.5-10.5 map units)....

Soluble CD14 mediates lipopolysaccharide-induced intercellular adhesion molecule 1 expression in cultured human gingival fibroblasts.

Hayashi, J, Masaka, T, Saito, I, Ishikawa, I

Intercellular adhesion molecule 1 (ICAM-1) is involved in the accumulation and activation of leukocytes in inflammatory diseases such as periodontitis. As reported previously, ICAM-1 is up-regulated...

Effective prevention of thrombocytopenia in mice using adenovirus-mediated transfer of HST-1 (FGF-4) gene.

Konishi, H, Ochiya, T, Sakamoto, H, Tsukamoto, M, Saito, I, Muto, T, ...

HST-1 (FGF-4) gene product is a member of the fibroblast growth factor family with a signal peptide and plays a crucial role in limb development. We showed previously that an intraperitoneal...

Nitric oxide production and inducible nitric oxide synthase expression in inflammatory arthritides.

Sakurai, H, Kohsaka, H, Liu, M F, Higashiyama, H, Hirata, Y, Kanno, K, ...

In this study, we have identified the source of nitric oxide (NO) produced in the human inflammatory joints by analyzing expression of inducible NO synthase. In ex vivo organ cultures, both...

Renal dehydropeptidase-I stability of LJC 10,627, a new carbapenem antibiotic.

Hikida, M, Kawashima, K, Nishiki, K, Furukawa, Y, Nishizawa, K, Saito, I, ...

LJC 10,627 is a new parenteral carbapenem antibiotic. LJC 10,627 stability against human renal dehydropeptidase-I was compared with that of imipenem. Hydrolysis of this compound was not detectable by...

Gene targeting with a replication-defective adenovirus vector.

Fujita, A, Sakagami, K, Kanegae, Y, Saito, I, Kobayashi, I

Wide application of the gene-targeting technique has been hampered by its low level of efficiency. A replication-defective adenovirus vector was used for efficient delivery of donor DNA in order to...

Comparison among performances of a ligase chain reaction-based assay and two enzyme immunoassays in detecting Chlamydia trachomatis in urine specimens from men with nongonococcal urethritis.

Deguchi, T, Yasuda, M, Uno, M, Tada, K, Iwata, H, Komeda, H, ...

We evaluated the performances of a ligase chain reaction (LCR)-based assay and two enzyme immunoassays (Chlamydiazyme and IDEIA) in the detection of Chlamydia trachomatis in urine specimens. We...

Rapid detection of point mutations of the Neisseria gonorrhoeae gyrA gene associated with decreased susceptibilities to quinolones.

Deguchi, T, Yasuda, M, Nakano, M, Ozeki, S, Ezaki, T, Maeda, S, ...

Mutations in the gyrA gene resulting in amino acid changes at Ser-91 and Asp-95 are significantly associated with decreased susceptibilities to quinolones in Neisseria gonorrhoeae. To detect these...

Rapid screening of point mutations of the Neisseria gonorrhoeae parC gene associated with resistance to quinolones.

Deguchi, T, Yasuda, M, Nakano, M, Kanematsu, E, Ozeki, S, Nishino, Y, ...

To detect quinolone resistance-associated mutations within the Asp-86, Ser-87, Ser-88, and Glu-91 codons of the Neisseria gonorrhoeae parC gene, we developed a rapid and simple assay based on...

Expression of processed envelope protein of hepatitis C virus in mammalian and insect cells.

Matsuura, Y, Harada, S, Suzuki, R, Watanabe, Y, Inoue, Y, Saito, I, ...

The putative envelope protein of hepatitis C virus (HCV) was expressed in insect cells by using a baculovirus expression vector and in monkey COS cells under the control of exogenous promoters. The...

Expression of processed core protein of hepatitis C virus in mammalian cells.

Harada, S, Watanabe, Y, Takeuchi, K, Suzuki, T, Katayama, T, Takebe, Y, ...

A structural protein of hepatitis C virus (HCV) was expressed in monkey COS cells under the control of an exogenous promoter, and a protein of 22 kDa was identified by immunoblot analysis. This...

Novel RNA family structure of hepatitis B virus expressed in human cells, using a helper-free adenovirus vector.

Saito, I, Oya, Y, Shimojo, H

Ad5-HBL is a type 5 adenovirus bearing the large BglII fragment (2.8 kilobases; 87% of the total genome) of hepatitis B virus (HBV), subtype adr. Eight HBV RNAs expressed in HeLa cells infected with...

Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end of its genome.

Saito, I, Oya, Y, Yamamoto, K, Yuasa, T, Shimojo, H

A novel helper-free adenovirus type 5 (Ad5) vector system, which utilizes a cloning site 0.2 kilobase (kb) from the right end of the genome, has been developed. To construct a nondefective Ad5...

Isolation of a nondefective recombinant between adenovirus type 5 and early region 1A of adenovirus type 12.

Shiroki, K, Saito, I, Maruyama, K, Shimojo, H

A nondefective recombinant between adenovirus type 5 (Ad5) and type 12 (Ad12), rc-1 (Ad5 dl312, carrying the Ad12 E1A gene), was isolated from hamster cell foci transformed by a defective...

Isolation of transformation-defective, replication-nondefective early region 1B mutants of adenovirus 12.

Fukui, Y, Saito, I, Shiroki, K, Shimojo, H

We isolated three adenovirus 12 early region 1B mutants (in205B, in205C, and dl205) by ligation of the cleaved DNA-protein complex and transfection of human embryo kidney cells with the ligation...

Characterization of a host range mutant of human adenovirus 12 defective in early region 1B.

Fukui, Y, Shiroki, K, Saito, I, Shimojo, H

We isolated an adenovirus 12 early region 1B mutant (in206B) by ligation of the cleaved DNA-protein complex and transfection of human embryo kidney cells with the ligation products. By deduction from...

Expression of the E4 gene is required for establishment of soft-agar colony-forming rat cell lines transformed by the adenovirus 12 E1 gene.

Shiroki, K, Hashimoto, S, Saito, I, Fukui, Y, Fukui, Y, Kato, H, ...

Rat 3Y1 cells were transfected with recombinant gARC ( pSV2gpt carrying the adenovirus 12 early region 1 [E1] gene), and focus formation was observed in monolayer cultures after culture of cells in...

Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector.

Oda, K, Kato, H, Saito, I, Sugano, S, Maruyama, K, Masuda, M, ...

Simian virus 40 (SV40) recombinants carrying the adenovirus type 12 E1A gene were constructed. The SV40 expression vector was constructed by removing most of the VP1 gene and an internal part of the...

Hyperproduction of adenovirus type 12 E1B gene product in monkey cells, using a simian virus 40 vector.

Oda, K, Fukui, Y, Saito, I, Masuda, M, Shiroki, K, Shimojo, H

Simian virus 40 recombinant DNAs carrying the adenovirus type 12 E1B gene were constructed, propagated, and packaged in monkey cells. Monkey cells infected with the resulting virus stocks...

Expression of adenovirus type 12 early region 1 in KB cells transformed by recombinants containing the gene.

Shiroki, K, Saito, I, Maruyama, K, Fukui, Y, Imatani, Y, Oda, K I, ...

The adenovirus type 12 (Ad12) early region 1 (E1) gene was introduced into KB cells by using a dominant selection vector, pSV2-gpt, and over 80 Gpt+ KB cell clones were established. Three types of...

Dependence of tumor-forming capacities of cells transformed by recombinants between adenovirus types 5 and 12 on expression of early region 1.

Shiroki, K, Maruyama, K, Saito, I, Fukui, Y, Yazaki, K, Shimojo, H

Recombinants between an adenovirus type 5 (Ad5) deletion mutant and the Ad12 DNA fragment containing early region 1 (E1) were isolated from cells cotransfected with the EcoRI-C fragment of Ad12 DNA...

Discrimination of hepatitis C virus in liver tissues from different patients with hepatocellular carcinomas by direct nucleotide sequencing of amplified cDNA of the viral genome.

Chou, W H, Yoneyama, T, Takeuchi, K, Harada, H, Saito, I, Miyamura, T

We investigated the presence of the hepatitis C virus (HCV) genome in liver tissues of eight different patients with hepatocellular carcinoma by using the reverse transcriptase polymerase chain...

Detection and mapping of spliced RNA from a human hepatoma cell line transfected with the hepatitis B virus genome.

Suzuki, T, Masui, N, Kajino, K, Saito, I, Miyamura, T

HepG2 cells, known to support the replication and virion formation of hepatitis B virus (HBV), were transfected with a cosmid constructed to contain 12 tandem head-to-tail repeats of the HBV genome...

Effects of cytokines and periodontopathic bacteria on the leukocyte function-associated antigen 1/intercellular adhesion molecule 1 pathway in gingival fibroblasts in adult periodontitis.

Hayashi, J, Saito, I, Ishikawa, I, Miyasaka, N

We investigated the effects of inflammatory cytokines and periodontopathic bacteria on expression of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1, and E-selectin...

Enhancement of retrovirus-mediated gene transduction efficiency by transient overexpression of the amphotropic receptor, GLVR-2.

Yamaguchi, S, Wakimoto, H, Yoshida, Y, Kanegae, Y, Saito, I, Aoyagi, M, ...

Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.

Kanegae, Y, Lee, G, Sato, Y, Tanaka, M, Nakai, M, Sakaki, T, ...

A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian cells, another recombinant Ad bearing an...

Nucleotide sequence of the hepatitis C virus genome from a patient negative for anti-HCV by the first generation antibody assay.

Sarashina, T, Sakurai, T, Watanabe, Y, Kashima, K, Suzuki, T, Chiba, J, ...

A new procedure for determining thymine residues in DNA sequencing. Photoinduced cleavage of DNA fragments in the presence of spermine.

Saito, I, Sugiyama, H, Matsuura, T, Ueda, K, Komano, T

A new procedure for T specific cleavage of DNA fragments utilizing photoreaction with spermine has been described. Irradiation of 3'-[32P]-end-labeled DNA fragments for 10-20 min with a germicidal...

Nucleotide sequence of core and envelope genes of the hepatitis C virus genome derived directly from human healthy carriers.

Takeuchi, K, Kubo, Y, Boonmar, S, Watanabe, Y, Katayama, T, Choo, Q L, ...

A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non-A, non-B hepatitis in Japan.

Kubo, Y, Takeuchi, K, Boonmar, S, Katayama, T, Choo, Q L, Kuo, G, ...

Recently, a cDNA from the hepatitis C virus (HCV) RNA genome has been isolated in the USA from a chronically infected chimpanzee. In order to isolate HCV cDNA derived from human material, RNA was...

Evolution and stability of chromosomal DNA coamplified with the CAD gene.

Saito, I, Groves, R, Giulotto, E, Rolfe, M, Stark, G R

We have compared clones of Syrian hamster cells selected for the first amplification of the CAD gene with clones selected for further amplification. The large domain amplified initially was not...

Preferential amplification of rearranged sequences near amplified adenylate deaminase genes.

Debatisse, M, Saito, I, Buttin, G, Stark, G R

In a previous study of three independent families of mutants selected for overproduction of adenylate deaminase (AMPD), we were not able to isolate a cDNA probe for the gene and so could not...

Charomids: cosmid vectors for efficient cloning and mapping of large or small restriction fragments.

Saito, I, Stark, G R

Charomids are cosmid vectors up to 52 kilobases (kb) long, bearing 1-23 copies of a 2-kb spacer fragment linked in head-to-tail tandem arrays. Like cosmids and lambda phage, charomids can be packaged...

Retrovirus in salivary glands from patients with Sjögren's syndrome.

Yamano, S, Renard, J N, Mizuno, F, Narita, Y, Uchida, Y, Higashiyama, H, ...

AIMS: To investigate the possibility of an immune response to retroviral antigens or of detecting retrovirus in Sjögren's syndrome. METHODS: Retroviruses were sought in labial salivary glands and...

Possible involvement of IL-12 expression by Epstein-Barr virus in Sjögren syndrome.

Horiuchi, M, Yamano, S, Inoue, H, Ishii, J, Nagata, Y, Adachi, H, ...

AIM: To determine the correlation between interleukin 12 (IL-12) expression and Epstein-Barr virus (EBV) in Sjögren syndrome. METHODS: Indirect immunohistochemical technique, enzyme linked...

Local expression of immunoregulatory IL-12p40 gene prolonged syngeneic islet graft survival in diabetic NOD mice.

Yasuda, H, Nagata, M, Arisawa, K, Yoshida, R, Fujihira, K, Okamoto, N, ...

Local production of immunosuppressive cytokines will be one of the most suitable therapeutic strategies against organ-specific autoimmune diabetes. To establish such a new therapy, we constructed...

Structure of DNA formed in the first step of CAD gene amplification.

Giulotto, E, Saito, I, Stark, G R

Thirty-three independent mutant cell lines were selected in single steps for resistance to low concentrations of N-(phosphonacetyl)-L-aspartate and the structure of their amplified DNA was probed,...

Structural studies of a stable parallel-stranded DNA duplex incorporating isoguanine:cytosine and isocytosine:guanine basepairs by nuclear magnetic resonance spectroscopy.

Yang, X L, Sugiyama, H, Ikeda, S, Saito, I, Wang, A H

Isoguanine (2-hydroxyladenine) is a product of oxidative damage to DNA and has been shown to cause mutation. It is also a potent inducer of parallel-stranded DNA duplex structure. The structure of...

Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome.

Miyake, S, Makimura, M, Kanegae, Y, Harada, S, Sato, Y, Takamori, K, ...

An efficient method of constructing recombinant adenoviruses (Ads) has been established. The expression unit to be introduced into recombinant Ad was first inserted into the unique Swa I site of the...

A histologically distinctive interstitial pneumonia induced by overexpression of the interleukin 6, transforming growth factor beta 1, or platelet-derived growth factor B gene.

Yoshida, M, Sakuma, J, Hayashi, S, Abe, K, Saito, I, Harada, S, ...

Interstitial pneumonia is characterized by alveolitis with resulting fibrosis of the interstitium. To determine the relevance of humoral factors in the pathogenesis of interstitial pneumonia, we...

Adenovirus-mediated transfer of the HST-1 (FGF4) gene induces increased levels of platelet count in vivo.

Sakamoto, H, Ochiya, T, Sato, Y, Tsukamoto, M, Konishi, H, Saito, I, ...

The HST-1 (fibroblast growth factor 4, FGF4) protein is a potent mitogen for a variety of cell types of mesodermal and neuroectodermal origin, including fibroblasts, endothelial cells, and...

Serodiagnosis of hepatitis C virus (HCV) infection with an HCV core protein molecularly expressed by a recombinant baculovirus.

Chiba, J, Ohba, H, Matsuura, Y, Watanabe, Y, Katayama, T, Kikuchi, S, ...

An enzyme-linked immunosorbent assay (ELISA) was developed for serological diagnosis of hepatitis C virus (HCV) infection, using HCV core protein (p22) synthesized by a recombinant baculovirus. Among...

Detection of antibody against antigen expressed by molecularly cloned hepatitis C virus cDNA: application to diagnosis and blood screening for posttransfusion hepatitis.

Miyamura, T, Saito, I, Katayama, T, Kikuchi, S, Tateda, A, Houghton, M, ...

A cDNA clone has been derived from the plasma of a chimpanzee with chronic non-A, non-B viral hepatitis (NANBH). We have assayed for antibodies reacting with the encoded antigen in sera from...

Hepatitis C virus infection is associated with the development of hepatocellular carcinoma.

Saito, I, Miyamura, T, Ohbayashi, A, Harada, H, Katayama, T, Kikuchi, S, ...

A possible causative role for the recently discovered hepatitis C virus (HCV) in the development of hepatocellular carcinoma (HCC) was investigated by assay of sera from HCC patients in Japan for...

Synthesis, structure and thermodynamic properties of 8-methylguanine-containing oligonucleotides: Z-DNA under physiological salt conditions.

Sugiyama, H, Kawai, K, Matsunaga, A, Fujimoto, K, Saito, I, Robinson, H, ...

Various oligonucleotides containing 8-methylguanine (m8G) have been synthesized and their structures and thermodynamic properties investigated. Introduction Of M8G into DNA sequences markedly...

Replication of DNA templates containing 5-formyluracil, a major oxidative lesion of thymine in DNA.

Zhang, Q M, Sugiyama, H, Miyabe, I, Matsuda, S, Saito, I, Yonei, S

5-Formyluracil (5-foU) is a major lesion of thymine produced in DNA by ionizing radiation and various chemical oxidants. To assess its biochemical effects on DNA replication, 22mer oligonucleotide...

DNA gyrase mutations in quinolone-resistant clinical isolates of Neisseria gonorrhoeae.

Deguchi, T, Yasuda, M, Asano, M, Tada, K, Iwata, H, Komeda, H, ...

Eight quinolone-resistant clinical isolates of Neisseria gonorrhoeae were shown to carry mutations in their GyrA proteins. Six isolates had a single amino acid change of serine to phenylalanine at...

Quinolone-resistant Neisseria gonorrhoeae: correlation of alterations in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV with antimicrobial susceptibility profiles.

Deguchi, T, Yasuda, M, Nakano, M, Ozeki, S, Ezaki, T, Saito, I, ...

Fifty-five clinical strains of Neisseria gonorrhoeae were examined for mutations in the gyrA and parC genes and for antimicrobial susceptibility profiles. The MICs of quinolones for 31 strains with...

Uncommon occurrence of mutations in the gyrB gene associated with quinolone resistance in clinical isolates of Neisseria gonorrhoeae.

Deguchi, T, Yasuda, M, Nakano, M, Ozeki, S, Kanematsu, E, Kawada, Y, ...

Incomplete transformation of rat cells by a deletion mutant of adenovirus type 5.

Shiroki, K, Maruyama, K, Saito, I, Fukui, Y, Shimojo, H

Rat 3Y1 cells were infected with adenovirus type 5 (Ad5) wild type, dl312 (deletion of 902 base pairs between 1.5-4.5 map units), and dl313 (deletion of 2,350 base pairs between 3.5-10.5 map units)....

Soluble CD14 mediates lipopolysaccharide-induced intercellular adhesion molecule 1 expression in cultured human gingival fibroblasts.

Hayashi, J, Masaka, T, Saito, I, Ishikawa, I

Intercellular adhesion molecule 1 (ICAM-1) is involved in the accumulation and activation of leukocytes in inflammatory diseases such as periodontitis. As reported previously, ICAM-1 is up-regulated...

Effective prevention of thrombocytopenia in mice using adenovirus-mediated transfer of HST-1 (FGF-4) gene.

Konishi, H, Ochiya, T, Sakamoto, H, Tsukamoto, M, Saito, I, Muto, T, ...

HST-1 (FGF-4) gene product is a member of the fibroblast growth factor family with a signal peptide and plays a crucial role in limb development. We showed previously that an intraperitoneal...

Nitric oxide production and inducible nitric oxide synthase expression in inflammatory arthritides.

Sakurai, H, Kohsaka, H, Liu, M F, Higashiyama, H, Hirata, Y, Kanno, K, ...

In this study, we have identified the source of nitric oxide (NO) produced in the human inflammatory joints by analyzing expression of inducible NO synthase. In ex vivo organ cultures, both...

Renal dehydropeptidase-I stability of LJC 10,627, a new carbapenem antibiotic.

Hikida, M, Kawashima, K, Nishiki, K, Furukawa, Y, Nishizawa, K, Saito, I, ...

LJC 10,627 is a new parenteral carbapenem antibiotic. LJC 10,627 stability against human renal dehydropeptidase-I was compared with that of imipenem. Hydrolysis of this compound was not detectable by...

Gene targeting with a replication-defective adenovirus vector.

Fujita, A, Sakagami, K, Kanegae, Y, Saito, I, Kobayashi, I

Wide application of the gene-targeting technique has been hampered by its low level of efficiency. A replication-defective adenovirus vector was used for efficient delivery of donor DNA in order to...

Comparison among performances of a ligase chain reaction-based assay and two enzyme immunoassays in detecting Chlamydia trachomatis in urine specimens from men with nongonococcal urethritis.

Deguchi, T, Yasuda, M, Uno, M, Tada, K, Iwata, H, Komeda, H, ...

We evaluated the performances of a ligase chain reaction (LCR)-based assay and two enzyme immunoassays (Chlamydiazyme and IDEIA) in the detection of Chlamydia trachomatis in urine specimens. We...

Rapid detection of point mutations of the Neisseria gonorrhoeae gyrA gene associated with decreased susceptibilities to quinolones.

Deguchi, T, Yasuda, M, Nakano, M, Ozeki, S, Ezaki, T, Maeda, S, ...

Mutations in the gyrA gene resulting in amino acid changes at Ser-91 and Asp-95 are significantly associated with decreased susceptibilities to quinolones in Neisseria gonorrhoeae. To detect these...

Rapid screening of point mutations of the Neisseria gonorrhoeae parC gene associated with resistance to quinolones.

Deguchi, T, Yasuda, M, Nakano, M, Kanematsu, E, Ozeki, S, Nishino, Y, ...

To detect quinolone resistance-associated mutations within the Asp-86, Ser-87, Ser-88, and Glu-91 codons of the Neisseria gonorrhoeae parC gene, we developed a rapid and simple assay based on...

Expression of processed envelope protein of hepatitis C virus in mammalian and insect cells.

Matsuura, Y, Harada, S, Suzuki, R, Watanabe, Y, Inoue, Y, Saito, I, ...

The putative envelope protein of hepatitis C virus (HCV) was expressed in insect cells by using a baculovirus expression vector and in monkey COS cells under the control of exogenous promoters. The...

Expression of processed core protein of hepatitis C virus in mammalian cells.

Harada, S, Watanabe, Y, Takeuchi, K, Suzuki, T, Katayama, T, Takebe, Y, ...

A structural protein of hepatitis C virus (HCV) was expressed in monkey COS cells under the control of an exogenous promoter, and a protein of 22 kDa was identified by immunoblot analysis. This...

Novel RNA family structure of hepatitis B virus expressed in human cells, using a helper-free adenovirus vector.

Saito, I, Oya, Y, Shimojo, H

Ad5-HBL is a type 5 adenovirus bearing the large BglII fragment (2.8 kilobases; 87% of the total genome) of hepatitis B virus (HBV), subtype adr. Eight HBV RNAs expressed in HeLa cells infected with...

Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end of its genome.

Saito, I, Oya, Y, Yamamoto, K, Yuasa, T, Shimojo, H

A novel helper-free adenovirus type 5 (Ad5) vector system, which utilizes a cloning site 0.2 kilobase (kb) from the right end of the genome, has been developed. To construct a nondefective Ad5...

Isolation of a nondefective recombinant between adenovirus type 5 and early region 1A of adenovirus type 12.

Shiroki, K, Saito, I, Maruyama, K, Shimojo, H

A nondefective recombinant between adenovirus type 5 (Ad5) and type 12 (Ad12), rc-1 (Ad5 dl312, carrying the Ad12 E1A gene), was isolated from hamster cell foci transformed by a defective...

Isolation of transformation-defective, replication-nondefective early region 1B mutants of adenovirus 12.

Fukui, Y, Saito, I, Shiroki, K, Shimojo, H

We isolated three adenovirus 12 early region 1B mutants (in205B, in205C, and dl205) by ligation of the cleaved DNA-protein complex and transfection of human embryo kidney cells with the ligation...

Characterization of a host range mutant of human adenovirus 12 defective in early region 1B.

Fukui, Y, Shiroki, K, Saito, I, Shimojo, H

We isolated an adenovirus 12 early region 1B mutant (in206B) by ligation of the cleaved DNA-protein complex and transfection of human embryo kidney cells with the ligation products. By deduction from...

Expression of the E4 gene is required for establishment of soft-agar colony-forming rat cell lines transformed by the adenovirus 12 E1 gene.

Shiroki, K, Hashimoto, S, Saito, I, Fukui, Y, Fukui, Y, Kato, H, ...

Rat 3Y1 cells were transfected with recombinant gARC ( pSV2gpt carrying the adenovirus 12 early region 1 [E1] gene), and focus formation was observed in monolayer cultures after culture of cells in...

Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector.

Oda, K, Kato, H, Saito, I, Sugano, S, Maruyama, K, Masuda, M, ...

Simian virus 40 (SV40) recombinants carrying the adenovirus type 12 E1A gene were constructed. The SV40 expression vector was constructed by removing most of the VP1 gene and an internal part of the...

Hyperproduction of adenovirus type 12 E1B gene product in monkey cells, using a simian virus 40 vector.

Oda, K, Fukui, Y, Saito, I, Masuda, M, Shiroki, K, Shimojo, H

Simian virus 40 recombinant DNAs carrying the adenovirus type 12 E1B gene were constructed, propagated, and packaged in monkey cells. Monkey cells infected with the resulting virus stocks...

Expression of adenovirus type 12 early region 1 in KB cells transformed by recombinants containing the gene.

Shiroki, K, Saito, I, Maruyama, K, Fukui, Y, Imatani, Y, Oda, K I, ...

The adenovirus type 12 (Ad12) early region 1 (E1) gene was introduced into KB cells by using a dominant selection vector, pSV2-gpt, and over 80 Gpt+ KB cell clones were established. Three types of...

Dependence of tumor-forming capacities of cells transformed by recombinants between adenovirus types 5 and 12 on expression of early region 1.

Shiroki, K, Maruyama, K, Saito, I, Fukui, Y, Yazaki, K, Shimojo, H

Recombinants between an adenovirus type 5 (Ad5) deletion mutant and the Ad12 DNA fragment containing early region 1 (E1) were isolated from cells cotransfected with the EcoRI-C fragment of Ad12 DNA...

Discrimination of hepatitis C virus in liver tissues from different patients with hepatocellular carcinomas by direct nucleotide sequencing of amplified cDNA of the viral genome.

Chou, W H, Yoneyama, T, Takeuchi, K, Harada, H, Saito, I, Miyamura, T

We investigated the presence of the hepatitis C virus (HCV) genome in liver tissues of eight different patients with hepatocellular carcinoma by using the reverse transcriptase polymerase chain...

Detection and mapping of spliced RNA from a human hepatoma cell line transfected with the hepatitis B virus genome.

Suzuki, T, Masui, N, Kajino, K, Saito, I, Miyamura, T

HepG2 cells, known to support the replication and virion formation of hepatitis B virus (HBV), were transfected with a cosmid constructed to contain 12 tandem head-to-tail repeats of the HBV genome...

Effects of cytokines and periodontopathic bacteria on the leukocyte function-associated antigen 1/intercellular adhesion molecule 1 pathway in gingival fibroblasts in adult periodontitis.

Hayashi, J, Saito, I, Ishikawa, I, Miyasaka, N

We investigated the effects of inflammatory cytokines and periodontopathic bacteria on expression of intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1, and E-selectin...

Enhancement of retrovirus-mediated gene transduction efficiency by transient overexpression of the amphotropic receptor, GLVR-2.

Yamaguchi, S, Wakimoto, H, Yoshida, Y, Kanegae, Y, Saito, I, Aoyagi, M, ...

Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase.

Kanegae, Y, Lee, G, Sato, Y, Tanaka, M, Nakai, M, Sakaki, T, ...

A recombinant adenovirus (Ad) expressing Cre recombinase derived from bacteriophage P1 was constructed. To assay the Cre activity in mammalian cells, another recombinant Ad bearing an...

Nucleotide sequence of the hepatitis C virus genome from a patient negative for anti-HCV by the first generation antibody assay.

Sarashina, T, Sakurai, T, Watanabe, Y, Kashima, K, Suzuki, T, Chiba, J, ...

A new procedure for determining thymine residues in DNA sequencing. Photoinduced cleavage of DNA fragments in the presence of spermine.

Saito, I, Sugiyama, H, Matsuura, T, Ueda, K, Komano, T

A new procedure for T specific cleavage of DNA fragments utilizing photoreaction with spermine has been described. Irradiation of 3'-[32P]-end-labeled DNA fragments for 10-20 min with a germicidal...

Nucleotide sequence of core and envelope genes of the hepatitis C virus genome derived directly from human healthy carriers.

Takeuchi, K, Kubo, Y, Boonmar, S, Watanabe, Y, Katayama, T, Choo, Q L, ...

A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non-A, non-B hepatitis in Japan.

Kubo, Y, Takeuchi, K, Boonmar, S, Katayama, T, Choo, Q L, Kuo, G, ...

Recently, a cDNA from the hepatitis C virus (HCV) RNA genome has been isolated in the USA from a chronically infected chimpanzee. In order to isolate HCV cDNA derived from human material, RNA was...

Evolution and stability of chromosomal DNA coamplified with the CAD gene.

Saito, I, Groves, R, Giulotto, E, Rolfe, M, Stark, G R

We have compared clones of Syrian hamster cells selected for the first amplification of the CAD gene with clones selected for further amplification. The large domain amplified initially was not...

Preferential amplification of rearranged sequences near amplified adenylate deaminase genes.

Debatisse, M, Saito, I, Buttin, G, Stark, G R

In a previous study of three independent families of mutants selected for overproduction of adenylate deaminase (AMPD), we were not able to isolate a cDNA probe for the gene and so could not...

Charomids: cosmid vectors for efficient cloning and mapping of large or small restriction fragments.

Saito, I, Stark, G R

Charomids are cosmid vectors up to 52 kilobases (kb) long, bearing 1-23 copies of a 2-kb spacer fragment linked in head-to-tail tandem arrays. Like cosmids and lambda phage, charomids can be packaged...

Retrovirus in salivary glands from patients with Sjögren's syndrome.

Yamano, S, Renard, J N, Mizuno, F, Narita, Y, Uchida, Y, Higashiyama, H, ...

AIMS: To investigate the possibility of an immune response to retroviral antigens or of detecting retrovirus in Sjögren's syndrome. METHODS: Retroviruses were sought in labial salivary glands and...

Possible involvement of IL-12 expression by Epstein-Barr virus in Sjögren syndrome.

Horiuchi, M, Yamano, S, Inoue, H, Ishii, J, Nagata, Y, Adachi, H, ...

AIM: To determine the correlation between interleukin 12 (IL-12) expression and Epstein-Barr virus (EBV) in Sjögren syndrome. METHODS: Indirect immunohistochemical technique, enzyme linked...

Local expression of immunoregulatory IL-12p40 gene prolonged syngeneic islet graft survival in diabetic NOD mice.

Yasuda, H, Nagata, M, Arisawa, K, Yoshida, R, Fujihira, K, Okamoto, N, ...

Local production of immunosuppressive cytokines will be one of the most suitable therapeutic strategies against organ-specific autoimmune diabetes. To establish such a new therapy, we constructed...

Structure of DNA formed in the first step of CAD gene amplification.

Giulotto, E, Saito, I, Stark, G R

Thirty-three independent mutant cell lines were selected in single steps for resistance to low concentrations of N-(phosphonacetyl)-L-aspartate and the structure of their amplified DNA was probed,...

Structural studies of a stable parallel-stranded DNA duplex incorporating isoguanine:cytosine and isocytosine:guanine basepairs by nuclear magnetic resonance spectroscopy.

Yang, X L, Sugiyama, H, Ikeda, S, Saito, I, Wang, A H

Isoguanine (2-hydroxyladenine) is a product of oxidative damage to DNA and has been shown to cause mutation. It is also a potent inducer of parallel-stranded DNA duplex structure. The structure of...

Cytokine regulation on the synthesis of nitric oxide in vivo by chronically infected human polymorphonuclear leucocytes.

Takeichi, O, Saito, I, Okamoto, Y, Tsurumachi, T, Saito, T

To determine if nitric oxide (NO) is produced by chronically infected human polymorphonuclear leucocytes (PMNs) in vivo, inflamed exudates (periapical exudates: PE) collected from periapical...

Induction of intercellular adhesion molecule-1 in human nasal epithelial cells during respiratory syncytial virus infection.

Matsuzaki, Z, Okamoto, Y, Sarashina, N, Ito, E, Togawa, K, Saito, I

The effects of infection with respiratory syncytial virus (RSV) on expression of intercellular adhesion molecule (ICAM)-1 was determined in vitro in nasal epithelial cell cultures. Functional...

Augmented expression of inflammatory cytokines and adhesion molecules in accelerated nodulosis during methotrexate therapy.

Miyasaka, N, Saito, I, Uemura, T, Kashiwazaki, S

Treatment of dry eye by autologous serum application in Sjögren's syndrome

Tsubota, K., Goto, E., Fujita, H., Ono, M., Inoue, H., Saito, I., ...

AIM—To evaluate the efficacy of autologous serum application for the treatment of dry eye in Sjögren's syndrome.
METHODS—The stability of essential components (EGF, vitamin A, and TGF-β) in...

Juxtaoral organ of Chievitz presenting clinically as a tumour

Ide, F, Mishima, K, Saito, I

An extremely rare hamartomatous lesion of the juxtaoral organ of Chievitz (JOOC) in a 63 year old man is reported. The tumour appeared as a large mass in the infratemporal fossa with associated...

Diverse T cell receptor beta gene usage by infiltrating T cells in the lacrimal glands of Sjögren's syndrome.

Mizushima, N, Kohsaka, H, Tsubota, K, Saito, I, Miyasaka, N

Sjögren's syndrome (SS) is an autoimmune disease characterized by T cell infiltration into the salivary and lacrimal glands (LG). Previous studies on T cell receptor (TCR) usage in the minor...

Measurement of IL-4 in tears of patients with seasonal allergic conjunctivitis and vernal keratoconjunctivitis.

Fujishima, H, Takeuchi, T, Shinozaki, N, Saito, I, Tsubota, K

To elucidate the mechanism of ocular surface allergic disease, we focused on IL-4, which is one of the key factors in regulating IgE production, and thus determined the concentration of IL-4 in...

High incidence of autoimmune dacryoadenitis in male non-obese diabetic (NOD) mice depending on sex steroid

TAKAHASHI, M, ISHIMARU, N, YANAGI, K, HANEJI, N, SAITO, I, HAYASHI, Y

The NOD mouse develops spontaneous autoimmune lesions in the lacrimal and salivary glands, besides a well characterized T cell-mediated autoimmune pancreatic β cell lesion. We report unique...

Analysis of T cell receptor Vβ usage in the autoimmune sialadenitis of non-obese diabetic (NOD) mice

YANAGI, K, HANEJI, N, ISHIMARU, N, SAITO, I, HAYASHI, Y

The NOD mouse develops spontaneous autoimmune sialadenitis besides a well characterized T cell-mediated autoimmune insulitis. We used reverse transcriptase-polymerase chain reaction (RT-PCR) to...

Pirfenidone induces intercellular adhesion molecule-1 (ICAM-1) down-regulation on cultured human synovial fibroblasts

Kaneko, M, Inoue, H, Nakazawa, R, Azuma, N, Suzuki, M, Yamauchi, S, ...

Pirfenidone has been shown to modify some cytokine regulatory actions and inhibit fibroblast biochemical reactions resulting in inhibition of proliferation and collagen matrix synthesis by...

Treatment with anti-CD86 costimulatory molecule prevents the autoimmune lesions in murine Sjögren's syndrome (SS) through up-regulated Th2 response

Saegusa, K, Ishimaru, N, Yanagi, K, Haneji, N, Nishino, M, Azuma, M, ...

Intraperitoneal administration with anti-CD86 (B7.2) MoAb into the murine model for primary SS in NFS/sld mutant mice resulted in dramatically inhibitory effects on the development of autoimmune...

Characteristics of immunity induced by viral antigen or conferred by antibody via different administration routes

MATSUOKA, T, OKAMOTO, Y, MATSUZAKI, Z, ENDO, S, ITO, E, TSUTSUMI, H, ...

The characteristics of the immunity induced by viral antigens or conferred by antiviral antibody via different routes of administration were evaluated comparatively. C57BL/6 mice were immunized via...

Determination of urinary 2,5-hexanedione concentration by an improved analytical method as an index of exposure to n-hexane.

Saito, I, Shibata, E, Huang, J, Hisanaga, N, Ono, Y, Takeuchi, Y

2,5-Hexanedione is a main metabolite of n-hexane and is considered as the cause of n-hexane polyneuropathy. Therefore, it is useful to measure 2,5-hexanedione for biological monitoring of exposure to...

Shwachman syndrome associated with de novo reciprocal translocation t(6;12)(q16.2;q21.2).

Masuno, M, Imaizumi, K, Nishimura, G, Nakamura, M, Saito, I, Akagi, K, ...

We describe a de novo apparently balanced reciprocal translocation t(6;12)(q16.2; q21.2) in an 18 month old girl with Shwachman syndrome, characterised by exocrine pancreatic insufficiency and bone...

Notch3 gene polymorphism and ischaemic cerebrovascular disease

Ito, D, Tanahashi, N, Murata, M, Sato, H, Saito, I, Watanabe, K, ...

Amelioration of lacrimal gland inflammation by oral administration of K-13182 in Sjögren's syndrome model mice

Nishiyama, T, Mishima, K, Obara, K, Inoue, H, Doi, T, Kondo, S, ...

Regulation of the adhesion of mononuclear cells to endothelial cells is considered to be a critical step for the treatment of inflammatory diseases, including autoimmune diseases. K-13182 was...

Ganglioneuromatous hamartoma of the tongue

Ide, F, Obara, K, Mishima, K, Saito, I