Dissimilatory Reduction of Fe(III) and Other Electron Acceptors by a Thermus Isolate
Kieft, T. L., Fredrickson, J. K., Onstott, T. C., Gorby, Y. A., Kostandarithes, H. M., Bailey, T. J., ...
A thermophilic bacterium that can use O2, NO3−, Fe(III), and S0 as terminal electron acceptors for growth was isolated from groundwater sampled at a 3.2-km depth in a South African gold mine. This...
Reduction of Fe(III), Cr(VI), U(VI), and Tc(VII) by Deinococcus radiodurans R1
Fredrickson, J. K., Kostandarithes, H. M., Li, S. W., Plymale, A. E., Daly, M. J.
Deinococcus radiodurans is an exceptionally radiation-resistant microorganism capable of surviving acute exposures to ionizing radiation doses of 15,000 Gy and previously described as having a...
Wildung, R. E., Gorby, Y. A., Krupka, K. M., Hess, N. J., Li, S. W., Plymale, A. E., ...
To help provide a fundamental basis for use of microbial dissimilatory reduction processes in separating or immobilizing 99Tc in waste or groundwaters, the effects of electron donor and the presence...
Isolation and characterization of RNA from low-biomass deep-subsurface sediments.
Ogram, A, Sun, W, Brockman, F J, Fredrickson, J K
Three methods for the isolation of microbial RNA from low-biomass deep-subsurface sediments have been developed and evaluated. RNA was isolated from samples taken from depths ranging from 173 to 217...
Aromatic-degrading Sphingomonas isolates from the deep subsurface.
Fredrickson, J K, Balkwill, D L, Drake, G R, Romine, M F, Ringelberg, D B, White, D C
An obligately aerobic chemoheterotrophic bacterium (strain F199) previously isolated from Southeast Coastal Plain subsurface sediments and shown to degrade toluene, naphthalene, and other aromatic...
Fry, N K, Fredrickson, J K, Fishbain, S, Wagner, M, Stahl, D A
Microbial communities of two deep (1,270 and 316 m) alkaline (pH 9.94 and 8.05), anaerobic (Eh, -137 and -27 mV) aquifers were characterized by rRNA-based analyses. Both aquifers, the Grande Ronde...
Stillwell, L C, Thurston, S J, Schneider, R P, Romine, M F, Fredrickson, J K, Saffer, J D
A supercoiled 180-kb plasmid, pNL1, has been isolated from the deep-subsurface, chemoheterotrophic Sphingomonas sp. strain F199, and a physical map was generated. Analysis of a pNL1-derived cosmid...
Ni, S, Fredrickson, J K, Xun, L
Although reductive dehalogenation by anaerobic microorganisms offers great potential for the degradation of halocarbons, little is known about the biochemical mechanisms involved. It has previously...
Fredrickson, J. K., Brockman, F. J., Workman, D. J., Li, S. W., Stevens, T. O.
A bacterium, designated F199, utilized toluene, naphthalene, dibenzothiophene, salicylate, benzoate, p-cresol, and all isomers of xylene as a sole carbon and energy source. This bacterium was...
Bentjen, S. A., Fredrickson, J. K., Van Voris, P., Li, S. W.
Intact soil-core microcosms were studied to determine their applicability for evaluating the transport, survival, and potential ecosystem effects of genetically engineered microorganisms before they...
Isolation and characterization of quinoline-degrading bacteria from subsurface sediments.
Brockman, F J, Denovan, B A, Hicks, R J, Fredrickson, J K
Two gram-negative, motile bacteria isolated from deep subsurface sediments mineralized the nitrogen-containing polyaromatic hydrocarbon quinoline under aerobic conditions and transformed quinoline to...
Balkwill, D. L., Fredrickson, J. K., Thomas, J. M.
Aerobic chemoheterotrophic bacteria were isolated from surface soils and coastal plain subsurface (including deep aquifer) sediments (depths to 265 m) at a study site near Aiken, S.C., by plating on...
Crawford, D L, Doyle, J D, Wang, Z, Hendricks, C W, Bentjen, S A, Bolton, H, ...
A recombinant actinomycete, Streptomyces lividans TK23.1, expressing a pIJ702-encoded extracellular lignin peroxidase gene cloned from the chromosome of Streptomyces viridosporus T7A, was released...
Fredrickson, J K, Bezdicek, D F, Brockman, F J, Li, S W
Investigations were made into the utility of DNA hybridization in conjunction with a microdilution most-probable-number procedure for the enumeration of Rhizobium spp. and Pseudomonas putida in soil....
Plasmid Incidence in Bacteria from Deep Subsurface Sediments
Fredrickson, J. K., Hicks, R. J., Li, S. W., Brockman, F. J.
Bacteria were isolated from deep terrestrial subsurface sediments underlying the coastal plain of South Carolina. A total of 163 isolates from deep sediments, surface soil, and return drill muds were...
Beliaev, A. S., Klingeman, D. M., Klappenbach, J. A., Wu, L., Romine, M. F., Tiedje, J. M., ...
To gain insight into the complex structure of the energy-generating networks in the dissimilatory metal reducer Shewanella oneidensis MR-1, global mRNA patterns were examined in cells exposed to a...
Kieft, T. L., Fredrickson, J. K., McKinley, J. P., Bjornstad, B. N., Rawson, S. A., Phelps, T. J., ...
Twenty-six subsurface samples were collected from a borehole at depths of 173.3 to 196.8 m in the saturated zone at the Hanford Site in south-central Washington State. The sampling was performed...
Dissimilatory Reduction of Fe(III) and Other Electron Acceptors by a Thermus Isolate
Kieft, T. L., Fredrickson, J. K., Onstott, T. C., Gorby, Y. A., Kostandarithes, H. M., Bailey, T. J., ...
A thermophilic bacterium that can use O2, NO3−, Fe(III), and S0 as terminal electron acceptors for growth was isolated from groundwater sampled at a 3.2-km depth in a South African gold mine. This...
Reduction of Fe(III), Cr(VI), U(VI), and Tc(VII) by Deinococcus radiodurans R1
Fredrickson, J. K., Kostandarithes, H. M., Li, S. W., Plymale, A. E., Daly, M. J.
Deinococcus radiodurans is an exceptionally radiation-resistant microorganism capable of surviving acute exposures to ionizing radiation doses of 15,000 Gy and previously described as having a...
Wildung, R. E., Gorby, Y. A., Krupka, K. M., Hess, N. J., Li, S. W., Plymale, A. E., ...
To help provide a fundamental basis for use of microbial dissimilatory reduction processes in separating or immobilizing 99Tc in waste or groundwaters, the effects of electron donor and the presence...
Isolation and characterization of RNA from low-biomass deep-subsurface sediments.
Ogram, A, Sun, W, Brockman, F J, Fredrickson, J K
Three methods for the isolation of microbial RNA from low-biomass deep-subsurface sediments have been developed and evaluated. RNA was isolated from samples taken from depths ranging from 173 to 217...
Aromatic-degrading Sphingomonas isolates from the deep subsurface.
Fredrickson, J K, Balkwill, D L, Drake, G R, Romine, M F, Ringelberg, D B, White, D C
An obligately aerobic chemoheterotrophic bacterium (strain F199) previously isolated from Southeast Coastal Plain subsurface sediments and shown to degrade toluene, naphthalene, and other aromatic...
Fry, N K, Fredrickson, J K, Fishbain, S, Wagner, M, Stahl, D A
Microbial communities of two deep (1,270 and 316 m) alkaline (pH 9.94 and 8.05), anaerobic (Eh, -137 and -27 mV) aquifers were characterized by rRNA-based analyses. Both aquifers, the Grande Ronde...
Stillwell, L C, Thurston, S J, Schneider, R P, Romine, M F, Fredrickson, J K, Saffer, J D
A supercoiled 180-kb plasmid, pNL1, has been isolated from the deep-subsurface, chemoheterotrophic Sphingomonas sp. strain F199, and a physical map was generated. Analysis of a pNL1-derived cosmid...
Ni, S, Fredrickson, J K, Xun, L
Although reductive dehalogenation by anaerobic microorganisms offers great potential for the degradation of halocarbons, little is known about the biochemical mechanisms involved. It has previously...
Fredrickson, J. K., Brockman, F. J., Workman, D. J., Li, S. W., Stevens, T. O.
A bacterium, designated F199, utilized toluene, naphthalene, dibenzothiophene, salicylate, benzoate, p-cresol, and all isomers of xylene as a sole carbon and energy source. This bacterium was...
Bentjen, S. A., Fredrickson, J. K., Van Voris, P., Li, S. W.
Intact soil-core microcosms were studied to determine their applicability for evaluating the transport, survival, and potential ecosystem effects of genetically engineered microorganisms before they...
Isolation and characterization of quinoline-degrading bacteria from subsurface sediments.
Brockman, F J, Denovan, B A, Hicks, R J, Fredrickson, J K
Two gram-negative, motile bacteria isolated from deep subsurface sediments mineralized the nitrogen-containing polyaromatic hydrocarbon quinoline under aerobic conditions and transformed quinoline to...
Balkwill, D. L., Fredrickson, J. K., Thomas, J. M.
Aerobic chemoheterotrophic bacteria were isolated from surface soils and coastal plain subsurface (including deep aquifer) sediments (depths to 265 m) at a study site near Aiken, S.C., by plating on...
Crawford, D L, Doyle, J D, Wang, Z, Hendricks, C W, Bentjen, S A, Bolton, H, ...
A recombinant actinomycete, Streptomyces lividans TK23.1, expressing a pIJ702-encoded extracellular lignin peroxidase gene cloned from the chromosome of Streptomyces viridosporus T7A, was released...
Fredrickson, J K, Bezdicek, D F, Brockman, F J, Li, S W
Investigations were made into the utility of DNA hybridization in conjunction with a microdilution most-probable-number procedure for the enumeration of Rhizobium spp. and Pseudomonas putida in soil....
Plasmid Incidence in Bacteria from Deep Subsurface Sediments
Fredrickson, J. K., Hicks, R. J., Li, S. W., Brockman, F. J.
Bacteria were isolated from deep terrestrial subsurface sediments underlying the coastal plain of South Carolina. A total of 163 isolates from deep sediments, surface soil, and return drill muds were...
Beliaev, A. S., Klingeman, D. M., Klappenbach, J. A., Wu, L., Romine, M. F., Tiedje, J. M., ...
To gain insight into the complex structure of the energy-generating networks in the dissimilatory metal reducer Shewanella oneidensis MR-1, global mRNA patterns were examined in cells exposed to a...
Kieft, T. L., Fredrickson, J. K., McKinley, J. P., Bjornstad, B. N., Rawson, S. A., Phelps, T. J., ...
Twenty-six subsurface samples were collected from a borehole at depths of 173.3 to 196.8 m in the saturated zone at the Hanford Site in south-central Washington State. The sampling was performed...