Inhibition of osteocyte apoptosis by fluid flow is mediated by nitric oxide (2008)
Tan, S.D., Bakker, A.D., Semeins, C.M., Kuijpers-Jagtman, A.M., Klein-Nulend, J.
Bone unloading results in osteocyte apoptosis, which attracts osteoclasts leading to bone loss. Loading of bone drives fluid flow over osteocytes which respond by releasing signaling molecules, like...
Osteocyte morphology in fibula and calvaria - Is there a role for mechanosensing? (2008)
Vatsa, A., Breuls, R.G., Semeins, C.M., Salmon, P.L., Smit, T.H., Klein-Nulend, J.
Introduction External mechanical forces on cells are known to influence cytoskeletal structure and thus cell shape. Mechanical loading in long bones is unidirectional along their long axes, whereas...
Paxillin localisation in osteocytes – Is it determined by the direction of loading? (2008)
Vatsa, A., Semeins, C.M., Smit, T.H., Klein-Nulend, J.
External mechanical loading of cells aligns cytoskeletal stress fibres in the direction of principle strains and localises paxillin to the mechanosensing region. If the osteocyte cell body can indeed...
The polyamine spermine regulates osteogenic differentiation in adipose stem cells (2008)
Tjabringa, G.S., Zandieh-Doulabi, B., Helder, M.N., Knippenberg, M., Klein-Nulend, J.
For bone tissue engineering, it is important that mesenchymal stem cells (MSCs) differentiate into osteoblasts. To develop a method for differentiation of adipose tissue-derived mesenchymal stem...
Low-intensity pulsed ultrasound affects human articular chondrocytes in vitro (2008)
Korstjens, C.M., Albers, G.H., Semeins, C.M., Klein-Nulend, J.
We investigated whether low-intensity pulsed ultrasound (LIPUS) stimulates chondrocyte proliferation and matrix production in explants of human articular cartilage obtained from donors suffering from...