Scientific Commons: J. A. Escobedo

Platelet-derived growth factor triggers translocation of the insulin-regulatable glucose transporter (type 4) predominantly through phosphatidylinositol 3-kinase binding sites on the receptor.

Kamohara, S, Hayashi, H, Todaka, M, Kanai, F, Ishii, K, Imanaka, T, ...

Insulin is the only known hormone which rapidly stimulates glucose uptake in target tissues, mainly by translocation to the cell surface of the intracellular insulin-regulatable glucose transporter...

Genetic immunization with adeno-associated virus vectors expressing herpes simplex virus type 2 glycoproteins B and D.

Manning, W C, Paliard, X, Zhou, S, Pat Bland, M, Lee, A Y, Hong, K, ...

Intramuscular injection of mice with an adeno-associated virus (AAV) vector expressing herpes simplex virus type 2 glycoprotein B led to the generation of both gB-specific major histocompatibility...

Identification of Tyr-397 as the primary site of tyrosine phosphorylation and pp60src association in the focal adhesion kinase, pp125FAK.

Eide, B L, Turck, C W, Escobedo, J A

A number of cellular processes, such as proliferation, differentiation, and transformation, are regulated by cell-extracellular matrix interactions. Previous studies have identified a novel tyrosine...

The interaction of small domains between the subunits of phosphatidylinositol 3-kinase determines enzyme activity.

Klippel, A, Escobedo, J A, Hirano, M, Williams, L T

Previous studies have suggested that the two subunits of phosphatidylinositol (PI) 3-kinase, p85 and p110, function as localizing and catalytic subunits, respectively. Using recombinant p85 and p110...

A phosphatidylinositol-3 kinase binds to platelet-derived growth factor receptors through a specific receptor sequence containing phosphotyrosine.

Escobedo, J A, Kaplan, D R, Kavanaugh, W M, Turck, C W, Williams, L T

Platelet-derived growth factor (PDGF) stimulates autophosphorylation of the PDGF receptor and association of the receptor with several cytoplasmic molecules, including phosphatidylinositol-3 kinase...

Platelet-derived growth factor receptor mediates activation of ras through different signaling pathways in different cell types.

Satoh, T, Fantl, W J, Escobedo, J A, Williams, L T, Kaziro, Y

A series of pieces of evidence have shown that Ras protein acts as a transducer of the platelet-derived growth factor (PDGF) receptor-mediated signaling pathway: (i) formation of Ras.GTP is detected...

A region of the 85-kilodalton (kDa) subunit of phosphatidylinositol 3-kinase binds the 110-kDa catalytic subunit in vivo.

Klippel, A, Escobedo, J A, Hu, Q, Williams, L T

Phosphatidylinositol (PI) 3-kinase is a heterodimer consisting of an 85-kDa subunit (p85) and 110-kDa subunit (p110). The 85-kDa noncatalytic subunit, which contains two Src homology 2 (SH2) domains,...

Mutations of the platelet-derived growth factor receptor that cause a loss of ligand-induced conformational change, subtle changes in kinase activity, and impaired ability to stimulate DNA synthesis.

Fantl, W J, Escobedo, J A, Williams, L T

The wild-type and two mitogenic-defective mutants of the type beta receptor for platelet-derived growth factor (PDGF) were expressed in Chinese hamster ovary cells. In the first mutant, delta Ki, 82...

Modification of the 85-kilodalton subunit of phosphatidylinositol-3 kinase in platelet-derived growth factor-stimulated cells.

Kavanaugh, W M, Klippel, A, Escobedo, J A, Williams, L T

The activated platelet-derived growth factor (PDGF) receptor physically associates with p85, a subunit of phosphatidylinositol-3 kinase. Although this interaction may activate...

Role of tyrosine kinase and membrane-spanning domains in signal transduction by the platelet-derived growth factor receptor.

Escobedo, J A, Barr, P J, Williams, L T

Three types of mutations were introduced into the platelet-derived growth factor (PDGF) receptor to cause a loss of PDGF-stimulated tyrosine kinase activity: (i) a point mutation of the ATP-binding...

The C-terminal SH2 domain of p85 accounts for the high affinity and specificity of the binding of phosphatidylinositol 3-kinase to phosphorylated platelet-derived growth factor beta receptor.

Klippel, A, Escobedo, J A, Fantl, W J, Williams, L T

Upon stimulation by its ligand, the platelet-derived growth factor (PDGF) receptor associates with the 85-kDa subunit of phosphatidylinositol (PI) 3-kinase. The 85-kDa protein (p85) contains two Src...

Cardiac myocyte hypertrophy is associated with c-myc protooncogene expression.

Starksen, N F, Simpson, P C, Bishopric, N, Coughlin, S R, Lee, W M, Escobedo, J A, ...

The mechanism of hormonally induced cell hypertrophy is unknown. Stimulation of cardiac myocytes by alpha 1-adrenergic agents, phorbol esters, and serum induces an increase in the cell size of...