J. B. Le Pecq

The noncovalent complex between DNA and the bifunctional intercalator ditercalinium is a substrate for the UvrABC endonuclease of Escherichia coli.

Lambert, B, Jones, B K, Roques, B P, Le Pecq, J B, Yeung, A T

We have demonstrated that the noncovalent complex formed between DNA and an antitumor bifunctional intercalator, ditercalinium, is recognized in vitro as bulky covalent DNA lesions by the purified...

Geometry of the antitumor drug ditercalinium bisintercalated into d(CpGpCpG)2 by 1H NMR.

Delbarre, A, Delepierre, M, Garbay, C, Igolen, J, Le Pecq, J B, Roques, B P

Rigid 7H-pyrido[4,3-c]carbazole dimers, such as Ditercalinium, are DNA bisintercalators that display high DNA affinity and strong antitumor properties. This activity appears crucially dependent on...

1H NMR study of an ethidium dimer poly(dA-dT) complex: evidence of a transition between bis and monointercalation.

Delbarre, A, Gourevitch, M I, Gaugain, B, Le Pecq, J B, Roques, B P

Comparative 1H NMR and optical studies of the interaction between poly(dA-dT), ethidium bromide (Et) and ethidium dimer (Et2) in 0.7 M NaCl are reported as a function of the temperature. Denaturation...

Induction of an abortive and futile DNA repair process in E. coli by the antitumor DNA bifunctional intercalator, ditercalinium: role in polA in death induction.

Lambert, B, Roques, B P, Le Pecq, J B

Ditercalinium, an antitumor bifunctional intercalator which forms a high affinity reversible complex with DNA, was found to be specifically cytotoxic for polA and lig7 E. coli strains. In the polA...

Effect of B-Z transition and nucleic acid structure on the conformational dynamics of bound ethidium dimer measured by hydrogen deuterium exchange kinetics.

Markovits, J, Ramstein, J, Roques, B P, Le Pecq, J B

Ethidium dimer is shown to bind by intercalation, almost equally well, to the B and Z form of poly[(dG-m5dC)].poly[(dG-m5dC)], whereas the ethidium monomer shows a strong preference for the B form....

A reexamination of the problem of resonance energy transfer between DNA intercalated chromophores using bisintercalating compounds.

Le Bret, M, Le Pecq, J B, Barbet, J, Roques, B P

The rate of energy transfer between DNA intercalated ethidium cations calculated by Paoletti and Le Pecq1 using the Forster theory differs from the measured one by a factor of twenty two, if the...

DNA polyintercalating drugs: DNA binding of diacridine derivatives.

Le Pecq, J B, Le Bret, M, Barbet, J, Roques, B

As a first step in the synthesis and the study of DNA polyintercalating drugs, dimers of acridines have been prepared. Their DNA binding properties have been studied. It has been determined that when...

The noncovalent complex between DNA and the bifunctional intercalator ditercalinium is a substrate for the UvrABC endonuclease of Escherichia coli.

Lambert, B, Jones, B K, Roques, B P, Le Pecq, J B, Yeung, A T

We have demonstrated that the noncovalent complex formed between DNA and an antitumor bifunctional intercalator, ditercalinium, is recognized in vitro as bulky covalent DNA lesions by the purified...

Geometry of the antitumor drug ditercalinium bisintercalated into d(CpGpCpG)2 by 1H NMR.

Delbarre, A, Delepierre, M, Garbay, C, Igolen, J, Le Pecq, J B, Roques, B P

Rigid 7H-pyrido[4,3-c]carbazole dimers, such as Ditercalinium, are DNA bisintercalators that display high DNA affinity and strong antitumor properties. This activity appears crucially dependent on...

1H NMR study of an ethidium dimer poly(dA-dT) complex: evidence of a transition between bis and monointercalation.

Delbarre, A, Gourevitch, M I, Gaugain, B, Le Pecq, J B, Roques, B P

Comparative 1H NMR and optical studies of the interaction between poly(dA-dT), ethidium bromide (Et) and ethidium dimer (Et2) in 0.7 M NaCl are reported as a function of the temperature. Denaturation...

Induction of an abortive and futile DNA repair process in E. coli by the antitumor DNA bifunctional intercalator, ditercalinium: role in polA in death induction.

Lambert, B, Roques, B P, Le Pecq, J B

Ditercalinium, an antitumor bifunctional intercalator which forms a high affinity reversible complex with DNA, was found to be specifically cytotoxic for polA and lig7 E. coli strains. In the polA...

Effect of B-Z transition and nucleic acid structure on the conformational dynamics of bound ethidium dimer measured by hydrogen deuterium exchange kinetics.

Markovits, J, Ramstein, J, Roques, B P, Le Pecq, J B

Ethidium dimer is shown to bind by intercalation, almost equally well, to the B and Z form of poly[(dG-m5dC)].poly[(dG-m5dC)], whereas the ethidium monomer shows a strong preference for the B form....

A reexamination of the problem of resonance energy transfer between DNA intercalated chromophores using bisintercalating compounds.

Le Bret, M, Le Pecq, J B, Barbet, J, Roques, B P

The rate of energy transfer between DNA intercalated ethidium cations calculated by Paoletti and Le Pecq1 using the Forster theory differs from the measured one by a factor of twenty two, if the...

DNA polyintercalating drugs: DNA binding of diacridine derivatives.

Le Pecq, J B, Le Bret, M, Barbet, J, Roques, B

As a first step in the synthesis and the study of DNA polyintercalating drugs, dimers of acridines have been prepared. Their DNA binding properties have been studied. It has been determined that when...