Jong, J.C. De, Wermenbol, A.G., Verweij-Uijterwaal, M.W., Slaterus, K.W., Wertheim-van Dillen, P.M.E., Doornum, G.J.J. Van, ...
Swenson, P D, Lowens, M S, Celum, C L, Hierholzer, J C
Adenoviruses (Ads) are an important cause of respiratory illness, conjunctivitis, and gastroenteritis, but they are seldom recognized as a potential cause of sexually transmitted disease. We...
Anderson, L J, Bingham, P, Hierholzer, J C
We identified several types of neutralization effected by F and G protein monoclonal antibodies (MAbs) reacted individually or as mixtures against respiratory syncytial virus (RSV). Neutralizing...
Kew, O M, Nottay, B K, Hatch, M H, Hierholzer, J C, Obijeski, J F
Enterovirus 70 isolates obtained in Asia and the Americas between 1980 and 1981 from cases of acute hemorrhagic conjunctivitis were found to be very closely related by RNase T1 oligonucleotide...
Sensitivity of NCI-H292 human lung mucoepidermoid cells for respiratory and other human viruses.
Hierholzer, J C, Castells, E, Banks, G G, Bryan, J A, McEwen, C T
NCI-H292 mucoepidermoid carcinoma cells from human lungs were shown in an earlier report to be a fully adequate substitute for primary rhesus monkey kidney (MK) cells for the isolation and...
Hierholzer, J C, Halonen, P E, Dahlen, P O, Bingham, P G, McDonough, M M
In addition to tests for the group-specific hexon antigen of adenoviruses, adenoviruses can be detected in clinical specimens by hybridization assays utilizing the widely shared base sequences of the...
Hierholzer, J C, Bingham, P G, Coombs, R A, Johansson, K H, Anderson, L J, Halonen, P E
An all-monoclonal antibody, time-resolved fluoroimmunoassay was compared with several enzyme immunoassays for the detection of respiratory syncytial virus and parainfluenza virus type 1, 2, and 3...
Molecular epidemiology and restriction site mapping of adenovirus type 3 genome types.
Adrian, T, Best, B, Hierholzer, J C, Wigand, R
One hundred thirty-eight strains of adenovirus type 3 isolated from patients in the United States, West Germany, and other regions between 1961 and 1984 were analyzed with six restriction...
Adenovirus type 8 strains were collected over a 19-year period from eye specimens from patients with keratoconjunctivitis. These strains were divided by restriction enzyme analysis with the...
Anderson, L J, Hierholzer, J C, Stone, Y O, Tsou, C, Fernie, B F
To characterize the interrelationship of monoclonal antibodies (MAbs) against respiratory syncytial virus (RSV) and their respective epitopes, we developed a competitive binding assay based on the...
Hierholzer, J C, Johansson, K H, Anderson, L J, Tsou, C J, Halonen, P E
A sensitive time-resolved fluoroimmunoassay (TR-FIA), adapted from TR-FIA procedures already described, was developed with monoclonal antibodies and compared with several enzyme immunoassays (EIAs)...
Freeze-drying of respiratory syncytial viruses for transportation and storage.
Tannock, G A, Hierholzer, J C, Bryce, D A, Chee, C F, Paul, J A
The stabilities of eight strains of respiratory syncytial virus were compared after the strains were freeze-dried in the presence and absence of the stabilizer SPGA, which contains 218 mM sucrose,...
Anderson, L J, Godfrey, E, McIntosh, K, Hierholzer, J C
To develop and evaluate an enzyme-linked immunosorbent assay (ELISA) for detecting adenovirus antigens in which a group-specific monoclonal antibody to the adenovirus hexon is used, we studied two...
Hierholzer, J C, Bingham, P G, Coombs, R A, Stone, Y O, Hatch, M H
We describe here a microneutralization procedure for conveniently testing large numbers of specimens for antibodies to enterovirus 70. The test utilized human rhabdomyosarcoma cells and was read by...
Anderson, L J, Coombs, R A, Tsou, C, Hierholzer, J C
To characterize the specificity of monoclonal antibodies against respiratory syncytial virus, we developed a technique which combined the biotin-avidin system, immunoprecipitation, and transblot...
Brooks, J B, Nunez-Montiel, O L, Basta, M T, Hierholzer, J C
Thirty-five patients with various diarrheal syndromes and 22 controls were studied. All stool samples were carefully cultured for Clostridium difficile, using selective isolation media. Cytotoxin...
Microneutralization test for respiratory syncytial virus based on an enzyme immunoassay.
Anderson, L J, Hierholzer, J C, Bingham, P G, Stone, Y O
Virus infectivity and antibody neutralization titers for respiratory syncytial virus were determined in cell cultures in microtiter plates. After an appropriate incubation period, the cells were...
New human adenovirus associated with respiratory illness: candidate adenovirus type 39.
Hierholzer, J C, Kemp, M C, Gary, G W, Spencer, H C
A new human adenovirus was isolated from a stool specimen from a 2-year-old El Salvadorian male hospitalized with severe respiratory illness. The virus, strain D335, is a typical adenovirus by...
Antigenically intermediate human adenovirus strain associated with conjunctivitis.
Hierholzer, J C, Rodriguez, F H
Two strains of an antigenically intermediate human adenovirus were isolated from eye specimens from cases of conjunctivitis. The viruses were doubly neutralized by antisera to adenoviruses 15 and 29...
Stalder, H, Hierholzer, J C, Oxman, M N
An antigenically distinct adenovirus is described which was isolated in March 1973 from the lungs and kidney of a 61-year-old woman who died of diffuse interstitial adenovirus pneumonia 55 days after...
Crandell, R A, Hierholzer, J C, Krebs, J W, Drysdale, S S
Two different viruses were isolated from bovine embryonic cell cultures after two subcultures from the primary cells. One virus was identified as parainfluenza type 2 simian virus 5 (SV-5), and the...
Reference equine antisera to 33 human adenovirus types: homologous and heterologous titers.
Hierholzer, J C, Gamble, W C, Dowdle, W R
Equine antisera to human adenovirus types 1 to 33 were prepared and evaluated by hemagglutination-inhibition and serum neutralization tests. Detailed data on the potency and purity of the immunizing...
Vero microcultures for adenovirus neutralization tests.
A microculture neutralization test is described for measuring specific antibody levels to the 35 human adenovirus serotypes in Vero cells. The test is read at 5 days by macroscopic observation after...
Hierholzer, J C, Atuk, N O, Gwaltney, J M
An antigenically distinct adenovirus is described which was isolated in March 1972 from the urine of a 17-year-old Caucasian male who was experiencing fever after receiving a kidney transplant from a...
Fluorescein-isothiocyanate (FITC) conjugates were prepared by improved methods from standard reference antisera to influenza A and B, mumps, parainfluenza 1, 2, 3, and 4, herpesvirus, respiratory...
Adenoviruses in the immunocompromised host.
Adenoviruses are among the many pathogens and opportunistic agents that cause serious infection in the congenitally immunocompromised, in patients undergoing immunosuppressive treatment for organ and...
Dowdle, W. R., Lambriex, M., Hierholzer, J. C.
A simple procedure for the production of large volumes of purified adenovirus group-specific complement-fixing (CF) (hexon) antigen by selective adsorption to and elution from CaHPO4 is described....
Palmer, E. L., Martin, M. L., Hierholzer, J. C., Ziegler, D. W.
The anionic detergent sodium lauryl sulfate (SLS), in a final concentration of 0.1% and greater, reacted with whole serum in agar diffusion and immunoelectrophoresis to form artifactual precipitin...
New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice.
Hierholzer, J C, Broderson, J R, Murphy, F A
A new strain of mouse hepatitis virus (MHV) was isolated from pooled gut suspensions from an epizootic of lethal enteritis in newborn mice. Negative-contrast electron microscopy showed an abundance...
An unusual variant of adenovirus (AV) 11 was isolated from throat and rectal swabs from six persons with upper respiratory illness in a Spanish military camp in March 1969. The same strain was...
Swenson, P D, Lowens, M S, Celum, C L, Hierholzer, J C
Adenoviruses (Ads) are an important cause of respiratory illness, conjunctivitis, and gastroenteritis, but they are seldom recognized as a potential cause of sexually transmitted disease. We...
Anderson, L J, Bingham, P, Hierholzer, J C
We identified several types of neutralization effected by F and G protein monoclonal antibodies (MAbs) reacted individually or as mixtures against respiratory syncytial virus (RSV). Neutralizing...
Kew, O M, Nottay, B K, Hatch, M H, Hierholzer, J C, Obijeski, J F
Enterovirus 70 isolates obtained in Asia and the Americas between 1980 and 1981 from cases of acute hemorrhagic conjunctivitis were found to be very closely related by RNase T1 oligonucleotide...
Sensitivity of NCI-H292 human lung mucoepidermoid cells for respiratory and other human viruses.
Hierholzer, J C, Castells, E, Banks, G G, Bryan, J A, McEwen, C T
NCI-H292 mucoepidermoid carcinoma cells from human lungs were shown in an earlier report to be a fully adequate substitute for primary rhesus monkey kidney (MK) cells for the isolation and...
Hierholzer, J C, Halonen, P E, Dahlen, P O, Bingham, P G, McDonough, M M
In addition to tests for the group-specific hexon antigen of adenoviruses, adenoviruses can be detected in clinical specimens by hybridization assays utilizing the widely shared base sequences of the...
Hierholzer, J C, Bingham, P G, Coombs, R A, Johansson, K H, Anderson, L J, Halonen, P E
An all-monoclonal antibody, time-resolved fluoroimmunoassay was compared with several enzyme immunoassays for the detection of respiratory syncytial virus and parainfluenza virus type 1, 2, and 3...
Molecular epidemiology and restriction site mapping of adenovirus type 3 genome types.
Adrian, T, Best, B, Hierholzer, J C, Wigand, R
One hundred thirty-eight strains of adenovirus type 3 isolated from patients in the United States, West Germany, and other regions between 1961 and 1984 were analyzed with six restriction...
Adenovirus type 8 strains were collected over a 19-year period from eye specimens from patients with keratoconjunctivitis. These strains were divided by restriction enzyme analysis with the...
Anderson, L J, Hierholzer, J C, Stone, Y O, Tsou, C, Fernie, B F
To characterize the interrelationship of monoclonal antibodies (MAbs) against respiratory syncytial virus (RSV) and their respective epitopes, we developed a competitive binding assay based on the...
Hierholzer, J C, Johansson, K H, Anderson, L J, Tsou, C J, Halonen, P E
A sensitive time-resolved fluoroimmunoassay (TR-FIA), adapted from TR-FIA procedures already described, was developed with monoclonal antibodies and compared with several enzyme immunoassays (EIAs)...
Freeze-drying of respiratory syncytial viruses for transportation and storage.
Tannock, G A, Hierholzer, J C, Bryce, D A, Chee, C F, Paul, J A
The stabilities of eight strains of respiratory syncytial virus were compared after the strains were freeze-dried in the presence and absence of the stabilizer SPGA, which contains 218 mM sucrose,...
Anderson, L J, Godfrey, E, McIntosh, K, Hierholzer, J C
To develop and evaluate an enzyme-linked immunosorbent assay (ELISA) for detecting adenovirus antigens in which a group-specific monoclonal antibody to the adenovirus hexon is used, we studied two...
Hierholzer, J C, Bingham, P G, Coombs, R A, Stone, Y O, Hatch, M H
We describe here a microneutralization procedure for conveniently testing large numbers of specimens for antibodies to enterovirus 70. The test utilized human rhabdomyosarcoma cells and was read by...
Anderson, L J, Coombs, R A, Tsou, C, Hierholzer, J C
To characterize the specificity of monoclonal antibodies against respiratory syncytial virus, we developed a technique which combined the biotin-avidin system, immunoprecipitation, and transblot...
Brooks, J B, Nunez-Montiel, O L, Basta, M T, Hierholzer, J C
Thirty-five patients with various diarrheal syndromes and 22 controls were studied. All stool samples were carefully cultured for Clostridium difficile, using selective isolation media. Cytotoxin...
Microneutralization test for respiratory syncytial virus based on an enzyme immunoassay.
Anderson, L J, Hierholzer, J C, Bingham, P G, Stone, Y O
Virus infectivity and antibody neutralization titers for respiratory syncytial virus were determined in cell cultures in microtiter plates. After an appropriate incubation period, the cells were...
New human adenovirus associated with respiratory illness: candidate adenovirus type 39.
Hierholzer, J C, Kemp, M C, Gary, G W, Spencer, H C
A new human adenovirus was isolated from a stool specimen from a 2-year-old El Salvadorian male hospitalized with severe respiratory illness. The virus, strain D335, is a typical adenovirus by...
Antigenically intermediate human adenovirus strain associated with conjunctivitis.
Hierholzer, J C, Rodriguez, F H
Two strains of an antigenically intermediate human adenovirus were isolated from eye specimens from cases of conjunctivitis. The viruses were doubly neutralized by antisera to adenoviruses 15 and 29...
Stalder, H, Hierholzer, J C, Oxman, M N
An antigenically distinct adenovirus is described which was isolated in March 1973 from the lungs and kidney of a 61-year-old woman who died of diffuse interstitial adenovirus pneumonia 55 days after...
Crandell, R A, Hierholzer, J C, Krebs, J W, Drysdale, S S
Two different viruses were isolated from bovine embryonic cell cultures after two subcultures from the primary cells. One virus was identified as parainfluenza type 2 simian virus 5 (SV-5), and the...
Reference equine antisera to 33 human adenovirus types: homologous and heterologous titers.
Hierholzer, J C, Gamble, W C, Dowdle, W R
Equine antisera to human adenovirus types 1 to 33 were prepared and evaluated by hemagglutination-inhibition and serum neutralization tests. Detailed data on the potency and purity of the immunizing...
Vero microcultures for adenovirus neutralization tests.
A microculture neutralization test is described for measuring specific antibody levels to the 35 human adenovirus serotypes in Vero cells. The test is read at 5 days by macroscopic observation after...
Hierholzer, J C, Atuk, N O, Gwaltney, J M
An antigenically distinct adenovirus is described which was isolated in March 1972 from the urine of a 17-year-old Caucasian male who was experiencing fever after receiving a kidney transplant from a...
Fluorescein-isothiocyanate (FITC) conjugates were prepared by improved methods from standard reference antisera to influenza A and B, mumps, parainfluenza 1, 2, 3, and 4, herpesvirus, respiratory...
Adenoviruses in the immunocompromised host.
Adenoviruses are among the many pathogens and opportunistic agents that cause serious infection in the congenitally immunocompromised, in patients undergoing immunosuppressive treatment for organ and...
Dowdle, W. R., Lambriex, M., Hierholzer, J. C.
A simple procedure for the production of large volumes of purified adenovirus group-specific complement-fixing (CF) (hexon) antigen by selective adsorption to and elution from CaHPO4 is described....
Palmer, E. L., Martin, M. L., Hierholzer, J. C., Ziegler, D. W.
The anionic detergent sodium lauryl sulfate (SLS), in a final concentration of 0.1% and greater, reacted with whole serum in agar diffusion and immunoelectrophoresis to form artifactual precipitin...
New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice.
Hierholzer, J C, Broderson, J R, Murphy, F A
A new strain of mouse hepatitis virus (MHV) was isolated from pooled gut suspensions from an epizootic of lethal enteritis in newborn mice. Negative-contrast electron microscopy showed an abundance...
An unusual variant of adenovirus (AV) 11 was isolated from throat and rectal swabs from six persons with upper respiratory illness in a Spanish military camp in March 1969. The same strain was...