J. I. Gordon

NIGHT AERIAL SEARCH FOR AVIATORS DOWNED AT SEA (OPTIMUM SEARCH PROCEDURES), (2005)

GORDON,J. I., Church,P. V., Wasserboehr,J. W., Duntley,S. Q.

Navy tests have demonstrated that the helmet of a Naval aviator downed and afloat at night can be sighted from a searchlight-equipped rescue helicopter if the helmet is covered with a retrodirective...

BIOINFORMATICS ORIGINAL PAPER Genome (2004)

B. P. Westover, J. D. Buhler, J. L. Sonnenburg, J. I. Gordon

Motivation: Annotation of operons in a bacterial genome is an important step in determining an organism’s transcriptional regulatory program. While extensive studies of operon structure have been...

Operon prediction without a training set (2004)

Westover, B. P., Buhler, J. D., Sonnenburg, J. L., Gordon, J. I.

Motivation: Annotation of operons in a bacterial genome is an important step in determining an organism's transcriptional regulatory program. While extensive studies of operon structure have been...

Operon prediction without a training set (2004)

Westover, B. P., Buhler, J. D., Sonnenburg, J. L., Gordon, J. I.

Motivation: Annotation of operons in a bacterial genome is an important step in determining an organism's transcriptional regulatory program. While extensive studies of operon structure have been...

Equilibrium Radiance Model Applications and Comparisons to Atmospheric Measurements and Rayleigh Models, (2002)

Gordon,J. I., Johnson,R. W.

The equilibrium radiance model, which assumes constancy in the source function along the path of sight, predicts equilibrium radiance as a function of the angle from the sun. Measurements made at all...

Selection of multipotent stem cells during morphogenesis of small intestinal crypts of Lieberkuhn is perturbed by stimulation of Lef-1/beta-catenin signaling (2002)

Wong, M. H., Huelsken, J., Birchmeier, W., Gordon, J. I.

Studies of chimeric mice have disclosed that the stem cell hierarchy in the small intestinal epithelium is established during formation of its proliferative units (crypts of Lieberkuhn). This process...

Phenotype of mice lacking functional Deleted in colorectal cancer (Dcc) gene. (1997)

Fazeli, A, Dickinson, S L, Hermiston, M L, Tighe, R V, Steen, R G, Small, C G, ...

The DCC (Deleted in colorectal cancer) gene was first identified as a candidate for a tumour-suppressor gene on human chromosome 18q. More recently, in vitro studies in rodents have provided evidence...

Forced expression of the tumor suppressor adenomatosis polyposis coli protein induces disordered cell migration in the intestinal epithelium.

Wong, M H, Hermiston, M L, Syder, A J, Gordon, J I

Mutations of the human adenomatosis polyposis coli (APC) gene are associated with the development of familial as well as sporadic intestinal neoplasia. To examine the in vivo function of APC, 129/Sv...

A 20-nucleotide element in the intestinal fatty acid binding protein gene modulates its cell lineage-specific, differentiation-dependent, and cephalocaudal patterns of expression in transgenic mice.

Simon, T C, Roberts, L J, Gordon, J I

A sequence of epithelial cell proliferation, allocation to four principal lineages, migration-associated differentiation, and cell loss occurs along the crypt-villus axis of the mouse intestine. The...

Expression of a human alpha-1,3/4-fucosyltransferase in the pit cell lineage of FVB/N mouse stomach results in production of Leb-containing glycoconjugates: a potential transgenic mouse model for studying Helicobacter pylori infection.

Falk, P G, Bry, L, Holgersson, J, Gordon, J I

Helicobacter pylori is a human pathogen associated with the development of gastric and duodenal ulcers and gastric adenocarcinoma. To test the hypothesis that the human Lewis(b) blood group antigen...

Expression of wild-type and mutant simian virus 40 large tumor antigens in villus-associated enterocytes of transgenic mice.

Kim, S H, Roth, K A, Coopersmith, C M, Pipas, J M, Gordon, J I

The four principal gut epithelial cell lineages undergo continuous and rapid renewal during a geographically well-organized migration along the crypt-to-villus axis. The molecules that regulate their...

Toxicity of myristic acid analogs toward African trypanosomes.

Doering, T L, Lu, T, Werbovetz, K A, Gokel, G W, Hart, G W, Gordon, J I, ...

New drugs are needed for treatment of diseases caused by African trypanosomes. One possible target for chemotherapy is the biosynthesis of the glycosyl phosphatidyl-inositol (GPI) of this parasite's...

Suppressors of nmtl-181, a conditional lethal allele of the Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase gene, reveal proteins involved in regulating protein N-myristoylation.

Johnson, D R, Cok, S J, Feldmann, H, Gordon, J I

Several essential Saccharomyces cerevisiae proteins require myristate to be covalently bound to their amino-terminal glycine for biological activity. Protein N-myristoylation is catalyzed by...

Paneth cell differentiation in the developing intestine of normal and transgenic mice.

Bry, L, Falk, P, Huttner, K, Ouellette, A, Midtvedt, T, Gordon, J I

Paneth cells represent one of the four major epithelial lineages in the mouse small intestine. It is the only lineage that migrates downward from the stem-cell zone located in the lower portion of...

Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans.

Lodge, J K, Jackson-Machelski, E, Toffaletti, D L, Perfect, J R, Gordon, J I

Cryptococcus neoformans is a major cause of systemic fungal infection in immunocompromised patients. Myristoyl-CoA:protein N-myristoyltransferase (Nmt) catalyzes the transfer of myristate (C14:0)...

An in vitro adherence assay reveals that Helicobacter pylori exhibits cell lineage-specific tropism in the human gastric epithelium.

Falk, P, Roth, K A, Borén, T, Westblom, T U, Gordon, J I, Normark, S

Helicobacter pylori is a microaerophilic bacterium found in the stomach of asymptomatic humans as well as patients with acid peptic disease and gastric adenocarcinoma. We have developed an in situ...

Chimeric-transgenic mice represent a powerful tool for studying how the proliferation and differentiation programs of intestinal epithelial cell lineages are regulated.

Hermiston, M L, Green, R P, Gordon, J I

An in vivo system has been developed for examining the effects of wild-type or mutant proteins on cell fate determination in the mouse intestinal epithelium or on the proliferation and...

Cell lineage-specific and differentiation-dependent patterns of CCAAT/enhancer binding protein alpha expression in the gut epithelium of normal and transgenic mice.

Chandrasekaran, C, Gordon, J I

The proliferation and differentiation programs of gut epithelial cells are expressed rapidly and perpetually along an anatomically well defined pathway. The mouse intestine thus provides an excellent...

Analogs of palmitoyl-CoA that are substrates for myristoyl-CoA:protein N-myristoyltransferase.

Rudnick, D A, Lu, T, Jackson-Machelski, E, Hernandez, J C, Li, Q, Gokel, G W, ...

Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase (Nmt1p; EC 2.3.1.97) is an essential enzyme that is highly selective for myristoyl-CoA in vivo. It is unclear why myristate...

Temporal and spatial patterns of transgene expression in aging adult mice provide insights about the origins, organization, and differentiation of the intestinal epithelium.

Cohn, S M, Roth, K A, Birkenmeier, E H, Gordon, J I

We have used liver fatty acid-binding protein/human growth hormone (L-FABP/hGH) fusion genes to explore the temporal and spatial differentiation of intestinal epithelial cells in 1- to 12-month-old...

Incorporation of 12-methoxydodecanoate into the human immunodeficiency virus 1 gag polyprotein precursor inhibits its proteolytic processing and virus production in a chronically infected human lymphoid cell line.

Bryant, M L, Ratner, L, Duronio, R J, Kishore, N S, Devadas, B, Adams, S P, ...

Covalent linkage of myristate (tetradecanoate; 14:0) to the NH2-terminal glycine residue of the human immunodeficiency virus 1 (HIV-1) 55-kDa gag polyprotein precursor (Pr55gag) is necessary for its...

Use of transgenic mice to infer the biological properties of small intestinal stem cells and to examine the lineage relationships of their descendants.

Roth, K A, Hermiston, M L, Gordon, J I

Transgenes, composed of elements of the 5' nontranscribed region of the liver fatty acid-binding protein (L-FABP) gene linked to various reporters, have previously been used to explore the cellular,...

G-protein alpha-subunit expression, myristoylation, and membrane association in COS cells.

Mumby, S M, Heukeroth, R O, Gordon, J I, Gilman, A G

Myristoylation of seven different alpha subunits of guanine nucleotide-binding regulatory proteins (G proteins) was examined by expressing these proteins in monkey kidney COS cells. Metabolic...

Protein N-myristoylation in Escherichia coli: reconstitution of a eukaryotic protein modification in bacteria.

Duronio, R J, Jackson-Machelski, E, Heuckeroth, R O, Olins, P O, Devine, C S, Yonemoto, W, ...

Protein N-myristoylation refers to the covalent attachment of a myristoyl group (C14:0), via amide linkage, to the NH2-terminal glycine residue of certain cellular and viral proteins....

Spatial differentiation of the intestinal epithelium: analysis of enteroendocrine cells containing immunoreactive serotonin, secretin, and substance P in normal and transgenic mice.

Roth, K A, Gordon, J I

The mammalian intestinal epithelium undergoes continuous and rapid renewal of its four principal terminally differentiated cell types. These cells arise from multipotent stem cells located at or near...

Functional analysis of protein N-myristoylation: metabolic labeling studies using three oxygen-substituted analogs of myristic acid and cultured mammalian cells provide evidence for protein-sequence-specific incorporation and analog-specific redistribution.

Johnson, D R, Cox, A D, Solski, P A, Devadas, B, Adams, S P, Leimgruber, R M, ...

Covalent attachment of myristic acid (C14:0) to the NH2-terminal glycine residue of a number of cellular, viral, and oncogene-encoded proteins is essential for full expression of their biological...

Transcription of INO2 and INO4 is regulated by the state of protein N-myristoylation in Saccharomyces cerevisiae.

Cok, S J, Martin, C G, Gordon, J I

Inositol regulates transcription of Saccharomyces cerevisiae genes required for de novo synthesis of acylCoAs and phospholipids. Removal of inositol results in transcriptional activation by...

Expression of thin aggregative fimbriae promotes interaction of Salmonella typhimurium SR-11 with mouse small intestinal epithelial cells.

Sukupolvi, S, Lorenz, R G, Gordon, J I, Bian, Z, Pfeifer, J D, Normark, S J, ...

The factors that mediate binding of Salmonella typhimurium to small intestinal epithelial cells have not been fully characterized. In this paper we demonstrate that elimination of production of thin...

The hypothyroid (hyt/hyt) mouse: a model system for studying the effects of thyroid hormone on developmental changes in gene expression.

Green, R P, Birkenmeier, E H, Beamer, W G, Maltais, L J, Gordon, J I

Thyroid hormone has been implicated as an important factor in rodent development. We have used a strain of mice with a recessive mutation producing congenital primary hypothyroidism (C.RF/J-hyt/+) to...

Heteroatom-substituted fatty acid analogs as substrates for N-myristoyltransferase: an approach for studying both the enzymology and function of protein acylation.

Heuckeroth, R O, Glaser, L, Gordon, J I

Myristoyl-CoA:protein N-myristoyltransferase (NMT), the enzyme that transfers the myristoyl (14:0) moiety from myristoyl CoA thioester to nascent proteins, is remarkably specific for both peptide and...

Transgenic mice containing intestinal fatty acid-binding protein-human growth hormone fusion genes exhibit correct regional and cell-specific expression of the reporter gene in their small intestine.

Sweetser, D A, Hauft, S M, Hoppe, P C, Birkenmeier, E H, Gordon, J I

The rat intestinal fatty acid binding protein (I-FABP) gene exhibits cell-specific as well as regional differences in its expression within the continuously regenerating small intestinal epithelium....

Cellular differentiation in the emerging fetal rat small intestinal epithelium: mosaic patterns of gene expression.

Rubin, D C, Ong, D E, Gordon, J I

We have examined the pattern of differentiation of the small intestinal epithelium in fetal rats during the 17th through 21st days of gestation. Five genes expressed in late fetal, neonatal, and...

Altered membrane association of p60v-src and a murine 63-kDa N-myristoyl protein after incorporation of an oxygen-substituted analog of myristic acid.

Heuckeroth, R O, Gordon, J I

A number of viral and cellular proteins contain covalently bound lipid. In a subset of these acyl proteins, the 14-carbon saturated fatty acid myristic acid is attached through an amide linkage to an...

Refined apoprotein structure of rat intestinal fatty acid binding protein produced in Escherichia coli.

Sacchettini, J C, Gordon, J I, Banaszak, L J

Rat intestinal fatty acid binding protein (I-FABP) is a member of a family of cytoplasmic hydrophobic ligand-binding proteins. To gain insights about the contribution of bound fatty acid to I-FABP's...

Replication of human immunodeficiency virus 1 and Moloney murine leukemia virus is inhibited by different heteroatom-containing analogs of myristic acid.

Bryant, M L, Heuckeroth, R O, Kimata, J T, Ratner, L, Gordon, J I

Myristoyl-CoA:protein N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the cotranslational linkage of myristate to the N-terminal glycine residues of several cellular, viral, and oncoproteins. We...

Rat intestinal apolipoprotein B gene expression. Evidence for integrated regulation by bile salt, fatty acid, and phospholipid flux.

Davidson, N O, Drewek, M J, Gordon, J I, Elovson, J

We previously reported that intestinal apo B48 synthesis in the rat was unaltered by dietary triglyceride intake but demonstrated regulation in response to biliary lipid availability. Studies are now...

Globin gene expression in erythroid human fetal liver cells.

Ley, T J, Maloney, K A, Gordon, J I, Schwartz, A L

We measured steady-state levels of the human globin mRNAs in liver samples from several mid-gestational fetuses. RNA from the epsilon, gamma, beta, zeta, theta, and alpha globin genes were present in...

Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase.

Towler, D A, Adams, S P, Eubanks, S R, Towery, D S, Jackson-Machelski, E, Glaser, L, ...

Myristoyl CoA:protein N-myristoyltransferase (NMT) catalyzes the addition of myristic acid to the amino-terminal glycine residues of a number of eukaryotic proteins. Recently, we developed a...

Developmental regulation of a gene that encodes a cysteine-rich intestinal protein and maps near the murine immunoglobulin heavy chain locus.

Birkenmeier, E H, Gordon, J I

Mouse and rat small intestinal cDNA libraries were screened for recombinants derived from mRNAs whose concentration changed during the transition from suckling to weaning. cDNAs transcribed from a...

Cloning of a cDNA encoding rat intestinal fatty acid binding protein.

Alpers, D H, Strauss, A W, Ockner, R K, Bass, N M, Gordon, J I

Intestinal fatty acid binding protein mRNA is one of the most abundant mRNA species in the rat small intestinal epithelium. RNA transfer blot analyses disclosed that the mRNA encoding intestinal...

Coordinate regulation of two estrogen-dependent genes in avian liver.

Wiskocil, R, Bensky, P, Dower, W, Goldberger, R F, Gordon, J I, Deeley, R G

Livers of egg-laying species contain abundant mRNAs encoded by both estrogen-responsive and constitutively expressed genes. We have recently constructed cDNA clones from three members of the abundant...

Rat cellular retinol-binding protein II: use of a cloned cDNA to define its primary structure, tissue-specific expression, and developmental regulation.

Li, E, Demmer, L A, Sweetser, D A, Ong, D E, Gordon, J I

The primary structure of rat cellular retinol-binding protein (CRBP) II has been determined from a cloned cDNA. Alignment of this 134-amino acid, 15,580-Da polypeptide with rat CRBP revealed that 75...

Tissue-specific expression and developmental regulation of the rat apolipoprotein B gene.

Demmer, L A, Levin, M S, Elovson, J, Reuben, M A, Lusis, A J, Gordon, J I

Expression of the apolipoprotein B (apoB) gene was examined in a variety of fetal, neonatal, and adult rat tissues by probing RNA blots with a cloned rat apoB cDNA. Among 10 adult male tissues...

Expression of rat apolipoprotein A-IV and A-I genes: mRNA induction during development and in response to glucocorticoids and insulin.

Elshourbagy, N A, Boguski, M S, Liao, W S, Jefferson, L S, Gordon, J I, Taylor, J M

Rat apolipoproteins (apo-) A-IV and A-I share many structural similarities, the most notable of which is a domain of repeated docosapeptides with amphipathic helical potential. Although the genes for...

Rat apolipoprotein A-IV contains 13 tandem repetitions of a 22-amino acid segment with amphipathic helical potential.

Boguski, M S, Elshourbagy, N, Taylor, J M, Gordon, J I

Apolipoprotein (apo) A-IV is a 46,000 Mr protein that is a major component of rat high density lipoproteins and chylomicrons. We have isolated, from a rat intestinal cDNA library, a full-length apo...

Primary induction of vitellogenin mRNA in the rooster by 17beta-estradiol.

Burns, A T, Deeley, R G, Gordon, J I, Udell, D S, Mullinix, K P, Goldberger, R F

We have studied the kinetics of vitellogenin mRNA accumulation in rooster liver after a primary injection of 17beta-estradiol. The levels of vitellogenin mRNA have been determined both by...

Comparative analysis of repeated sequences in rat apolipoproteins A-I, A-IV, and E.

Boguski, M S, Elshourbagy, N, Taylor, J M, Gordon, J I

To understand the structural, functional, and evolutionary relationships among the principal protein components of rat high density lipoprotein particles, we undertook a systematic comparative...

Comparative in vitro study of the pro-apolipoprotein A-I to apolipoprotein A-I converting activity between normal and Tangier plasma.

Edelstein, C, Gordon, J I, Vergani, C A, Catapano, A L, Pietrini, V, Scanu, A M

We examined the ability of the plasma of a 52-yr-old male Tangier patient to effect the conversion of radiolabeled pro-apolipoprotein A-I (apo A-I), isolated from hepatoma cell culture media, into...

Induction of vitellogenin synthesis by estrogen in avian liver: relationship between level of vitellogenin mRNA and vitellogenin synthesis.

Mullinix, K P, Wetekam, W, Deeley, R G, Gordon, J I, Meyers, M, Kent, K A, ...

We have investigated the estrogen-mediated induction of vitellogenin synthesis in rooster liver. We compared the concentrations of vitellogenin messenger RNA (mRNA) in the liver with the...

Mutations of human myristoyl-CoA:protein N-myristoyltransferase cause temperature-sensitive myristic acid auxotrophy in Saccharomyces cerevisiae.

Duronio, R J, Reed, S I, Gordon, J I

We have isolated cDNAs encoding human myristoyl-CoA:protein N-myristoyltransferase (NMT, EC 2.3.1.97) by complementing the nmtl-181 mutation of Saccharomyces cerevisiae, which causes...

Forced expression of the tumor suppressor adenomatosis polyposis coli protein induces disordered cell migration in the intestinal epithelium.

Wong, M H, Hermiston, M L, Syder, A J, Gordon, J I

Mutations of the human adenomatosis polyposis coli (APC) gene are associated with the development of familial as well as sporadic intestinal neoplasia. To examine the in vivo function of APC, 129/Sv...

A 20-nucleotide element in the intestinal fatty acid binding protein gene modulates its cell lineage-specific, differentiation-dependent, and cephalocaudal patterns of expression in transgenic mice.

Simon, T C, Roberts, L J, Gordon, J I

A sequence of epithelial cell proliferation, allocation to four principal lineages, migration-associated differentiation, and cell loss occurs along the crypt-villus axis of the mouse intestine. The...

Expression of a human alpha-1,3/4-fucosyltransferase in the pit cell lineage of FVB/N mouse stomach results in production of Leb-containing glycoconjugates: a potential transgenic mouse model for studying Helicobacter pylori infection.

Falk, P G, Bry, L, Holgersson, J, Gordon, J I

Helicobacter pylori is a human pathogen associated with the development of gastric and duodenal ulcers and gastric adenocarcinoma. To test the hypothesis that the human Lewis(b) blood group antigen...

Expression of wild-type and mutant simian virus 40 large tumor antigens in villus-associated enterocytes of transgenic mice.

Kim, S H, Roth, K A, Coopersmith, C M, Pipas, J M, Gordon, J I

The four principal gut epithelial cell lineages undergo continuous and rapid renewal during a geographically well-organized migration along the crypt-to-villus axis. The molecules that regulate their...

Toxicity of myristic acid analogs toward African trypanosomes.

Doering, T L, Lu, T, Werbovetz, K A, Gokel, G W, Hart, G W, Gordon, J I, ...

New drugs are needed for treatment of diseases caused by African trypanosomes. One possible target for chemotherapy is the biosynthesis of the glycosyl phosphatidyl-inositol (GPI) of this parasite's...

Suppressors of nmtl-181, a conditional lethal allele of the Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase gene, reveal proteins involved in regulating protein N-myristoylation.

Johnson, D R, Cok, S J, Feldmann, H, Gordon, J I

Several essential Saccharomyces cerevisiae proteins require myristate to be covalently bound to their amino-terminal glycine for biological activity. Protein N-myristoylation is catalyzed by...

Paneth cell differentiation in the developing intestine of normal and transgenic mice.

Bry, L, Falk, P, Huttner, K, Ouellette, A, Midtvedt, T, Gordon, J I

Paneth cells represent one of the four major epithelial lineages in the mouse small intestine. It is the only lineage that migrates downward from the stem-cell zone located in the lower portion of...

Targeted gene replacement demonstrates that myristoyl-CoA: protein N-myristoyltransferase is essential for viability of Cryptococcus neoformans.

Lodge, J K, Jackson-Machelski, E, Toffaletti, D L, Perfect, J R, Gordon, J I

Cryptococcus neoformans is a major cause of systemic fungal infection in immunocompromised patients. Myristoyl-CoA:protein N-myristoyltransferase (Nmt) catalyzes the transfer of myristate (C14:0)...

An in vitro adherence assay reveals that Helicobacter pylori exhibits cell lineage-specific tropism in the human gastric epithelium.

Falk, P, Roth, K A, Borén, T, Westblom, T U, Gordon, J I, Normark, S

Helicobacter pylori is a microaerophilic bacterium found in the stomach of asymptomatic humans as well as patients with acid peptic disease and gastric adenocarcinoma. We have developed an in situ...

Chimeric-transgenic mice represent a powerful tool for studying how the proliferation and differentiation programs of intestinal epithelial cell lineages are regulated.

Hermiston, M L, Green, R P, Gordon, J I

An in vivo system has been developed for examining the effects of wild-type or mutant proteins on cell fate determination in the mouse intestinal epithelium or on the proliferation and...

Cell lineage-specific and differentiation-dependent patterns of CCAAT/enhancer binding protein alpha expression in the gut epithelium of normal and transgenic mice.

Chandrasekaran, C, Gordon, J I

The proliferation and differentiation programs of gut epithelial cells are expressed rapidly and perpetually along an anatomically well defined pathway. The mouse intestine thus provides an excellent...

Analogs of palmitoyl-CoA that are substrates for myristoyl-CoA:protein N-myristoyltransferase.

Rudnick, D A, Lu, T, Jackson-Machelski, E, Hernandez, J C, Li, Q, Gokel, G W, ...

Saccharomyces cerevisiae myristoyl-CoA:protein N-myristoyltransferase (Nmt1p; EC 2.3.1.97) is an essential enzyme that is highly selective for myristoyl-CoA in vivo. It is unclear why myristate...

Temporal and spatial patterns of transgene expression in aging adult mice provide insights about the origins, organization, and differentiation of the intestinal epithelium.

Cohn, S M, Roth, K A, Birkenmeier, E H, Gordon, J I

We have used liver fatty acid-binding protein/human growth hormone (L-FABP/hGH) fusion genes to explore the temporal and spatial differentiation of intestinal epithelial cells in 1- to 12-month-old...

Incorporation of 12-methoxydodecanoate into the human immunodeficiency virus 1 gag polyprotein precursor inhibits its proteolytic processing and virus production in a chronically infected human lymphoid cell line.

Bryant, M L, Ratner, L, Duronio, R J, Kishore, N S, Devadas, B, Adams, S P, ...

Covalent linkage of myristate (tetradecanoate; 14:0) to the NH2-terminal glycine residue of the human immunodeficiency virus 1 (HIV-1) 55-kDa gag polyprotein precursor (Pr55gag) is necessary for its...

Use of transgenic mice to infer the biological properties of small intestinal stem cells and to examine the lineage relationships of their descendants.

Roth, K A, Hermiston, M L, Gordon, J I

Transgenes, composed of elements of the 5' nontranscribed region of the liver fatty acid-binding protein (L-FABP) gene linked to various reporters, have previously been used to explore the cellular,...

G-protein alpha-subunit expression, myristoylation, and membrane association in COS cells.

Mumby, S M, Heukeroth, R O, Gordon, J I, Gilman, A G

Myristoylation of seven different alpha subunits of guanine nucleotide-binding regulatory proteins (G proteins) was examined by expressing these proteins in monkey kidney COS cells. Metabolic...

Protein N-myristoylation in Escherichia coli: reconstitution of a eukaryotic protein modification in bacteria.

Duronio, R J, Jackson-Machelski, E, Heuckeroth, R O, Olins, P O, Devine, C S, Yonemoto, W, ...

Protein N-myristoylation refers to the covalent attachment of a myristoyl group (C14:0), via amide linkage, to the NH2-terminal glycine residue of certain cellular and viral proteins....

Spatial differentiation of the intestinal epithelium: analysis of enteroendocrine cells containing immunoreactive serotonin, secretin, and substance P in normal and transgenic mice.

Roth, K A, Gordon, J I

The mammalian intestinal epithelium undergoes continuous and rapid renewal of its four principal terminally differentiated cell types. These cells arise from multipotent stem cells located at or near...

Functional analysis of protein N-myristoylation: metabolic labeling studies using three oxygen-substituted analogs of myristic acid and cultured mammalian cells provide evidence for protein-sequence-specific incorporation and analog-specific redistribution.

Johnson, D R, Cox, A D, Solski, P A, Devadas, B, Adams, S P, Leimgruber, R M, ...

Covalent attachment of myristic acid (C14:0) to the NH2-terminal glycine residue of a number of cellular, viral, and oncogene-encoded proteins is essential for full expression of their biological...

Transcription of INO2 and INO4 is regulated by the state of protein N-myristoylation in Saccharomyces cerevisiae.

Cok, S J, Martin, C G, Gordon, J I

Inositol regulates transcription of Saccharomyces cerevisiae genes required for de novo synthesis of acylCoAs and phospholipids. Removal of inositol results in transcriptional activation by...

Expression of thin aggregative fimbriae promotes interaction of Salmonella typhimurium SR-11 with mouse small intestinal epithelial cells.

Sukupolvi, S, Lorenz, R G, Gordon, J I, Bian, Z, Pfeifer, J D, Normark, S J, ...

The factors that mediate binding of Salmonella typhimurium to small intestinal epithelial cells have not been fully characterized. In this paper we demonstrate that elimination of production of thin...

The hypothyroid (hyt/hyt) mouse: a model system for studying the effects of thyroid hormone on developmental changes in gene expression.

Green, R P, Birkenmeier, E H, Beamer, W G, Maltais, L J, Gordon, J I

Thyroid hormone has been implicated as an important factor in rodent development. We have used a strain of mice with a recessive mutation producing congenital primary hypothyroidism (C.RF/J-hyt/+) to...

Heteroatom-substituted fatty acid analogs as substrates for N-myristoyltransferase: an approach for studying both the enzymology and function of protein acylation.

Heuckeroth, R O, Glaser, L, Gordon, J I

Myristoyl-CoA:protein N-myristoyltransferase (NMT), the enzyme that transfers the myristoyl (14:0) moiety from myristoyl CoA thioester to nascent proteins, is remarkably specific for both peptide and...

Transgenic mice containing intestinal fatty acid-binding protein-human growth hormone fusion genes exhibit correct regional and cell-specific expression of the reporter gene in their small intestine.

Sweetser, D A, Hauft, S M, Hoppe, P C, Birkenmeier, E H, Gordon, J I

The rat intestinal fatty acid binding protein (I-FABP) gene exhibits cell-specific as well as regional differences in its expression within the continuously regenerating small intestinal epithelium....

Cellular differentiation in the emerging fetal rat small intestinal epithelium: mosaic patterns of gene expression.

Rubin, D C, Ong, D E, Gordon, J I

We have examined the pattern of differentiation of the small intestinal epithelium in fetal rats during the 17th through 21st days of gestation. Five genes expressed in late fetal, neonatal, and...

Altered membrane association of p60v-src and a murine 63-kDa N-myristoyl protein after incorporation of an oxygen-substituted analog of myristic acid.

Heuckeroth, R O, Gordon, J I

A number of viral and cellular proteins contain covalently bound lipid. In a subset of these acyl proteins, the 14-carbon saturated fatty acid myristic acid is attached through an amide linkage to an...

Refined apoprotein structure of rat intestinal fatty acid binding protein produced in Escherichia coli.

Sacchettini, J C, Gordon, J I, Banaszak, L J

Rat intestinal fatty acid binding protein (I-FABP) is a member of a family of cytoplasmic hydrophobic ligand-binding proteins. To gain insights about the contribution of bound fatty acid to I-FABP's...

Replication of human immunodeficiency virus 1 and Moloney murine leukemia virus is inhibited by different heteroatom-containing analogs of myristic acid.

Bryant, M L, Heuckeroth, R O, Kimata, J T, Ratner, L, Gordon, J I

Myristoyl-CoA:protein N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the cotranslational linkage of myristate to the N-terminal glycine residues of several cellular, viral, and oncoproteins. We...

Rat intestinal apolipoprotein B gene expression. Evidence for integrated regulation by bile salt, fatty acid, and phospholipid flux.

Davidson, N O, Drewek, M J, Gordon, J I, Elovson, J

We previously reported that intestinal apo B48 synthesis in the rat was unaltered by dietary triglyceride intake but demonstrated regulation in response to biliary lipid availability. Studies are now...

Globin gene expression in erythroid human fetal liver cells.

Ley, T J, Maloney, K A, Gordon, J I, Schwartz, A L

We measured steady-state levels of the human globin mRNAs in liver samples from several mid-gestational fetuses. RNA from the epsilon, gamma, beta, zeta, theta, and alpha globin genes were present in...

Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase.

Towler, D A, Adams, S P, Eubanks, S R, Towery, D S, Jackson-Machelski, E, Glaser, L, ...

Myristoyl CoA:protein N-myristoyltransferase (NMT) catalyzes the addition of myristic acid to the amino-terminal glycine residues of a number of eukaryotic proteins. Recently, we developed a...

Developmental regulation of a gene that encodes a cysteine-rich intestinal protein and maps near the murine immunoglobulin heavy chain locus.

Birkenmeier, E H, Gordon, J I

Mouse and rat small intestinal cDNA libraries were screened for recombinants derived from mRNAs whose concentration changed during the transition from suckling to weaning. cDNAs transcribed from a...

Cloning of a cDNA encoding rat intestinal fatty acid binding protein.

Alpers, D H, Strauss, A W, Ockner, R K, Bass, N M, Gordon, J I

Intestinal fatty acid binding protein mRNA is one of the most abundant mRNA species in the rat small intestinal epithelium. RNA transfer blot analyses disclosed that the mRNA encoding intestinal...

Coordinate regulation of two estrogen-dependent genes in avian liver.

Wiskocil, R, Bensky, P, Dower, W, Goldberger, R F, Gordon, J I, Deeley, R G

Livers of egg-laying species contain abundant mRNAs encoded by both estrogen-responsive and constitutively expressed genes. We have recently constructed cDNA clones from three members of the abundant...

Rat cellular retinol-binding protein II: use of a cloned cDNA to define its primary structure, tissue-specific expression, and developmental regulation.

Li, E, Demmer, L A, Sweetser, D A, Ong, D E, Gordon, J I

The primary structure of rat cellular retinol-binding protein (CRBP) II has been determined from a cloned cDNA. Alignment of this 134-amino acid, 15,580-Da polypeptide with rat CRBP revealed that 75...

Tissue-specific expression and developmental regulation of the rat apolipoprotein B gene.

Demmer, L A, Levin, M S, Elovson, J, Reuben, M A, Lusis, A J, Gordon, J I

Expression of the apolipoprotein B (apoB) gene was examined in a variety of fetal, neonatal, and adult rat tissues by probing RNA blots with a cloned rat apoB cDNA. Among 10 adult male tissues...

Expression of rat apolipoprotein A-IV and A-I genes: mRNA induction during development and in response to glucocorticoids and insulin.

Elshourbagy, N A, Boguski, M S, Liao, W S, Jefferson, L S, Gordon, J I, Taylor, J M

Rat apolipoproteins (apo-) A-IV and A-I share many structural similarities, the most notable of which is a domain of repeated docosapeptides with amphipathic helical potential. Although the genes for...

Rat apolipoprotein A-IV contains 13 tandem repetitions of a 22-amino acid segment with amphipathic helical potential.

Boguski, M S, Elshourbagy, N, Taylor, J M, Gordon, J I

Apolipoprotein (apo) A-IV is a 46,000 Mr protein that is a major component of rat high density lipoproteins and chylomicrons. We have isolated, from a rat intestinal cDNA library, a full-length apo...

Primary induction of vitellogenin mRNA in the rooster by 17beta-estradiol.

Burns, A T, Deeley, R G, Gordon, J I, Udell, D S, Mullinix, K P, Goldberger, R F

We have studied the kinetics of vitellogenin mRNA accumulation in rooster liver after a primary injection of 17beta-estradiol. The levels of vitellogenin mRNA have been determined both by...

Comparative analysis of repeated sequences in rat apolipoproteins A-I, A-IV, and E.

Boguski, M S, Elshourbagy, N, Taylor, J M, Gordon, J I

To understand the structural, functional, and evolutionary relationships among the principal protein components of rat high density lipoprotein particles, we undertook a systematic comparative...

Comparative in vitro study of the pro-apolipoprotein A-I to apolipoprotein A-I converting activity between normal and Tangier plasma.

Edelstein, C, Gordon, J I, Vergani, C A, Catapano, A L, Pietrini, V, Scanu, A M

We examined the ability of the plasma of a 52-yr-old male Tangier patient to effect the conversion of radiolabeled pro-apolipoprotein A-I (apo A-I), isolated from hepatoma cell culture media, into...

Induction of vitellogenin synthesis by estrogen in avian liver: relationship between level of vitellogenin mRNA and vitellogenin synthesis.

Mullinix, K P, Wetekam, W, Deeley, R G, Gordon, J I, Meyers, M, Kent, K A, ...

We have investigated the estrogen-mediated induction of vitellogenin synthesis in rooster liver. We compared the concentrations of vitellogenin messenger RNA (mRNA) in the liver with the...

Mutations of human myristoyl-CoA:protein N-myristoyltransferase cause temperature-sensitive myristic acid auxotrophy in Saccharomyces cerevisiae.

Duronio, R J, Reed, S I, Gordon, J I

We have isolated cDNAs encoding human myristoyl-CoA:protein N-myristoyltransferase (NMT, EC 2.3.1.97) by complementing the nmtl-181 mutation of Saccharomyces cerevisiae, which causes...