J. D. Priddle

Publication List Details

Period

2003 - 2003

Number

21

Co-Authors

Changes in lymphokine receptor expression and fatty acid composition of phospholipids and triacylglycerols in rat adipocytes associated with lymph nodes following a transient immune challenge (2003)

Priddle, J.D., Mattacks, C.A., Sadler, D.A., MacQueen, H.A., Pond, C.M.

Single-photon counting fluorimetry was used to record the time course of the expression of interleukin-10 receptors labelled with fluorescent antibodies on the surface of adipocytes over 24 hours,...

A mass-spectrometric method for the estimation of the ratio of gamma-carboxyglutamic acid to glutamic acid at specific sites in proteins. Application to the N-terminal region of bovine prothrombin.

Rose, K, Priddle, J D, Offord, R E, Esnouf, M P

When a polypeptide containing gamma-carboxyglutamic acid is decarboxylated in 2H2O, residue of (gamma gamma-2H2)glutamic acid are formed. Subsequent proteolytic digestion produces peptides which...

The separation and sequencing of permethylated peptides by mass spectrometry directly coupled to gas-liquid chromatography.

Priddle, J D, Rose, K, Offord, R E

The use of g.l.c. coupled to mass spectrometry to separate and sequence permethylated acetyl- and trifluoroacetyl-peptides in a single operation is described. Both electron impact and chemical...

Studies on the subunit structure and amino acid sequence of triose phosphate isomerase from chicken breast muscle

Furth, Anna J., Milman, J. D., Priddle, J. D., Offord, R. E.

1. Triose phosphate isomerase was prepared by chromatography on DEAE-cellulose of an (NH4)2SO4 fraction of an extract of homogenized chicken breast muscle. The product is homogeneous on gel...

Active-site labelling of triose phosphate isomerase. The reaction of bromohydroxyacetone phosphate with a unique glutamic acid residue and the migration of the label to tyrosine

De La Mare, Susan, Coulson, A. F. W., Knowles, J. R., Priddle, J. D., Offord, R. E.

Triose phosphate isomerase from chicken muscle reacts stoicheiometrically with the active-site-directed irreversible inhibitor bromohydroxyacetone phosphate with concomitant loss of all catalytic...

A mass-spectrometric method for the estimation of the ratio of gamma-carboxyglutamic acid to glutamic acid at specific sites in proteins. Application to the N-terminal region of bovine prothrombin.

Rose, K, Priddle, J D, Offord, R E, Esnouf, M P

When a polypeptide containing gamma-carboxyglutamic acid is decarboxylated in 2H2O, residue of (gamma gamma-2H2)glutamic acid are formed. Subsequent proteolytic digestion produces peptides which...

The separation and sequencing of permethylated peptides by mass spectrometry directly coupled to gas-liquid chromatography.

Priddle, J D, Rose, K, Offord, R E

The use of g.l.c. coupled to mass spectrometry to separate and sequence permethylated acetyl- and trifluoroacetyl-peptides in a single operation is described. Both electron impact and chemical...

Studies on the subunit structure and amino acid sequence of triose phosphate isomerase from chicken breast muscle

Furth, Anna J., Milman, J. D., Priddle, J. D., Offord, R. E.

1. Triose phosphate isomerase was prepared by chromatography on DEAE-cellulose of an (NH4)2SO4 fraction of an extract of homogenized chicken breast muscle. The product is homogeneous on gel...

Active-site labelling of triose phosphate isomerase. The reaction of bromohydroxyacetone phosphate with a unique glutamic acid residue and the migration of the label to tyrosine

De La Mare, Susan, Coulson, A. F. W., Knowles, J. R., Priddle, J. D., Offord, R. E.

Triose phosphate isomerase from chicken muscle reacts stoicheiometrically with the active-site-directed irreversible inhibitor bromohydroxyacetone phosphate with concomitant loss of all catalytic...

Characterisation and quantification of mucosal cytokine that induces epithelial histocompatibility locus antigen-DR expression in inflammatory bowel disease.

Lowes, J R, Radwan, P, Priddle, J D, Jewell, D P

Epithelial histocompatibility locus antigen (HLA) class II expression was studied to evaluate its induction by mucosal mononuclear cells in inflammatory bowel disease and to characterise the...

Production of epithelial cell growth factors by lamina propria mononuclear cells.

Lowes, J R, Priddle, J D, Jewell, D P

The effects of lamina propria mononuclear cell culture supernatant on epithelial cell DNA synthesis were studied using cells isolated from patients with inflammatory bowel disease and normal...

Cellular hypersensitivity to a synthetic dodecapeptide derived from human adenovirus 12 which resembles a sequence of A-gliadin in patients with coeliac disease.

Mantzaris, G J, Karagiannis, J A, Priddle, J D, Jewell, D P

The human intestinal adenovirus serotype 12 (Ad12) may be implicated in the pathogenesis of coeliac disease by virtue of immunological cross reactivity between epitopes shared by its early region E1b...

Serum and tissue autoantibodies to colonic epithelium in ulcerative colitis.

Snook, J A, Lowes, J R, Wu, K C, Priddle, J D, Jewell, D P

Sera and colonic tissue-bound immunoglobulin extracts from patients with ulcerative colitis and disease controls were examined immunohistochemically and by killer cell cytotoxicity assay for the...

Comparison of 5-aminosalicylic acid and N-acetylaminosalicylic acid uptake by the isolated human colonic epithelial cell.

Ireland, A, Priddle, J D, Jewell, D P

Isolated human colonic epithelial cell suspensions were incubated with either 0.1 mM 5-aminosalicylic acid (5-ASA) or 0.1 mM acetylaminosalicylic acid (Ac-ASA) for up to two hours. Intra- and...

Effects of olsalazine in the jejunum of the rat.

Mohsen, A Q, Mulvey, D, Priddle, J D, Parsons, D S, Jewell, D P

Olsalazine (ADS) is the azo-linked dimer of 5-aminosalicylic acid (5-ASA). It is of value for the management of patients with ulcerative colitis but may be associated with increasing diarrhoea in a...

Cellular hypersensitivity to gluten derived peptides in coeliac disease.

Guan, R, Rawcliffe, P M, Priddle, J D, Jewell, D P

Wheat gluten derived antigens have been tested for their ability to inhibit the migration of leucocytes from healthy subjects and patients with coeliac disease. Three preparations of a water soluble...

Colonic bacterial proteases to IgA1 and sIgA in patients with ulcerative colitis.

Barr, G D, Hudson, M J, Priddle, J D, Jewell, D P

The colonic faecal and mucosal associated bacterial populations of five patients with ulcerative colitis and four control patients were studied in detail to assess their ability to produce...

Immunoglobulin production by isolated intestinal mononuclear cells from patients with ulcerative colitis and Crohn's disease.

Wu, K C, Mahida, Y R, Priddle, J D, Jewell, D P

We studied immunoglobulin production by isolated intestinal mononuclear cells from 25 patients with active inflammatory bowel disease (IBD) and 17 controls undergoing surgical resections for...

Effect of human intestinal macrophages on immunoglobulin production by human intestinal mononuclear cells isolated from patients with inflammatory bowel disease.

Wu, K C, Mahida, Y R, Priddle, J D, Jewell, D P

The effect of macrophages on spontaneous immunoglobulin production by isolated human intestinal mononuclear cells (MNC) is unknown. Depletion of macrophages by adherence to fibronectin or by panning...