Abstracts – Plenary Lectures – O-1 Oxygen, life and energy conversion (2009)
Oxygen evolving life may have begun on earth about 3,400 billion years ago, but the interpretation of geochemical evidence, for example in the Buck Reef Chert, is disputed. However, evidence from...
Designing Optimal AOQL Sampling Plans a Computerized Approach. (2002)
Walker,J. E., Leavenworth,R. S.
This study provides an algorithm and computer program, coded in Fortran IV, to develop double sampling acceptance plans for attributes which satisfy a specified Average Outgoing Quality Limit (AOQL)...
The structure of bovine mitochondrial F1-ATPase: an example of rotary catalysis (1999)
Leslie, A.G.W., Abrahams, J.P., Braig, K., Lutter, R., Menz, R.I., Orriss, G.L., ...
Manejo Conductual: Un Enfoque Práctico Para Educadores (1987)
Traducción de: Behavior Management
Traducción de: Behavior Management
Part of the atp (or unc) operon encoding the α, β, γ, δ and ϵ subunits of Escherichia coli ATP-synthase has been cloned into the plasmid pACYC184. The DNA coding for the largest of these...
Walker, J. E., Albers, James W., Tourtellotte, Wallace W., Henderson, W. G., Potvin, A. R., Smith, A.
A double-blind crossover trial of amantadine vs. placebo was carried out involving 42 patients with Parkinson's disease: 64 per cent of the patients on amantadine experienced subjective improvement...
The structure of bovine F1-ATPase complexed with the peptide antibiotic efrapeptin.
Abrahams, J P, Buchanan, S K, Van Raaij, M J, Fearnley, I M, Leslie, A G, Walker, J E
In the previously determined structure of mitochondrial F1-ATPase determined with crystals grown in the presence of adenylyl-imidodiphosphate (AMP-PNP) and ADP, the three catalytic beta-subunits have...
The structure of bovine F1-ATPase complexed with the antibiotic inhibitor aurovertin B.
Van Raaij, M J, Abrahams, J P, Leslie, A G, Walker, J E
In the structure of bovine mitochondrial F1-ATPase that was previously determined with crystals grown in the presence of adenylyl-imidodiphosphate (AMP-PNP) and ADP, the three catalytic beta-subunits...
Citrin and aralar1 are Ca2+-stimulated aspartate/glutamate transporters in mitochondria
Palmieri, L., Pardo, B., Lasorsa, F.M., Del Arco, A., Kobayashi, K., Iijima, M., ...
The mitochondrial aspartate/glutamate carrier catalyzes an important step in both the urea cycle and the aspartate/malate NADH shuttle. Citrin and aralar1 are homologous proteins belonging to the...
The structure of bovine IF1, the regulatory subunit of mitochondrial F-ATPase
Cabezón, E., Runswick, M.J., Leslie, A.G.W., Walker, J.E.
In mitochondria, the hydrolytic activity of ATP synthase is regulated by an inhibitor protein, IF1. Its binding to ATP synthase depends on pH, and below neutrality, IF1 is dimeric and forms a stable...
Identification and reconstitution of theyeast mitochondrial transporter for thiamine pyrophosphate
Marobbio, C.M.T., Vozza, A., Harding, M., Bisaccia, F., Palmieri, F., Walker, J.E.
The genome of Saccharomyces cerevisiae contains 35 members of a family of transport proteins that, with a single exception, are found in the inner membranes of mitochondria. The transport functions...
Goedert, M, Wischik, C M, Crowther, R A, Walker, J E, Klug, A
Screening of cDNA libraries prepared from the frontal cortex of an Alzheimer disease patient and from fetal human brain has led to isolation of the cDNA for a core protein of the paired helical...
Wischik, C M, Novak, M, Thøgersen, H C, Edwards, P C, Runswick, M J, Jakes, R, ...
A substantially enriched preparation of Alzheimer paired helical filaments (PHFs) has been used as a starting point for biochemical studies. Pronase treatment, which strips off adhering proteins,...
Part of the atp (or unc) operon encoding the alpha, beta, gamma, delta, and epsilon subunits of Escherichia coli ATP-synthase has been cloned into the plasmid pACYC 184. The DNA coding for the...
Saraste, M, Gay, N J, Eberle, A, Runswick, M J, Walker, J E
The nucleotide sequence of the promoter distal region of the atp (or unc) operon of Escherichia coli has been determined. It encodes the gamma, beta and epsilon subunits of the ATP-synthase complex...
Fearnley, I M, Runswick, M J, Walker, J E
The mitochondrial NADH-ubiquinone reductase (complex I) is an assembly of approximately 26 different polypeptides. In vertebrates and invertebrates, seven of its subunits are the products of genes in...
Inherent asymmetry of the structure of F1-ATPase from bovine heart mitochondria at 6.5 A resolution.
Abrahams, J P, Lutter, R, Todd, R J, Van Raaij, M J, Leslie, A G, Walker, J E
ATP synthase, the assembly which makes ATP in mitochondria, chloroplasts and bacteria, uses transmembrane proton gradients generated by respiration or photosynthesis to drive the phosphorylation of...
Walker, J E, Saraste, M, Runswick, M J, Gay, N J
The alpha- and beta-subunits of membrane-bound ATP synthase complex bind ATP and ADP: beta contributes to catalytic sites, and alpha may be involved in regulation of ATP synthase activity. The...
Runswick, M J, Powell, S J, Nyren, P, Walker, J E
A cDNA encoding the precursor of the bovine mitochondrial phosphate carrier protein has been cloned from a bovine cDNA library using a mixture of 128 different 17-mer oligonucleotides as...
Two cDNAs encoding different precursor proteins of the same mature proteolipid subunit of mitochondrial ATP synthase have been cloned from a bovine cDNA library. The hybridisation probe was a mixture...
Runswick, M J, Medd, S M, Walker, J E
The delta-subunit of ATP synthase from bovine heart mitochondria is part of the extrinsic membrane domain, F1-ATPase. The mature protein is 146 amino acids in length and its function is obscure. It...
Fearnley, I M, Walker, J E, Martinus, R D, Jolly, R D, Kirkland, K B, Shaw, G J, ...
The ceroid lipofuscinoses are a group of neurodegenerative lysosomal storage diseases of children and animals that are recessively inherited. In diseased individuals fluorescent storage bodies...
Somers, D O, Medd, S M, Walker, J E, Adams, M J
Sheep liver 6-phosphogluconate dehydrogenase (6-PGDH) is an enzyme of the pentose phosphate pathway. Evidence has appeared which suggests that the 6-PGDH protein sequence determined previously by...
Sequences of members of the human gene family for the c subunit of mitochondrial ATP synthase.
Subunit c is an intrinsic membrane component of ATP synthase, and in mammals it is encoded by two expressed nuclear genes, P1 and P2. Both genes encode the same mature c subunit, but the...
Medd, S M, Walker, J E, Jolly, R D
The human and bovine genomes each contain two expressed nuclear genes, called P1 and P2, for subunit c, a hydrophobic subunit of the membrane sector, Fo, of mitochondrial ATP synthase. Both P1 and P2...
Van Walraven, H S, Lutter, R, Walker, J E
The sequences of the genes for the nine subunits of ATP synthase in the thermophilic cyanobacterium Synechococcus 6716 have been determined. The genes were identified by comparison of the encoded...
Fiermonte, G, Walker, J E, Palmieri, F
The oxoglutarate carrier, an intrinsic membrane-transport protein of the inner membranes of bovine-heart mitochondria, has been expressed at an abundant level in Escherichia coli. It accumulates in...
Lutter, R, Saraste, M, Van Walraven, H S, Runswick, M J, Finel, M, Deatherage, J F, ...
A new procedure for the isolation of ATP synthase from bovine mitochondria has been developed, with the primary objective of producing enzyme suitable for crystallization trials. Proteins were...
A region was cloned from the genome of the purple non-sulphur photobacterium Rhodospirillum rubrum that contains genes coding for the membrane protein subunits of the F0 sector of ATP synthase. The...
Viñas, O, Powell, S J, Runswick, M J, Iacobazzi, V, Walker, J E
The epsilon-subunit of ATP synthase from bovine heart mitochondria is assembled into the extrinsic membrane sector, F1-ATPase. The mature protein is 50 amino acid residues in length and its function...
Collinson, I R, Fearnley, I M, Skehel, J M, Runswick, M J, Walker, J E
The exposure to trypsinolysis of subunits of F1F0-ATPase and of its F0 domain have been compared in everted inner membrane vesicles (submitochondrial particles) made from bovine mitochondria....
Dyer, M R, Gay, N J, Walker, J E
The dicyclohexylcarbodi-imide-reactive proteolipid is a membrane subunit of mitochondrial ATP synthase. In cows it is encoded by two different nuclear genes known as P1 and P2. These genes are...
Walker, J E, Gay, N J, Saraste, M, Eberle, A N
The nucleotide sequence is described of a region of the Escherichia coli chromosome extending from oriC to phoS that also includes the loci gid, unc and glmS. Taken with known sequences for asnA and...
Molecular cloning of a bovine cathepsin.
A cDNA clone for a thiol endoproteinase has been isolated from a bovine heart cDNA library by using a mixture of 32 synthetic oligonucleotides as a hybridization probe. The inserted region is 672...
Nucleotide sequence of the Rhodospirillum rubrum atp operon.
Falk, G, Hampe, A, Walker, J E
The nucleotide sequence was determined of a 8775-base-pair region of DNA cloned from the photosynthetic non-sulphur bacterium Rhodospirillum rubrum. It contains a cluster of five genes encoding...
Transcription of Rhodospirillum rubrum atp operon.
The photosynthetic non-sulphur bacterium Rhodospirillum rubrum contains a cluster of five genes encoding the subunits of F1-ATPase [Falk, Hampe & Walker (1985) Biochem. J. 228, 391-407]....
Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator.
Gay, N J, Tybulewicz, V L, Walker, J E
The transposon Tn7 is unusual as it transposes at high frequencies from episomal elements to a unique site in the Escherichia coli chromosome. This unique site is within a region of dyad symmetry...
The frayed N-terminal of the inhibitor protein of bovine mitochondrial F1-ATPase.
Runswick, M J, Walker, J E, Gibson, B W, Williams, D H
The N-terminal region of the bovine mitochondrial F1-ATPase inhibitor protein is frayed. In three independently isolated samples about 62% of chains start at glycine-1. A further 22% of chains start...
Hayes, P K, Walsby, A E, Walker, J E
Gas vesicles of cyanobacteria are formed by a protein called 'gas-vesicle protein' (GVP). The complete amino acid sequence has been determined of GVP from Anabaena flos-aquae. It is 70 residues long...
The nucleotide sequence has been determined of a segment of 4680 bases of the pea chloroplast genome. It adjoins a sequence described elsewhere that encodes subunits of the F0 membrane domain of the...
A rapid solid-phase protein microsequencer.
Walker, J E, Fearnley, I M, Blows, R A
A solid-phase protein microsequencer is described that has been designed to determine protein sequences with subnanomolar quantities of protein. Its utility has been demonstrated by the determination...
Mason, R W, Walker, J E, Northrop, F D
Human liver cathepsin L consists of a heavy chain and a light chain with Mr values of 25,000 and 5000 respectively. The chains have been purified and their N-terminal amino acid sequences have been...
Expression of a gene encoding a novel ferredoxin in the cyanobacterium Synechococcus 6301.
A gene was discovered in the cyanobacterium Synechococcus 6301 that encodes a protein highly related to members of the [2Fe-2S] ferredoxin family found in chloroplasts and cyanobacteria. It follows a...
Dupuis, A, Skehel, J M, Walker, J E
The sequence of a 19 kDa subunit of NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria has been determined by a new strategy based on the polymerase chain reaction. The...
The gas vesicle of the planktonic cyanobacterium Anabaena flos-aquae is a cylindrical shell made of protein enclosing a gas-filled space. Protein sequence analysis shows that the vesicle is made from...
Purification and properties of D-ribulokinase and D-xylulokinase from Klebsiella aerogenes.
Neuberger, M S, Hartley, B S, Walker, J E
The D-ribulokinase and D-xylulokinase of Klebsiella aerogenes were purified to homogeneity from Escherichia coli K12 construct strains that synthesized these enzymes constitutively. The...
Two overlapping genes in bovine mitochondrial DNA encode membrane components of ATP synthase.
Two hydrophobic proteins have been purified to homogeneity from a mixture of about 13 proteins that are extracted from bovine mitochondria with a chloroform:methanol mixture. Sequence analysis shows...
Isolation of bacilysin and a new amino acid from culture filtrates of Bacillus subtilis
1. Bacilysin, a labile dipeptide antibiotic that lyses growing staphylococci, was isolated from culture fluids of Bacillus subtilis by a process giving higher yields than those previously obtained....
The structure of bacilysin and other products of Bacillus subtillis
1. Mass spectra of the trimethylsilyl derivative and the methyl ester of the N-trifluoroacetyl derivative of bacilysin indicated that the antibiotic had a molecular weight of 270. Several peaks in...
The delta- and epsilon-subunits of bovine F1-ATPase interact to form a heterodimeric subcomplex.
Orriss, G L, Runswick, M J, Collinson, I R, Miroux, B, Fearnley, I M, Skehel, J M, ...
The delta-subunit of bovine F1-ATPase was expressed from a bacterial vector at fairly high level in Escherichia coli, but the yield of bovine epsilon-subunit was rather low under similar conditions....
A new chromatographic procedure has been developed for the isolation of F1F0-ATPase and NADH:ubiquinone oxidoreductase (complex I) from a single batch of bovine heart mitochondria. The method...
A highly purified and monodisperse preparation of proton-translocating F1F0-ATPase from bovine heart mitochondria is an assembly of 16 unlike polypeptides. This preparation has been reconstituted in...
Fiermonte, G, Dolce, V, Arrigoni, R, Runswick, M J, Walker, J E, Palmieri, F
The dicarboxylate carrier (DIC) is a nuclear-encoded protein located in the mitochondrial inner membrane. It catalyses the transport of dicarboxylates such as malate and succinate across the...
Fearnley, I M, Finel, M, Skehel, J M, Walker, J E
The 39 kDa and 42 kDa subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria are nuclear-coded components of the hydrophobic protein fraction of the enzyme. Their amino acid...
The structure of bovine F1-ATPase complexed with the peptide antibiotic efrapeptin.
Abrahams, J P, Buchanan, S K, Van Raaij, M J, Fearnley, I M, Leslie, A G, Walker, J E
In the previously determined structure of mitochondrial F1-ATPase determined with crystals grown in the presence of adenylyl-imidodiphosphate (AMP-PNP) and ADP, the three catalytic beta-subunits have...
The structure of bovine F1-ATPase complexed with the antibiotic inhibitor aurovertin B.
Van Raaij, M J, Abrahams, J P, Leslie, A G, Walker, J E
In the structure of bovine mitochondrial F1-ATPase that was previously determined with crystals grown in the presence of adenylyl-imidodiphosphate (AMP-PNP) and ADP, the three catalytic beta-subunits...
Citrin and aralar1 are Ca2+-stimulated aspartate/glutamate transporters in mitochondria
Palmieri, L., Pardo, B., Lasorsa, F.M., Del Arco, A., Kobayashi, K., Iijima, M., ...
The mitochondrial aspartate/glutamate carrier catalyzes an important step in both the urea cycle and the aspartate/malate NADH shuttle. Citrin and aralar1 are homologous proteins belonging to the...
The structure of bovine IF1, the regulatory subunit of mitochondrial F-ATPase
Cabezón, E., Runswick, M.J., Leslie, A.G.W., Walker, J.E.
In mitochondria, the hydrolytic activity of ATP synthase is regulated by an inhibitor protein, IF1. Its binding to ATP synthase depends on pH, and below neutrality, IF1 is dimeric and forms a stable...
Identification and reconstitution of theyeast mitochondrial transporter for thiamine pyrophosphate
Marobbio, C.M.T., Vozza, A., Harding, M., Bisaccia, F., Palmieri, F., Walker, J.E.
The genome of Saccharomyces cerevisiae contains 35 members of a family of transport proteins that, with a single exception, are found in the inner membranes of mitochondria. The transport functions...
Goedert, M, Wischik, C M, Crowther, R A, Walker, J E, Klug, A
Screening of cDNA libraries prepared from the frontal cortex of an Alzheimer disease patient and from fetal human brain has led to isolation of the cDNA for a core protein of the paired helical...
Wischik, C M, Novak, M, Thøgersen, H C, Edwards, P C, Runswick, M J, Jakes, R, ...
A substantially enriched preparation of Alzheimer paired helical filaments (PHFs) has been used as a starting point for biochemical studies. Pronase treatment, which strips off adhering proteins,...
Part of the atp (or unc) operon encoding the alpha, beta, gamma, delta, and epsilon subunits of Escherichia coli ATP-synthase has been cloned into the plasmid pACYC 184. The DNA coding for the...
Saraste, M, Gay, N J, Eberle, A, Runswick, M J, Walker, J E
The nucleotide sequence of the promoter distal region of the atp (or unc) operon of Escherichia coli has been determined. It encodes the gamma, beta and epsilon subunits of the ATP-synthase complex...
Fearnley, I M, Runswick, M J, Walker, J E
The mitochondrial NADH-ubiquinone reductase (complex I) is an assembly of approximately 26 different polypeptides. In vertebrates and invertebrates, seven of its subunits are the products of genes in...
Inherent asymmetry of the structure of F1-ATPase from bovine heart mitochondria at 6.5 A resolution.
Abrahams, J P, Lutter, R, Todd, R J, Van Raaij, M J, Leslie, A G, Walker, J E
ATP synthase, the assembly which makes ATP in mitochondria, chloroplasts and bacteria, uses transmembrane proton gradients generated by respiration or photosynthesis to drive the phosphorylation of...
Walker, J E, Saraste, M, Runswick, M J, Gay, N J
The alpha- and beta-subunits of membrane-bound ATP synthase complex bind ATP and ADP: beta contributes to catalytic sites, and alpha may be involved in regulation of ATP synthase activity. The...
Runswick, M J, Powell, S J, Nyren, P, Walker, J E
A cDNA encoding the precursor of the bovine mitochondrial phosphate carrier protein has been cloned from a bovine cDNA library using a mixture of 128 different 17-mer oligonucleotides as...
Two cDNAs encoding different precursor proteins of the same mature proteolipid subunit of mitochondrial ATP synthase have been cloned from a bovine cDNA library. The hybridisation probe was a mixture...
Runswick, M J, Medd, S M, Walker, J E
The delta-subunit of ATP synthase from bovine heart mitochondria is part of the extrinsic membrane domain, F1-ATPase. The mature protein is 146 amino acids in length and its function is obscure. It...
Fearnley, I M, Walker, J E, Martinus, R D, Jolly, R D, Kirkland, K B, Shaw, G J, ...
The ceroid lipofuscinoses are a group of neurodegenerative lysosomal storage diseases of children and animals that are recessively inherited. In diseased individuals fluorescent storage bodies...
Somers, D O, Medd, S M, Walker, J E, Adams, M J
Sheep liver 6-phosphogluconate dehydrogenase (6-PGDH) is an enzyme of the pentose phosphate pathway. Evidence has appeared which suggests that the 6-PGDH protein sequence determined previously by...
Sequences of members of the human gene family for the c subunit of mitochondrial ATP synthase.
Subunit c is an intrinsic membrane component of ATP synthase, and in mammals it is encoded by two expressed nuclear genes, P1 and P2. Both genes encode the same mature c subunit, but the...
Medd, S M, Walker, J E, Jolly, R D
The human and bovine genomes each contain two expressed nuclear genes, called P1 and P2, for subunit c, a hydrophobic subunit of the membrane sector, Fo, of mitochondrial ATP synthase. Both P1 and P2...
Van Walraven, H S, Lutter, R, Walker, J E
The sequences of the genes for the nine subunits of ATP synthase in the thermophilic cyanobacterium Synechococcus 6716 have been determined. The genes were identified by comparison of the encoded...
Fiermonte, G, Walker, J E, Palmieri, F
The oxoglutarate carrier, an intrinsic membrane-transport protein of the inner membranes of bovine-heart mitochondria, has been expressed at an abundant level in Escherichia coli. It accumulates in...
Lutter, R, Saraste, M, Van Walraven, H S, Runswick, M J, Finel, M, Deatherage, J F, ...
A new procedure for the isolation of ATP synthase from bovine mitochondria has been developed, with the primary objective of producing enzyme suitable for crystallization trials. Proteins were...
A region was cloned from the genome of the purple non-sulphur photobacterium Rhodospirillum rubrum that contains genes coding for the membrane protein subunits of the F0 sector of ATP synthase. The...
Viñas, O, Powell, S J, Runswick, M J, Iacobazzi, V, Walker, J E
The epsilon-subunit of ATP synthase from bovine heart mitochondria is assembled into the extrinsic membrane sector, F1-ATPase. The mature protein is 50 amino acid residues in length and its function...
Collinson, I R, Fearnley, I M, Skehel, J M, Runswick, M J, Walker, J E
The exposure to trypsinolysis of subunits of F1F0-ATPase and of its F0 domain have been compared in everted inner membrane vesicles (submitochondrial particles) made from bovine mitochondria....
Dyer, M R, Gay, N J, Walker, J E
The dicyclohexylcarbodi-imide-reactive proteolipid is a membrane subunit of mitochondrial ATP synthase. In cows it is encoded by two different nuclear genes known as P1 and P2. These genes are...
Walker, J E, Gay, N J, Saraste, M, Eberle, A N
The nucleotide sequence is described of a region of the Escherichia coli chromosome extending from oriC to phoS that also includes the loci gid, unc and glmS. Taken with known sequences for asnA and...
Molecular cloning of a bovine cathepsin.
A cDNA clone for a thiol endoproteinase has been isolated from a bovine heart cDNA library by using a mixture of 32 synthetic oligonucleotides as a hybridization probe. The inserted region is 672...
Nucleotide sequence of the Rhodospirillum rubrum atp operon.
Falk, G, Hampe, A, Walker, J E
The nucleotide sequence was determined of a 8775-base-pair region of DNA cloned from the photosynthetic non-sulphur bacterium Rhodospirillum rubrum. It contains a cluster of five genes encoding...
Transcription of Rhodospirillum rubrum atp operon.
The photosynthetic non-sulphur bacterium Rhodospirillum rubrum contains a cluster of five genes encoding the subunits of F1-ATPase [Falk, Hampe & Walker (1985) Biochem. J. 228, 391-407]....
Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator.
Gay, N J, Tybulewicz, V L, Walker, J E
The transposon Tn7 is unusual as it transposes at high frequencies from episomal elements to a unique site in the Escherichia coli chromosome. This unique site is within a region of dyad symmetry...
The frayed N-terminal of the inhibitor protein of bovine mitochondrial F1-ATPase.
Runswick, M J, Walker, J E, Gibson, B W, Williams, D H
The N-terminal region of the bovine mitochondrial F1-ATPase inhibitor protein is frayed. In three independently isolated samples about 62% of chains start at glycine-1. A further 22% of chains start...
Hayes, P K, Walsby, A E, Walker, J E
Gas vesicles of cyanobacteria are formed by a protein called 'gas-vesicle protein' (GVP). The complete amino acid sequence has been determined of GVP from Anabaena flos-aquae. It is 70 residues long...
The nucleotide sequence has been determined of a segment of 4680 bases of the pea chloroplast genome. It adjoins a sequence described elsewhere that encodes subunits of the F0 membrane domain of the...
A rapid solid-phase protein microsequencer.
Walker, J E, Fearnley, I M, Blows, R A
A solid-phase protein microsequencer is described that has been designed to determine protein sequences with subnanomolar quantities of protein. Its utility has been demonstrated by the determination...
Mason, R W, Walker, J E, Northrop, F D
Human liver cathepsin L consists of a heavy chain and a light chain with Mr values of 25,000 and 5000 respectively. The chains have been purified and their N-terminal amino acid sequences have been...
Expression of a gene encoding a novel ferredoxin in the cyanobacterium Synechococcus 6301.
A gene was discovered in the cyanobacterium Synechococcus 6301 that encodes a protein highly related to members of the [2Fe-2S] ferredoxin family found in chloroplasts and cyanobacteria. It follows a...
Dupuis, A, Skehel, J M, Walker, J E
The sequence of a 19 kDa subunit of NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria has been determined by a new strategy based on the polymerase chain reaction. The...
Fearnley, I M, Finel, M, Skehel, J M, Walker, J E
The 39 kDa and 42 kDa subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria are nuclear-coded components of the hydrophobic protein fraction of the enzyme. Their amino acid...
The gas vesicle of the planktonic cyanobacterium Anabaena flos-aquae is a cylindrical shell made of protein enclosing a gas-filled space. Protein sequence analysis shows that the vesicle is made from...
Purification and properties of D-ribulokinase and D-xylulokinase from Klebsiella aerogenes.
Neuberger, M S, Hartley, B S, Walker, J E
The D-ribulokinase and D-xylulokinase of Klebsiella aerogenes were purified to homogeneity from Escherichia coli K12 construct strains that synthesized these enzymes constitutively. The...
Two overlapping genes in bovine mitochondrial DNA encode membrane components of ATP synthase.
Two hydrophobic proteins have been purified to homogeneity from a mixture of about 13 proteins that are extracted from bovine mitochondria with a chloroform:methanol mixture. Sequence analysis shows...
Isolation of bacilysin and a new amino acid from culture filtrates of Bacillus subtilis
1. Bacilysin, a labile dipeptide antibiotic that lyses growing staphylococci, was isolated from culture fluids of Bacillus subtilis by a process giving higher yields than those previously obtained....
The structure of bacilysin and other products of Bacillus subtillis
1. Mass spectra of the trimethylsilyl derivative and the methyl ester of the N-trifluoroacetyl derivative of bacilysin indicated that the antibiotic had a molecular weight of 270. Several peaks in...
The delta- and epsilon-subunits of bovine F1-ATPase interact to form a heterodimeric subcomplex.
Orriss, G L, Runswick, M J, Collinson, I R, Miroux, B, Fearnley, I M, Skehel, J M, ...
The delta-subunit of bovine F1-ATPase was expressed from a bacterial vector at fairly high level in Escherichia coli, but the yield of bovine epsilon-subunit was rather low under similar conditions....
A new chromatographic procedure has been developed for the isolation of F1F0-ATPase and NADH:ubiquinone oxidoreductase (complex I) from a single batch of bovine heart mitochondria. The method...
A highly purified and monodisperse preparation of proton-translocating F1F0-ATPase from bovine heart mitochondria is an assembly of 16 unlike polypeptides. This preparation has been reconstituted in...
Fiermonte, G, Dolce, V, Arrigoni, R, Runswick, M J, Walker, J E, Palmieri, F
The dicarboxylate carrier (DIC) is a nuclear-encoded protein located in the mitochondrial inner membrane. It catalyses the transport of dicarboxylates such as malate and succinate across the...
Structural model of F1-ATPase and the implications for rotary catalysis.
The crystal structure of bovine mitochondrial F1-ATPase is described. Several features of the structure are consistent with the binding change mechanism of catalysis, in which binding of substrates...