J. E. Parrillo

Bioavailability of oral trimetrexate in patients with acquired immunodeficiency syndrome.

Rogers, P, Allegra, C J, Murphy, R F, Drake, J C, Masur, H, Poplack, D G, ...

The combination of the lipophilic antifolate trimetrexate and the rescue agent leucovorin has shown promise in the treatment of Pneumocystis carinii pneumonia in patients with acquired...

Potent antipneumocystis and antitoxoplasma activities of piritrexim, a lipid-soluble antifolate.

Kovacs, J A, Allegra, C J, Swan, J C, Drake, J C, Parrillo, J E, Chabner, B A, ...

Piritrexim, a lipid-soluble antifolate, was evaluated for its activity against Pneumocystis carinii and Toxoplasma gondii. The concentration of piritrexim needed to inhibit 50% of the catalytic...

Purification, toxicity, and antiendotoxin activity of polymyxin B nonapeptide.

Danner, R L, Joiner, K A, Rubin, M, Patterson, W H, Johnson, N, Ayers, K M, ...

Polymyxin B, a relatively toxic antibiotic, has potent endotoxin-neutralizing properties that may be beneficial as adjunctive therapy in gram-negative sepsis. Polymyxin B nonapeptide (deacylated...

Histoenzymological study of selected dehydrogenase enzymes in Pneumocystis carinii.

Mazer, M A, Kovacs, J A, Swan, J C, Parrillo, J E, Masur, H

The metabolic activity of Pneumocystis carinii cysts was studied histochemically by a tetrazolium dye technique to assess substrate-specific dehydrogenase activity. Lactate dehydrogenase, succinate...

Detection of Pneumocystis carinii by fluorescent-antibody stain using a combination of three monoclonal antibodies.

Gill, V J, Evans, G, Stock, F, Parrillo, J E, Masur, H, Kovacs, J A

A combination of three monoclonal antibodies, two prepared against human and one against rat Pneumocystis carinii, was used in an indirect fluorescent-antibody stain (IFA) to diagnose P. carinii in...

Role of endotoxemia in cardiovascular dysfunction and mortality. Escherichia coli and Staphylococcus aureus challenges in a canine model of human septic shock.

Natanson, C, Danner, R L, Elin, R J, Hosseini, J M, Peart, K W, Banks, S M, ...

Using different types of bacteria and a canine model simulating human septic shock, we investigated the role of endotoxin in cardiovascular dysfunction and mortality. Either Escherichia coli (a...

Bacterial lipopolysaccharides prime human neutrophils for enhanced production of leukotriene B4.

Doerfler, M E, Danner, R L, Shelhamer, J H, Parrillo, J E

Neutrophils can be "primed" for an enhanced respiratory burst by lipopolysaccharide (LPS) in concentrations measurable in patients with septic shock. Leukotriene B4 (LTB4) is the primary eicosanoid...

Gram-negative bacteremia produces both severe systolic and diastolic cardiac dysfunction in a canine model that simulates human septic shock.

Natanson, C, Fink, M P, Ballantyne, H K, MacVittie, T J, Conklin, J J, Parrillo, J E

A canine sepsis model that simulates the human cardiovascular response to septic shock was produced in 10 conscious unsedated dogs by implanting an Escherichia coli-infected clot into the peritoneum,...

A circulating myocardial depressant substance in humans with septic shock. Septic shock patients with a reduced ejection fraction have a circulating factor that depresses in vitro myocardial cell performance.

Parrillo, J E, Burch, C, Shelhamer, J H, Parker, M M, Natanson, C, Schuette, W

We have previously described a subpopulation of patients with septic shock who had a reversible depression of radionuclide-determined left ventricular ejection fraction (EF). To investigate the...

Inhibition of endotoxin-induced priming of human neutrophils by lipid X and 3-Aza-lipid X.

Danner, R L, Joiner, K A, Parrillo, J E

Lipid X, a precursor of lipid A (the toxic moiety of endotoxin), has been shown to protect animals from the lethal effects of endotoxin challenge. We investigated the mechanism of action of lipid X...

Bioavailability of oral trimetrexate in patients with acquired immunodeficiency syndrome.

Rogers, P, Allegra, C J, Murphy, R F, Drake, J C, Masur, H, Poplack, D G, ...

The combination of the lipophilic antifolate trimetrexate and the rescue agent leucovorin has shown promise in the treatment of Pneumocystis carinii pneumonia in patients with acquired...

Potent antipneumocystis and antitoxoplasma activities of piritrexim, a lipid-soluble antifolate.

Kovacs, J A, Allegra, C J, Swan, J C, Drake, J C, Parrillo, J E, Chabner, B A, ...

Piritrexim, a lipid-soluble antifolate, was evaluated for its activity against Pneumocystis carinii and Toxoplasma gondii. The concentration of piritrexim needed to inhibit 50% of the catalytic...

Purification, toxicity, and antiendotoxin activity of polymyxin B nonapeptide.

Danner, R L, Joiner, K A, Rubin, M, Patterson, W H, Johnson, N, Ayers, K M, ...

Polymyxin B, a relatively toxic antibiotic, has potent endotoxin-neutralizing properties that may be beneficial as adjunctive therapy in gram-negative sepsis. Polymyxin B nonapeptide (deacylated...

Histoenzymological study of selected dehydrogenase enzymes in Pneumocystis carinii.

Mazer, M A, Kovacs, J A, Swan, J C, Parrillo, J E, Masur, H

The metabolic activity of Pneumocystis carinii cysts was studied histochemically by a tetrazolium dye technique to assess substrate-specific dehydrogenase activity. Lactate dehydrogenase, succinate...

Detection of Pneumocystis carinii by fluorescent-antibody stain using a combination of three monoclonal antibodies.

Gill, V J, Evans, G, Stock, F, Parrillo, J E, Masur, H, Kovacs, J A

A combination of three monoclonal antibodies, two prepared against human and one against rat Pneumocystis carinii, was used in an indirect fluorescent-antibody stain (IFA) to diagnose P. carinii in...

Role of endotoxemia in cardiovascular dysfunction and mortality. Escherichia coli and Staphylococcus aureus challenges in a canine model of human septic shock.

Natanson, C, Danner, R L, Elin, R J, Hosseini, J M, Peart, K W, Banks, S M, ...

Using different types of bacteria and a canine model simulating human septic shock, we investigated the role of endotoxin in cardiovascular dysfunction and mortality. Either Escherichia coli (a...

Bacterial lipopolysaccharides prime human neutrophils for enhanced production of leukotriene B4.

Doerfler, M E, Danner, R L, Shelhamer, J H, Parrillo, J E

Neutrophils can be "primed" for an enhanced respiratory burst by lipopolysaccharide (LPS) in concentrations measurable in patients with septic shock. Leukotriene B4 (LTB4) is the primary eicosanoid...

Gram-negative bacteremia produces both severe systolic and diastolic cardiac dysfunction in a canine model that simulates human septic shock.

Natanson, C, Fink, M P, Ballantyne, H K, MacVittie, T J, Conklin, J J, Parrillo, J E

A canine sepsis model that simulates the human cardiovascular response to septic shock was produced in 10 conscious unsedated dogs by implanting an Escherichia coli-infected clot into the peritoneum,...

A circulating myocardial depressant substance in humans with septic shock. Septic shock patients with a reduced ejection fraction have a circulating factor that depresses in vitro myocardial cell performance.

Parrillo, J E, Burch, C, Shelhamer, J H, Parker, M M, Natanson, C, Schuette, W

We have previously described a subpopulation of patients with septic shock who had a reversible depression of radionuclide-determined left ventricular ejection fraction (EF). To investigate the...

Inhibition of endotoxin-induced priming of human neutrophils by lipid X and 3-Aza-lipid X.

Danner, R L, Joiner, K A, Parrillo, J E

Lipid X, a precursor of lipid A (the toxic moiety of endotoxin), has been shown to protect animals from the lethal effects of endotoxin challenge. We investigated the mechanism of action of lipid X...

Characterization of the direct effects of cyclophosphamide on cell-mediated immunological responses.

Balow, J E, Parrillo, J E, Fauci, A S

The direct immunosuppressive effects of cyclophosphamide were examined by incubating normal guinea-pig mononuclear cells with activated cyclophosphamide prepared from the serum of separate animals...

Apparent direct cellular cytoxicity mediated via cytophilic antibody: Multiple Fc receptor-bearing effector cell populations mediating cytophilic antibody induced cytoxicity

Parrillo, J. E., Fauci, A. S.

Guinea-pigs immunized with chicken red blood cells (CRBC) developed cytotoxic effector cells in peripheral blood, spleen, lymph nodes, bone marrow and peritoneal exudate cells. Although it appeared...

Mechanisms of corticosteroid action on lymphocyte subpopulations. III. Differential effects of dexamethasone administration on subpopulations of effector cells mediating cellular cytotoxicity in man

Parrillo, J. E., Fauci, A. S.

The present study investigated the effect of dexamethasone (DEX) administration on different populations of mononuclear cells and neutrophils mediating antibody-dependent cellular cytotoxicity (ADCC)...

Comparison of the relative cytotoxic effector cell capabilities and the proportions of cells bearing various surface markers in human tonsil and peripheral blood mononuclear cells.

Hunninghake, G W, Haynes, B F, Parrillo, J E, Fauci, A S

The relative cytotoxic effector cell capabilities and the proportions of cells bearing various surface markers in human tonsil and peripheral blood mononuclear cells has been studied. The peripheral...