Fujiwara, S, Hashiba, H, Hirota, T, Forstner, J F
We have examined the competitive binding of several species of Bifidobacterium and Escherichia coli Pb176, an enterotoxigenic E. coli (ETEC) strain, to gangliotetraosylceramide (asialo GM1 or GA1), a...
Burkholderia (basonym Pseudomonas) cepacia binding to lipid receptors.
Sylvester, F A, Sajjan, U S, Forstner, J F
Piliated Burkholderia (formerly Pseudomonas) cepacia from sputa of cys tic fibrosis patients in Toronto, Canada, were shown earlier to bind to purified mucins and to a protein receptor on epithelial...
Sylvester, F A, Philpott, D, Gold, B, Lastovica, A, Forstner, J F
Campylobacter upsaliensis is a recently recognized human enteric pathogen associated with enteritis, colitis, bacteremia, and sepsis. Very little is known about the mechanisms of pathogenesis of this...
Sajjan, U S, Sun, L, Goldstein, R, Forstner, J F
Previous studies have shown that appendage pili of Burkholderia cepacia strains isolated from patients with cystic fibrosis (CF) at The Hospital for Sick Children, Toronto, Canada, mediate adherence...
Goldstein, R, Sun, L, Jiang, R Z, Sajjan, U, Forstner, J F, Campanelli, C
One or more of five morphologically distinct classes of appendage pili were determined to be peritrichously expressed by Burkholderia (formerly Pseudomonas) cepacia isolated from disparate sources....
In previous experiments, we have shown that isolates of Pseudomonas cepacia from sputa of patients with cystic fibrosis (CF), particularly those with severe lung infection, exhibited specific binding...
Purified rat intestinal mucin was used as a model mucin to study the binding of Escherichia coli serotype O157:H7, a human pathogen associated with outbreaks of hemorrhagic colitis and hemolytic...
Purified rat intestinal mucin was used to identify mucin-binding sites for type 1-piliated Escherichia coli O157:H7 strain CL-49 isolated from a patient with hemorrhagic colitis and hemolytic uremic...
Role of a 22-kilodalton pilin protein in binding of Pseudomonas cepacia to buccal epithelial cells.
Previously we have shown that many isolates of Pseudomonas cepacia obtained from the sputum of patients with cystic fibrosis exhibit specific binding to purified mucins. The binding was mediated by a...
Qureshi, R, Forstner, G G, Forstner, J F
We have developed a double-antibody radioimmunoassay for the quantitative measurement of human goblet cell mucin (GCM) in order to study intestinal mucus in human and other species. The assay used...
Sajjan, U S, Corey, M, Karmali, M A, Forstner, J F
Although not as prevalent as Pseudomonas aeruginosa, Pseudomonas cepacia is another opportunistic pathogen which colonizes the lungs of at least some patients with cystic fibrosis. A subgroup of...
McCool, D J, Marcon, M A, Forstner, J F, Forstner, G G
The T84 colonic adenocarcinoma cell line, which has been used extensively as a model for studies of epithelial chloride secretion, also produces mucin and secretes it in culture. Electron microscopy...
Tissue-specific expression of a rat intestinal mucin-like peptide.
Xu, G, Wang, D, Huan, L J, Cutz, E, Forstner, G G, Forstner, J F
Expression of the gene for a rat intestinal mucin-like peptide (MLP) was studied by Northern-blot analyses of RNA prepared from a panel of rat tissues. Four probes (A-D) were constructed so as to...
Suggestive evidence for two different mucin genes in rat intestine.
Khatri, I A, Forstner, G G, Forstner, J F
In the present report we describe the isolation and sequence of a partial cDNA (M2-798) for a rat intestinal mucin designated M2. A rat intestinal lambda ZAP II cDNA library was screened using a...
McCool, D J, Forstner, J F, Forstner, G G
We have shown previously [McCool, Forstner and Forstner (1994) Biochem. J. 302, 111-118] using pulse-chase labelling of mucin with [3H]threonine that LS180 colonic tumour cells synthesize and secrete...
Synthesis and secretion of mucin by the human colonic tumour cell line LS180.
McCool, D J, Forstner, J F, Forstner, G G
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media...
Roberton, A M, Mantle, M, Fahim, R E, Specian, R D, Bennick, A, Kawagishi, S, ...
The existence of a discrete 'link' peptide in epithelial mucins has been debated for many years. There is evidence that at least some mucins contain a specific 'link' peptide (or glycopeptide) that...
Mantle, M, Forstner, G G, Forstner, J F
Previous studies have shown that human small-intestinal mucin consists of high-Mr glycoproteins and a smaller S-S-bonded protein of 118 kDa. The major antigenic determinants of the mucin were...
Characterization and localization of the putative 'link' component in rat small-intestinal mucin.
Fahim, R E, Specian, R D, Forstner, G G, Forstner, J F
Rat intestinal mucin is polymerized by a putative 'link' component of Mr 118,000 that can be released from the native mucin by thiol reduction [Fahim, Forstner & Forstner (1983) Biochem. J. 209,...
Fahim, R E, Forstner, G G, Forstner, J F
An investigation was undertaken to discover whether mucin purified from secretions in the lumen of rat small intestine differed in structure or composition from intracellular mucin purified from rat...
Antigenic and structural features of goblet-cell mucin of human small intestine.
Mantle, M, Forstner, G G, Forstner, J F
With the use of a newly developed solid-phase radioimmunoassay method, the major antigenic determinants of human small-intestinal goblet-cell mucin were investigated and related to the overall...
Heterogeneity of rat goblet-cell mucin before and after reduction.
Fahim, R E, Forstner, G G, Forstner, J F
Goblet-cell mucin of rat small intestine was purified from mucosal scrapings by using centrifugation, Sepharose 4B and Sepharose 2B chromatography. The mucin was applied in low concentrations (1...
Enhancement of the viscosity of mucin by serum albumin.
List, S J, Findlay, B P, Forstner, G G, Forstner, J F
The interaction of serum albumin with a model epithelial mucin from pig stomach was explored by rotary viscometry. During 30 min of incubation of human serum albumin(20mg/ml) and pig gastric mucin...
Khatri, I A, Forstner, G G, Forstner, J F
The present study reveals that partial proteolytic degradation of rat Muc 2 mucin can occur rapidly even in the presence of a battery of proteinase inhibitors. During the initial steps of...
McCool, D J, Okada, Y, Forstner, J F, Forstner, G G
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to...
Bell, S L, Xu, G, Forstner, J F
DNA constructs based on the 534-amino-acid C-terminus of rat mucin protein Muc2 (RMC), were transfected into COS cells and the resultant (35)S-labelled dimers and monomers were detected by SDS/PAGE...
Fujiwara, S, Hashiba, H, Hirota, T, Forstner, J F
We have examined the competitive binding of several species of Bifidobacterium and Escherichia coli Pb176, an enterotoxigenic E. coli (ETEC) strain, to gangliotetraosylceramide (asialo GM1 or GA1), a...
Burkholderia (basonym Pseudomonas) cepacia binding to lipid receptors.
Sylvester, F A, Sajjan, U S, Forstner, J F
Piliated Burkholderia (formerly Pseudomonas) cepacia from sputa of cys tic fibrosis patients in Toronto, Canada, were shown earlier to bind to purified mucins and to a protein receptor on epithelial...
Sylvester, F A, Philpott, D, Gold, B, Lastovica, A, Forstner, J F
Campylobacter upsaliensis is a recently recognized human enteric pathogen associated with enteritis, colitis, bacteremia, and sepsis. Very little is known about the mechanisms of pathogenesis of this...
Sajjan, U S, Sun, L, Goldstein, R, Forstner, J F
Previous studies have shown that appendage pili of Burkholderia cepacia strains isolated from patients with cystic fibrosis (CF) at The Hospital for Sick Children, Toronto, Canada, mediate adherence...
Goldstein, R, Sun, L, Jiang, R Z, Sajjan, U, Forstner, J F, Campanelli, C
One or more of five morphologically distinct classes of appendage pili were determined to be peritrichously expressed by Burkholderia (formerly Pseudomonas) cepacia isolated from disparate sources....
In previous experiments, we have shown that isolates of Pseudomonas cepacia from sputa of patients with cystic fibrosis (CF), particularly those with severe lung infection, exhibited specific binding...
Purified rat intestinal mucin was used as a model mucin to study the binding of Escherichia coli serotype O157:H7, a human pathogen associated with outbreaks of hemorrhagic colitis and hemolytic...
Purified rat intestinal mucin was used to identify mucin-binding sites for type 1-piliated Escherichia coli O157:H7 strain CL-49 isolated from a patient with hemorrhagic colitis and hemolytic uremic...
Role of a 22-kilodalton pilin protein in binding of Pseudomonas cepacia to buccal epithelial cells.
Previously we have shown that many isolates of Pseudomonas cepacia obtained from the sputum of patients with cystic fibrosis exhibit specific binding to purified mucins. The binding was mediated by a...
Qureshi, R, Forstner, G G, Forstner, J F
We have developed a double-antibody radioimmunoassay for the quantitative measurement of human goblet cell mucin (GCM) in order to study intestinal mucus in human and other species. The assay used...
Sajjan, U S, Corey, M, Karmali, M A, Forstner, J F
Although not as prevalent as Pseudomonas aeruginosa, Pseudomonas cepacia is another opportunistic pathogen which colonizes the lungs of at least some patients with cystic fibrosis. A subgroup of...
McCool, D J, Marcon, M A, Forstner, J F, Forstner, G G
The T84 colonic adenocarcinoma cell line, which has been used extensively as a model for studies of epithelial chloride secretion, also produces mucin and secretes it in culture. Electron microscopy...
Tissue-specific expression of a rat intestinal mucin-like peptide.
Xu, G, Wang, D, Huan, L J, Cutz, E, Forstner, G G, Forstner, J F
Expression of the gene for a rat intestinal mucin-like peptide (MLP) was studied by Northern-blot analyses of RNA prepared from a panel of rat tissues. Four probes (A-D) were constructed so as to...
Suggestive evidence for two different mucin genes in rat intestine.
Khatri, I A, Forstner, G G, Forstner, J F
In the present report we describe the isolation and sequence of a partial cDNA (M2-798) for a rat intestinal mucin designated M2. A rat intestinal lambda ZAP II cDNA library was screened using a...
McCool, D J, Forstner, J F, Forstner, G G
We have shown previously [McCool, Forstner and Forstner (1994) Biochem. J. 302, 111-118] using pulse-chase labelling of mucin with [3H]threonine that LS180 colonic tumour cells synthesize and secrete...
Synthesis and secretion of mucin by the human colonic tumour cell line LS180.
McCool, D J, Forstner, J F, Forstner, G G
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media...
Roberton, A M, Mantle, M, Fahim, R E, Specian, R D, Bennick, A, Kawagishi, S, ...
The existence of a discrete 'link' peptide in epithelial mucins has been debated for many years. There is evidence that at least some mucins contain a specific 'link' peptide (or glycopeptide) that...
Mantle, M, Forstner, G G, Forstner, J F
Previous studies have shown that human small-intestinal mucin consists of high-Mr glycoproteins and a smaller S-S-bonded protein of 118 kDa. The major antigenic determinants of the mucin were...
Characterization and localization of the putative 'link' component in rat small-intestinal mucin.
Fahim, R E, Specian, R D, Forstner, G G, Forstner, J F
Rat intestinal mucin is polymerized by a putative 'link' component of Mr 118,000 that can be released from the native mucin by thiol reduction [Fahim, Forstner & Forstner (1983) Biochem. J. 209,...
Fahim, R E, Forstner, G G, Forstner, J F
An investigation was undertaken to discover whether mucin purified from secretions in the lumen of rat small intestine differed in structure or composition from intracellular mucin purified from rat...
Antigenic and structural features of goblet-cell mucin of human small intestine.
Mantle, M, Forstner, G G, Forstner, J F
With the use of a newly developed solid-phase radioimmunoassay method, the major antigenic determinants of human small-intestinal goblet-cell mucin were investigated and related to the overall...
Heterogeneity of rat goblet-cell mucin before and after reduction.
Fahim, R E, Forstner, G G, Forstner, J F
Goblet-cell mucin of rat small intestine was purified from mucosal scrapings by using centrifugation, Sepharose 4B and Sepharose 2B chromatography. The mucin was applied in low concentrations (1...
Enhancement of the viscosity of mucin by serum albumin.
List, S J, Findlay, B P, Forstner, G G, Forstner, J F
The interaction of serum albumin with a model epithelial mucin from pig stomach was explored by rotary viscometry. During 30 min of incubation of human serum albumin(20mg/ml) and pig gastric mucin...
Khatri, I A, Forstner, G G, Forstner, J F
The present study reveals that partial proteolytic degradation of rat Muc 2 mucin can occur rapidly even in the presence of a battery of proteinase inhibitors. During the initial steps of...
McCool, D J, Okada, Y, Forstner, J F, Forstner, G G
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to...
Bell, S L, Xu, G, Forstner, J F
DNA constructs based on the 534-amino-acid C-terminus of rat mucin protein Muc2 (RMC), were transfected into COS cells and the resultant (35)S-labelled dimers and monomers were detected by SDS/PAGE...