Gassmann, Reto, Essex, Anthony, Hu, Jia-Sheng, Maddox, Paul S., Motegi, Fumio, Sugimoto, Asako, ...
Chromosome segregation requires stable bipolar attachments of spindle microtubules to kinetochores. The dynein/dynactin motor complex localizes transiently to kinetochores and is implicated in...
Cheeseman, Iain M., Niessen, Sherry, Anderson, Scott, Hyndman, Francie, Yates, John R., Oegema, Karen, ...
Kinetochores play an essential role in chromosome segregation by forming dynamic connections with spindle microtubules. Here, we identify a set of 10 copurifying kinetochore proteins from...
Enquist-Newman, Maria, Cheeseman, Iain M., Van Goor, David, Drubin, David G., Meluh, Pamela B., Barnes, Georjana
We showed recently that a complex between Duo1p and Dam1p is required for both spindle integrity and kinetochore function in the budding yeast Saccharomyces cerevisiae. To extend our understanding of...
Kinetochore Protein Interactions and their Regulation by the Aurora Kinase Ipl1pD⃞
Shang, Ching, Hazbun, Tony R., Cheeseman, Iain M., Aranda, Jennifer, Fields, Stanley, Drubin, David G., ...
Although there has been a recent explosion in the identification of budding yeast kinetochore components, the physical interactions that underlie kinetochore function remain obscure. To better...
Cheeseman, Iain M., Niessen, Sherry, Anderson, Scott, Hyndman, Francie, Yates, John R., Oegema, Karen, ...
Kinetochores play an essential role in chromosome segregation by forming dynamic connections with spindle microtubules. Here, we identify a set of 10 copurifying kinetochore proteins from...
Enquist-Newman, Maria, Cheeseman, Iain M., Van Goor, David, Drubin, David G., Meluh, Pamela B., Barnes, Georjana
We showed recently that a complex between Duo1p and Dam1p is required for both spindle integrity and kinetochore function in the budding yeast Saccharomyces cerevisiae. To extend our understanding of...
Kinetochore Protein Interactions and their Regulation by the Aurora Kinase Ipl1pD⃞
Shang, Ching, Hazbun, Tony R., Cheeseman, Iain M., Aranda, Jennifer, Fields, Stanley, Drubin, David G., ...
Although there has been a recent explosion in the identification of budding yeast kinetochore components, the physical interactions that underlie kinetochore function remain obscure. To better...
Cheeseman, Iain M., Niessen, Sherry, Anderson, Scott, Hyndman, Francie, Yates, John R., Oegema, Karen, ...
Kinetochores play an essential role in chromosome segregation by forming dynamic connections with spindle microtubules. Here, we identify a set of 10 copurifying kinetochore proteins from...
The human Mis12 complex is required for kinetochore assembly and proper chromosome segregation
Kline, Susan L., Cheeseman, Iain M., Hori, Tetsuya, Fukagawa, Tatsuo, Desai, Arshad
During cell division, kinetochores form the primary chromosomal attachment sites for spindle microtubules. We previously identified a network of 10 interacting kinetochore proteins conserved between...
KNL1 and the CENP-H/I/K Complex Coordinately Direct Kinetochore Assembly in Vertebrates
Cheeseman, Iain M., Hori, Tetsuya, Fukagawa, Tatsuo, Desai, Arshad
Chromosome segregation during mitosis requires the assembly of a large proteinaceous structure termed the kinetochore. In Caenorhabditis elegans, KNL-1 is required to target multiple outer...
Saccharomyces cerevisiae Duo1p and Dam1p, Novel Proteins Involved in Mitotic Spindle Function
Hofmann, Christian, Cheeseman, Iain M., Goode, Bruce L., McDonald, Kent L., Barnes, Georjana, Drubin, David G.
In this paper, we describe the identification and characterization of two novel and essential mitotic spindle proteins, Duo1p and Dam1p. Duo1p was isolated because its overexpression caused defects...
Kang, Jung-seog, Cheeseman, Iain M., Kallstrom, George, Velmurugan, Soundarapandian, Barnes, Georjana, Chan, Clarence S.M.
We have shown previously that Ipl1 and Sli15 are required for chromosome segregation in Saccharomyces cerevisiae. Sli15 associates directly with the Ipl1 protein kinase and these two proteins...
Architecture of the budding yeast kinetochore reveals a conserved molecular core
Westermann, Stefan, Cheeseman, Iain M., Anderson, Scott, Yates, John R., Drubin, David G., Barnes, Georjana
How kinetochore proteins are organized to connect chromosomes to spindle microtubules, and whether any structural and organizational themes are common to kinetochores from distantly related...
Mitotic Spindle Integrity and Kinetochore Function Linked by the Duo1p/Dam1p Complex
Cheeseman, Iain M., Enquist-Newman, Maria, Müller-Reichert, Thomas, Drubin, David G., Barnes, Georjana
Duo1p and Dam1p were previously identified as spindle proteins in the budding yeast, Saccharomyces cerevisiae. Here, analyses of a diverse collection of duo1 and dam1 alleles were used to develop a...
Cheeseman, Iain M., Drubin, David G., Barnes, Georjana
Although the budding yeast centromere is extremely short (125 bp) compared to those of other eukaryotes, the kinetochore that assembles on this DNA displays a rich molecular complexity. Here, we...
Implication of a novel multiprotein Dam1p complex in outer kinetochore function
Cheeseman, Iain M., Brew, Christine, Wolyniak, Michael, Desai, Arshad, Anderson, Scott, Muster, Nemone, ...
Dam1p, Duo1p, and Dad1p can associate with each other physically and are required for both spindle integrity and kinetochore function in budding yeast. Here, we present our purification from yeast...
Gassmann, Reto, Essex, Anthony, Hu, Jia-Sheng, Maddox, Paul S., Motegi, Fumio, Sugimoto, Asako, ...
Chromosome segregation requires stable bipolar attachments of spindle microtubules to kinetochores. The dynein/dynactin motor complex localizes transiently to kinetochores and is implicated in...
Orientation and structure of the Ndc80 complex on the microtubule lattice
Wilson-Kubalek, Elizabeth M., Cheeseman, Iain M., Yoshioka, Craig, Desai, Arshad, Milligan, Ronald A.
The four-subunit Ndc80 complex, comprised of Ndc80/Nuf2 and Spc24/Spc25 dimers, directly connects kinetochores to spindle microtubules. The complex is anchored to the kinetochore at the Spc24/25 end,...