J. B. Dame

Gene sequence tags from Plasmodium falciparum genomic DNA fragments prepared by the "genease" activity of mung bean nuclease.

Reddy, G R, Chakrabarti, D, Schuster, S M, Ferl, R J, Almira, E C, Dame, J B

A genes-first approach to genome sequencing is described which efficiently generates gene sequence tags from genomic DNA. Mung bean nuclease (EC 3.1.30.1) cleaves the genomic DNA of many organisms...

Mechanism of pyrimethamine resistance in recent isolates of Plasmodium falciparum.

McCutchan, T F, Welsh, J A, Dame, J B, Quakyi, I A, Graves, P M, Drake, J C, ...

Clones of Plasmodium falciparum prepared from recent isolates of infected blood were studied to determine the molecular mechanism of naturally occurring pyrimethamine resistance. Total DNA, as well...

Lipid and lipopolysaccharide composition of Escherichia coli surface-altered mutants selected for resistance to levallorphan, tetracaine, and polymyxin.

Dame, J B, Shapiro, B M

Certain mutants of Escherichia coli with an altered permeability barrier have an essentially normal lipopolysaccharide, fatty acid, and phospholipid content, with a slight increase in the membrane...

Monitoring Babesia bovis infections in cattle by using PCR-based tests.

Calder, J A, Reddy, G R, Chieves, L, Courtney, C H, Littell, R, Livengood, J R, ...

The sensitivity and specificity of PCR tests based on the small-subunit rRNA gene sequence of Babesia bovis were compared in a blind study of experimentally infected cattle with the corresponding...

Use of polymyxin B, levallorphan, and tetracaine to isolate novel envelope mutants of Escherichia coli.

Dame, J B, Shapiro, B M

Mutants of Escherichia coli were isolated by their resistance to the bacteriocidal effects of the membrane-active drugs polymyxin B, levallorphan, and tetracaine. The mutants were examined for...

rRNA-based method for sensitive detection of Babesia bigemina in bovine blood.

Reddy, G R, Dame, J B

Three synthetic oligonucleotide probes complementary to unique regions of Babesia bigemina small-subunit rRNA were developed for detecting the parasite in bovine blood. These probes specifically...

Two major sequence classes of ribosomal RNA genes in Plasmodium berghei.

Dame, J B, Sullivan, M, McCutchan, T F

Primary sequence differences have been found between two different ribosomal DNA (rDNA) units of the rodent malaria parasite, Plasmodium berghei, within the coding areas of both the small and large...

Sequence microheterogeneity of the three small subunit ribosomal RNA genes of Babesia bigemina: expression in erythrocyte culture.

Reddy, G R, Chakrabarti, D, Yowell, C A, Dame, J B

Three distinct ribosomal RNA (rRNA) transcription units (rDNA units), designated A, B and C, were identified in the intraerythrocytic protozoan parasite, Babesia bigemina. These rDNA units were...

The genome of Plasmodium cynomolgi is partitioned into separable domains which appear to differ in sequence stability.

McCutchan, T F, Dame, J B, Gwadz, R W, Vernick, K D

The genome of Plasmodium cynomolgi is partitioned into at least 7 distinct genetic domains. Each domain is apparently uniform in DNA density and is separable from the others by CsCl density...

Host Movement and the Genetic Structure of Populations of Parasitic Nematodes

Blouin, M. S., Yowell, C. A., Courtney, C. H., Dame, J. B.

Mitochondrial DNA (mtDNA) sequence data were used to compare the population genetic structures of five species of parasitic nematodes from three different hosts: Ostertagia ostertagi and Haemonchus...

Gene sequence tags from Plasmodium falciparum genomic DNA fragments prepared by the "genease" activity of mung bean nuclease.

Reddy, G R, Chakrabarti, D, Schuster, S M, Ferl, R J, Almira, E C, Dame, J B

A genes-first approach to genome sequencing is described which efficiently generates gene sequence tags from genomic DNA. Mung bean nuclease (EC 3.1.30.1) cleaves the genomic DNA of many organisms...

Mechanism of pyrimethamine resistance in recent isolates of Plasmodium falciparum.

McCutchan, T F, Welsh, J A, Dame, J B, Quakyi, I A, Graves, P M, Drake, J C, ...

Clones of Plasmodium falciparum prepared from recent isolates of infected blood were studied to determine the molecular mechanism of naturally occurring pyrimethamine resistance. Total DNA, as well...

Lipid and lipopolysaccharide composition of Escherichia coli surface-altered mutants selected for resistance to levallorphan, tetracaine, and polymyxin.

Dame, J B, Shapiro, B M

Certain mutants of Escherichia coli with an altered permeability barrier have an essentially normal lipopolysaccharide, fatty acid, and phospholipid content, with a slight increase in the membrane...

Monitoring Babesia bovis infections in cattle by using PCR-based tests.

Calder, J A, Reddy, G R, Chieves, L, Courtney, C H, Littell, R, Livengood, J R, ...

The sensitivity and specificity of PCR tests based on the small-subunit rRNA gene sequence of Babesia bovis were compared in a blind study of experimentally infected cattle with the corresponding...

Use of polymyxin B, levallorphan, and tetracaine to isolate novel envelope mutants of Escherichia coli.

Dame, J B, Shapiro, B M

Mutants of Escherichia coli were isolated by their resistance to the bacteriocidal effects of the membrane-active drugs polymyxin B, levallorphan, and tetracaine. The mutants were examined for...

rRNA-based method for sensitive detection of Babesia bigemina in bovine blood.

Reddy, G R, Dame, J B

Three synthetic oligonucleotide probes complementary to unique regions of Babesia bigemina small-subunit rRNA were developed for detecting the parasite in bovine blood. These probes specifically...

Two major sequence classes of ribosomal RNA genes in Plasmodium berghei.

Dame, J B, Sullivan, M, McCutchan, T F

Primary sequence differences have been found between two different ribosomal DNA (rDNA) units of the rodent malaria parasite, Plasmodium berghei, within the coding areas of both the small and large...

Sequence microheterogeneity of the three small subunit ribosomal RNA genes of Babesia bigemina: expression in erythrocyte culture.

Reddy, G R, Chakrabarti, D, Yowell, C A, Dame, J B

Three distinct ribosomal RNA (rRNA) transcription units (rDNA units), designated A, B and C, were identified in the intraerythrocytic protozoan parasite, Babesia bigemina. These rDNA units were...

The genome of Plasmodium cynomolgi is partitioned into separable domains which appear to differ in sequence stability.

McCutchan, T F, Dame, J B, Gwadz, R W, Vernick, K D

The genome of Plasmodium cynomolgi is partitioned into at least 7 distinct genetic domains. Each domain is apparently uniform in DNA density and is separable from the others by CsCl density...

Host Movement and the Genetic Structure of Populations of Parasitic Nematodes

Blouin, M. S., Yowell, C. A., Courtney, C. H., Dame, J. B.

Mitochondrial DNA (mtDNA) sequence data were used to compare the population genetic structures of five species of parasitic nematodes from three different hosts: Ostertagia ostertagi and Haemonchus...

Active site specificity of plasmepsin II.

Westling, J., Cipullo, P., Hung, S. H., Saft, H., Dame, J. B., Dunn, B. M.

Members of the aspartic proteinase family of enzymes have very similar three-dimensional structures and catalytic mechanisms. Each, however, has unique substrate specificity. These distinctions arise...