Double-stranded ribonucleic acid in Agaricus bisporus.
Marino, R, Saksena, K N, Schuler, M, Mayfield, J E, Lemke, P A
Double-stranded ribonucleic acid present in virus-infected mushrooms of Agaricus bisporus has been resolved through polyacrylamide gel electrophoresis into six molecular-weight forms. Identification...
Brucella abortus catalase is a periplasmic protein lacking a standard signal sequence.
Sha, Z, Stabel, T J, Mayfield, J E
A periplasmic catalase has been purified and cloned from Brucella abortus. The functional enzyme is a tetramer with a subunit molecular weight of 55,000. All evidence indicates that a typical...
Cloning and cleavage site mapping of DNA from bovine herpesvirus 1 (Cooper strain).
Mayfield, J E, Good, P J, VanOort, H J, Campbell, A R, Reed, D E
Sequences representative of most of the bovine herpesvirus 1 (Cooper strain) DNa were cloned in the plasmid vector pBR322 at the HindIII site. EcoRI, HpaI, and BamHI restriction endonuclease sites...
Rock, D L, Beam, S L, Mayfield, J E
Here we have used the bovine herpesvirus type 1 (BHV-1) rabbit model together with in situ nucleic acid hybridization to identify and map viral RNA present in latently infected neurons. Radioactively...
Stabel, T J, Mayfield, J E, Tabatabai, L B, Wannemuehler, M J
Salmonella typhimurium chi 4064, an attenuated delta cya delta crp mutant of S. typhimurium SR-11, was shown to be avirulent in swine. S. typhimurium chi 4064 was used as a carrier for plasmid...
Stabel, T J, Mayfield, J E, Tabatabai, L B, Wannemuehler, M J
Salmonella typhimurium chi 4064, an attenuated delta cya delta crp mutant of S. typhimurium SR-11, was used as a carrier for the plasmid pBA31-R7. This plasmid codes for the expression of a...
Deletion of the BCSP31 gene of Brucella abortus by replacement.
Halling, S M, Detilleux, P G, Tatum, F M, Judge, B A, Mayfield, J E
The 31-kDa salt-extractable immunogenic protein, BCSP31, was deleted from several Brucella abortus strains by replacement with a marker gene encoding resistance to the antibiotics kanamycin and...
Stabel, T J, Mayfield, J E, Morfitt, D C, Wannemuehler, M J
Salmonella choleraesuis chi 3781, an attenuated [delta cya-12 delta(crp-cdt)19] mutant, was electroporated with the plasmid pBA31-R7, which codes for the expression of a 31-kDa protein from Brucella...
Cloning, expression, and occurrence of the Brucella Cu-Zn superoxide dismutase.
Bricker, B J, Tabatabai, L B, Judge, B A, Deyoe, B L, Mayfield, J E
Recently, the complete amino acid sequence of a protein expressed in Escherichia coli from cloned Brucella abortus DNA was reported. On the basis of amino acid homology, this protein was identified...
Carbohydrate and protein contents of grain dusts in relation to dust morphology.
Dashek, W V, Olenchock, S A, Mayfield, J E, Wirtz, G H, Wolz, D E, Young, C A
Grain dusts contain a variety of materials which are potentially hazardous to the health of workers in the grain industry. Because the characterization of grain dusts is incomplete, we are defining...
Replacement of Bovine Mitochondrial DNA by a Sequence Variant within One Generation
Koehler, C. M., Lindberg, G. L., Brown, D. R., Beitz, D. C., Freeman, A. E., Mayfield, J. E., ...
Inheritance of mitochondrial DNA (mtDNA) in Holstein cattle was characterized by pedigree analysis of nucleotide sequence variation. mtDNA was purified from leukocytes of 174 individuals representing...
Double-stranded ribonucleic acid in Agaricus bisporus.
Marino, R, Saksena, K N, Schuler, M, Mayfield, J E, Lemke, P A
Double-stranded ribonucleic acid present in virus-infected mushrooms of Agaricus bisporus has been resolved through polyacrylamide gel electrophoresis into six molecular-weight forms. Identification...
Brucella abortus catalase is a periplasmic protein lacking a standard signal sequence.
Sha, Z, Stabel, T J, Mayfield, J E
A periplasmic catalase has been purified and cloned from Brucella abortus. The functional enzyme is a tetramer with a subunit molecular weight of 55,000. All evidence indicates that a typical...
Cloning and cleavage site mapping of DNA from bovine herpesvirus 1 (Cooper strain).
Mayfield, J E, Good, P J, VanOort, H J, Campbell, A R, Reed, D E
Sequences representative of most of the bovine herpesvirus 1 (Cooper strain) DNa were cloned in the plasmid vector pBR322 at the HindIII site. EcoRI, HpaI, and BamHI restriction endonuclease sites...
Rock, D L, Beam, S L, Mayfield, J E
Here we have used the bovine herpesvirus type 1 (BHV-1) rabbit model together with in situ nucleic acid hybridization to identify and map viral RNA present in latently infected neurons. Radioactively...
Stabel, T J, Mayfield, J E, Tabatabai, L B, Wannemuehler, M J
Salmonella typhimurium chi 4064, an attenuated delta cya delta crp mutant of S. typhimurium SR-11, was shown to be avirulent in swine. S. typhimurium chi 4064 was used as a carrier for plasmid...
Stabel, T J, Mayfield, J E, Tabatabai, L B, Wannemuehler, M J
Salmonella typhimurium chi 4064, an attenuated delta cya delta crp mutant of S. typhimurium SR-11, was used as a carrier for the plasmid pBA31-R7. This plasmid codes for the expression of a...
Deletion of the BCSP31 gene of Brucella abortus by replacement.
Halling, S M, Detilleux, P G, Tatum, F M, Judge, B A, Mayfield, J E
The 31-kDa salt-extractable immunogenic protein, BCSP31, was deleted from several Brucella abortus strains by replacement with a marker gene encoding resistance to the antibiotics kanamycin and...
Stabel, T J, Mayfield, J E, Morfitt, D C, Wannemuehler, M J
Salmonella choleraesuis chi 3781, an attenuated [delta cya-12 delta(crp-cdt)19] mutant, was electroporated with the plasmid pBA31-R7, which codes for the expression of a 31-kDa protein from Brucella...
Cloning, expression, and occurrence of the Brucella Cu-Zn superoxide dismutase.
Bricker, B J, Tabatabai, L B, Judge, B A, Deyoe, B L, Mayfield, J E
Recently, the complete amino acid sequence of a protein expressed in Escherichia coli from cloned Brucella abortus DNA was reported. On the basis of amino acid homology, this protein was identified...
Replacement of Bovine Mitochondrial DNA by a Sequence Variant within One Generation
Koehler, C. M., Lindberg, G. L., Brown, D. R., Beitz, D. C., Freeman, A. E., Mayfield, J. E., ...
Inheritance of mitochondrial DNA (mtDNA) in Holstein cattle was characterized by pedigree analysis of nucleotide sequence variation. mtDNA was purified from leukocytes of 174 individuals representing...
Carbohydrate and protein contents of grain dusts in relation to dust morphology.
Dashek, W V, Olenchock, S A, Mayfield, J E, Wirtz, G H, Wolz, D E, Young, C A
Grain dusts contain a variety of materials which are potentially hazardous to the health of workers in the grain industry. Because the characterization of grain dusts is incomplete, we are defining...