J. Heberle

Publication List Details

Period

2000 - 2008

Number

53

Co-Authors

Chromophore composition of a heterologously expressed BLUF-domain. (2004)

Laan, W.W.J., Bednarz, T., Heberle, J., Hellingwerf, K.J.

Upon heterologous expression of the BLUF (for: Blue-Light sensing Using Flavin) domain from AppA, a transcriptional anti-repressor from Rhodobacter sphaeroides, in Escherichia coli, photoactive...

A local area network of protonated water molecules (2004)

Heberle, J.

This version is available at the following Publisher URL: http://www.biophysj.org/

The Protein Tethered Lipid Bilayer - a novel mimic of the biological membrane (2004)

Gieß, F., Friedrich, M., Heberle, J., Naumann, R., Knoll, W.

This version is available at the following Publisher URL: http://www.biophysj.org/

Proton translocation by bacteriorhodopsin in the absence of substantial conformational changes (2002)

Tittor,J., Paula,S., Subramaniam,S., Heberle,J., Henderson,R., Oesterhelt,D.

Unlike wild-type bacteriorhodopsin (BR), the BR triple mutant D96G/F171C/F219L has been shown to undergo only minor structural rearrangements during its photocycle. Nonetheless, the mutant is capable...

Proton translocation by bacteriorhodopsin in the absence of substantial conformational changes (2002)

Tittor, J., Paula, S., Subramaniam, S., Heberle, J., Henderson, R., Oesterhelt, D.

Unlike wild-type bacteriorhodopsin (BR), the BR triple mutant D96G/F171C/F219L has been shown to undergo only minor structural rearrangements during its photocycle. Nonetheless, the mutant is capable...

Assembly of τ protein into Alzheimer paired helical filaments depends on a local sequence motif (306VQIVYK311) forming β structure

Von Bergen, M., Friedhoff, P., Biernat, J., Heberle, J., Mandelkow, E.

We have searched for a minimal interaction motif in τ protein that supports the aggregation into Alzheimer-like paired helical filaments. Digestion of the repeat domain with different proteases...

Photoactive mitochondria: in vivo transfer of a light-driven proton pump into the inner mitochondrial membrane of Schizosaccharomyces pombe.

Hoffmann, A, Hildebrandt, V, Heberle, J, Büldt, G

The light-driven proton pump bacteriorhodopsin (bR) from Halobacterium salinarium has been genetically transferred into the inner mitochondrial membrane (IM) of the eukaryotic cell...

Bacteriorhodopsin expressed in Schizosaccharomyces pombe pumps protons through the plasma membrane.

Hildebrandt, V, Fendler, K, Heberle, J, Hoffmann, A, Bamberg, E, Büldt, G

Bacterioopsin (bO) from Halobacterium salinarium ("Halobacterium halobium") has been functionally expressed in a heterologous system, the fission yeast Schizosaccharomyces pombe. Regeneration of bO...

Surface-bound optical probes monitor protein translocation and surface potential changes during the bacteriorhodopsin photocycle.

Heberle, J, Dencher, N A

Light-induced H+ release and reuptake as well as surface potential changes inherent in the bacterio-rhodopsin reaction cycle were measured between 10 degrees C and 50 degrees C. Signals of optical pH...

Decoupling of photo- and proton cycle in the Asp85-->Glu mutant of bacteriorhodopsin.

Heberle, J, Oesterhelt, D, Dencher, N A

Surface bound pH indicators were applied to study the proton transfer reactions in the mutant Asp85-->Glu of bacteriorhodopsin in the native membrane. The amino acid replacement induces a drastic...

Structural equilibrium fluctuations in mesophilic and thermophilic alpha-amylase.

Fitter, J, Heberle, J

By comparing a mesophilic alpha-amylase with its thermophilic homolog, we investigated the relationship between thermal stability and internal equilibrium fluctuations. Fourier transform infrared...

Vibrational Spectroscopy of an Algal Phot-LOV1 Domain Probes the Molecular Changes Associated with Blue-Light Reception

Ataka, K., Hegemann, P., Heberle, J.

The LOV1 domain of the blue light Phot1-receptor (phototropin homolog) from Chlamydomonas reinhardtii has been studied by vibrational spectroscopy. The FMN modes of the dark state of LOV1 were...

Assembly of τ protein into Alzheimer paired helical filaments depends on a local sequence motif (306VQIVYK311) forming β structure

Von Bergen, M., Friedhoff, P., Biernat, J., Heberle, J., Mandelkow, E.

We have searched for a minimal interaction motif in τ protein that supports the aggregation into Alzheimer-like paired helical filaments. Digestion of the repeat domain with different proteases...

Photoactive mitochondria: in vivo transfer of a light-driven proton pump into the inner mitochondrial membrane of Schizosaccharomyces pombe.

Hoffmann, A, Hildebrandt, V, Heberle, J, Büldt, G

The light-driven proton pump bacteriorhodopsin (bR) from Halobacterium salinarium has been genetically transferred into the inner mitochondrial membrane (IM) of the eukaryotic cell...

Bacteriorhodopsin expressed in Schizosaccharomyces pombe pumps protons through the plasma membrane.

Hildebrandt, V, Fendler, K, Heberle, J, Hoffmann, A, Bamberg, E, Büldt, G

Bacterioopsin (bO) from Halobacterium salinarium ("Halobacterium halobium") has been functionally expressed in a heterologous system, the fission yeast Schizosaccharomyces pombe. Regeneration of bO...

Surface-bound optical probes monitor protein translocation and surface potential changes during the bacteriorhodopsin photocycle.

Heberle, J, Dencher, N A

Light-induced H+ release and reuptake as well as surface potential changes inherent in the bacterio-rhodopsin reaction cycle were measured between 10 degrees C and 50 degrees C. Signals of optical pH...

Decoupling of photo- and proton cycle in the Asp85-->Glu mutant of bacteriorhodopsin.

Heberle, J, Oesterhelt, D, Dencher, N A

Surface bound pH indicators were applied to study the proton transfer reactions in the mutant Asp85-->Glu of bacteriorhodopsin in the native membrane. The amino acid replacement induces a drastic...

Structural equilibrium fluctuations in mesophilic and thermophilic alpha-amylase.

Fitter, J, Heberle, J

By comparing a mesophilic alpha-amylase with its thermophilic homolog, we investigated the relationship between thermal stability and internal equilibrium fluctuations. Fourier transform infrared...

Vibrational Spectroscopy of an Algal Phot-LOV1 Domain Probes the Molecular Changes Associated with Blue-Light Reception

Ataka, K., Hegemann, P., Heberle, J.

The LOV1 domain of the blue light Phot1-receptor (phototropin homolog) from Chlamydomonas reinhardtii has been studied by vibrational spectroscopy. The FMN modes of the dark state of LOV1 were...

Time-Resolved Microspectroscopy on a Single Crystal of Bacteriorhodopsin Reveals Lattice-Induced Differences in the Photocycle Kinetics

Efremov, R., Gordeliy, V. I., Heberle, J., Büldt, G.

The determination of the intermediate state structures of the bacteriorhodopsin photocycle has lead to an unprecedented level of understanding of the catalytic process exerted by a membrane protein....

Binding of Ca2+ to Glutamic Acid-Rich Polypeptides from the Rod Outer Segment

Haber-Pohlmeier, S., Abarca-Heidemann, K., Körschen, H. G., Dhiman, H. Kaur, Heberle, J., Schwalbe, H., ...

Rod photoreceptors contain three different glutamic acid-rich proteins (GARPs) that have been proposed to control the propagation of Ca2+ from the site of its entry at the cyclic nucleotide-gated...

Resolving voltage-dependent structural changes of a membrane photoreceptor by surface-enhanced IR difference spectroscopy

Jiang, X., Zaitseva, E., Schmidt, M., Siebert, F., Engelhard, M., Schlesinger, R., ...

Membrane proteins are molecular machines that transport ions, solutes, or information across the cell membrane. Electrophysiological techniques have unraveled many functional aspects of ion channels...