Iannis Aifantis

Efficiency of RNA Interference in the Mouse Hematopoietic System Varies between Cell Types and Developmental Stages

Oberdoerffer, Philipp, Kanellopoulou, Chryssa, Heissmeyer, Vigo, Paeper, Corinna, Borowski, Christine, Aifantis, Iannis, ...

RNA interference (RNAi) is a naturally occurring posttranscriptional gene-silencing mechanism that has been adapted as a genetic tool for loss-of-function studies of a variety of organisms. It is...

A multistep adhesion cascade for lymphoid progenitor cell homing to the thymus

Scimone, M. Lucila, Aifantis, Iannis, Apostolou, Irina, Von Boehmer, Harald, Von Andrian, Ulrich H.

Homing of bone marrow (BM)-derived progenitors to the thymus is essential for T cell development. We have previously reported that two subpopulations of common lymphoid progenitors, CLP-1 and CLP-2,...

Efficiency of RNA Interference in the Mouse Hematopoietic System Varies between Cell Types and Developmental Stages

Oberdoerffer, Philipp, Kanellopoulou, Chryssa, Heissmeyer, Vigo, Paeper, Corinna, Borowski, Christine, Aifantis, Iannis, ...

RNA interference (RNAi) is a naturally occurring posttranscriptional gene-silencing mechanism that has been adapted as a genetic tool for loss-of-function studies of a variety of organisms. It is...

A multistep adhesion cascade for lymphoid progenitor cell homing to the thymus

Scimone, M. Lucila, Aifantis, Iannis, Apostolou, Irina, Von Boehmer, Harald, Von Andrian, Ulrich H.

Homing of bone marrow (BM)-derived progenitors to the thymus is essential for T cell development. We have previously reported that two subpopulations of common lymphoid progenitors, CLP-1 and CLP-2,...

Notch1 promotes survival of E2A-deficient T cell lymphomas through pre–T cell receptor–dependent and –independent mechanisms

Reschly, Erica J., Spaulding, Christina, Vilimas, Tomas, Graham, W. Vallen, Brumbaugh, Rachel L., Aifantis, Iannis, ...

Loss of E2A transcription factor activity or activation of the intracellular form of Notch1 (ICN) leads to the development of leukemia or lymphoma in humans or mice, respectively. Current models...

The Eδ enhancer controls the generation of CD4−CD8− αβTCR-expressing T cells that can give rise to different lineages of αβ T cells

Aifantis, Iannis, Bassing, Craig H., Garbe, Annette I., Sawai, Katie, Alt, Frederick W., Von Boehmer, Harald

It is well established that the pre–T cell receptor for antigen (TCR) is responsible for efficient expansion and differentiation of thymocytes with productive TCRβ rearrangements. However, Ptcra-...

The SCFFBW7 ubiquitin ligase complex as a tumor suppressor in T cell leukemia

Thompson, Benjamin J., Buonamici, Silvia, Sulis, Maria Luisa, Palomero, Teresa, Vilimas, Tomas, Basso, Giuseppe, ...

Recent studies have shown that activating mutations of NOTCH1 are responsible for the majority of T cell acute lymphoblastic leukemia (T-ALL) cases. Most of these mutations truncate its C-terminal...

The BCL2A1 gene as a pre–T cell receptor–induced regulator of thymocyte survival

Mandal, Malay, Borowski, Christine, Palomero, Teresa, Ferrando, Adolfo A., Oberdoerffer, Philipp, Meng, Fanyong, ...

The pre–T cell receptor (TCR) is expressed early during T cell development and imposes a tight selection for differentiating T cell progenitors. Pre-TCR–expressing cells are selected to survive...

Pre-TCRα and TCRα Are Not Interchangeable Partners of TCRβ during T Lymphocyte Development

Borowski, Christine, Li, Xiaoyan, Aifantis, Iannis, Gounari, Fotini, Von Boehmer, Harald

In contrast with the αβ T cell receptor (TCR), the pre-TCR spontaneously segregates to membrane rafts from where it signals in a cell-autonomous fashion. The disparate behaviors of these two...

The Common Cytokine Receptor γ Chain and the Pre-T Cell Receptor Provide Independent but Critically Overlapping Signals in Early α/β T Cell Development

Di Santo, James P., Aifantis, Iannis, Rosmaraki, Eleftheria, Garcia, Corinne, Feinberg, Jacqueline, Fehling, Hans Jörg, ...

Intracellular signals emanating from cytokine and antigen receptors are integrated during the process of intrathymic development. Still, the relative contributions of cytokine receptor signaling to...

Early T Cell Receptor β Gene Expression Is Regulated by the Pre-T Cell Receptor–CD3 Complex

Aifantis, Iannis, Feinberg, Jacqueline, Fehling, Hans Jörg, Di Santo, James P., Von Boehmer, Harald

We have examined the question of whether there is an additional checkpoint in T cell development that regulates T cell receptor (TCR)-β expression in CD25+44− thymocytes by mechanisms that are...

Allelic Exclusion of the T Cell Receptor β Locus Requires the Sh2 Domain–Containing Leukocyte Protein (Slp)-76 Adaptor Protein

Aifantis, Iannis, Pivniouk, Vadim I., Gärtner, Frank, Feinberg, Jacqueline, Swat, Wojciech, Alt, Frederick W., ...

Signaling via the pre-T cell receptor (TCR) is required for the proliferative expansion and maturation of CD4−CD8− double-negative (DN) thymocytes into CD4+CD8+ double-positive (DP) cells and for...

Role of Different T Cell Receptors in the Development of Pre–T Cells

Buer, Jan, Aifantis, Iannis, DiSanto, James P., Fehling, Hans Joerg, Boehmer, Harald Von

The development of pre–T cells with productive TCR-β rearrangements can be mediated by each the pre–T cell receptor (pre-TCR), the TCR-αβ as well as the TCR-γδ, albeit by distinct...

Regulation of lymphocyte progenitor survival by the proapoptotic activities of Bim and Bid

Mandal, Malay, Crusio, Kelly M., Meng, Fanyong, Liu, Suqing, Kinsella, Marcus, Clark, Marcus R., ...

On their entry into the thymus, developing lymphocyte progenitors depend on signaling from the pre-T cell receptor (pre-TCR), which orchestrates differentiation, cell proliferation, and survival. The...