Wilkinson, R J, Hasløv, K, Rappuoli, R, Giovannoni, F, Narayanan, P R, Desai, C R, ...
The diagnosis of infection caused by Mycobacterium tuberculosis is of increased public health concern following increases in the number of cases in developed countries and major increases in...
Häcker, G, Kromer, S, Heeg, K, Ivanyi, J, Wagner, H, Pfeffer, K
Human gamma delta T cells are known to respond at high frequencies to pathogenic mycobacteria. Here we show that opportunistic strains of mycobacteria share with pathogenic mycobacteria the ability...
Immunological activity of a 38-kilodalton protein purified from Mycobacterium tuberculosis.
Young, D, Kent, L, Rees, A, Lamb, J, Ivanyi, J
A 38-kilodalton (kDa) protein antigen from Mycobacterium tuberculosis was purified by monoclonal antibody TB71-based affinity chromatography. This molecule carries two nonoverlapping epitopes...
Characterization of antibody-reactive epitopes on the 65-kilodalton protein of Mycobacterium leprae.
Buchanan, T M, Nomaguchi, H, Anderson, D C, Young, R A, Gillis, T P, Britton, W J, ...
Twenty-three monoclonal antibodies (MAbs) prepared in seven different laboratories were studied, all of which recognized the 65-kilodalton (kDa) protein of Mycobacterium leprae as determined by...
Identification of a 68-kilodalton protective protein antigen from Bordetella bronchiseptica.
Montaraz, J A, Novotny, P, Ivanyi, J
A 68-kilodalton (kd) outer membrane protein antigen of Bordetella bronchiseptica has been identified by using monoclonal antibodies that recognized two nonoverlapping determinants. Antibody BB05 also...
Specificity of antibodies to immunodominant mycobacterial antigens in pulmonary tuberculosis.
Jackett, P S, Bothamley, G H, Batra, H V, Mistry, A, Young, D B, Ivanyi, J
A serological survey was performed in groups of patients with active sputum smear-positive or smear-negative pulmonary tuberculosis, healthy household contacts, and controls. Sera were tested for...
Levels of antibody to defined antigens of Mycobacterium tuberculosis in tuberculous meningitis.
Chandramuki, A, Bothamley, G H, Brennan, P J, Ivanyi, J
The aim of the study was to develop an antibody immunoassay for tuberculous meningitis with a sensitivity greater than that of the initial bacterial smear while maintaining a 100% specificity....
Garbe, T, Harris, D, Vordermeier, M, Lathigra, R, Ivanyi, J, Young, D
The gene encoding a 19-kDa antigen from Mycobacterium tuberculosis was expressed as a recombinant protein in the rapid-growing species Mycobacterium smegmatis. The recombinant antigen was expressed...
Epitope specificity and isoforms of the mycobacterial 19-kilodalton antigen.
Harris, D P, Vordermeier, H M, Brett, S J, Pasvol, G, Moreno, C, Ivanyi, J
The topography and specificity of B- and T-cell stimulatory epitopes from the 19-kDa protein of Mycobacterium tuberculosis were investigated by using overlapping synthetic peptides. Murine antisera...
Mechanisms of recrudescence of Mycobacterium bovis BCG infection in mice.
Cox, J H, Knight, B C, Ivanyi, J
The capacity of various immunosuppressive agents to cause a recrudescence of the replication of Mycobacterium bovis BCG in the spleens of chronically infected mice was investigated. The actions of...
Dissection of Mycobacterium tuberculosis antigens using recombinant DNA.
Young, R A, Bloom, B R, Grosskinsky, C M, Ivanyi, J, Thomas, D, Davis, R W
A recombinant DNA strategy has been used systematically to survey the Mycobacterium tuberculosis genome for sequences that encode specific antigens detected by monoclonal antibodies. M. tuberculosis...
Mapping of T cell epitopes using recombinant antigens and synthetic peptides.
Lamb, J R, Ivanyi, J, Rees, A D, Rothbard, J B, Howland, K, Young, R A, ...
Two complementary approaches were used to determine the epitope specificity of clonal and polyclonal human T lymphocytes reactive with the 65-kd antigen of Mycobacterium leprae. A recombinant DNA...
Antigenic, receptor-binding and mitogenic activity of proteolytic fragments of human growth hormone.
Analysis of the subtilisin-digested, two-chain form of human growth hormone (hGH) and its constituent polypeptide fragments has been aided by the use of monoclonal antibodies which bind specifically...
Wilkinson, R J, Hasløv, K, Rappuoli, R, Giovannoni, F, Narayanan, P R, Desai, C R, ...
The diagnosis of infection caused by Mycobacterium tuberculosis is of increased public health concern following increases in the number of cases in developed countries and major increases in...
Häcker, G, Kromer, S, Heeg, K, Ivanyi, J, Wagner, H, Pfeffer, K
Human gamma delta T cells are known to respond at high frequencies to pathogenic mycobacteria. Here we show that opportunistic strains of mycobacteria share with pathogenic mycobacteria the ability...
Immunological activity of a 38-kilodalton protein purified from Mycobacterium tuberculosis.
Young, D, Kent, L, Rees, A, Lamb, J, Ivanyi, J
A 38-kilodalton (kDa) protein antigen from Mycobacterium tuberculosis was purified by monoclonal antibody TB71-based affinity chromatography. This molecule carries two nonoverlapping epitopes...
Characterization of antibody-reactive epitopes on the 65-kilodalton protein of Mycobacterium leprae.
Buchanan, T M, Nomaguchi, H, Anderson, D C, Young, R A, Gillis, T P, Britton, W J, ...
Twenty-three monoclonal antibodies (MAbs) prepared in seven different laboratories were studied, all of which recognized the 65-kilodalton (kDa) protein of Mycobacterium leprae as determined by...
Identification of a 68-kilodalton protective protein antigen from Bordetella bronchiseptica.
Montaraz, J A, Novotny, P, Ivanyi, J
A 68-kilodalton (kd) outer membrane protein antigen of Bordetella bronchiseptica has been identified by using monoclonal antibodies that recognized two nonoverlapping determinants. Antibody BB05 also...
Specificity of antibodies to immunodominant mycobacterial antigens in pulmonary tuberculosis.
Jackett, P S, Bothamley, G H, Batra, H V, Mistry, A, Young, D B, Ivanyi, J
A serological survey was performed in groups of patients with active sputum smear-positive or smear-negative pulmonary tuberculosis, healthy household contacts, and controls. Sera were tested for...
Levels of antibody to defined antigens of Mycobacterium tuberculosis in tuberculous meningitis.
Chandramuki, A, Bothamley, G H, Brennan, P J, Ivanyi, J
The aim of the study was to develop an antibody immunoassay for tuberculous meningitis with a sensitivity greater than that of the initial bacterial smear while maintaining a 100% specificity....
Garbe, T, Harris, D, Vordermeier, M, Lathigra, R, Ivanyi, J, Young, D
The gene encoding a 19-kDa antigen from Mycobacterium tuberculosis was expressed as a recombinant protein in the rapid-growing species Mycobacterium smegmatis. The recombinant antigen was expressed...
Epitope specificity and isoforms of the mycobacterial 19-kilodalton antigen.
Harris, D P, Vordermeier, H M, Brett, S J, Pasvol, G, Moreno, C, Ivanyi, J
The topography and specificity of B- and T-cell stimulatory epitopes from the 19-kDa protein of Mycobacterium tuberculosis were investigated by using overlapping synthetic peptides. Murine antisera...
Mechanisms of recrudescence of Mycobacterium bovis BCG infection in mice.
Cox, J. H., Knight, B. C., Ivanyi, J.
The capacity of various immunosuppressive agents to cause a recrudescence of the replication of Mycobacterium bovis BCG in the spleens of chronically infected mice was investigated. The actions of...
Dissection of Mycobacterium tuberculosis antigens using recombinant DNA.
Young, R A, Bloom, B R, Grosskinsky, C M, Ivanyi, J, Thomas, D, Davis, R W
A recombinant DNA strategy has been used systematically to survey the Mycobacterium tuberculosis genome for sequences that encode specific antigens detected by monoclonal antibodies. M. tuberculosis...
Mapping of T cell epitopes using recombinant antigens and synthetic peptides.
Lamb, J R, Ivanyi, J, Rees, A D, Rothbard, J B, Howland, K, Young, R A, ...
Two complementary approaches were used to determine the epitope specificity of clonal and polyclonal human T lymphocytes reactive with the 65-kd antigen of Mycobacterium leprae. A recombinant DNA...
Antigenic, receptor-binding and mitogenic activity of proteolytic fragments of human growth hormone.
Analysis of the subtilisin-digested, two-chain form of human growth hormone (hGH) and its constituent polypeptide fragments has been aided by the use of monoclonal antibodies which bind specifically...
Zhu, X, Venkataprasad, N, Ivanyi, J, Vordermeier, H M
A number of subunit-based vaccine candidates have recently begun to erode the exclusive position of Mycobacterium bovis bacillus Calmette-Guérin (BCG), which gives unpredictable and highly variable...
Chua-Intra, B, Ivanyi, J, Hills, A, Thole, J, Moreno, C, Vordermeier, H M
The Mycobacterium leprae and M. tuberculosis 10,000 MW heat-shock protein homologues of GroES have previously been identified as major immunogens for human T cells. We used synthetic peptides to...
Wilkinson, R J, Zhu, X, Wilkinson, K A, Lalvani, A, Ivanyi, J, Pasvol, G, ...
CD8+ T lymphocytes are required to protect mice against Mycobacterium tuberculosis, although in early infection the mechanism appears not to be via perforin or granzyme-mediated lysis of the infected...
Román, E, Harris, D P, Jurcevic, S, Ivanyi, J, Moreno, C
Previously we have identified an immunodominant, eight-residue, epitope core sequence (TAAGNVNI) from the 19,000 MW protein of Mycobacterium tuberculosis, which is recognized in the context of...
Thomssen, H, Ivanyi, J, Espitia, C, Arya, A, Londei, M
Double-negative alpha beta+ T-cell receptor (TCR) human T cells have been reported to recognize antigen in the context of the HLA class I-like (Ib) CD1 complex. In particular, the CD1b molecule has...
Genetic influences on the immune repertoire following tuberculous infection in mice.
Differences in the antibody repertoire between B10 and BALB background strains have been found following intraperitoneal infection of mice with Mycobacterium tuberculosis. Western blot analysis...
Faith, A, Moreno, C, Lathigra, R, Roman, E, Fernandez, M, Brett, S, ...
The potential number of T-cell epitopes in the 19,000 molecular weight (MW) antigen has been investigated using overlapping peptides which comprise the complete sequence. Sixteen potential epitopes...
Cox, J H, Lamb, J R, Bal, V, Butcher, G W, Howard, J C, Owen, M J, ...
The Nb2 rat lymphoma cell line has the unique property that its growth is dependent on lactogenic pituitary hormones. Cell surface staining with monoclonal antibodies showed expression of class I MHC...
Bursal origin of bone marrow cells with competence for antibody formation
Ivanyi, J., Murgatroyd, L. B., Lydyard, P. M.
The impaired antibody response of neonatally bursectomized chickens was restored by bone marrow cells from normal—but not from bursectomized—syngeneic donors. Chimaerism of donor cells in the...
Cellular mechanisms of escape from immunological tolerance
Immunological tolerance to human serum albumin (HSA) was induced in chickens by large doses of antigen injected post-hatching. Bursectomy or thymectomy performed at the age of 2 weeks did not affect...
Harris, D P, Vordermeier, H M, Arya, A, Moreno, C, Ivanyi, J
Most T-cell epitopes are recognized in the context of a single or limited number of major histocompatibility complex (MHC) class II molecules. We have shown previously, however, that the...
Elsaghier, A, Nolan, A, Allen, B, Ivanyi, J
Systemic infection of mice with organisms of the Mycobacterium avium complex (MAC) induced antibody responses, characteristic for each of the three tested individual strains. The influence of host...
Brett, S, Orrell, J M, Swanson Beck, J, Ivanyi, J
Mice infected by intraperitoneal injection of Mycobacterium tuberculosis were studied over a 23-week period. They showed progressive infection in the lung (with increasing microbial count and...
Vordermeier, H M, Harris, D P, Lathigra, R, Roman, E, Moreno, C, Ivanyi, J
The T-cell repertoire to a prominent immunogen of Mycobacterium tuberculosis has been investigated on the assumption that differences in epitope specificity could influence the protective and...
Rees, A D, Faith, A, Roman, E, Ivanyi, J, Wiesmuller, K H, Moreno, C
The mechanisms contributing to the dominant and degenerate recognition of the N-terminal region of the Mycobacterium tuberculosis 19,000 MW protein have been investigated. Using polyclonal and cloned...
Surcel, H M, Troye-Blomberg, M, Paulie, S, Andersson, G, Moreno, C, Pasvol, G, ...
Proliferation and cytokine production profiles by blood mononuclear cells in response to in vitro stimulation with mycobacterial antigens were compared in patients with active tuberculosis and in...
Kobayashi, N, Matsuzaki, G, Yoshikai, Y, Seki, R, Ivanyi, J, Nomoto, K
The purpose of this paper was to study the gamma delta T-cell receptor repertoire and target specificity following stimulation of peripheral T cells of BALB/c mice with autologous or bacterial...
Developed γM haemolytic plaque-forming cells in chickens
Incorporation of rabbit anti-μ serum into agarose gels inhibited direct anti-SRBC PFCs using chicken complement, but developed equal numbers of indirect PFCs binding guinea-pig complement. The...
Kierszenbaum, F, Ivanyi, J, Budzko, D B
The natural resistance of chickens to Trypanosoma curzi infection and the capacity of their sera to lyse blood (trypomastigote) forms of the parasite in vitro were found to be complement-dependent...
Chickens were treated at an early embryonal age with testosterone propionate or infected neonatally with the virus producing Gumboro disease. Their sera were subsequently fractionated by Sephadex...
Intravenous injection of LPS or allogeneic lymphocytes resulted in impaired maturation of B cells within the bursa of chick embryos. A gradual decline in the sensitivity of embryos towards both...
The extent of suppression of antibody response by bursectomy (Bx) was examined as a measure of the seeding sequence of different clones from the bursa to peripheral lymphoid tissues. Chickens were...
Control by H-2 genes of murine antibody responses to protein antigens of Mycobacterium tuberculosis.
The genetic control of antibody responses after immunization with Mycobacterium tuberculosis soluble antigens was examined in inbred and H-2 congenic mouse strains. Antibody levels to five distinct...
T-cell activation by anti-idiotypic antibody: evidence for the internal image.
Rees, A D, Praputpittaya, K, Scoging, A, Dobson, N, Ivanyi, J, Young, D, ...
Human lymphoproliferative responses to a rabbit anti-idiotypic antibody (anti-Id TB71) and the corresponding mycobacterial protein antigen [38,000 molecular weight (MW)] have been investigated in a...
Immunogenicity of peptides for B cells is not impaired by overlapping T-cell epitope topology.
Harris, D P, Vordermeier, H M, Arya, A, Bogdan, K, Moreno, C, Ivanyi, J
The epitope specificity of T-cell help to B cells and of surface immunoglobulin-mediated B-cell-binding of antigens usually involves topographically distinct antigenic determinants. The possibility...
Allelic exclusion of M1 (IgM) allotype on the surface of chicken B cells.
The membrane expression of M1 (IgM) and G1 (IgG) allotype markers on peripheral blood lymphocytes was examined by immunofluorescence. In homozygous chickens 15% lymphocytes stained with either...
We have analysed the nature of rosette forming cells (RFC) in peripheral blood lymphocytes (PBL) from chickens which had been immunized with SRBC 7 days previously. Although none of the RFC secreted...
Sarmay, G., Sanderson, A., Ivanyi, J.
The association between Fc receptors and other surface molecules was examined by EA-rosette (EAR) inhibition experiments. Twenty to 30% EAR were detected in suspensions of peripheral blood...
Evaluation of a monoclonal antibody (TB72) based serological test for tuberculosis.
Ivanyi, J, Krambovitis, E, Keen, M
A serological test for tuberculosis (TB), based on competitive inhibition by human sera of antigen binding by a murine monoclonal antibody (TB72) has been performed using solid phase...
Ivanyi, J, Sinha, S, Aston, R, Cussell, D, Keen, M, Sengupta, U
Four soluble antigens of Mycobacterium leprae have been identified using 12 murine monoclonal antibodies. Their specificity, taxonomic distribution and molecular nature were analysed by...
Immunodeficiency in the chicken. IV. An immunological study of infectious bursal disease.
Chickens inoculated orally with infectious bursal disease virus (IBDV) 1 day after hatching subsequently showed a 50% incidence of immunodeficiency but little mortality. Antibody responses against...
Rabbit antisera were raised against four monoclonal antibodies (MoAb) binding with the 35 kD protein and four MoAb binding with the 12 kD protein antigen of Mycobacterium leprae. Antisera showed...
Rabbit antisera raised against eight monoclonal antibodies (MoAb) binding to distinct mycobacterial antigens revealed individual anti-idiotype specificities following cross-absorption with normal...
Sengupta, U, Sinha, S, Ramu, G, Lamb, J, Ivanyi, J
Delayed hypersensitivity skin reactions to tuberculin when injected alone or in mixture with antigens of M. leprae were examined in leprosy patients and in healthy controls. The tuberculin reaction...
Human T cell responses to peptide epitopes of the 16-kD antigen in tuberculosis.
Friscia, G, Vordermeier, H M, Pasvol, G, Harris, D P, Moreno, C, Ivanyi, J
The 16-kD protein constituent of the Mycobacterium tuberculosis complex has been known mainly for its prominent serological immunogenicity and species specificity in tuberculous infection. In this...
Antibodies to Mycobacterium tuberculosis-specific epitopes in lepromatous leprosy.
Bothamley, G, Beck, J S, Britton, W, Elsaghier, A, Ivanyi, J
Sera from patients with leprosy or tuberculosis and healthy subjects have been analysed for the presence of antibodies to four species-specific mycobacterial epitopes, four different viruses and five...
Disease association of antibodies to human and mycobacterial hsp70 and hsp60 stress proteins.
Elsaghier, A, Prantera, C, Bothamley, G, Wilkins, E, Jindal, S, Ivanyi, J
Structural homology between microbial and human stress proteins has been postulated to be a basis for autoimmunization in chronic inflammatory diseases. Therefore, we estimated by ELISA titration the...
Antibodies to Mycobacterium paratuberculosis-specific protein antigens in Crohn's disease.
Elsaghier, A, Prantera, C, Moreno, C, Ivanyi, J
The possible role of infection with Mycobacterium paratuberculosis (MAP) for the etiopathogenesis of Crohn's disease (CD) has been a matter of long-term controversy. In addition to similarities with...
Antigen-dependent in vitro culture of protective T cells from BCG-primed mice.
Induction of protective immunity against pathogenic mycobacteria depends on vaccination with live organisms such as Bacille Calmette-Guérin (BCG). However, it is not known how many and which...
Replica analysis of the class of antibodies produced by single cells
Improvements have been made in a localized haemolysis in gel replica assay developed previously. The technique is designed to show if an individual antibody producing cell releases antibody of one or...
CACCAMO, N, BARERA, A, DI SANO, C, MERAVIGLIA, S, IVANYI, J, HUDECZ, F, ...
The identification of immunodominant and universal mycobacterial peptides could be applied to vaccine design and have an employment as diagnostic reagents. In this paper we have investigated the fine...
TREE, J A, WILLIAMS, A, CLARK, S, HALL, G, MARSH, P D, IVANYI, J
Intranasal vaccination may offer practical benefits and better protection against respiratory infections, including tuberculosis. In this paper, we investigated the persistence of the Mycobacterium...