Soluble E-Cadherin: An Early Marker of Severity in Acute Pancreatitis (2009)
A. Sewpaul, J. J. French, T. K. Khoo, M. Kernohan, J. A. Kirby, R. M. Charnley
Background/Aims. At present, there is no simple test for predicting severity in acute pancreatitis. We investigated the use of an assay of soluble E-cadherin (sE-cadherin). Methods. Concentrations of...
Soluble E-Cadherin: An Early Marker of Severity in Acute Pancreatitis (2009)
A. Sewpaul, J. J. French, T. K. Khoo, M. Kernohan, J. A. Kirby, R. M. Charnley
Background/Aims. At present, there is no simple test for predicting severity in acute pancreatitis. We investigated the use of an assay of soluble E-cadherin (sE-cadherin). Methods. Concentrations of...
Renal allograft rejection: examination of adhesion blockade by antilymphocyte antibody drugs (1993)
Kirby, J. A., Lin, Y., Browell, D. A., Clark, K., Shenton, B. K., Forsythe, J. L. R., ...
An assay was developed to investigate the binding of lymphocytes to cultured human renal epithelial cells. This binding was increased following lymphocyte activation by culture either with a...
Kirby, J. A., Forsythe, J. L. R., Simm, A., Proud, G., Taylor, R. M. R.
Human renal tissue was disaggregated by passage through a stainless-steel mesh and the resultant material was separated on the basis of particle size. The tubular fraction recovered from a 45 μm...
Ali, S, Palmer, A C, Fritchley, S J, Maley, Y, Kirby, J A
Chemokines interact with specific G-protein-coupled cell-surface receptors and with glycosaminoglycans (GAGs), such as heparan sulphate. Although chemokines often form multimers in solution, this...
Ali, S, Palmer, A C, Fritchley, S J, Maley, Y, Kirby, J A
Chemokines interact with specific G-protein-coupled cell-surface receptors and with glycosaminoglycans (GAGs), such as heparan sulphate. Although chemokines often form multimers in solution, this...
Cunningham, A C, Zhang, J G, Moy, J V, Ali, S, Kirby, J A
Human lung alveolar epithelial cells constitutively express class II major histocompatibility complex (MHC). Human lung microvascular endothelial and small airway epithelial cells can be induced to...
Douglas, M S, Ali, S, Rix, D A, Zhang, J G, Kirby, J A
Heparin is a polyanionic glycosaminoglycan (GAG) that can bind with high affinity to a range of cytokines including interferon-gamma (IFN-gamma) and members of the chemokine superfamily. This GAG...
Alloantigen presentation by B cells: analysis of the requirement for B-cell activation.
Wilson, J L, Cunningham, A C, Kirby, J A
This paper describes a model for investigation of the functional implications of B-cell activation for antigen presentation. Mixed lymphocyte cultures were used to assess the ability of freshly...
Regulation and function of adhesion molecule expression by human alveolar epithelial cells.
The role of major histocompatibility complex (MHC) and adhesion molecule expression by alveolar epithelium on the modulation of immune responses in the lung is not understood. We have developed...
Kirby, J A, Ikuta, S, Clark, K, Proud, G, Lennard, T W, Taylor, R M
Defined lines of primary human renal epithelial cells were established and their expression of class II major histocompatibility (MHC) antigens was up-regulated by culture with interferon-gamma...
Human endothelial cells: effect of TNF-alpha on peripheral blood mononuclear cell adhesion.
Ikuta, S, Kirby, J A, Shenton, B K, Givan, A L, Lennard, T W
Human umbilical vein endothelial cells (HUVEC) were cultured and treated for varying periods with a range of concentrations of tumour necrosis factor-alpha (TNF-alpha). After this treatment the...
Renal allograft rejection: possible involvement of lymphokine-activated killer cells.
Kirby, J A, Forsythe, J L, Proud, G, Taylor, R M
Human renal allograft tissue was recovered at transplant nephrectomy from three patients with irreversible loss of graft function. This tissue was disaggregated and separated into two fractions on...
Lin, Y, Proud, G, Taylor, R M, Kirby, J A
The potential of natural killer (NK) cells to contribute to renal allograft rejection was modelled by mixing NK cells with cultured renal epithelial cells. It was found that the renal cells were...
Demonstration of direct xenorecognition of porcine cells by human cytotoxic T lymphocytes.
Cunningham, A C, Butler, T J, Kirby, J A
It is not known whether human cytotoxic T cells can recognize porcine major histocompatibility antigens directly, or whether recognition occurs by co-operation with syngeneic human antigen-presenting...
Kirby, J A, Parfett, G J, Reader, J A, Pepper, J R
Single-lung transplantation in the rat has been shown to provide an effective model for the study of cellular events associated with allograft rejection. It is possible to recover sufficient viable...
Rat heterotopic heart transplantation: quantification and analysis of cell mediated cytotoxicity.
Kirby, J A, Reader, J A, Parfett, G J, Pepper, J R
Limiting dilution analysis was used to measure the frequency of PVG-reactive cytotoxic T lymphocytes (CTL) within the peripheral blood mononuclear cell population of Lewis rats before heterotopic...
Lin, Y, Kirby, J A, Clark, K, Shenton, B K, Forsythe, J L, Proud, G, ...
The interaction of graft-infiltrating immune cells with donor parenchymal cells is an important early event in allograft rejection. This binding is stabilized by interaction of antigen-independent...
Renal allograft rejection: expression and function of VCAM-1 on tubular epithelial cells.
Lin, Y, Kirby, J A, Browell, D A, Morley, A R, Shenton, B K, Proud, G, ...
The interaction between vascular cell adhesion molecule-1 (VCAM-1) and very late antigen-4 (VLA-4) is known to play an important role in stabilizing the adhesion of lymphocytes to endothelial cells....
Precursor frequency of donor-specific lymphocytes recovered from canine lung transplants.
Kirby, J A, Pepper, J R, Reader, J A, Corbishley, C M, Hudson, L
Dogs receiving left unilateral allo- or auto-grafts were treated with Cyclosporin A (Cy A) for 4 days. Thereafter allografted transplant recipients showed pulmonary pathology consistent with...
MACDONALD, P, PALMER, J, KIRBY, J A, JONES, D E J
A number of antigens implicated in the pathogenesis of autoimmune diseases including Sjogren's syndrome and systemic lupus erythematosus (SLE) are expressed aberrantly by apoptotic cells. It is also...
DOUGLAS, M S, RIX, D A, DARK, J H, TALBOT, D, KIRBY, J A
IFN-γ increases the potential immunogenicity of vascular endothelial cells by up-regulation of intercellular adhesion molecule-1 (ICAM-1) and class I MHC antigen expression and by induction of class...
PETTIT, S J, ALI, S, O'FLAHERTY, E, GRIFFITHS, T R L, NEAL, D E, KIRBY, J A
Transitional cell carcinomas (TCC) of the urinary bladder are known to express proteins which can yield potentially immunogenic peptide epitopes for expression in the context of cell surface class I...
Fritchley, S J, Kirby, J A, Ali, S
IFN-γ is a pleiotropic cytokine that is primarily involved in the regulation of immune cell activation and the development of tissue inflammation. It is capable of activating a range of non-immune...
Secretory autoantibodies in primary biliary cirrhosis (PBC)
Palmer, J M, Doshi, M, Kirby, J A, Yeaman, S J, Bassendine, M F, Jones, D E J
It is unclear how breakdown in immune tolerance to the ubiquitous self-antigen pyruvate dehydrogenase complex (PDC), seen in the autoimmune liver disease PBC, gives rise to tissue damage with such a...
Accelerating the induction of Fas-mediated T cell apoptosis: a strategy for transplant tolerance?
Carroll, H P, Ali, S, Kirby, J A
Acute allograft rejection is primarily a consequence of clonal expansion of donor-specific T cells with specificity for donor antigen. Immunosuppression current involves the administration of toxic...
Cresswell, J, Robertson, H, Neal, D E, Griffiths, T R L, Kirby, J A
The primary aim of this work was to survey normal urothelium and transitional cell carcinoma (TCC) for the presence of T lymphocytes expressing the intraepithelial, CD103+ phenotype. This antigen...
Monocyte Chemoattractant Proteins (MCPs) form a distinct, structurally-related subclass of CC chemokines. They are major chemoattractants for monocytes and T lymphocytes. The MCPs bind to specific...
The immunology of primary biliary cirrhosis: the end of the beginning?
PALMER, J M, KIRBY, J A, JONES, D E J
The chronic liver disease primary biliary cirrhosis (PBC) is characterised by autoreactive B-cell and T-cell responses directed against mitochondrial antigens. In recent years these responses have...
RIX, D A, DOUGLAS, M S, TALBOT, D, DARK, J H, KIRBY, J A
Heparan sulphate is a common glycosaminoglycan component of proteoglycans present on the luminal surface of vascular endothelium. It has been proposed that an important function of these molecules is...
Development of immunological assays to monitor pulmonary allograft rejection.
Cunningham, A C, Kirby, J A, Colquhoun, I W, Flecknell, P A, Ashcroft, T, Dark, J H
BACKGROUND--At present the diagnosis of pulmonary allograft rejection is made after examination of transbronchial biopsy specimens; this method is highly invasive. A study was performed to determine...
Regulation of T-cell apoptosis: a mixed lymphocyte reaction model
O'flaherty, E, Wong, W-K, Pettit, S J, Seymour, K, Ali, S, Kirby, J A
Despite the capacity for antigen-specific activation and rapid clonal expansion, homeostatic mechanisms ensure that the mature immune system contains a relatively stable number of T cells. In recent...
Role of the mucosal integrin αE(CD103)β7 in tissue-restricted cytotoxicity
Smyth, L J C, Kirby, J A, Cunningham, A C
The effectiveness of lung transplantation is marred by the relatively high incidence of rejection. The lung normally contains a large population of lymphocytes in contact with the airway epithelium,...
Soluble E-Cadherin: An Early Marker of Severity in Acute Pancreatitis
Sewpaul, A., French, J. J., Khoo, T. K., Kernohan, M., Kirby, J. A., Charnley, R. M.
Background/Aims. At present, there is no simple test for predicting severity in acute pancreatitis. We investigated the use of an assay of soluble E-cadherin (sE-cadherin). Methods. Concentrations of...
Mellor, P, Harvey, J R, Murphy, K J, Pye, D, O'Boyle, G, Lennard, T W J, ...
Expression of the chemokine receptor CXCR4 allows breast cancer cells to migrate towards specific metastatic target sites which constitutively express CXCL12. In this study, we determined whether...
Immune selection in neoplasia: towards a microevolutionary model of cancer development
Pettit, S J, Seymour, K, O'Flaherty, E, Kirby, J A
The dual properties of genetic instability and clonal expansion allow the development of a tumour to occur in a microevolutionary fashion. A broad range of pressures are exerted upon a tumour during...
T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy?
French, J J, Cresswell, J, Wong, W K, Seymour, K, Charnley, R M, Kirby, J A
Pancreatic cancer is an aggressive and potent disease, which is largely resistant to conventional forms of treatment. However, the discovery of antigens associated with pancreatic cancer cells has...