Production of recombinat Conkunitzin-S1 in Escherichia coli. (2006)
Bayrhuber, M., Graf, R., Ferber, M., Zweckstetter, M., Imperial, J., Garrett, J. E., ...
Biodiversity of uptake hydrogenase systems from legume endosymbiotic bacteria (2005)
Brito, B., Baginsky, Cecilia, Palacios, J. M., Cabrera, E., Ruiz-Argüeso, T., Imperial, J.
Uptake hydrogenases in legume endosymbiotic bacteria recycle hydrogen produced during the nitrogen fixation process in legume nodules. Despite the described beneficial effect on plant productivity,...
Brito, B., Palacios, J. M., Imperial, J., Ruiz-Argüeso, T.
Rhizobium leguminosarum bv. viciae UPM791 induces hydrogenase activity in pea (Pisum sativum L.) bacteroids but not in free-living cells. The symbiotic induction of hydrogenase structural genes...
Hernando, Y, Palacios, J M, Imperial, J, Ruiz-Argüeso, T
Pea (Pisum sativum L.) bacteroids produced by Rhizobium leguminosarum bv. viciae UPM791 synthesize a membrane-bound (NiFe) hydrogenase which oxidizes H2 arising from the nitrogen fixation process in...
Gutiérrez, D, Hernando, Y, Palacios, J M, Imperial, J, Ruiz-Argüeso, T
Rhizobium leguminosarum bv. viciae UPM791 contains a second copy of the fnrN gene, which encodes a redox-sensitive transcriptional activator functionally homologous to Escherichia coli Fnr. This...
Brito, B, Palacios, J M, Hidalgo, E, Imperial, J, Ruiz-Argüeso, T
Rhizobium leguminosarum bv. viciae UPM791 induces the synthesis of an [NiFe] hydrogenase in pea (Pisum sativum L.) bacteroids which oxidizes the H2 generated by the nitrogenase complex inside the...
Rey, L, Imperial, J, Palacios, J M, Ruiz-Argüeso, T
The products of the Rhizobium leguminosarum hyp gene cluster are necessary for synthesis of a functional uptake [NiFe] hydrogenase system in symbiosis with pea plants, and at least for HypB and HypF,...
Rodríguez-Quiñones, F, Bosch, R, Imperial, J
The nifBQ transcriptional unit of Azotobacter vinelandii has been previously shown to be required for activity of the three nitrogenase systems, Mo nitrogenase, V nitrogenase, and Fe nitrogenase,...
Homocitrate cures the NifV- phenotype in Klebsiella pneumoniae.
Hoover, T R, Imperial, J, Ludden, P W, Shah, V K
Dinitrogenase was isolated from a culture of a Klebsiella pneumoniae NifV- strain derepressed for nitrogenase in the presence of homocitrate. The enzyme isolated from this culture was identical to...
Iron-molybdenum cofactor synthesis in Azotobacter vinelandii Nif- mutants.
Imperial, J, Shah, V K, Ugalde, R A, Ludden, P W, Brill, W J
Nif- mutants of Azotobacter vinelandii defective in dinitrogenase activity synthesized iron-molybdenum cofactor (FeMo-co) and accumulated it in two protein-bound forms: inactive dinitrogenase and a...
Imperial, J, Ugalde, R A, Shah, V K, Brill, W J
NifQ- mutants of Klebsiella pneumoniae are defective in nitrogen fixation due to an elevated requirement for molybdenum. When millimolar concentrations of molybdate were added to the medium, the...
Biosynthesis of iron-molybdenum cofactor in the absence of nitrogenase.
Ugalde, R A, Imperial, J, Shah, V K, Brill, W J
Klebsiella pneumoniae accumulates molybdenum during nitrogenase derepression. The molybdenum is primarily in nitrogenase component I in the form of iron-molybdenum cofactor (FeMo-co). Mutations in...
Mol- mutants of Klebsiella pneumoniae requiring high levels of molybdate for nitrogenase activity.
Imperial, J, Ugalde, R A, Shah, V K, Brill, W J
Mol- mutants of Klebsiella pneumoniae requiring high levels of molybdate for nitrogenase and nitrate reductase activity were characterized. The effects of mol mutations on nitrogenase activity were...
Ugalde, R A, Imperial, J, Shah, V K, Brill, W J
NifQ- and Mol- mutants of Klebsiella pneumoniae show an elevated molybdenum requirement for nitrogen fixation. Substitution of cystine for sulfate as the sulfur source in the medium reduced the...
In vitro synthesis of the iron-molybdenum cofactor of nitrogenase.
Shah, V K, Imperial, J, Ugalde, R A, Ludden, P W, Brill, W J
Molybdate- and ATP-dependent in vitro synthesis of the iron-molybdenum cofactor (FeMo-co) of nitrogenase requires the protein products of at least the nifB, nifN, and nifE genes. Extracts of...
Brito, B., Palacios, J. M., Imperial, J., Ruiz-Argüeso, T.
Rhizobium leguminosarum bv. viciae UPM791 induces hydrogenase activity in pea (Pisum sativum L.) bacteroids but not in free-living cells. The symbiotic induction of hydrogenase structural genes...
Hernando, Y, Palacios, J M, Imperial, J, Ruiz-Argüeso, T
Pea (Pisum sativum L.) bacteroids produced by Rhizobium leguminosarum bv. viciae UPM791 synthesize a membrane-bound (NiFe) hydrogenase which oxidizes H2 arising from the nitrogen fixation process in...
Gutiérrez, D, Hernando, Y, Palacios, J M, Imperial, J, Ruiz-Argüeso, T
Rhizobium leguminosarum bv. viciae UPM791 contains a second copy of the fnrN gene, which encodes a redox-sensitive transcriptional activator functionally homologous to Escherichia coli Fnr. This...
Brito, B, Palacios, J M, Hidalgo, E, Imperial, J, Ruiz-Argüeso, T
Rhizobium leguminosarum bv. viciae UPM791 induces the synthesis of an [NiFe] hydrogenase in pea (Pisum sativum L.) bacteroids which oxidizes the H2 generated by the nitrogenase complex inside the...
Rey, L, Imperial, J, Palacios, J M, Ruiz-Argüeso, T
The products of the Rhizobium leguminosarum hyp gene cluster are necessary for synthesis of a functional uptake [NiFe] hydrogenase system in symbiosis with pea plants, and at least for HypB and HypF,...
Rodríguez-Quiñones, F, Bosch, R, Imperial, J
The nifBQ transcriptional unit of Azotobacter vinelandii has been previously shown to be required for activity of the three nitrogenase systems, Mo nitrogenase, V nitrogenase, and Fe nitrogenase,...
Homocitrate cures the NifV- phenotype in Klebsiella pneumoniae.
Hoover, T R, Imperial, J, Ludden, P W, Shah, V K
Dinitrogenase was isolated from a culture of a Klebsiella pneumoniae NifV- strain derepressed for nitrogenase in the presence of homocitrate. The enzyme isolated from this culture was identical to...
Iron-molybdenum cofactor synthesis in Azotobacter vinelandii Nif- mutants.
Imperial, J, Shah, V K, Ugalde, R A, Ludden, P W, Brill, W J
Nif- mutants of Azotobacter vinelandii defective in dinitrogenase activity synthesized iron-molybdenum cofactor (FeMo-co) and accumulated it in two protein-bound forms: inactive dinitrogenase and a...
Imperial, J, Ugalde, R A, Shah, V K, Brill, W J
NifQ- mutants of Klebsiella pneumoniae are defective in nitrogen fixation due to an elevated requirement for molybdenum. When millimolar concentrations of molybdate were added to the medium, the...
Biosynthesis of iron-molybdenum cofactor in the absence of nitrogenase.
Ugalde, R A, Imperial, J, Shah, V K, Brill, W J
Klebsiella pneumoniae accumulates molybdenum during nitrogenase derepression. The molybdenum is primarily in nitrogenase component I in the form of iron-molybdenum cofactor (FeMo-co). Mutations in...
Mol- mutants of Klebsiella pneumoniae requiring high levels of molybdate for nitrogenase activity.
Imperial, J, Ugalde, R A, Shah, V K, Brill, W J
Mol- mutants of Klebsiella pneumoniae requiring high levels of molybdate for nitrogenase and nitrate reductase activity were characterized. The effects of mol mutations on nitrogenase activity were...
Ugalde, R A, Imperial, J, Shah, V K, Brill, W J
NifQ- and Mol- mutants of Klebsiella pneumoniae show an elevated molybdenum requirement for nitrogen fixation. Substitution of cystine for sulfate as the sulfur source in the medium reduced the...
In vitro synthesis of the iron-molybdenum cofactor of nitrogenase.
Shah, V K, Imperial, J, Ugalde, R A, Ludden, P W, Brill, W J
Molybdate- and ATP-dependent in vitro synthesis of the iron-molybdenum cofactor (FeMo-co) of nitrogenase requires the protein products of at least the nifB, nifN, and nifE genes. Extracts of...