Multi-site development of a FIRA large league robot football system (2008)
J. C. Wolf, J. D. Oliver, P. Robinson, C. Diot
The difficulties associated with developing a competitive FIRA team using undergraduate students, spread across multiple sites are discussed. System modularisation and common interface standards are...
Cohen, A. L. V., Oliver, J. D., DePaola, A., Feil, E. J., Boyd, E. F.
Vibrio vulnificus is a ubiquitous inhabitant of the marine coastal environment, and an important pathogen of humans. We characterized a globally distributed sample of environmental isolates from a...
Abell, B. M., Jung, M., Oliver, J. D., Knight, B. C., Tyedmers, J., Zimmermann, R., ...
Tail-anchored proteins are a distinct class of membrane proteins that are characterized by a C-terminal membrane insertion sequence and a capacity for post-translational integration. Although it is...
Oliver, J. D., Van Den Avyle, Michael J.
Species profiles are literature summaries of the life history, distribution, and environmental requirements of coastal fishes and invertebrates. Profiles are prepared to assist with environmental...
Yaw Induced in Bullet Flight by Passage through Foliage - Initial Study: 7.62mm Bullet. (1998)
It is found that as little as half a metre of foliage, lightly packed at a density typical of such vegetation, will induce vigorous and often irregular precessional motion in the 7.62 mm bullet, the...
Effects of engineered salt bridges on the stability of subtilisin BPN' (1990)
Erwin, C.R., Barnett, B.L., Oliver, J.D., Sullivan, J.F.
Variants designed using PROTEUS have been produced in an attempt to engineer stabilizing salt bridges into subtilisin BPN'. All the mutants constructed by site-directed mutagenesis were secreted by...
Lowder, M., Unge, A., Maraha, N., Jansson, J. K., Swiggett, J., Oliver, J. D.
The green fluorescent protein (GFP) gene, gfp, of the jellyfish Aequorea victoria is being used as a reporter system for gene expression and as a marker for tracking prokaryotes and eukaryotes. Cells...
Vibrio vulnificus is an estuarine bacterium responsible for 95% of all seafood-related deaths in the United States. The bacterium occurs naturally in molluscan shellfish, and ingestion of raw oysters...
Oliver, J D, Hite, F, McDougald, D, Andon, N L, Simpson, L M
Using plate counts, total cell counts, and direct viable counts, we examined the fate of cells of Vibrio vulnificus placed into natural estuarine waters during both winter and summer months. Cells...
Effect of low temperature on starvation-survival of the eel pathogen Vibrio vulnificus biotype 2.
Biosca, E G, Amaro, C, Marco-Noales, E, Oliver, J D
At present, no reports exist on the isolation of the eel pathogen Vibrio vulnificus biotype 2 from water samples. Nevertheless, it has recently been demonstrated that this biotype can use water as a...
Biosca, E G, Oliver, J D, Amaro, C
In this study, we have reevaluated the taxonomic position of biotype 2 of Vibrio vulnificus. For this purpose, we have biochemically and serologically characterized 83 biotype 2 strains from diseased...
Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification.
Coleman, S S, Melanson, D M, Biosca, E G, Oliver, J D
DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with...
Høi, L, Dalsgaard, A, Larsen, J L, Warner, J M, Oliver, J D
A total of 85 isolates of Vibrio vulnificus were characterized by ribotyping with a probe complementary to 16S and 23S rRNA of Escherichia coli and by randomly amplified polymorphic DNA-PCR...
Induction of cold-responsive proteins in Vibrio vulnificus.
We have studied the response of Vibrio vulnificus to temperature shifts (23 to 13 degrees C) within the organism's permissive growth range. Cold shift induced a diminution in protein synthesis....
Effects of temperature abuse on survival of Vibrio vulnificus in oysters.
Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days....
Formation of nonculturable Vibrio vulnificus cells and its relationship to the starvation state.
Oliver, J D, Nilsson, L, Kjelleberg, S
Entry into the viable but nonculturable state by the human bacterial pathogen Vibrio vulnificus in artificial seawater microcosms was studied. In contrast to the long-term culturability exhibited by...
Brauns, L A, Hudson, M C, Oliver, J D
Vibrio vulnificus is a human pathogen associated with consumption of raw oysters. During the colder months the organism apparently enters a viable but nonculturable state and thus cannot be cultured...
Use of colistin-polymyxin B-cellobiose agar for isolation of Vibrio vulnificus from the environment.
Oliver, J D, Guthrie, K, Preyer, J, Wright, A, Simpson, L M, Siebeling, R, ...
Colistin-polymyxin B-cellobiose agar was employed for the isolation of Vibrio vulnificus from shellfish. Isolates were examined phenotypically and with a gene probe and monoclonal antibody specific...
RpoS-Dependent Stress Response and Exoenzyme Production in Vibrio vulnificus
Hülsmann, A., Rosche, T. M., Hassan, H. M., Beam, D. M., Oliver, J. D.
Vibrio vulnificus is an estuarine bacterium capable of causing rapidly fatal infections through both ingestion and wound infection. Like other opportunistic pathogens, V. vulnificus must adapt to...
The nonculturable state of Vibrio vulnificus and, for comparison, that of Escherichia coli were studied in artificial-seawater microcosms at 5 degrees C. Total cell counts were monitored by acridine...
Plasmid carriage in Vibrio vulnificus and other lactose-fermenting marine vibrios.
A total of 42 clinical and environmental isolates of Vibrio vulnificus were examined for plasmid carriage. Of these, only five (12%) harbored plasmids, which were of various molecular weights. In...
Morris, J G, Wright, A C, Roberts, D M, Wood, P K, Simpson, L M, Oliver, J D
We screened 44 lactose-positive Vibrio strains isolated from the marine environment for homology with a 3.2-kilobase DNA fragment encoding the Vibrio vulnificus cytotoxin-hemolysin gene. All 29...
New selective and differential medium for Vibrio cholerae and Vibrio vulnificus.
Thiosulfate-citrate-bile salts-sucrose agar has been routinely used for the isolation of pathogenic vibrios, although its selectivity for Vibrio cholerae and Vibrio vulnificus is inadequate....
Resuscitation of Vibrio vulnificus from the viable but nonculturable state.
Nilsson, L, Oliver, J D, Kjelleberg, S
Stationary-phase-grown cells of the estuarine bacterium Vibrio vulnificus became nonculturable in nutrient-limited artificial seawater microcosms after 27 days at 5 degrees C. When the nonculturable...
Bioluminescence in a strain of the human pathogenic bacterium Vibrio vulnificus.
Oliver, J D, Roberts, D M, White, V K, Dry, M A, Simpson, L M
We report the existence of a bioluminescent strain of the human pathogen Vibrio vulnificus. The isolate was obtained from blood following a fatal wound infection and thus represents the first...
Oliver, J D, Warner, R A, Cleland, D R
Water, sediment, plankton, and animal samples from five coastal sites from North Carolina to Georgia were sampled for their lactose-fermenting vibrio populations. Over 20% of all vibrios tested were...
Lethal cold stress of Vibrio vulnificus in oysters.
Studies were conducted on the survival of Vibrio vulnificus, an estuarine human pathogen, in oyster homogenates held at 4 degrees C. Results indicated a rapid and dramatic decrease in viability not...
Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus.
Wright, A C, Simpson, L M, Oliver, J D, Morris, J G
Translucent, avirulent spontaneous phase variants of Vibrio vulnificus MO6-24 reverted back to the original opaque, encapsulated phenotype under both in vivo and in vitro conditions. Two translucent,...
Ability of Vibrio vulnificus to obtain iron from hemoglobin-haptoglobin complexes.
Zakaria-Meehan, Z, Massad, G, Simpson, L M, Travis, J C, Oliver, J D
It has been suggested that the normal serum protein, haptoglobin (Hp), serves a bacteriostatic role by binding free hemoglobin (Hm), thus making heme iron unavailable for bacterial growth. Previous...
Correlation between virulence and colony morphology in Vibrio vulnificus.
Simpson, L M, White, V K, Zane, S F, Oliver, J D
Of 38 isolates of Vibrio vulnificus examined, all avirulent strains produced only translucent colonies. All virulent strains, with the exception of biogroup 2 (eel pathogens), exhibited both opaque...
Role of heme compounds and haptoglobin in Vibrio vulnificus pathogenicity.
Helms, S D, Oliver, J D, Travis, J C
An induced peritonitis model was employed in mice to determine whether heme-containing molecules enhance the lethality of infections by Vibrio vulnificus. The lethality of intraperitoneal (ip)...
Siderophore production by Vibrio vulnificus.
Previous studies in our laboratory, as well as clinical evidence, have suggested that increased iron levels in the host may be important in infections caused by the halophilic pathogen Vibrio...
Survival of Helicobacter pylori in a Natural Freshwater Environment
Adams, B. L., Bates, T. C., Oliver, J. D.
The mode by which Helicobacter pylori, the causative agent of most gastric ulcers, is transmitted remains undetermined. Epidemiological evidence suggests these organisms are waterborne; however, H....
Role of iron in the pathogenesis of Vibrio vulnificus infections.
Wright, A C, Simpson, L M, Oliver, J D
Infections with Vibrio vulnificus resulting in septicemia and high mortality have been correlated with pre-existing liver disease and hemochromatosis. As these conditions are associated with impaired...
Edema and hemoconcentration in mice experimentally infected with Vibrio vulnificus.
Bowdre, J H, Poole, M D, Oliver, J D
Vibrio vulnificus (lactose-positive Vibrio), a recently recognized pathogenic marine species, produced extreme hemoconcentration and death within 3 to 6 h after subcutaneous or intraperitoneal...
Laboratory animals were challenged subcutaneously, intraperitoneally, and intravenously with the halophilic lactose-positive Vibrio. Intraperitoneal inocula of 10(8) organisms proved to be rapidly...
Mackay, A G, Oliver, J D, Rogers, M P
Tumour necrosis factor (TNF) has previously been shown to decrease lipoprotein lipase (LPL) activity and mRNA levels in 3T3-L1 cells and in adipose tissue from rats and guinea pigs when injected in...
Stimulation of lipoprotein lipase synthesis by refeeding, insulin and dexamethasone.
Lipoprotein lipase synthesis in adipose tissue was greater in rats fed ad libitum or refed than in fasted rats. Insulin alone and together with dexamethasone increased lipoprotein lipase synthesis in...
Resuscitation of Vibrio vulnificus from the Viable but Nonculturable State
Whitesides, M. D., Oliver, J. D.
Like many other gram-negative bacteria, the human pathogen Vibrio vulnificus is induced into a viable but nonculturable (VBNC) state by incubation at low temperatures. The ability of any bacterium to...
Lowder, M., Unge, A., Maraha, N., Jansson, J. K., Swiggett, J., Oliver, J. D.
The green fluorescent protein (GFP) gene, gfp, of the jellyfish Aequorea victoria is being used as a reporter system for gene expression and as a marker for tracking prokaryotes and eukaryotes. Cells...
Vibrio vulnificus is an estuarine bacterium responsible for 95% of all seafood-related deaths in the United States. The bacterium occurs naturally in molluscan shellfish, and ingestion of raw oysters...
Oliver, J D, Hite, F, McDougald, D, Andon, N L, Simpson, L M
Using plate counts, total cell counts, and direct viable counts, we examined the fate of cells of Vibrio vulnificus placed into natural estuarine waters during both winter and summer months. Cells...
Effect of low temperature on starvation-survival of the eel pathogen Vibrio vulnificus biotype 2.
Biosca, E G, Amaro, C, Marco-Noales, E, Oliver, J D
At present, no reports exist on the isolation of the eel pathogen Vibrio vulnificus biotype 2 from water samples. Nevertheless, it has recently been demonstrated that this biotype can use water as a...
Biosca, E G, Oliver, J D, Amaro, C
In this study, we have reevaluated the taxonomic position of biotype 2 of Vibrio vulnificus. For this purpose, we have biochemically and serologically characterized 83 biotype 2 strains from diseased...
Detection of Vibrio vulnificus biotypes 1 and 2 in eels and oysters by PCR amplification.
Coleman, S S, Melanson, D M, Biosca, E G, Oliver, J D
DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with...
Høi, L, Dalsgaard, A, Larsen, J L, Warner, J M, Oliver, J D
A total of 85 isolates of Vibrio vulnificus were characterized by ribotyping with a probe complementary to 16S and 23S rRNA of Escherichia coli and by randomly amplified polymorphic DNA-PCR...
Induction of cold-responsive proteins in Vibrio vulnificus.
We have studied the response of Vibrio vulnificus to temperature shifts (23 to 13 degrees C) within the organism's permissive growth range. Cold shift induced a diminution in protein synthesis....
Effects of temperature abuse on survival of Vibrio vulnificus in oysters.
Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days....
Formation of nonculturable Vibrio vulnificus cells and its relationship to the starvation state.
Oliver, J D, Nilsson, L, Kjelleberg, S
Entry into the viable but nonculturable state by the human bacterial pathogen Vibrio vulnificus in artificial seawater microcosms was studied. In contrast to the long-term culturability exhibited by...
Brauns, L A, Hudson, M C, Oliver, J D
Vibrio vulnificus is a human pathogen associated with consumption of raw oysters. During the colder months the organism apparently enters a viable but nonculturable state and thus cannot be cultured...
Use of colistin-polymyxin B-cellobiose agar for isolation of Vibrio vulnificus from the environment.
Oliver, J D, Guthrie, K, Preyer, J, Wright, A, Simpson, L M, Siebeling, R, ...
Colistin-polymyxin B-cellobiose agar was employed for the isolation of Vibrio vulnificus from shellfish. Isolates were examined phenotypically and with a gene probe and monoclonal antibody specific...
RpoS-Dependent Stress Response and Exoenzyme Production in Vibrio vulnificus
Hülsmann, A., Rosche, T. M., Hassan, H. M., Beam, D. M., Oliver, J. D.
Vibrio vulnificus is an estuarine bacterium capable of causing rapidly fatal infections through both ingestion and wound infection. Like other opportunistic pathogens, V. vulnificus must adapt to...
The nonculturable state of Vibrio vulnificus and, for comparison, that of Escherichia coli were studied in artificial-seawater microcosms at 5 degrees C. Total cell counts were monitored by acridine...
Plasmid carriage in Vibrio vulnificus and other lactose-fermenting marine vibrios.
A total of 42 clinical and environmental isolates of Vibrio vulnificus were examined for plasmid carriage. Of these, only five (12%) harbored plasmids, which were of various molecular weights. In...
Morris, J G, Wright, A C, Roberts, D M, Wood, P K, Simpson, L M, Oliver, J D
We screened 44 lactose-positive Vibrio strains isolated from the marine environment for homology with a 3.2-kilobase DNA fragment encoding the Vibrio vulnificus cytotoxin-hemolysin gene. All 29...
New selective and differential medium for Vibrio cholerae and Vibrio vulnificus.
Thiosulfate-citrate-bile salts-sucrose agar has been routinely used for the isolation of pathogenic vibrios, although its selectivity for Vibrio cholerae and Vibrio vulnificus is inadequate....
Resuscitation of Vibrio vulnificus from the viable but nonculturable state.
Nilsson, L, Oliver, J D, Kjelleberg, S
Stationary-phase-grown cells of the estuarine bacterium Vibrio vulnificus became nonculturable in nutrient-limited artificial seawater microcosms after 27 days at 5 degrees C. When the nonculturable...
Bioluminescence in a strain of the human pathogenic bacterium Vibrio vulnificus.
Oliver, J D, Roberts, D M, White, V K, Dry, M A, Simpson, L M
We report the existence of a bioluminescent strain of the human pathogen Vibrio vulnificus. The isolate was obtained from blood following a fatal wound infection and thus represents the first...
Oliver, J D, Warner, R A, Cleland, D R
Water, sediment, plankton, and animal samples from five coastal sites from North Carolina to Georgia were sampled for their lactose-fermenting vibrio populations. Over 20% of all vibrios tested were...
Lethal cold stress of Vibrio vulnificus in oysters.
Studies were conducted on the survival of Vibrio vulnificus, an estuarine human pathogen, in oyster homogenates held at 4 degrees C. Results indicated a rapid and dramatic decrease in viability not...
Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus.
Wright, A C, Simpson, L M, Oliver, J D, Morris, J G
Translucent, avirulent spontaneous phase variants of Vibrio vulnificus MO6-24 reverted back to the original opaque, encapsulated phenotype under both in vivo and in vitro conditions. Two translucent,...
Ability of Vibrio vulnificus to obtain iron from hemoglobin-haptoglobin complexes.
Zakaria-Meehan, Z, Massad, G, Simpson, L M, Travis, J C, Oliver, J D
It has been suggested that the normal serum protein, haptoglobin (Hp), serves a bacteriostatic role by binding free hemoglobin (Hm), thus making heme iron unavailable for bacterial growth. Previous...
Correlation between virulence and colony morphology in Vibrio vulnificus.
Simpson, L M, White, V K, Zane, S F, Oliver, J D
Of 38 isolates of Vibrio vulnificus examined, all avirulent strains produced only translucent colonies. All virulent strains, with the exception of biogroup 2 (eel pathogens), exhibited both opaque...
Role of heme compounds and haptoglobin in Vibrio vulnificus pathogenicity.
Helms, S D, Oliver, J D, Travis, J C
An induced peritonitis model was employed in mice to determine whether heme-containing molecules enhance the lethality of infections by Vibrio vulnificus. The lethality of intraperitoneal (ip)...
Siderophore production by Vibrio vulnificus.
Previous studies in our laboratory, as well as clinical evidence, have suggested that increased iron levels in the host may be important in infections caused by the halophilic pathogen Vibrio...
Survival of Helicobacter pylori in a Natural Freshwater Environment
Adams, B. L., Bates, T. C., Oliver, J. D.
The mode by which Helicobacter pylori, the causative agent of most gastric ulcers, is transmitted remains undetermined. Epidemiological evidence suggests these organisms are waterborne; however, H....
Role of iron in the pathogenesis of Vibrio vulnificus infections.
Wright, A C, Simpson, L M, Oliver, J D
Infections with Vibrio vulnificus resulting in septicemia and high mortality have been correlated with pre-existing liver disease and hemochromatosis. As these conditions are associated with impaired...
Edema and hemoconcentration in mice experimentally infected with Vibrio vulnificus.
Bowdre, J H, Poole, M D, Oliver, J D
Vibrio vulnificus (lactose-positive Vibrio), a recently recognized pathogenic marine species, produced extreme hemoconcentration and death within 3 to 6 h after subcutaneous or intraperitoneal...
Laboratory animals were challenged subcutaneously, intraperitoneally, and intravenously with the halophilic lactose-positive Vibrio. Intraperitoneal inocula of 10(8) organisms proved to be rapidly...
Mackay, A G, Oliver, J D, Rogers, M P
Tumour necrosis factor (TNF) has previously been shown to decrease lipoprotein lipase (LPL) activity and mRNA levels in 3T3-L1 cells and in adipose tissue from rats and guinea pigs when injected in...
Stimulation of lipoprotein lipase synthesis by refeeding, insulin and dexamethasone.
Lipoprotein lipase synthesis in adipose tissue was greater in rats fed ad libitum or refed than in fasted rats. Insulin alone and together with dexamethasone increased lipoprotein lipase synthesis in...
Resuscitation of Vibrio vulnificus from the Viable but Nonculturable State
Whitesides, M. D., Oliver, J. D.
Like many other gram-negative bacteria, the human pathogen Vibrio vulnificus is induced into a viable but nonculturable (VBNC) state by incubation at low temperatures. The ability of any bacterium to...