Comparison of the NMR solution structure and the x-ray crystal structure of rat metallothionein-2.
Braun, W, Vasák, M, Robbins, A H, Stout, C D, Wagner, G, Kägi, J H, ...
Metallothioneins are small cysteine-rich proteins capable of binding heavy metal ions such as Zn2+ and Cd2+. They are ubiquitous tissue components in higher organisms, which tentatively have been...
Zinc transfer from transcription factor IIIA fingers to thionein clusters.
Zeng, J, Vallee, B L, Kägi, J H
The rapid induction of thionein (apometallothionein) by many endogenous stimuli such as steroid hormones, cytokines, and second messengers suggests that this cysteine-rich, metal binding protein...
Metal thiolate clusters in cobalt(II)-metallothionein.
Rabbit liver metallothionein-1 in which all seven metal-binding sites are occupied by cobalt(II) exhibits spectral features typical of tetrathiolate coordination with approximate Td microsymmetry...
Pfister, K, Kägi, J H, Christen, P
Catalysis-linked conformational transitions of aspartate aminotransferase (cytosolic isoenzyme from pig heart; L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1) have been probed by infrared...
Amino-acid sequence of equine renal metallothionein-1B.
Kojima, Y, Berger, C, Vallee, B L, Kägi, J H
The amino-acid sequence of a metallothionein is reported. Metallothionein is a widely distributed, extremely cysteine-rich, low-molecular-weight protein containing large amounts of cadmium and/or...
Cell- and inducer-specific accretion of human isometallothioneins.
Cavigelli, M, Kägi, J H, Hunziker, P E
The synthesis of metallothionein (MT) was investigated in three different human epithelial cell lines, each derived from one of the embryonic germ layers. The accretion of different isoforms of the...
Induction of metallothionein synthesis by cadmium and zinc in cultured rabbit kidney cells (RK-13).
Wan, M, Hunziker, P E, Kägi, J H
The effects of increasing concentrations of Zn(II) and Cd(II) on the expression of the four isometallothioneins (isoMTs), namely MT-1a, MT-2a, MT-2d and MT-2e, in rabbit kidney cells (RK-13) and the...
Isolation and characterization of six human hepatic isometallothioneins.
Human hepatic metallothionein (MT) was separated into six isoforms by using reversed-phase h.p.l.c. at the analytical and preparative levels. By comparison with the h.p.l.c. elution profiles of the...
Klauser, S, Kägi, J H, Wilson, K J
Reverse-phase high-pressure ('performance') liquid chromatography was used to characterize the isometallothioneins in preparations isolated from tissues of a variety of animals by 'conventional'...
Metallothionein accretion in human hepatic cells is linked to cellular proliferation.
Studer, R, Vogt, C P, Cavigelli, M, Hunziker, P E, Kägi, J H
The basal amounts of metallothionein (MT) and its rates of biosynthesis were compared in resting and proliferating Chang liver (CCl-13) cells. In resting cells the total amounts of the detectable...
Structure of mammalian metallothionein.
Kägi, J H, Vasák, M, Lerch, K, Gilg, D E, Hunziker, P, Bernhard, W R, ...
All mammalian metallothioneins characterized contain a single polypeptide chain of 61 amino acid residues, among them 20 cysteines providing the ligands for seven metal-binding sites. Native...
Comparison of the NMR solution structure and the x-ray crystal structure of rat metallothionein-2.
Braun, W, Vasák, M, Robbins, A H, Stout, C D, Wagner, G, Kägi, J H, ...
Metallothioneins are small cysteine-rich proteins capable of binding heavy metal ions such as Zn2+ and Cd2+. They are ubiquitous tissue components in higher organisms, which tentatively have been...
Zinc transfer from transcription factor IIIA fingers to thionein clusters.
Zeng, J, Vallee, B L, Kägi, J H
The rapid induction of thionein (apometallothionein) by many endogenous stimuli such as steroid hormones, cytokines, and second messengers suggests that this cysteine-rich, metal binding protein...
Metal thiolate clusters in cobalt(II)-metallothionein.
Rabbit liver metallothionein-1 in which all seven metal-binding sites are occupied by cobalt(II) exhibits spectral features typical of tetrathiolate coordination with approximate Td microsymmetry...
Pfister, K, Kägi, J H, Christen, P
Catalysis-linked conformational transitions of aspartate aminotransferase (cytosolic isoenzyme from pig heart; L-aspartate:2-oxoglutarate aminotransferase, EC 2.6.1.1) have been probed by infrared...
Amino-acid sequence of equine renal metallothionein-1B.
Kojima, Y, Berger, C, Vallee, B L, Kägi, J H
The amino-acid sequence of a metallothionein is reported. Metallothionein is a widely distributed, extremely cysteine-rich, low-molecular-weight protein containing large amounts of cadmium and/or...
Cell- and inducer-specific accretion of human isometallothioneins.
Cavigelli, M, Kägi, J H, Hunziker, P E
The synthesis of metallothionein (MT) was investigated in three different human epithelial cell lines, each derived from one of the embryonic germ layers. The accretion of different isoforms of the...
Induction of metallothionein synthesis by cadmium and zinc in cultured rabbit kidney cells (RK-13).
Wan, M, Hunziker, P E, Kägi, J H
The effects of increasing concentrations of Zn(II) and Cd(II) on the expression of the four isometallothioneins (isoMTs), namely MT-1a, MT-2a, MT-2d and MT-2e, in rabbit kidney cells (RK-13) and the...
Isolation and characterization of six human hepatic isometallothioneins.
Human hepatic metallothionein (MT) was separated into six isoforms by using reversed-phase h.p.l.c. at the analytical and preparative levels. By comparison with the h.p.l.c. elution profiles of the...
Klauser, S, Kägi, J H, Wilson, K J
Reverse-phase high-pressure ('performance') liquid chromatography was used to characterize the isometallothioneins in preparations isolated from tissues of a variety of animals by 'conventional'...
Metallothionein accretion in human hepatic cells is linked to cellular proliferation.
Studer, R, Vogt, C P, Cavigelli, M, Hunziker, P E, Kägi, J H
The basal amounts of metallothionein (MT) and its rates of biosynthesis were compared in resting and proliferating Chang liver (CCl-13) cells. In resting cells the total amounts of the detectable...
Structure of mammalian metallothionein.
Kägi, J H, Vasák, M, Lerch, K, Gilg, D E, Hunziker, P, Bernhard, W R, ...
All mammalian metallothioneins characterized contain a single polypeptide chain of 61 amino acid residues, among them 20 cysteines providing the ligands for seven metal-binding sites. Native...
Gehrig, P. M., You, C., Dallinger, R., Gruber, C., Brouwer, M., Kägi, J. H., ...
Electrospray ionization (ESI) mass spectra of both well-characterized and novel metallothioneins (MTs) from various species were recorded to explore their metal-ion-binding modes and stoichiometries....