Attridge, Stephen Richard, Wallerstrom, Gun, Li, B. L., Morona, Renato, Holmgren, J.
Copyright © 2009 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V
Attridge, Stephen Richard, Wallerstrom, Gun, Li, B. L., Morona, Renato, Holmgren, J.
Copyright © 2009 Elsevier B.V. All rights reserved. ScienceDirect® is a registered trademark of Elsevier B.V
Vult Von Steyern, Per, Haag, P, Nilner, Krister, Ebbesson, S, Holmgren, J
The aim of the present study was to investigate the fracture resistance of zirconia crowns and to compare the results with crowns made of a material with known clinical performance (alumina) in away...
Clemens, J., Rao, M., Sack, D., Ahmed, F., Khan, M.R., Chakraborty, J., ...
In a field trial carried out in 1985 in Matlab, Bangladesh, the authors evaluated whether subjects who developed Vibrio cholerae 01 infections during the first year after earlier receipt of B...
Clemens, J., Rao, M., Sack, D., Ahmed, F., Khan, M. R., Chakraborty, J., ...
In a field trial carried out in 1985 in Matlab, Bangladesh, the authors evaluated whether subjects who developed Vibrio cholerae 01 infections during the first year after earlier receipt of B...
Field trial of oral cholera vaccines in Bangladesh (1988)
Clemens, J.D., Harris, J.R., Sack, D.A., Chakraborty, J., Ahmed, F., Stanton, B.F., ...
The possibility that antibody responses in serum, saliva, or breast milk samples to oral vaccines or enteric infections may reflect the intestinal immune response was evaluated in Bangladeshi...
Cellulose GMI binding of cholera toxin in family contracts of cholera patients (1982)
Hossain, M.M., Glass, Roger, Holmgren, J., Greenough, W.B., Stoll, B.J., Khan, M.U., ...
Rabbani, G.H., Greenough III, W.B., Holmgren, J., Kirkwood, B.
A randomised controlled trial was conducted to investigate the ability of chlorpromazine to reduce intestinal secretion in cholera. Chlorpromazine had reduced loss of intestinal fluid in animals with...
Intestinal antibody responses after immunisation with cholera B subunit (1982)
Svennerholm, A.M., Sack, D.A., Holmgren, J., Bardhan, P.K.
In about 80% of Bangladeshi volunteers a single oral or intramuscular immunisation with a new cholera toxoid immunogen (B subunit) gave rise to a local intestinal secretory immunoglobulin A (IgA)...
Chlorpromazine reduces fluid-loss in cholera (1979)
Rabbani, G.H., Greenough, William B. III, Holmgren, J., Lönnroth, I.
Because chlorpromazine inhibited cholera-toxin-stimulated intestinal adenylate cyclase and fluid secretion in laboratory animals its ability to reduce fluid-loss in human cholera was investigated....
Sun, J B, Rask, C, Olsson, T, Holmgren, J, Czerkinsky, C
Oral administration of autoantigens can prevent and partially suppress autoimmune diseases in a number of experimental models, Depending on the dose of antigen fed, this approach appears to involve...
Sun, J B, Holmgren, J, Czerkinsky, C
Oral administration of antigens, including allergens and autoantigens, may be an efficient way to prevent diseases associated with untoward immune responses to self- and non-self-antigens. However,...
Furuta, Y, Eriksson, K, Svennerholm, B, Fredman, P, Horal, P, Jeansson, S, ...
The rectal and genital tract mucosae are considered to be major sites of entry for the human immunodeficiency virus (HIV) during sexual contact. We now demonstrate that vaginal epithelial cells can...
Vahlne, A, Horal, P, Eriksson, K, Jeansson, S, Rymo, L, Hedström, K G, ...
Site-directed immunization was employed to identify sites on the envelope glycoprotein gp120 for antibody-mediated neutralization of human immunodeficiency virus type 1 (HIV-1). Antisera were raised...
Therapeutic vaccination is an attractive strategy to control infection and disease caused by Helicobacter pylori. In mice infected with H. pylori we have studied the protective effect of oral...
Raghavan, S., Hjulström, M., Holmgren, J.
The protective effect of therapeutic oral immunization with homologous and heterologous formalin-inactivated Helicobacter pylori cells given together with cholera toxin as an adjuvant was evaluated...
Induction of compartmentalized B-cell responses in human tonsils.
Quiding-Järbrink, M, Granström, G, Nordström, I, Holmgren, J, Czerkinsky, C
The capacity of tonsillar and nasal mucosal lymphoid tissues to serve as induction sites of local and/or distant B-cell responses in humans has been examined. The frequencies of vaccine-specific...
Local and systemic antibody responses to dextran-cholera toxin B subunit conjugates.
Bergquist, C, Lagergård, T, Lindblad, M, Holmgren, J
This study was designed to test local and systemic immunity following mucosal immunization with a polysaccharide-protein conjugate. After preparing and characterizing dextran-cholera toxin B subunit...
Lebens, M, Shahabi, V, Bäckström, M, Houze, T, Lindblad, N, Holmgren, J
Three variants of the cholera toxin B subunit (CTB) were generated by site-specific mutagenesis in which regions of the mature protein were altered to the composition found at the corresponding...
Attridge, S R, Manning, P A, Holmgren, J, Jonson, G
A previously described in-frame deletion in mshA--the gene encoding the structural subunit of the mannose-sensitive hemagglutinin pilus--has been introduced into the chromosome of three El Tor O1...
Jonson, G, Osek, J, Svennerholm, A M, Holmgren, J
We have characterized 11 isolates of Vibrio cholerae O139 Bengal with regard to properties deemed to be relevant for development of a vaccine against O139 cholera. For most strains two colony...
Bergquist, C, Lagergård, T, Holmgren, J
We have conjugated cholera toxin (CT) B subunit (CTB) to dextran and studied the effect in mice of previous immunization with CT and CTB on the response to dextran after intranasal immunizations with...
Bergquist, C, Johansson, E L, Lagergård, T, Holmgren, J, Rudin, A
Forty-five volunteers were vaccinated twice intranasally with 10, 100, or 1,000 microg of cholera toxin B subunit (CTB). Blood and nasal and vaginal secretions were collected before and 1 week after...
Cholera toxin and heat-labile enterotoxin (LT) are structurally similar oligomeric proteins which are capable of being efficiently secreted from Vibrio cholerae. Here we report that these proteins...
Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli.
Palva, E T, Hirst, T R, Hardy, S J, Holmgren, J, Randall, L
Escherichia coli K-12 minicells were employed to investigate the biosynthesis of plasmid-encoded, heat-labile enterotoxin of E. coli. Two polypeptide species related to the B subunit of the toxin...
Jonson, G, Holmgren, J, Svennerholm, A M
The expression of toxin-coregulated pili (TCP) and their structural subunit TcpA was compared in 20 strains of Vibrio cholerae of the classical and El Tor biotypes. Bacteria were isolated from the...
Osek, J, Svennerholm, A M, Holmgren, J
Both specific polyclonal antiserum and monoclonal antibodies against mannose-binding hemagglutinin fimbriae of Vibrio cholerae (mannose-sensitive hemagglutinin [MSHA]) were shown to protect against...
Czerkinsky, C, Svennerholm, A M, Quiding, M, Jonsson, R, Holmgren, J
We examined whether immunization with a newly developed oral cholera vaccine would elicit gut-derived antibody-producing cells in the blood and in distant mucosal tissues, such as the minor salivary...
Svennerholm, A M, Wennerås, C, Holmgren, J, McConnell, M M, Rowe, B
The roles of the subcomponents of colonization factor antigen II, the coli surface antigens CS1, CS2, and CS3, as colonization factors and protective antigens was studied in a nonligated rabbit...
Hess, C B, Niesel, D W, Holmgren, J, Jonson, G, Klimpel, G R
The role of bacterial invasion and subsequent intracellular metabolism or replication, or both, in the induction of interferon (IFN) production in primary cultures of murine embryo fibroblasts (MEFs)...
Holmgren, J, Svennerholm, A M, Gothefors, L, Jertborn, M, Stoll, B
We examined whether infection with enterotoxigenic Escherichia coli (ETEC) producing the heat-labile enterotoxin (LT) can prime the gut immune system to respond more efficiently to the...
Svennerholm, A M, Vidal, Y L, Holmgren, J, McConnell, M M, Rowe, B
The role of the PCF8775 antigen and its antigenic subcomponents, in particular, the coli surface (CS) antigen CS6, as colonization factors and protective antigens was studied in the reversible...
Fusion of genes encoding Escherichia coli heat-stable enterotoxin and outer membrane protein OmpC.
Saarilahti, H T, Palva, E T, Holmgren, J, Sanchez, J
The OmpC outer membrane protein of Escherichia coli was used as a carrier molecule for the nonimmunogenic heat-stable enterotoxin STa. Two fragments of different lengths of the gene encoding STa were...
Black, R E, Levine, M M, Clements, M L, Young, C R, Svennerholm, A M, Holmgren, J
Natural protection from cholera is associated with local intestinal antibacterial and antitoxic antibodies, which appear to act synergistically. Although current parenteral cholera vaccines offer...
Ljungström, I, Holmgren, J, Svennerholm, A M, Ferrante, A
The effect of Giardia muris infection on the diarrheal response and gut mucosal antibody response to cholera toxin was examined in mice. The results obtained showed that the fluid accumulation in...
Svennerholm, A M, Levine, M M, Holmgren, J
A soluble hemagglutinin/protease from Vibrio cholerae has been suggested to be a putative virulence factor and protective antigen. However, clinical cholera infection gave rise to detectable serum...
Svennerholm, A M, Strömberg, G J, Holmgren, J
Soluble hemagglutinin (HA) from an El Tor Vibrio cholerae strain (serotype Ogawa) was purified by means of a sequence of salt precipitation, gel filtration, and agarose electrophoresis. The purified...
Lindholm, L, Holmgren, J, Wikström, M, Karlsson, U, Andersson, K, Lycke, N
Seventy monoclonal antibodies to cholera toxin were prepared and characterized. All were of immunoglobulin G (IgG) isotypes (39 IgG1, 29 IgG2, and 2 IgG3). A total of 61 clones produced antibody...
Sanchez, J, Holmgren, J, Svennerholm, A M
A recombinant gene fusion protein composed of an Escherichia coli heat-stable enterotoxin (STa) peptide epitope fused to the amino end of the cholera toxin B subunit was used to detect STa produced...
Jertborn, M, Svennerholm, A M, Holmgren, J
The possibility that antibody responses in serum, saliva, or breast milk samples to oral vaccines or enteric infections may reflect the intestinal immune response was evaluated in Bangladeshi...
Svennerholm, A M, Wikström, M, Lindblad, M, Holmgren, J
Seven monoclonal antibodies (MAbs) against heat-stable enterotoxin (ST) from a human Escherichia coli isolate were prepared and evaluated for their usefulness in an ST immunodetection assay, the ST...
Non-O1 Vibrio cholerae in Thailand: homology with cloned cholera toxin genes.
Hanchalay, S, Seriwatana, J, Echeverria, P, Holmgren, J, Tirapat, C, Moseley, S L, ...
We examined 281 non-O1 Vibrio cholerae isolates from Thailand for homology with genes coding for cholera toxin. Five isolates from environmental sources were homologous with the cholera toxin gene...
Bäck, E, Svennerholm, A M, Holmgren, J, Möllby, R
The GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA), an immunological method for detection of Escherichia coli heat-labile enterotoxin (LT), was quantitatively and qualitatively...
Wiedermann, U, Hanson, L A, Holmgren, J, Kahu, H, Dahlgren, U I
To investigate the importance of vitamin A in the ability to respond to oral antigen administration, rats were fed a vitamin A-free diet. The animals were immunized perorally three times with a...
Hardy, S J, Holmgren, J, Johansson, S, Sanchez, J, Hirst, T R
In this paper we study the assembly, in vivo and in vitro, of a family of hexameric, heat-labile enterotoxins produced by diarrheagenic bacteria. The toxins, which consist of an A subunit and five B...
We have constructed an overexpression system in which the gene encoding the B subunit of cholera toxin (CTB) was placed under the control of the strong tacP promoter in a wide host range plasmid....
Quiding, M, Nordström, I, Kilander, A, Andersson, G, Hanson, L A, Holmgren, J, ...
We have examined secretory antibody and cell-mediated immune responses to oral cholera vaccine in the human gastrointestinal mucosa. Freshly isolated peripheral blood lymphocytes and intestinal...
The secretion of enterotoxin by Vibrio cholerae is punctuated by the transient entry of the toxin subunits into the periplasm. In this paper, we show that the subunits oligomerize into an assembled...
Osek, J, Jonson, G, Svennerholm, A M, Holmgren, J
Vibrio cholerae O1, which exists as two biotypes, classical and El Tor, expresses fimbrial antigens called toxin-coregulated pili (TCP) and mannose-sensitive hemagglutinin (MSHA) pili, respectively....
The maltose regulon of Vibrio cholerae affects production and secretion of virulence factors.
Lång, H, Jonson, G, Holmgren, J, Palva, E T
The effects of maltose on production and secretion of virulence factors of Vibrio cholerae in strain X28214, classical biotype, and in maltose-defective transposon mutants constructed from this...
Raghavan, S., Nyström, J., Fredriksson, M., Holmgren, J., Harandi, A. M.
Bacterial DNA and unmethylated CpG oligodeoxynucleotides (CpG ODN) are known to be potent stimulators of the innate immune system in vitro and in vivo. We therefore investigated if oral...
Czerkinsky, C, Russell, M W, Lycke, N, Lindblad, M, Holmgren, J
Generation of local and systemic immune responses by the oral administration of antigens is frequently inefficient, requiring large quantities of immunogens and yielding only modest antibody...
A protocol was elaborated for the adoptive transfer of lymphocytes from mice which were orally immunized with cholera toxin (CT) to enable the study of long-term gut mucosal immunological memory at...
Jonson, G, Svennerholm, A M, Holmgren, J
Vibrio cholerae O1 bacteria harvested directly from ligated or nonligated intestines of rabbits with experimental cholera expressed at least 7 to 8 novel, in vivo-specific cell envelope (env)...
Holmgren, J, Fredman, P, Lindblad, M, Svennerholm, A M, Svennerholm, L
The receptors for cholera toxin and Escherichia coli heat-labile toxin (LT) in rabbit small intestinal epithelium were characterized and compared. (i) In vivo studies in ligated intestinal loops...
Holmgren, J, Svennerholm, A M, Lindblad, M
The two biotypes of Vibrio cholerae were found to have cell-associated hemagglutinins which differ with regard to binding to different species of erythrocytes and inhibition by monosaccharides. A...
Holmgren, J, Svennerholm, L, Elwing, H, Fredman, P, Strannegård, O
The binding of Sendai virus to polystyrene petri dishes to which various gangliosides of defined structures had been adsorbed was determined. The ganglioside-bound virus was visualized either by a...
Holmgren, J, Svennerholm, A M, Ahrén, C
Human milk and colostrum samples were divided into an immunoglobulin and a nonimmunoglobulin fraction by immunosorbent chromatography. The ability of these fractions to inhibit bacterial cell...
Hirst, T R, Sanchez, J, Kaper, J B, Hardy, S J, Holmgren, J
A genetically engineered Vibrio cholerae strain from which the cholera toxin genes had previously been deleted was used as a host in which to study the expression and secretion of related toxins and...
Holmgren, J, Svennerholm, A M, Ouchterlony, O, Anderson, A, Walletström, G, Westerberg-Berndtsson, U
The protective effect of enternal and parenteral immunization with cholera toxin antigen against experimental cholera in rabbits was studied by using the small-bowel loop technique. Subcutaneous...
Holmgren, J., Lönnroth, I., Ouchterlony, Ö.
Two crude toxin preparations of Vibrio cholerae, labeled lot 4493 G (Inaba) and lot 001 (Ogawa), consisting of freeze-dried culture filtrate, were studied with regard to toxicity,...
Subcutaneous immunization of rabbits with a combination of Vibrio cholerae lipopolysaccharide (LPS) and enterotoxin induced a more than 100-fold-higher degree of protection against intestinal...
An in vitro culture technique with synthesis of 14C-labeled protein has been used to study immunoglobulin and specific-antibody formation by spleen, mesenteric lymph nodes, Peyer's patches, and small...
Svennerholm, A, Lange, S, Holmgren, J
The importance of locally and systemically formed antibodies of various classes for protection against experimental cholera has been studied in mice immunized with cholera toxin. Groups of mice were...
Holmgren, J., Andersson, Å., Wallerstrom, G., Ouchterlony, Ö.
By use of the ileal loop technique, the resistance to challenge with cholera enterotoxin was compared between unimmunized rabbits and rabbits immunized with toxin or toxoids. It was shown that...
Enzyme-Linked Immunosorbent Assays for Cholera Serology
The enzyme-linked immunosorbent assay (ELISA) principle of Engvall and Perlmann, which employs antigen-coated tubes and enzyme-labeled anti-immunoglobulins, was elaborated for use in cholera...
Holmgren, J., Lönnroth, I., Svennerholm, L.
By a double-diffusion precipitation-in-gel technique, isolated cholera toxin as well as its natural toxoid were shown to be fixed and precipitated by the ganglioside GM1 but not by any of the related...
Local Antibody Production in Experimental Pyelonephritis: Amount, Avidity, and Immunoglobulin Class
Smith, J., Holmgren, J., Ahlstedt, S., Hanson, L. A.
Local antibody formation in infected rabbit kidneys was studied with three techniques: the ammonium sulfate precipitation technique, the enzyme-linked immunosorbent assay, and by binding of newly...
Ultrastructural localization of cell membrane GM1 ganglioside by cholera toxin
An immunoelectron microscopic method is described for sensitive high-resolution visualization of tissuebound cholera toxin. The principle is to incubate cells or tissue sections with toxin and then...
Interaction of cholera toxin and membrane GM1 ganglioside of small intestine.
Holmgren, J, Lönnroth, I, Månsson, J, Svennerholm, L
Ganglioside GM1 was isolated from the small intestinal mucosa of man, pig, and beef and amounted to 0.1, 2.0, and 43 nmol per g fresh weight, respectively. These differences in GM1 content were...
Quiding-Järbrink, M, Nordström, I, Granström, G, Kilander, A, Jertborn, M, Butcher, E C, ...
Expression of the adhesion molecules CD44, L-selectin (CD62L), and integrin alpha 4 beta 7 by antibody-secreting cells (ASC) was examined in human volunteers after oral, rectal, intranasal, or...
Svennerholm, A M, Lange, S, Holmgren, J
The influence in immunization with cholera toxin of the route and antigen dose on intestinal antibody formation and protective immunity against experimental cholera was studied in mice....
Svennerholm, A M, Hanson, L A, Holmgren, J, Lindblad, B S, Nilsson, B, Quereshi, F
The capacity of subcutaneous cholera vaccination to induce an antibody response in milk and saliva was studied in lactating Swedish and Pakistani women, since secretory immunoglobulin A (SIgA)...
Detection by monoclonal antibody of carbohydrate antigen CA 50 in serum of patients with carcinoma.
Holmgren, J, Lindholm, L, Persson, B, Lagergård, T, Nilsson, O, Svennerholm, L, ...
A solid phase radioimmunoassay was devised for measuring the value of the carcinoma associated carbohydrate antigen CA 50 in serum based on the use of a specific monoclonal antibody (C 50). Samples...
Rabbani, G H, Greenough, W B, Holmgren, J, Kirkwood, B
A randomised controlled trial was conducted to investigate the ability of chlorpromazine to reduce intestinal secretion in cholera. Chlorpromazine had reduced loss of intestinal fluid in animals with...
Letter: New enterotoxinogenic bacteria isolated.
Wadström, T, Kettis, A A, Habte, D, Holmgren, J, Meeuwisse, G, Möllby, R, ...
Sun, J B, Rask, C, Olsson, T, Holmgren, J, Czerkinsky, C
Oral administration of autoantigens can prevent and partially suppress autoimmune diseases in a number of experimental models, Depending on the dose of antigen fed, this approach appears to involve...
Sun, J B, Holmgren, J, Czerkinsky, C
Oral administration of antigens, including allergens and autoantigens, may be an efficient way to prevent diseases associated with untoward immune responses to self- and non-self-antigens. However,...
Furuta, Y, Eriksson, K, Svennerholm, B, Fredman, P, Horal, P, Jeansson, S, ...
The rectal and genital tract mucosae are considered to be major sites of entry for the human immunodeficiency virus (HIV) during sexual contact. We now demonstrate that vaginal epithelial cells can...
Vahlne, A, Horal, P, Eriksson, K, Jeansson, S, Rymo, L, Hedström, K G, ...
Site-directed immunization was employed to identify sites on the envelope glycoprotein gp120 for antibody-mediated neutralization of human immunodeficiency virus type 1 (HIV-1). Antisera were raised...
Therapeutic vaccination is an attractive strategy to control infection and disease caused by Helicobacter pylori. In mice infected with H. pylori we have studied the protective effect of oral...
Raghavan, S., Hjulström, M., Holmgren, J.
The protective effect of therapeutic oral immunization with homologous and heterologous formalin-inactivated Helicobacter pylori cells given together with cholera toxin as an adjuvant was evaluated...
Induction of compartmentalized B-cell responses in human tonsils.
Quiding-Järbrink, M, Granström, G, Nordström, I, Holmgren, J, Czerkinsky, C
The capacity of tonsillar and nasal mucosal lymphoid tissues to serve as induction sites of local and/or distant B-cell responses in humans has been examined. The frequencies of vaccine-specific...
Local and systemic antibody responses to dextran-cholera toxin B subunit conjugates.
Bergquist, C, Lagergård, T, Lindblad, M, Holmgren, J
This study was designed to test local and systemic immunity following mucosal immunization with a polysaccharide-protein conjugate. After preparing and characterizing dextran-cholera toxin B subunit...
Lebens, M, Shahabi, V, Bäckström, M, Houze, T, Lindblad, N, Holmgren, J
Three variants of the cholera toxin B subunit (CTB) were generated by site-specific mutagenesis in which regions of the mature protein were altered to the composition found at the corresponding...
Attridge, S R, Manning, P A, Holmgren, J, Jonson, G
A previously described in-frame deletion in mshA--the gene encoding the structural subunit of the mannose-sensitive hemagglutinin pilus--has been introduced into the chromosome of three El Tor O1...
Jonson, G, Osek, J, Svennerholm, A M, Holmgren, J
We have characterized 11 isolates of Vibrio cholerae O139 Bengal with regard to properties deemed to be relevant for development of a vaccine against O139 cholera. For most strains two colony...
Bergquist, C, Lagergård, T, Holmgren, J
We have conjugated cholera toxin (CT) B subunit (CTB) to dextran and studied the effect in mice of previous immunization with CT and CTB on the response to dextran after intranasal immunizations with...
Bergquist, C, Johansson, E L, Lagergård, T, Holmgren, J, Rudin, A
Forty-five volunteers were vaccinated twice intranasally with 10, 100, or 1,000 microg of cholera toxin B subunit (CTB). Blood and nasal and vaginal secretions were collected before and 1 week after...
Cholera toxin and heat-labile enterotoxin (LT) are structurally similar oligomeric proteins which are capable of being efficiently secreted from Vibrio cholerae. Here we report that these proteins...
Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli.
Palva, E T, Hirst, T R, Hardy, S J, Holmgren, J, Randall, L
Escherichia coli K-12 minicells were employed to investigate the biosynthesis of plasmid-encoded, heat-labile enterotoxin of E. coli. Two polypeptide species related to the B subunit of the toxin...
Jonson, G, Holmgren, J, Svennerholm, A M
The expression of toxin-coregulated pili (TCP) and their structural subunit TcpA was compared in 20 strains of Vibrio cholerae of the classical and El Tor biotypes. Bacteria were isolated from the...
Osek, J, Svennerholm, A M, Holmgren, J
Both specific polyclonal antiserum and monoclonal antibodies against mannose-binding hemagglutinin fimbriae of Vibrio cholerae (mannose-sensitive hemagglutinin [MSHA]) were shown to protect against...
Czerkinsky, C, Svennerholm, A M, Quiding, M, Jonsson, R, Holmgren, J
We examined whether immunization with a newly developed oral cholera vaccine would elicit gut-derived antibody-producing cells in the blood and in distant mucosal tissues, such as the minor salivary...
Svennerholm, A M, Wennerås, C, Holmgren, J, McConnell, M M, Rowe, B
The roles of the subcomponents of colonization factor antigen II, the coli surface antigens CS1, CS2, and CS3, as colonization factors and protective antigens was studied in a nonligated rabbit...
Hess, C B, Niesel, D W, Holmgren, J, Jonson, G, Klimpel, G R
The role of bacterial invasion and subsequent intracellular metabolism or replication, or both, in the induction of interferon (IFN) production in primary cultures of murine embryo fibroblasts (MEFs)...
Holmgren, J, Svennerholm, A M, Gothefors, L, Jertborn, M, Stoll, B
We examined whether infection with enterotoxigenic Escherichia coli (ETEC) producing the heat-labile enterotoxin (LT) can prime the gut immune system to respond more efficiently to the...
Svennerholm, A M, Vidal, Y L, Holmgren, J, McConnell, M M, Rowe, B
The role of the PCF8775 antigen and its antigenic subcomponents, in particular, the coli surface (CS) antigen CS6, as colonization factors and protective antigens was studied in the reversible...
Fusion of genes encoding Escherichia coli heat-stable enterotoxin and outer membrane protein OmpC.
Saarilahti, H T, Palva, E T, Holmgren, J, Sanchez, J
The OmpC outer membrane protein of Escherichia coli was used as a carrier molecule for the nonimmunogenic heat-stable enterotoxin STa. Two fragments of different lengths of the gene encoding STa were...
Black, R E, Levine, M M, Clements, M L, Young, C R, Svennerholm, A M, Holmgren, J
Natural protection from cholera is associated with local intestinal antibacterial and antitoxic antibodies, which appear to act synergistically. Although current parenteral cholera vaccines offer...
Ljungström, I, Holmgren, J, Svennerholm, A M, Ferrante, A
The effect of Giardia muris infection on the diarrheal response and gut mucosal antibody response to cholera toxin was examined in mice. The results obtained showed that the fluid accumulation in...
Svennerholm, A M, Levine, M M, Holmgren, J
A soluble hemagglutinin/protease from Vibrio cholerae has been suggested to be a putative virulence factor and protective antigen. However, clinical cholera infection gave rise to detectable serum...
Svennerholm, A M, Strömberg, G J, Holmgren, J
Soluble hemagglutinin (HA) from an El Tor Vibrio cholerae strain (serotype Ogawa) was purified by means of a sequence of salt precipitation, gel filtration, and agarose electrophoresis. The purified...
Lindholm, L, Holmgren, J, Wikström, M, Karlsson, U, Andersson, K, Lycke, N
Seventy monoclonal antibodies to cholera toxin were prepared and characterized. All were of immunoglobulin G (IgG) isotypes (39 IgG1, 29 IgG2, and 2 IgG3). A total of 61 clones produced antibody...
Sanchez, J, Holmgren, J, Svennerholm, A M
A recombinant gene fusion protein composed of an Escherichia coli heat-stable enterotoxin (STa) peptide epitope fused to the amino end of the cholera toxin B subunit was used to detect STa produced...
Jertborn, M, Svennerholm, A M, Holmgren, J
The possibility that antibody responses in serum, saliva, or breast milk samples to oral vaccines or enteric infections may reflect the intestinal immune response was evaluated in Bangladeshi...
Svennerholm, A M, Wikström, M, Lindblad, M, Holmgren, J
Seven monoclonal antibodies (MAbs) against heat-stable enterotoxin (ST) from a human Escherichia coli isolate were prepared and evaluated for their usefulness in an ST immunodetection assay, the ST...
Non-O1 Vibrio cholerae in Thailand: homology with cloned cholera toxin genes.
Hanchalay, S, Seriwatana, J, Echeverria, P, Holmgren, J, Tirapat, C, Moseley, S L, ...
We examined 281 non-O1 Vibrio cholerae isolates from Thailand for homology with genes coding for cholera toxin. Five isolates from environmental sources were homologous with the cholera toxin gene...
Bäck, E, Svennerholm, A M, Holmgren, J, Möllby, R
The GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA), an immunological method for detection of Escherichia coli heat-labile enterotoxin (LT), was quantitatively and qualitatively...
Wiedermann, U, Hanson, L A, Holmgren, J, Kahu, H, Dahlgren, U I
To investigate the importance of vitamin A in the ability to respond to oral antigen administration, rats were fed a vitamin A-free diet. The animals were immunized perorally three times with a...
Hardy, S J, Holmgren, J, Johansson, S, Sanchez, J, Hirst, T R
In this paper we study the assembly, in vivo and in vitro, of a family of hexameric, heat-labile enterotoxins produced by diarrheagenic bacteria. The toxins, which consist of an A subunit and five B...
We have constructed an overexpression system in which the gene encoding the B subunit of cholera toxin (CTB) was placed under the control of the strong tacP promoter in a wide host range plasmid....
Quiding, M, Nordström, I, Kilander, A, Andersson, G, Hanson, L A, Holmgren, J, ...
We have examined secretory antibody and cell-mediated immune responses to oral cholera vaccine in the human gastrointestinal mucosa. Freshly isolated peripheral blood lymphocytes and intestinal...
The secretion of enterotoxin by Vibrio cholerae is punctuated by the transient entry of the toxin subunits into the periplasm. In this paper, we show that the subunits oligomerize into an assembled...
Osek, J, Jonson, G, Svennerholm, A M, Holmgren, J
Vibrio cholerae O1, which exists as two biotypes, classical and El Tor, expresses fimbrial antigens called toxin-coregulated pili (TCP) and mannose-sensitive hemagglutinin (MSHA) pili, respectively....
The maltose regulon of Vibrio cholerae affects production and secretion of virulence factors.
Lång, H, Jonson, G, Holmgren, J, Palva, E T
The effects of maltose on production and secretion of virulence factors of Vibrio cholerae in strain X28214, classical biotype, and in maltose-defective transposon mutants constructed from this...
Raghavan, S., Nyström, J., Fredriksson, M., Holmgren, J., Harandi, A. M.
Bacterial DNA and unmethylated CpG oligodeoxynucleotides (CpG ODN) are known to be potent stimulators of the innate immune system in vitro and in vivo. We therefore investigated if oral...
Czerkinsky, C, Russell, M W, Lycke, N, Lindblad, M, Holmgren, J
Generation of local and systemic immune responses by the oral administration of antigens is frequently inefficient, requiring large quantities of immunogens and yielding only modest antibody...
A protocol was elaborated for the adoptive transfer of lymphocytes from mice which were orally immunized with cholera toxin (CT) to enable the study of long-term gut mucosal immunological memory at...
Jonson, G., Svennerholm, A. M., Holmgren, J.
Vibrio cholerae O1 bacteria harvested directly from ligated or nonligated intestines of rabbits with experimental cholera expressed at least 7 to 8 novel, in vivo-specific cell envelope (env)...
Holmgren, J, Fredman, P, Lindblad, M, Svennerholm, A M, Svennerholm, L
The receptors for cholera toxin and Escherichia coli heat-labile toxin (LT) in rabbit small intestinal epithelium were characterized and compared. (i) In vivo studies in ligated intestinal loops...
Holmgren, J, Svennerholm, A M, Lindblad, M
The two biotypes of Vibrio cholerae were found to have cell-associated hemagglutinins which differ with regard to binding to different species of erythrocytes and inhibition by monosaccharides. A...
Holmgren, J, Svennerholm, L, Elwing, H, Fredman, P, Strannegård, O
The binding of Sendai virus to polystyrene petri dishes to which various gangliosides of defined structures had been adsorbed was determined. The ganglioside-bound virus was visualized either by a...
Holmgren, J, Svennerholm, A M, Ahrén, C
Human milk and colostrum samples were divided into an immunoglobulin and a nonimmunoglobulin fraction by immunosorbent chromatography. The ability of these fractions to inhibit bacterial cell...
Hirst, T R, Sanchez, J, Kaper, J B, Hardy, S J, Holmgren, J
A genetically engineered Vibrio cholerae strain from which the cholera toxin genes had previously been deleted was used as a host in which to study the expression and secretion of related toxins and...
Holmgren, J, Svennerholm, A M, Ouchterlony, O, Anderson, A, Walletström, G, Westerberg-Berndtsson, U
The protective effect of enternal and parenteral immunization with cholera toxin antigen against experimental cholera in rabbits was studied by using the small-bowel loop technique. Subcutaneous...
Holmgren, J., Lönnroth, I., Ouchterlony, Ö.
Two crude toxin preparations of Vibrio cholerae, labeled lot 4493 G (Inaba) and lot 001 (Ogawa), consisting of freeze-dried culture filtrate, were studied with regard to toxicity,...
Subcutaneous immunization of rabbits with a combination of Vibrio cholerae lipopolysaccharide (LPS) and enterotoxin induced a more than 100-fold-higher degree of protection against intestinal...
An in vitro culture technique with synthesis of 14C-labeled protein has been used to study immunoglobulin and specific-antibody formation by spleen, mesenteric lymph nodes, Peyer's patches, and small...
Svennerholm, A, Lange, S, Holmgren, J
The importance of locally and systemically formed antibodies of various classes for protection against experimental cholera has been studied in mice immunized with cholera toxin. Groups of mice were...
Holmgren, J., Andersson, Å., Wallerstrom, G., Ouchterlony, Ö.
By use of the ileal loop technique, the resistance to challenge with cholera enterotoxin was compared between unimmunized rabbits and rabbits immunized with toxin or toxoids. It was shown that...
Enzyme-Linked Immunosorbent Assays for Cholera Serology
The enzyme-linked immunosorbent assay (ELISA) principle of Engvall and Perlmann, which employs antigen-coated tubes and enzyme-labeled anti-immunoglobulins, was elaborated for use in cholera...
Holmgren, J., Lönnroth, I., Svennerholm, L.
By a double-diffusion precipitation-in-gel technique, isolated cholera toxin as well as its natural toxoid were shown to be fixed and precipitated by the ganglioside GM1 but not by any of the related...
Local Antibody Production in Experimental Pyelonephritis: Amount, Avidity, and Immunoglobulin Class
Smith, J., Holmgren, J., Ahlstedt, S., Hanson, L. A.
Local antibody formation in infected rabbit kidneys was studied with three techniques: the ammonium sulfate precipitation technique, the enzyme-linked immunosorbent assay, and by binding of newly...
Ultrastructural localization of cell membrane GM1 ganglioside by cholera toxin
An immunoelectron microscopic method is described for sensitive high-resolution visualization of tissuebound cholera toxin. The principle is to incubate cells or tissue sections with toxin and then...
Interaction of cholera toxin and membrane GM1 ganglioside of small intestine.
Holmgren, J, Lönnroth, I, Månsson, J, Svennerholm, L
Ganglioside GM1 was isolated from the small intestinal mucosa of man, pig, and beef and amounted to 0.1, 2.0, and 43 nmol per g fresh weight, respectively. These differences in GM1 content were...
Quiding-Järbrink, M, Nordström, I, Granström, G, Kilander, A, Jertborn, M, Butcher, E C, ...
Expression of the adhesion molecules CD44, L-selectin (CD62L), and integrin alpha 4 beta 7 by antibody-secreting cells (ASC) was examined in human volunteers after oral, rectal, intranasal, or...
Svennerholm, A M, Lange, S, Holmgren, J
The influence in immunization with cholera toxin of the route and antigen dose on intestinal antibody formation and protective immunity against experimental cholera was studied in mice....
Svennerholm, A M, Hanson, L A, Holmgren, J, Lindblad, B S, Nilsson, B, Quereshi, F
The capacity of subcutaneous cholera vaccination to induce an antibody response in milk and saliva was studied in lactating Swedish and Pakistani women, since secretory immunoglobulin A (SIgA)...
Detection by monoclonal antibody of carbohydrate antigen CA 50 in serum of patients with carcinoma.
Holmgren, J, Lindholm, L, Persson, B, Lagergård, T, Nilsson, O, Svennerholm, L, ...
A solid phase radioimmunoassay was devised for measuring the value of the carcinoma associated carbohydrate antigen CA 50 in serum based on the use of a specific monoclonal antibody (C 50). Samples...
Rabbani, G H, Greenough, W B, Holmgren, J, Kirkwood, B
A randomised controlled trial was conducted to investigate the ability of chlorpromazine to reduce intestinal secretion in cholera. Chlorpromazine had reduced loss of intestinal fluid in animals with...
Letter: New enterotoxinogenic bacteria isolated.
Wadström, T, Kettis, A A, Habte, D, Holmgren, J, Meeuwisse, G, Möllby, R, ...
Ahlstedt, S., Holmgren, J., Hanson, L. Å.
Determinations of antibody titres with the ammonium sulphate precipitation technique showed an accuracy of ± 10 per cent (P
Ahlstedt, S., Holmgren, J., Hanson, L. Å.
The primary and secondary antibody response in inbred CBA mice against Escherichia coli O6 lipopolysaccharide was investigated. The avidity was found to be inversely related to the immunization dose....
Exposure of rabbits and mice to serologically different E. coli bacteria enhanced the synthesis of IgG but often suppressed the formation of IgM O antibodies in response to subsequent immunization...
In the present study in mice we used the Jerne plaque assay to compare the immunity enhancing potential of different Gram-negative bacteria with special regard to their endotoxin. The results confirm...
There is a great need for substances that can act as adjuvants on local mucosal immune responses to perorally (p.o.) administered immunogens and which could be included in future oral vaccines. In...
Human gastric B cell responses can be induced by intestinal immunisation
Quiding-Jarbrink, M, Lonroth, H, Ahlstedt, I, Holmgren, J, Svennerholm, A
BACKGROUND—In a previous study, we found that oral vaccination induces strong B cell responses in the stomach of Helicobacter pylori infected but not of uninfected individuals. In this study, we...
Friman, V., Quiding, M., Czerkinsky, C., Nordström, I., Larsson, L., Ericson, D., ...
In search for a possible explanation for the different susceptibility to mucosal infections in IgA-deficient (IgAd) individuals, the frequency of total immunoglobulin-secreting cells (ISC) and...
Quiding, M, Granström, G, Nordström, I, Ferrua, B, Holmgren, J, Czerkinsky, C
The frequency of mononuclear cells (MNC) spontaneously secreting interferon-gamma (IFN-gamma) has been examined in freshly isolated cell suspensions from human palatine tonsils. Two-site reverse...
Lycke, N, Lindholm, L, Holmgren, J
We have studied specific antibody production from peripheral blood lymphocytes (PBL) after oral cholera immunization of humans and mice. Two oral immunizations with cholera toxin (CT) in mice or a...
Hanson, L. Å., Holmgren, J., Jodal, U., Kaijser, B.
Precipitating serum antibodies to Escherichia coli O antigens from some infants and children with acute pyelonephritis were studied.
RAGHAVAN, S, FREDRIKSSON, M, SVENNERHOLM, A-M, HOLMGREN, J, SURI-PAYER, E
Helicobacter pylori induces symptomatic chronic gastritis in a subpopulation of infected individuals. The mechanism(s) determining the development and severity of pathology leading to symptoms are...
STANFORD, M, WHITTALL, T, BERGMEIER, L A, LINDBLAD, M, LUNDIN, S, SHINNICK, T, ...
Behcet's disease (BD) specific peptide (p336–351) was identified within the human 60 kD heat shock protein (HSP60). Oral p336–351 induced uveitis in rats which was prevented by oral tolerization...
Eriksson, K, Nordström, I, Czerkinsky, C, Holmgren, J
We have studied how cholera toxin (CT) and its non-toxic cell-binding B-subunit (CTB) affect the activation of pure human T cells in an anti-CD3-driven system. CT, as opposed to CTB, strongly...
Wadström, T, Aust-Kettis, A, Habte, D, Holmgren, J, Meeuwisse, G, Möllby, R, ...
Enterotoxinogenic bacteria were isolated from 131 (37%) of 354 Ethiopian infants and children with acute gastrointestinal symptoms. Only one of these isolates belonged to the classical...
Holmgren, J., Lindholm, L., Lönnroth, I.
The interaction of cholera toxin and a number of toxin derivatives, containing different proportions of light and heavy toxin-composing subunits (L and H), with mouse lymphocytes was studied....
Distribution of lymphocytes and adhesion molecules in human cervix and vagina
Johansson, E-L, Rudin, A, Wassén, L, Holmgren, J
Knowledge of the histological distribution of leucocytes and adhesion molecules in the human genital tract is scarce although local immunity in this region is important. Using immunohistochemical...
Enterotoxin antibodies in relation to diarrhoea in Swedish soldiers in Cyprus
The development of antibodies to Escherichia coli heat-labile enterotoxin (LT) and its relationship to diarrhoea was studied in two Swedish battalions (one in the winter, WB, and one in the summer,...
Gothefors, L., Sack, D. A., Bardhan, P. K., Holmgren, J.
A single oral or intramuscular immunization with purified cholera B subunit induced an intestinal secretory immunoglobulin A (IgA) antitoxin response in, respectively, 10 out of 11 and 9 out of 12...
Trach, D. D., Cam, P. D., Ke, N. T., Rao, M. R., Dinh, D., Hang, P. V., ...
OBJECTIVE: To evaluate a killed oral cholera vaccine produced in Viet Nam, and to compare the Vietnamese vaccine with one that is licensed internationally. METHOD: Two-dose regimens of a locally...