J. Andrew Alspaugh

Adenylyl Cyclase Functions Downstream of the Gα Protein Gpa1 and Controls Mating and Pathogenicity of Cryptococcus neoformans (2002)

Alspaugh, J. Andrew, Pukkila-Worley, Read, Harashima, Toshiaki, Cavallo, Lora M., Funnell, Deanna L., Cox, Gary M., ...

The signaling molecule cyclic AMP (cAMP) is a ubiquitous second messenger that enables cells to detect and respond to extracellular signals. cAMP is generated by the enzyme adenylyl cyclase, which is...

Cyclic AMP-Dependent Protein Kinase Controls Virulence of the Fungal Pathogen Cryptococcus neoformans

D'Souza, Cletus A., Alspaugh, J. Andrew, Yue, Changli, Harashima, Toshiaki, Cox, Gary M., Perfect, John R., ...

Cryptococcus neoformans is an opportunistic fungal pathogen that infects the human central nervous system. This pathogen elaborates two specialized virulence factors: the antioxidant melanin and an...

Adenylyl Cyclase Functions Downstream of the Gα Protein Gpa1 and Controls Mating and Pathogenicity of Cryptococcus neoformans

Alspaugh, J. Andrew, Pukkila-Worley, Read, Harashima, Toshiaki, Cavallo, Lora M., Funnell, Deanna, Cox, Gary M., ...

The signaling molecule cyclic AMP (cAMP) is a ubiquitous second messenger that enables cells to detect and respond to extracellular signals. cAMP is generated by the enzyme adenylyl cyclase, which is...

Cryptococcus neoformans mating and virulence are regulated by the G-protein α subunit GPA1 and cAMP

Alspaugh, J. Andrew, Perfect, John R., Heitman, Joseph

This study explores signal transduction pathways that function during mating and infection in the opportunistic, human fungal pathogen Cryptococcus neoformans. The gene encoding a G-protein α...

Transcriptional Network of Multiple Capsule and Melanin Genes Governed by the Cryptococcus neoformans Cyclic AMP Cascade

Pukkila-Worley, Read, Gerrald, Quincy D., Kraus, Peter R., Boily, Marie-Josée, Davis, Matthew J., Giles, Steven S., ...

Cryptococcus neoformans is an opportunistic human fungal pathogen that elaborates several virulence attributes, including a polysaccharide capsule and melanin pigments. A conserved Gα protein/cyclic...

Chromosomal Translocation and Segmental Duplication in Cryptococcus neoformans†

Fraser, James A., Huang, Johnny C., Pukkila-Worley, Read, Alspaugh, J. Andrew, Mitchell, Thomas G., Heitman, Joseph

Large chromosomal events such as translocations and segmental duplications enable rapid adaptation to new environments. Here we marshal genomic, genetic, meiotic mapping, and physical evidence to...

A Rac Homolog Functions Downstream of Ras1 To Control Hyphal Differentiation and High-Temperature Growth in the Pathogenic Fungus Cryptococcus neoformans

Vallim, Marcelo A., Nichols, Connie B., Fernandes, Larissa, Cramer, Kari L., Alspaugh, J. Andrew

The Cryptococcus neoformans Ras1 protein serves as a central regulator for several signaling pathways. Ras1 controls the induction of the mating pheromone response cascade as well as a distinct...

Transcription Factor Nrg1 Mediates Capsule Formation, Stress Response, and Pathogenesis in Cryptococcus neoformans

Cramer, Kari L., Gerrald, Quincy D., Nichols, Connie B., Price, Michael S., Alspaugh, J. Andrew

The Cryptococcus neoformans NRG1 gene was identified using gene microarrays to define putative transcription factor genes regulated by the cyclic AMP (cAMP) signal transduction pathway. Disruption of...

Cyclic AMP-Dependent Protein Kinase Controls Virulence of the Fungal Pathogen Cryptococcus neoformans

D'Souza, Cletus A., Alspaugh, J. Andrew, Yue, Changli, Harashima, Toshiaki, Cox, Gary M., Perfect, John R., ...

Cryptococcus neoformans is an opportunistic fungal pathogen that infects the human central nervous system. This pathogen elaborates two specialized virulence factors: the antioxidant melanin and an...

Adenylyl Cyclase Functions Downstream of the Gα Protein Gpa1 and Controls Mating and Pathogenicity of Cryptococcus neoformans

Alspaugh, J. Andrew, Pukkila-Worley, Read, Harashima, Toshiaki, Cavallo, Lora M., Funnell, Deanna, Cox, Gary M., ...

The signaling molecule cyclic AMP (cAMP) is a ubiquitous second messenger that enables cells to detect and respond to extracellular signals. cAMP is generated by the enzyme adenylyl cyclase, which is...

Cryptococcus neoformans mating and virulence are regulated by the G-protein α subunit GPA1 and cAMP

Alspaugh, J. Andrew, Perfect, John R., Heitman, Joseph

This study explores signal transduction pathways that function during mating and infection in the opportunistic, human fungal pathogen Cryptococcus neoformans. The gene encoding a G-protein α...

Transcriptional Network of Multiple Capsule and Melanin Genes Governed by the Cryptococcus neoformans Cyclic AMP Cascade

Pukkila-Worley, Read, Gerrald, Quincy D., Kraus, Peter R., Boily, Marie-Josée, Davis, Matthew J., Giles, Steven S., ...

Cryptococcus neoformans is an opportunistic human fungal pathogen that elaborates several virulence attributes, including a polysaccharide capsule and melanin pigments. A conserved Gα protein/cyclic...

Chromosomal Translocation and Segmental Duplication in Cryptococcus neoformans†

Fraser, James A., Huang, Johnny C., Pukkila-Worley, Read, Alspaugh, J. Andrew, Mitchell, Thomas G., Heitman, Joseph

Large chromosomal events such as translocations and segmental duplications enable rapid adaptation to new environments. Here we marshal genomic, genetic, meiotic mapping, and physical evidence to...

A Rac Homolog Functions Downstream of Ras1 To Control Hyphal Differentiation and High-Temperature Growth in the Pathogenic Fungus Cryptococcus neoformans

Vallim, Marcelo A., Nichols, Connie B., Fernandes, Larissa, Cramer, Kari L., Alspaugh, J. Andrew

The Cryptococcus neoformans Ras1 protein serves as a central regulator for several signaling pathways. Ras1 controls the induction of the mating pheromone response cascade as well as a distinct...

Transcription Factor Nrg1 Mediates Capsule Formation, Stress Response, and Pathogenesis in Cryptococcus neoformans

Cramer, Kari L., Gerrald, Quincy D., Nichols, Connie B., Price, Michael S., Alspaugh, J. Andrew

The Cryptococcus neoformans NRG1 gene was identified using gene microarrays to define putative transcription factor genes regulated by the cyclic AMP (cAMP) signal transduction pathway. Disruption of...

The Cryptococcus neoformans Catalase Gene Family and Its Role in Antioxidant Defense

Giles, Steven S., Stajich, Jason E., Nichols, Connie, Gerrald, Quincy D., Alspaugh, J. Andrew, Dietrich, Fred, ...

In the present study, we sought to elucidate the contribution of the Cryptococcus neoformans catalase gene family to antioxidant defense. We employed bioinformatics techniques to identify four...

Role of Protein O-Mannosyltransferase Pmt4 in the Morphogenesis and Virulence of Cryptococcus neoformans▿

Olson, Gillian M., Fox, Deborah S., Wang, Ping, Alspaugh, J. Andrew, Buchanan, Kent L.

Protein O mannosylation is initiated in the endoplasmic reticulum by protein O-mannosyltransferases (Pmt proteins) and plays an important role in the secretion, localization, and function of many...

The Cryptococcus neoformans Rho-GDP Dissociation Inhibitor Mediates Intracellular Survival and Virulence▿

Price, Michael S., Nichols, Connie B., Alspaugh, J. Andrew

Rho-GDP dissociation inhibitors (Rho-GDI) are repressors of Rho-type monomeric GTPases that control fundamental cellular processes, such as cytoskeletal arrangement, vesicle trafficking, and...

Differential Effects of Inhibiting Chitin and 1,3-β-d-Glucan Synthesis in Ras and Calcineurin Mutants of Aspergillus fumigatus▿

Fortwendel, Jarrod R., Juvvadi, Praveen Rao, Pinchai, Nadthanan, Perfect, B. Zachary, Alspaugh, J. Andrew, Perfect, John R., ...

Aspergillus fumigatus must be able to properly form hyphae and maintain cell wall integrity in order to establish invasive disease. Ras proteins and calcineurin each have been implicated as having...

Subcellular Localization Directs Signaling Specificity of the Cryptococcus neoformans Ras1 Protein▿

Nichols, Connie B., Ferreyra, Jessica, Ballou, Elizabeth R., Alspaugh, J. Andrew

In the human fungal pathogen Cryptococcus neoformans, Ras signaling mediates sexual differentiation, morphogenesis, and pathogenesis. By studying Ras prenylation and palmitoylation in this organism,...