You, CongHui, Sekowska, Agnieszka, Francetic, Olivera, Martin-Verstraete, Isabelle, Wang, YiPing, Danchin, Antoine
Abstract Background All aerobically grown living cells are exposed to oxidative damage by reactive oxygen species (ROS). A major damage by ROS to proteins is caused by covalent modifications of...
Goelzer, Anne, Bekkal Brikci, Fadia, Martin-Verstraete, Isabelle, Noirot, Philippe, Bessières, Philippe, Aymerich, Stéphane, ...
Abstract Background Few genome-scale models of organisms focus on the regulatory networks and none of them integrates all known levels of regulation. In particular, the regulations involving...
André, Gaëlle, Even, Sergine, Putzer, Harald, Burguière, Pierre, Croux, Christian, Danchin, Antoine, ...
The ubiGmccBA operon of Clostridium acetobutylicum is involved in methionine to cysteine conversion. We showed that its expression is controlled by a complex regulatory system combining several...
BMC Systems Biology BioMed Central (2008)
Anne Goelzer, Fadia Bekkal Brikci, Isabelle Martin-verstraete, Stéphane Aymerich, Vincent Fromion
Research article Reconstruction and analysis of the genetic and metabolic regulatory networks of the central metabolism of Bacillus subtilis
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression
Galinier, Anne, Haiech, Jacques, Kilhoffer, Marie-Claude, Jaquinod, Michel, Stülke, Jörg, Deutscher, Josef, ...
Carbon catabolite repression (CCR) of several Bacillus subtilis catabolic genes is mediated by ATP-dependent phosphorylation of histidine-containing protein (HPr), a phosphocarrier protein of the...
Martin-Verstraete, Isabelle, Deutscher, Josef, Galinier, Anne
Carbon catabolite repression (CCR) of Bacillus subtilis catabolic genes is mediated by CcpA and in part by P-Ser–HPr. For certain operons, Crh, an HPr-like protein, is also implicated in CCR. In...
Catabolite Regulation of the pta Gene as Part of Carbon Flow Pathways in Bacillus subtilis
Presecan-Siedel, Elena, Galinier, Anne, Longin, Robert, Deutscher, Josef, Danchin, Antoine, Glaser, Philippe, ...
In Bacillus subtilis, the products of the pta and ackA genes, phosphotransacetylase and acetate kinase, play a crucial role in the production of acetate, one of the most abundant by-products of...
CotA of Bacillus subtilis Is a Copper-Dependent Laccase
Hullo, Marie-Françoise, Moszer, Ivan, Danchin, Antoine, Martin-Verstraete, Isabelle
The spore coat protein CotA of Bacillus subtilis displays similarities with multicopper oxidases, including manganese oxidases and laccases. B. subtilis is able to oxidize manganese, but neither CotA...
Guillouard, Isabelle, Auger, Sandrine, Hullo, Marie-Françoise, Chetouani, Farid, Danchin, Antoine, Martin-Verstraete, Isabelle
The way in which the genes involved in cysteine biosynthesis are regulated is poorly characterized in Bacillus subtilis. We showed that CysL (formerly YwfK), a LysR-type transcriptional regulator,...
Global Expression Profile of Bacillus subtilis Grown in the Presence of Sulfate or Methionine
Auger, Sandrine, Danchin, Antoine, Martin-Verstraete, Isabelle
DNA arrays were used to investigate the global transcriptional profile of Bacillus subtilis grown in the presence of sulfate or methionine as the sole sulfur source. The expression of at least 56...
Mutations lowering the phosphatase activity of HPr kinase/phosphatase switch off carbon metabolism
Monedero, Vicente, Poncet, Sandrine, Mijakovic, Ivan, Fieulaine, Sonia, Dossonnet, Valérie, Martin-Verstraete, Isabelle, ...
The oligomeric bifunctional HPr kinase/P-Ser-HPr phosphatase (HprK/P) regulates many metabolic functions in Gram-positive bacteria by phosphorylating the phosphocarrier protein HPr at Ser46. We...
Three Different Systems Participate in l-Cystine Uptake in Bacillus subtilis
Burguière, Pierre, Auger, Sandrine, Hullo, Marie-Françoise, Danchin, Antoine, Martin-Verstraete, Isabelle
The symporter YhcL and two ATP binding cassette transporters, YtmJKLMN and YckKJI, were shown to mediate l-cystine uptake in Bacillus subtilis. A triple ΔyhcL ΔytmJKLMN ΔyckK mutant was unable to...
Regulation of the Bacillus subtilis ytmI Operon, Involved in Sulfur Metabolism
Burguière, Pierre, Fert, Juliette, Guillouard, Isabelle, Auger, Sandrine, Danchin, Antoine, Martin-Verstraete, Isabelle
The YtlI regulator of Bacillus subtilis activates the transcription of the ytmI operon encoding an l-cystine ABC transporter, a riboflavin kinase, and proteins of unknown function. The expression of...
Global Control of Cysteine Metabolism by CymR in Bacillus subtilis†
Even, Sergine, Burguière, Pierre, Auger, Sandrine, Soutourina, Olga, Danchin, Antoine, Martin-Verstraete, Isabelle
YrzC has previously been identified as a repressor controlling ytmI expression via its regulation of YtlI activator synthesis in Bacillus subtilis. We identified YrzC as a master regulator of sulfur...
The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression
Galinier, Anne, Haiech, Jacques, Kilhoffer, Marie-Claude, Jaquinod, Michel, Stülke, Jörg, Deutscher, Josef, ...
Carbon catabolite repression (CCR) of several Bacillus subtilis catabolic genes is mediated by ATP-dependent phosphorylation of histidine-containing protein (HPr), a phosphocarrier protein of the...
Martin-Verstraete, Isabelle, Deutscher, Josef, Galinier, Anne
Carbon catabolite repression (CCR) of Bacillus subtilis catabolic genes is mediated by CcpA and in part by P-Ser–HPr. For certain operons, Crh, an HPr-like protein, is also implicated in CCR. In...
Catabolite Regulation of the pta Gene as Part of Carbon Flow Pathways in Bacillus subtilis
Presecan-Siedel, Elena, Galinier, Anne, Longin, Robert, Deutscher, Josef, Danchin, Antoine, Glaser, Philippe, ...
In Bacillus subtilis, the products of the pta and ackA genes, phosphotransacetylase and acetate kinase, play a crucial role in the production of acetate, one of the most abundant by-products of...
CotA of Bacillus subtilis Is a Copper-Dependent Laccase
Hullo, Marie-Françoise, Moszer, Ivan, Danchin, Antoine, Martin-Verstraete, Isabelle
The spore coat protein CotA of Bacillus subtilis displays similarities with multicopper oxidases, including manganese oxidases and laccases. B. subtilis is able to oxidize manganese, but neither CotA...
Guillouard, Isabelle, Auger, Sandrine, Hullo, Marie-Françoise, Chetouani, Farid, Danchin, Antoine, Martin-Verstraete, Isabelle
The way in which the genes involved in cysteine biosynthesis are regulated is poorly characterized in Bacillus subtilis. We showed that CysL (formerly YwfK), a LysR-type transcriptional regulator,...
Global Expression Profile of Bacillus subtilis Grown in the Presence of Sulfate or Methionine
Auger, Sandrine, Danchin, Antoine, Martin-Verstraete, Isabelle
DNA arrays were used to investigate the global transcriptional profile of Bacillus subtilis grown in the presence of sulfate or methionine as the sole sulfur source. The expression of at least 56...
Mutations lowering the phosphatase activity of HPr kinase/phosphatase switch off carbon metabolism
Monedero, Vicente, Poncet, Sandrine, Mijakovic, Ivan, Fieulaine, Sonia, Dossonnet, Valérie, Martin-Verstraete, Isabelle, ...
The oligomeric bifunctional HPr kinase/P-Ser-HPr phosphatase (HprK/P) regulates many metabolic functions in Gram-positive bacteria by phosphorylating the phosphocarrier protein HPr at Ser46. We...
Three Different Systems Participate in l-Cystine Uptake in Bacillus subtilis
Burguière, Pierre, Auger, Sandrine, Hullo, Marie-Françoise, Danchin, Antoine, Martin-Verstraete, Isabelle
The symporter YhcL and two ATP binding cassette transporters, YtmJKLMN and YckKJI, were shown to mediate l-cystine uptake in Bacillus subtilis. A triple ΔyhcL ΔytmJKLMN ΔyckK mutant was unable to...
Regulation of the Bacillus subtilis ytmI Operon, Involved in Sulfur Metabolism
Burguière, Pierre, Fert, Juliette, Guillouard, Isabelle, Auger, Sandrine, Danchin, Antoine, Martin-Verstraete, Isabelle
The YtlI regulator of Bacillus subtilis activates the transcription of the ytmI operon encoding an l-cystine ABC transporter, a riboflavin kinase, and proteins of unknown function. The expression of...
Global Control of Cysteine Metabolism by CymR in Bacillus subtilis†
Even, Sergine, Burguière, Pierre, Auger, Sandrine, Soutourina, Olga, Danchin, Antoine, Martin-Verstraete, Isabelle
YrzC has previously been identified as a repressor controlling ytmI expression via its regulation of YtlI activator synthesis in Bacillus subtilis. We identified YrzC as a master regulator of sulfur...
Conversion of Methionine to Cysteine in Bacillus subtilis and Its Regulation▿
Hullo, Marie-Françoise, Auger, Sandrine, Soutourina, Olga, Barzu, Octavian, Yvon, Mireille, Danchin, Antoine, ...
Bacillus subtilis can use methionine as the sole sulfur source, indicating an efficient conversion of methionine to cysteine. To characterize this pathway, the enzymatic activities of CysK, YrhA and...
Control of Methionine Synthesis and Uptake by MetR and Homocysteine in Streptococcus mutans▿
Sperandio, Brice, Gautier, Céline, McGovern, Stephen, Ehrlich, Dusko S., Renault, Pierre, Martin-Verstraete, Isabelle, ...
MetR (formerly Smu.1225), a regulator of the LysR family, controls key genes for methionine supply in Streptococcus mutans. An S. mutans metR mutant is unable to transport l-methionine and to grow in...
Goelzer, Anne, Bekkal Brikci, Fadia, Martin-Verstraete, Isabelle, Noirot, Philippe, Bessières, Philippe, Aymerich, Stéphane, ...
You, CongHui, Sekowska, Agnieszka, Francetic, Olivera, Martin-Verstraete, Isabelle, Wang, YiPing, Danchin, Antoine
André, Gaëlle, Even, Sergine, Putzer, Harald, Burguière, Pierre, Croux, Christian, Danchin, Antoine, ...
The ubiGmccBA operon of Clostridium acetobutylicum is involved in methionine to cysteine conversion. We showed that its expression is controlled by a complex regulatory system combining several...