Freedman, J. E., Loscalzo, J., Benoit, S. E., Valeri, C. R., Barnard, M. R.
Highly reactive oxygen species rapidly inactivate nitric oxide (NO), an endothelial product which inhibits platelet activation. We studied platelet inhibition by NO in two brothers with a cerebral...
Khuri, S. F., Valeri, C. R., Loscalzo, J., Weinstein, M., Birjiniuk, V.
Platelet dysfunction and increased fibrinolysis are the most important etiologic factors in the hemostatic defect observed following the institution of cardiopulmonary bypass. This study examined the...
Lieberthal, W., Fuhro, R., Freedman, J. E., Toolan, G., Loscalzo, J.
We studied the hemodynamic effects of a 20% exchange transfusion with whole blood or highly purified human hemoglobin Ao. We compared the effects of unmodified hemoglobin with o-raffinose crosslinked...
The Platelet Function Defect of Cardiopulmonary Bypass. (1998)
KESTIN, Anita S., VALERI, C.R., KHURI, S.F., LOSCALZO, J., ELLIS, P.A.
The use of cardiopulmonary bypass (CPB) during cardiac surgery is associated with a hemostatic defect, the hallmark of which is a markedly prolonged bleeding time. However, the nature of the putative...
Michelson, A D, Barnard, M R, Hechtman, H B, MacGregor, H, Connolly, R J, Loscalzo, J, ...
To examine the hypothesis that surface P-selectin-positive (degranulated) platelets are rapidly cleared from the circulation, we developed novel methods for tracking of platelets and measurement of...
Salivary gland extracts from the deerfly contain a potent inhibitor of platelet aggregation.
Grevelink, S A, Youssef, D E, Loscalzo, J, Lerner, E A
Salivary gland extracts of the deerfly contain a potent inhibitor of platelet aggregation, which assists the insect in obtaining a blood meal. The extract prevents platelet aggregation induced by...
Dietary antioxidants preserve endothelium-dependent vessel relaxation in cholesterol-fed rabbits.
Keaney, J F, Gaziano, J M, Xu, A, Frei, B, Curran-Celentano, J, Shwaery, G T, ...
Recent evidence suggests that dietary therapy with lipid-soluble antioxidants may be beneficial for patients with atherosclerotic vascular disease but the potential mechanism(s) for these...
Stamler, J S, Simon, D I, Osborne, J A, Mullins, M E, Jaraki, O, Michel, T, ...
Endothelium-derived relaxing factor (EDRF) activity has been attributed to the highly labile nitric oxide radical (NO). In view of the fact that the plasma and cellular milieux contain reactive...
Nitric oxide circulates in mammalian plasma primarily as an S-nitroso adduct of serum albumin.
Stamler, J S, Jaraki, O, Osborne, J, Simon, D I, Keaney, J, Vita, J, ...
We have recently shown that nitric oxide or authentic endothelium-derived relaxing factor generated in a biologic system reacts in the presence of specific protein thiols to form S-nitrosoprotein...
Stamler, J S, Simon, D I, Jaraki, O, Osborne, J A, Francis, S, Mullins, M, ...
Tissue-type plasminogen activator (t-PA) reacts upon exposure to endothelium-derived relaxing factor (EDRF) by way of the enzyme's single free sulfhydryl (Cys-83) to form a stable S-nitrosothiol...
Glutathione peroxidase potentiates the inhibition of platelet function by S-nitrosothiols.
Freedman, J E, Frei, B, Welch, G N, Loscalzo, J
GSH peroxidase (Px) catalyzes the reduction of lipid hydroperoxides (LOOH), known metabolic products of platelets and vascular cells. Because interactions between these cells are modulated by nitric...
Marks, D S, Vita, J A, Folts, J D, Keaney, J F, Welch, G N, Loscalzo, J
Endothelium-derived relaxing factor is important for vascular homeostasis and possesses qualities that may modulate vascular injury, including vasodilation, platelet inhibition, and inhibition of...
NO forms an adduct with serum albumin that has endothelium-derived relaxing factor-like properties.
Keaney, J F, Simon, D I, Stamler, J S, Jaraki, O, Scharfstein, J, Vita, J A, ...
Recent evidence suggests that sulfhydryl species can react with oxides of nitrogen under physiologic conditions and thereby stabilize endothelium-derived relaxing factor (EDRF) activity, but the...
Keaney, J F, Gaziano, J M, Xu, A, Frei, B, Curran-Celentano, J, Shwaery, G T, ...
Abnormalities in endothelium-dependent arterial relaxation develop early in atherosclerosis and may, in part, result from the effects of modified low-density lipoprotein (LDL) on agonist-mediated...
Slivka, A, Chuttani, R, Carr-Locke, D L, Kobzik, L, Bredt, D S, Loscalzo, J, ...
Nitric oxide (NO) is an inhibitor of gastrointestinal smooth muscle. Model systems of the gut predict the NO will complex with biological thiol (SH) groups, yielding S-nitrosothiols (RS-NO), which...
Scharfstein, J S, Keaney, J F, Slivka, A, Welch, G N, Vita, J A, Stamler, J S, ...
Plasma albumin reacts with nitric oxide (NO) to form the bioactive adduct, S-nitroso-albumin (S-NO-albumin). The limited intracellular access of S-NO-albumin suggests the need for a vascular transfer...
Hare, J M, Keaney, J F, Balligand, J L, Loscalzo, J, Smith, T W, Colucci, W S
In vitro studies indicate that muscarinic cholinergic inhibition of beta-adrenergic cardiac responses may be modulated in part by nitric oxide (NO). To evaluate the role of NO in parasympathetic...
Flow activates an endothelial potassium channel to release an endogenous nitrovasodilator.
Cooke, J P, Rossitch, E, Andon, N A, Loscalzo, J, Dzau, V J
Flow-mediated vasodilation is endothelium dependent. We hypothesized that flow activates a potassium channel on the endothelium, and that activation of this channel leads to the release of the...
Impaired vasodilation of forearm resistance vessels in hypercholesterolemic humans.
Creager, M A, Cooke, J P, Mendelsohn, M E, Gallagher, S J, Coleman, S M, Loscalzo, J, ...
The effect of hypercholesterolemia on vascular function was studied in humans. To eliminate the potential confounding effects of atherosclerosis, vascular reactivity was measured in the forearm...
Endothelial injury, vasoconstriction, and its prevention.
The endothelial cell is an important determinant of vascular tone, and performs this function by the elaboration of several factors that modulate the contractile state of the vascular smooth muscle...
Stamler, J S, Osborne, J A, Jaraki, O, Rabbani, L E, Mullins, M, Singel, D, ...
Elevated levels of homocysteine are associated with an increased risk of atherosclerosis and thrombosis. The reactivity of the sulfhydryl group of homocysteine has been implicated in molecular...
von Willebrand protein facilitates platelet incorporation in polymerizing fibrin.
Loscalzo, J, Inbal, A, Handin, R I
von Willebrand protein was found to promote the incorporation of platelets into evolving fibrin thrombi. Using formalin-treated or fresh platelets, both the initial rate and extent of platelet...
N-Acetylcysteine potentiates inhibition of platelet aggregation by nitroglycerin.
Platelet aggregation is currently felt to play an important role in the pathogenesis of ischemic vascular disorders. The smooth muscle relaxant, nitroglycerin, has been shown to inhibit platelet...
Tissue plasminogen activator promotes platelet disaggregation in plasma.
We studied the disaggregation of human platelets by tissue-type plasminogen activator (t-PA). When added to a suspension of human platelets induced to aggregate in plasma with adenosine...
Conformational change and cooperativity in actin filaments free of tropomyosin.
Loscalzo, J, Reed, G H, Weber, A
The decrease in amplitude of the electron spin resonance spectrum of the cysteine-bound spin-label, 3-(maleimidomethyl)-2,2,5,5-tetramethyl-1-pyrrolidinoxyl, brought about by the magnetic interaction...
Role of platelets in cholesteryl ester formation by U-937 cells.
The conversion of tissue macrophages into cholesteryl ester-rich foam cells is a crucial early event in atherogenesis. We studied the platelet as a potential source of cholesterol for esterification...
We examined the similarities and differences in conformation between recombinant human single-chain tissue plasminogen activator (sct-PA) and two-chain tissue plasminogen activator (tct-PA), and...
Decreased platelet inhibition by nitric oxide in two brothers with a history of arterial thrombosis.
Freedman, J E, Loscalzo, J, Benoit, S E, Valeri, C R, Barnard, M R, Michelson, A D
Highly reactive oxygen species rapidly inactivate nitric oxide (NO), and endothelial product which inhibits platelet activation. We studied platelet inhibition by NO in two brothers with a cerebral...
Nitric oxide released from activated platelets inhibits platelet recruitment.
Freedman, J E, Loscalzo, J, Barnard, M R, Alpert, C, Keaney, J F, Michelson, A D
Vessel injury and thrombus formation are the cause of most ischemic coronary syndromes and, in this setting, activated platelets stimulate platelet recruitment to the growing thrombus. Recently, a...
Zhang, Y Y, Hammarberg, T, Radmark, O, Samuelsson, B, Ng, C F, Funk, C D, ...
5-Lipoxygenase (5LO) catalyses the first two steps in the biosynthesis of leukotrienes, which are inflammatory mediators derived from arachidonic acid. 5LO activity is stimulated by ATP; however, a...
Michelson, A D, Barnard, M R, Hechtman, H B, MacGregor, H, Connolly, R J, Loscalzo, J, ...
To examine the hypothesis that surface P-selectin-positive (degranulated) platelets are rapidly cleared from the circulation, we developed novel methods for tracking of platelets and measurement of...
Salivary gland extracts from the deerfly contain a potent inhibitor of platelet aggregation.
Grevelink, S A, Youssef, D E, Loscalzo, J, Lerner, E A
Salivary gland extracts of the deerfly contain a potent inhibitor of platelet aggregation, which assists the insect in obtaining a blood meal. The extract prevents platelet aggregation induced by...
Dietary antioxidants preserve endothelium-dependent vessel relaxation in cholesterol-fed rabbits.
Keaney, J F, Gaziano, J M, Xu, A, Frei, B, Curran-Celentano, J, Shwaery, G T, ...
Recent evidence suggests that dietary therapy with lipid-soluble antioxidants may be beneficial for patients with atherosclerotic vascular disease but the potential mechanism(s) for these...
Stamler, J S, Simon, D I, Osborne, J A, Mullins, M E, Jaraki, O, Michel, T, ...
Endothelium-derived relaxing factor (EDRF) activity has been attributed to the highly labile nitric oxide radical (NO). In view of the fact that the plasma and cellular milieux contain reactive...
Nitric oxide circulates in mammalian plasma primarily as an S-nitroso adduct of serum albumin.
Stamler, J S, Jaraki, O, Osborne, J, Simon, D I, Keaney, J, Vita, J, ...
We have recently shown that nitric oxide or authentic endothelium-derived relaxing factor generated in a biologic system reacts in the presence of specific protein thiols to form S-nitrosoprotein...
Stamler, J S, Simon, D I, Jaraki, O, Osborne, J A, Francis, S, Mullins, M, ...
Tissue-type plasminogen activator (t-PA) reacts upon exposure to endothelium-derived relaxing factor (EDRF) by way of the enzyme's single free sulfhydryl (Cys-83) to form a stable S-nitrosothiol...
Glutathione peroxidase potentiates the inhibition of platelet function by S-nitrosothiols.
Freedman, J E, Frei, B, Welch, G N, Loscalzo, J
GSH peroxidase (Px) catalyzes the reduction of lipid hydroperoxides (LOOH), known metabolic products of platelets and vascular cells. Because interactions between these cells are modulated by nitric...
Marks, D S, Vita, J A, Folts, J D, Keaney, J F, Welch, G N, Loscalzo, J
Endothelium-derived relaxing factor is important for vascular homeostasis and possesses qualities that may modulate vascular injury, including vasodilation, platelet inhibition, and inhibition of...
NO forms an adduct with serum albumin that has endothelium-derived relaxing factor-like properties.
Keaney, J F, Simon, D I, Stamler, J S, Jaraki, O, Scharfstein, J, Vita, J A, ...
Recent evidence suggests that sulfhydryl species can react with oxides of nitrogen under physiologic conditions and thereby stabilize endothelium-derived relaxing factor (EDRF) activity, but the...
Keaney, J F, Gaziano, J M, Xu, A, Frei, B, Curran-Celentano, J, Shwaery, G T, ...
Abnormalities in endothelium-dependent arterial relaxation develop early in atherosclerosis and may, in part, result from the effects of modified low-density lipoprotein (LDL) on agonist-mediated...
Slivka, A, Chuttani, R, Carr-Locke, D L, Kobzik, L, Bredt, D S, Loscalzo, J, ...
Nitric oxide (NO) is an inhibitor of gastrointestinal smooth muscle. Model systems of the gut predict the NO will complex with biological thiol (SH) groups, yielding S-nitrosothiols (RS-NO), which...
Scharfstein, J S, Keaney, J F, Slivka, A, Welch, G N, Vita, J A, Stamler, J S, ...
Plasma albumin reacts with nitric oxide (NO) to form the bioactive adduct, S-nitroso-albumin (S-NO-albumin). The limited intracellular access of S-NO-albumin suggests the need for a vascular transfer...
Hare, J M, Keaney, J F, Balligand, J L, Loscalzo, J, Smith, T W, Colucci, W S
In vitro studies indicate that muscarinic cholinergic inhibition of beta-adrenergic cardiac responses may be modulated in part by nitric oxide (NO). To evaluate the role of NO in parasympathetic...
Flow activates an endothelial potassium channel to release an endogenous nitrovasodilator.
Cooke, J P, Rossitch, E, Andon, N A, Loscalzo, J, Dzau, V J
Flow-mediated vasodilation is endothelium dependent. We hypothesized that flow activates a potassium channel on the endothelium, and that activation of this channel leads to the release of the...
Impaired vasodilation of forearm resistance vessels in hypercholesterolemic humans.
Creager, M A, Cooke, J P, Mendelsohn, M E, Gallagher, S J, Coleman, S M, Loscalzo, J, ...
The effect of hypercholesterolemia on vascular function was studied in humans. To eliminate the potential confounding effects of atherosclerosis, vascular reactivity was measured in the forearm...
Endothelial injury, vasoconstriction, and its prevention.
The endothelial cell is an important determinant of vascular tone, and performs this function by the elaboration of several factors that modulate the contractile state of the vascular smooth muscle...
Stamler, J S, Osborne, J A, Jaraki, O, Rabbani, L E, Mullins, M, Singel, D, ...
Elevated levels of homocysteine are associated with an increased risk of atherosclerosis and thrombosis. The reactivity of the sulfhydryl group of homocysteine has been implicated in molecular...
von Willebrand protein facilitates platelet incorporation in polymerizing fibrin.
Loscalzo, J, Inbal, A, Handin, R I
von Willebrand protein was found to promote the incorporation of platelets into evolving fibrin thrombi. Using formalin-treated or fresh platelets, both the initial rate and extent of platelet...
N-Acetylcysteine potentiates inhibition of platelet aggregation by nitroglycerin.
Platelet aggregation is currently felt to play an important role in the pathogenesis of ischemic vascular disorders. The smooth muscle relaxant, nitroglycerin, has been shown to inhibit platelet...
Tissue plasminogen activator promotes platelet disaggregation in plasma.
We studied the disaggregation of human platelets by tissue-type plasminogen activator (t-PA). When added to a suspension of human platelets induced to aggregate in plasma with adenosine...
Conformational change and cooperativity in actin filaments free of tropomyosin.
Loscalzo, J, Reed, G H, Weber, A
The decrease in amplitude of the electron spin resonance spectrum of the cysteine-bound spin-label, 3-(maleimidomethyl)-2,2,5,5-tetramethyl-1-pyrrolidinoxyl, brought about by the magnetic interaction...
Role of platelets in cholesteryl ester formation by U-937 cells.
The conversion of tissue macrophages into cholesteryl ester-rich foam cells is a crucial early event in atherogenesis. We studied the platelet as a potential source of cholesterol for esterification...
We examined the similarities and differences in conformation between recombinant human single-chain tissue plasminogen activator (sct-PA) and two-chain tissue plasminogen activator (tct-PA), and...
Decreased platelet inhibition by nitric oxide in two brothers with a history of arterial thrombosis.
Freedman, J E, Loscalzo, J, Benoit, S E, Valeri, C R, Barnard, M R, Michelson, A D
Highly reactive oxygen species rapidly inactivate nitric oxide (NO), and endothelial product which inhibits platelet activation. We studied platelet inhibition by NO in two brothers with a cerebral...
Nitric oxide released from activated platelets inhibits platelet recruitment.
Freedman, J E, Loscalzo, J, Barnard, M R, Alpert, C, Keaney, J F, Michelson, A D
Vessel injury and thrombus formation are the cause of most ischemic coronary syndromes and, in this setting, activated platelets stimulate platelet recruitment to the growing thrombus. Recently, a...
Zhang, Y Y, Hammarberg, T, Radmark, O, Samuelsson, B, Ng, C F, Funk, C D, ...
5-Lipoxygenase (5LO) catalyses the first two steps in the biosynthesis of leukotrienes, which are inflammatory mediators derived from arachidonic acid. 5LO activity is stimulated by ATP; however, a...
Nitric oxide insufficiency and arterial thrombosis.
Loscalzo, J., Freedman, J., Inbal, A., Keaney, J. F., Michelson, A. D., Vita, J. A.
Mechanisms of oxidative stress and vascular dysfunction
Nedeljkovic, Z, Gokce, N, Loscalzo, J
The endothelium regulates vascular homoeostasis through local elaboration of mediators that modulate vascular tone, platelet adhesion, inflammation, fibrinolysis, and vascular growth. Impaired...