A fast, very simple and efficient method of DNA extraction is described which takes advantage of DNA sedimentation induced by millimolar concentrations of ZnCl2. The zinc-induced sedimentation is...
Conformational properties of DNA strands containing guanine-adenine and thymine-adenine repeats.
Vorlicková, M, Kejnovská, I, Kovanda, J, Kypr, J
CD spectroscopy and PAGE were used to cooperatively analyze melting conformers of DNA strands containing GA and TA dinucleotide repeats. The 20mer (GA)10 formed a homoduplex in neutral solutions...
Conformational properties of DNA dodecamers containing four tandem repeats of the CNG triplets.
Vorlícková, M, Zimulová, M, Kovanda, J, Fojtík, P, Kypr, J
We studied DNA dodecamers (CAG)4, (CCG)4, (CGG)4 and (CTG)4by CD spectroscopy and polyacrylamide gel electrophoresis. Each dodecamer adopted several ordered conformers which denatured in a...
Millimolar concentrations of zinc and other metal cations cause sedimentation of DNA.
We demonstrate that DNA sediments in the presence of millimolar concentrations of zinc or related metal cations and that EDTA entirely dissolves the sediment. The sedimentation is promoted by...
Dimerization of the guanine-adenine repeat strands of DNA.
Vorlícková, M, Kejnovská, I, Kovanda, J, Kypr, J
Jovin and co-workers have demonstrated that DNA strands containing guanine-adenine repeats generate a parallel-stranded homoduplex. Here we propose that the homoduplex is a dimer of the ordered...
Aqueous trifluorethanol solutions simulate the environment of DNA in the crystalline state.
Kypr, J, Chládková, J, Zimulová, M, Vorlícková, M
We took 28 fragments of DNA whose crystal structures were known and used CD spectroscopy to search for conditions stabilising the crystal structures in solution. All 28 fragments switched into their...
Conformational transitions of poly(dA-bromo5dU) and poly(dA-iodo5dU) in solution.
Vorlícková, M, Chládková, J, Kypr, J
Extensive circular dichroism studies have been conducted with the title polynucleotides under various solution conditions. The studies provided the following information: (i) The halogen atoms in...
Salt-induced conformational changes of poly(dA-dT).
Vorlícková, M, Kypr, J, Kleinwächter, V, Palecek, E
Conformational changes of poly(dA-dT) . poly(dA-dT) induced by increasing ionic strength were studied using CD spectroscopy. It was found that a pronounced noncooperative inversion of the...
A Z-like form of poly(dA-dC).poly(dG-dT) in solution?
Vorlíckovă, M, Kypr, J, Stokrová, S, Sponar, J
Circular dichroism was used to study changes in conformation of poly(dA-dC).poly(dG-dT) caused by a high concentration of various monovalent salts. It was found that CsF induced the gradual...
Conformational transitions of poly(dA-dT)poly(dA-dT) in ethanolic solutions.
Vorlícková, M, Sedlácek, P, Kypr, J, Sponar, J
Examination of circular dichroic and phosphorus nuclear magnetic resonance spectra showed that poly(dA-dT)-poly(dA-dT) exhibited an ethanol-induced transition to the A form in an Na+ containing...
Vorlícková, M, Sági, J, Szabolcs, A, Szemzö, A, Otvös, L, Kypr, J
It has previously been demonstrated by other workers that the duplex of a synthetic DNA poly(amino2dA-dT) undergoes a salt-induced conformational isomerization. We show in the present work using...
Comparison of the solution and crystal conformations of (G + C)-rich fragments of DNA.
Vorlícková, M, Subirana, J A, Chládková, J, Tejralová, I, Huynh-Dinh, T, Arnold, L, ...
DNA fragments crystallize in an unpredictable manner, and relationships between their crystal and solution conformations still are not known. We have studied, using circular dichroism spectroscopy,...
A fast, very simple and efficient method of DNA extraction is described which takes advantage of DNA sedimentation induced by millimolar concentrations of ZnCl2. The zinc-induced sedimentation is...
Conformational properties of DNA strands containing guanine-adenine and thymine-adenine repeats.
Vorlicková, M, Kejnovská, I, Kovanda, J, Kypr, J
CD spectroscopy and PAGE were used to cooperatively analyze melting conformers of DNA strands containing GA and TA dinucleotide repeats. The 20mer (GA)10 formed a homoduplex in neutral solutions...
Conformational properties of DNA dodecamers containing four tandem repeats of the CNG triplets.
Vorlícková, M, Zimulová, M, Kovanda, J, Fojtík, P, Kypr, J
We studied DNA dodecamers (CAG)4, (CCG)4, (CGG)4 and (CTG)4by CD spectroscopy and polyacrylamide gel electrophoresis. Each dodecamer adopted several ordered conformers which denatured in a...
Millimolar concentrations of zinc and other metal cations cause sedimentation of DNA.
We demonstrate that DNA sediments in the presence of millimolar concentrations of zinc or related metal cations and that EDTA entirely dissolves the sediment. The sedimentation is promoted by...
Dimerization of the guanine-adenine repeat strands of DNA.
Vorlícková, M, Kejnovská, I, Kovanda, J, Kypr, J
Jovin and co-workers have demonstrated that DNA strands containing guanine-adenine repeats generate a parallel-stranded homoduplex. Here we propose that the homoduplex is a dimer of the ordered...
Aqueous trifluorethanol solutions simulate the environment of DNA in the crystalline state.
Kypr, J, Chládková, J, Zimulová, M, Vorlícková, M
We took 28 fragments of DNA whose crystal structures were known and used CD spectroscopy to search for conditions stabilising the crystal structures in solution. All 28 fragments switched into their...
Conformational transitions of poly(dA-bromo5dU) and poly(dA-iodo5dU) in solution.
Vorlícková, M, Chládková, J, Kypr, J
Extensive circular dichroism studies have been conducted with the title polynucleotides under various solution conditions. The studies provided the following information: (i) The halogen atoms in...
Salt-induced conformational changes of poly(dA-dT).
Vorlícková, M, Kypr, J, Kleinwächter, V, Palecek, E
Conformational changes of poly(dA-dT) . poly(dA-dT) induced by increasing ionic strength were studied using CD spectroscopy. It was found that a pronounced noncooperative inversion of the...
A Z-like form of poly(dA-dC).poly(dG-dT) in solution?
Vorlíckovă, M, Kypr, J, Stokrová, S, Sponar, J
Circular dichroism was used to study changes in conformation of poly(dA-dC).poly(dG-dT) caused by a high concentration of various monovalent salts. It was found that CsF induced the gradual...
Conformational transitions of poly(dA-dT)poly(dA-dT) in ethanolic solutions.
Vorlícková, M, Sedlácek, P, Kypr, J, Sponar, J
Examination of circular dichroic and phosphorus nuclear magnetic resonance spectra showed that poly(dA-dT)-poly(dA-dT) exhibited an ethanol-induced transition to the A form in an Na+ containing...
Vorlícková, M, Sági, J, Szabolcs, A, Szemzö, A, Otvös, L, Kypr, J
It has previously been demonstrated by other workers that the duplex of a synthetic DNA poly(amino2dA-dT) undergoes a salt-induced conformational isomerization. We show in the present work using...
Comparison of the solution and crystal conformations of (G + C)-rich fragments of DNA.
Vorlícková, M, Subirana, J A, Chládková, J, Tejralová, I, Huynh-Dinh, T, Arnold, L, ...
DNA fragments crystallize in an unpredictable manner, and relationships between their crystal and solution conformations still are not known. We have studied, using circular dichroism spectroscopy,...